Coniochaeta sp. 2T2.1
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24540 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5N5LX49|A0A5N5LX49_9PEZI Uncharacterized protein OS=Coniochaeta sp. 2T2.1 OX=1571157 GN=GE09DRAFT_1060439 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 9.2 FTASLPALFAATVLVLAPALVKK24 pKa = 10.52 AWDD27 pKa = 3.74 FEE29 pKa = 4.99 VYY31 pKa = 10.65 DD32 pKa = 4.3 EE33 pKa = 5.32 DD34 pKa = 5.85 CVTSLDD40 pKa = 4.14 KK41 pKa = 11.59 YY42 pKa = 11.0 SGTGDD47 pKa = 3.73 VEE49 pKa = 4.7 CDD51 pKa = 3.38 DD52 pKa = 4.34 VVEE55 pKa = 5.07 GDD57 pKa = 5.05 DD58 pKa = 5.8 DD59 pKa = 3.9 EE60 pKa = 7.25 CEE62 pKa = 4.6 DD63 pKa = 4.94 GDD65 pKa = 4.84 VLDD68 pKa = 5.96 DD69 pKa = 3.51 YY70 pKa = 11.05 TAADD74 pKa = 4.09 EE75 pKa = 4.79 GRR77 pKa = 11.84 DD78 pKa = 3.57 ITDD81 pKa = 3.6 HH82 pKa = 6.75 EE83 pKa = 4.78 DD84 pKa = 3.38 FRR86 pKa = 11.84 SHH88 pKa = 6.97 SVHH91 pKa = 6.73 CC92 pKa = 4.58
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.439
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.35
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.05
Thurlkill 3.516
EMBOSS 3.643
Sillero 3.795
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|A0A5N5MIA1|A0A5N5MIA1_9PEZI Uncharacterized protein OS=Coniochaeta sp. 2T2.1 OX=1571157 GN=GE09DRAFT_140083 PE=4 SV=1
MM1 pKa = 7.69 RR2 pKa = 11.84 GARR5 pKa = 11.84 SSRR8 pKa = 11.84 PRR10 pKa = 11.84 TAKK13 pKa = 10.51 SPPTPARR20 pKa = 11.84 ARR22 pKa = 11.84 VKK24 pKa = 10.57 AKK26 pKa = 10.17 LRR28 pKa = 11.84 ARR30 pKa = 11.84 AKK32 pKa = 9.49 RR33 pKa = 11.84 AARR36 pKa = 11.84 RR37 pKa = 11.84 ARR39 pKa = 11.84 AKK41 pKa = 9.92 VRR43 pKa = 11.84 RR44 pKa = 11.84 AARR47 pKa = 11.84 LLLPASLRR55 pKa = 11.84 EE56 pKa = 4.03 ARR58 pKa = 11.84 ARR60 pKa = 11.84 AARR63 pKa = 11.84 SNKK66 pKa = 8.77 PRR68 pKa = 11.84 TARR71 pKa = 11.84 SLLQEE76 pKa = 3.8 RR77 pKa = 11.84 VRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 KK86 pKa = 9.77 RR87 pKa = 11.84 KK88 pKa = 7.41 MTASPTGSRR97 pKa = 11.84 TTSPVSTDD105 pKa = 3.28 SAPPTPAASSLARR118 pKa = 11.84 SLLGWPASRR127 pKa = 11.84 PVASAAARR135 pKa = 11.84 SAGLSCRR142 pKa = 11.84 SRR144 pKa = 11.84 SSPRR148 pKa = 11.84 LDD150 pKa = 2.73 IDD152 pKa = 3.57 MVNN155 pKa = 3.15
Molecular weight: 16.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.405
IPC2_protein 10.877
IPC_protein 12.471
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.632
Wikipedia 13.115
Rodwell 12.237
Grimsley 12.676
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.632
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 11.945
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.131
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24540
0
24540
11563170
50
7695
471.2
51.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.052 ± 0.015
1.261 ± 0.006
5.707 ± 0.012
6.015 ± 0.016
3.598 ± 0.009
7.382 ± 0.015
2.404 ± 0.006
4.438 ± 0.009
4.619 ± 0.015
8.723 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.006
3.438 ± 0.009
6.372 ± 0.017
3.882 ± 0.013
6.281 ± 0.013
7.929 ± 0.018
6.132 ± 0.013
6.407 ± 0.012
1.501 ± 0.007
2.711 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here