Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4821 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q87LN6|Q87LN6_VIBPA Sigma-E factor regulatory protein RseC OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=VP2575 PE=4 SV=1
MM1 pKa = 7.41 SHH3 pKa = 6.51 EE4 pKa = 4.59 DD5 pKa = 3.36 EE6 pKa = 4.41 YY7 pKa = 11.8 LSVEE11 pKa = 4.13 EE12 pKa = 5.07 LIEE15 pKa = 4.01 IQKK18 pKa = 10.79 EE19 pKa = 3.96 EE20 pKa = 4.06 TRR22 pKa = 11.84 DD23 pKa = 3.25 IIAALLEE30 pKa = 5.14 DD31 pKa = 4.8 GSDD34 pKa = 3.49 PDD36 pKa = 3.36 SLYY39 pKa = 10.83 EE40 pKa = 4.15 IEE42 pKa = 4.37 HH43 pKa = 6.59 HH44 pKa = 7.1 LFAEE48 pKa = 5.16 DD49 pKa = 4.33 FDD51 pKa = 4.45 TLEE54 pKa = 4.3 KK55 pKa = 10.85 AVVEE59 pKa = 4.0 AFKK62 pKa = 10.62 MGFEE66 pKa = 4.02 VLEE69 pKa = 4.38 AEE71 pKa = 4.32 EE72 pKa = 4.84 TEE74 pKa = 5.32 DD75 pKa = 4.16 EE76 pKa = 4.91 DD77 pKa = 5.29 GNKK80 pKa = 9.92 LLCCDD85 pKa = 3.51 ATMEE89 pKa = 4.41 SALNAEE95 pKa = 4.98 AIDD98 pKa = 3.82 AQVEE102 pKa = 4.49 KK103 pKa = 10.53 LVHH106 pKa = 5.94 LAEE109 pKa = 5.18 KK110 pKa = 10.82 YY111 pKa = 10.77 DD112 pKa = 3.78 IIYY115 pKa = 10.15 DD116 pKa = 3.64 GWGTYY121 pKa = 9.39 YY122 pKa = 10.49 EE123 pKa = 5.11 GEE125 pKa = 4.09 DD126 pKa = 3.63 ALYY129 pKa = 10.83 SDD131 pKa = 5.46 EE132 pKa = 5.71 DD133 pKa = 4.72 DD134 pKa = 3.79 EE135 pKa = 6.94 DD136 pKa = 3.83 EE137 pKa = 4.42
Molecular weight: 15.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.681
IPC_protein 3.668
Toseland 3.465
ProMoST 3.783
Dawson 3.63
Bjellqvist 3.808
Wikipedia 3.528
Rodwell 3.49
Grimsley 3.376
Solomon 3.617
Lehninger 3.579
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.503
EMBOSS 3.541
Sillero 3.783
Patrickios 1.062
IPC_peptide 3.617
IPC2_peptide 3.757
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|Q87RZ0|Q87RZ0_VIBPA Uncharacterized protein OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=VP0634 PE=4 SV=1
MM1 pKa = 7.66 RR2 pKa = 11.84 RR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 VQSKK9 pKa = 10.26 RR10 pKa = 11.84 LNKK13 pKa = 8.61 TVAAIRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 10.1 RR22 pKa = 11.84 KK23 pKa = 8.14 LTTMKK28 pKa = 10.52 KK29 pKa = 9.88 KK30 pKa = 9.26 VSSRR34 pKa = 11.84 NRR36 pKa = 11.84 VFVHH40 pKa = 6.24 LVNHH44 pKa = 6.96
Molecular weight: 5.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4821
0
4821
1490746
14
6084
309.2
34.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.565 ± 0.035
1.045 ± 0.013
5.502 ± 0.042
6.542 ± 0.042
4.201 ± 0.029
6.776 ± 0.037
2.242 ± 0.019
6.18 ± 0.028
5.385 ± 0.035
10.187 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.739 ± 0.024
4.265 ± 0.026
3.892 ± 0.023
4.534 ± 0.031
4.475 ± 0.035
6.587 ± 0.03
5.406 ± 0.042
7.196 ± 0.033
1.24 ± 0.014
3.042 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here