Amino acid dipepetide frequency for Pseudomonas phage phi15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.723AlaAla: 10.723 ± 1.083
0.731AlaCys: 0.731 ± 0.221
5.768AlaAsp: 5.768 ± 0.88
5.199AlaGlu: 5.199 ± 0.846
3.249AlaPhe: 3.249 ± 0.455
7.149AlaGly: 7.149 ± 0.9
1.219AlaHis: 1.219 ± 0.359
4.224AlaIle: 4.224 ± 0.565
6.174AlaLys: 6.174 ± 0.595
10.723AlaLeu: 10.723 ± 1.108
3.574AlaMet: 3.574 ± 0.576
3.331AlaAsn: 3.331 ± 0.508
3.249AlaPro: 3.249 ± 0.624
4.549AlaGln: 4.549 ± 0.688
5.686AlaArg: 5.686 ± 0.772
5.93AlaSer: 5.93 ± 0.703
5.686AlaThr: 5.686 ± 1.115
6.418AlaVal: 6.418 ± 0.667
1.3AlaTrp: 1.3 ± 0.598
3.412AlaTyr: 3.412 ± 0.679
0.0AlaXaa: 0.0 ± 0.0
Cys
0.65CysAla: 0.65 ± 0.21
0.0CysCys: 0.0 ± 0.0
0.65CysAsp: 0.65 ± 0.289
0.975CysGlu: 0.975 ± 0.312
0.569CysPhe: 0.569 ± 0.251
0.731CysGly: 0.731 ± 0.228
0.244CysHis: 0.244 ± 0.13
0.65CysIle: 0.65 ± 0.22
0.487CysLys: 0.487 ± 0.183
0.65CysLeu: 0.65 ± 0.191
0.325CysMet: 0.325 ± 0.205
0.406CysAsn: 0.406 ± 0.169
0.406CysPro: 0.406 ± 0.167
0.406CysGln: 0.406 ± 0.199
0.731CysArg: 0.731 ± 0.263
0.487CysSer: 0.487 ± 0.163
0.487CysThr: 0.487 ± 0.186
0.569CysVal: 0.569 ± 0.216
0.0CysTrp: 0.0 ± 0.0
0.244CysTyr: 0.244 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
6.418AspAla: 6.418 ± 1.059
0.65AspCys: 0.65 ± 0.227
4.793AspAsp: 4.793 ± 0.689
3.656AspGlu: 3.656 ± 0.492
2.6AspPhe: 2.6 ± 0.379
6.093AspGly: 6.093 ± 0.647
1.137AspHis: 1.137 ± 0.3
3.737AspIle: 3.737 ± 0.5
2.112AspLys: 2.112 ± 0.426
5.037AspLeu: 5.037 ± 0.583
1.3AspMet: 1.3 ± 0.274
2.356AspAsn: 2.356 ± 0.39
3.168AspPro: 3.168 ± 0.699
2.193AspGln: 2.193 ± 0.4
3.981AspArg: 3.981 ± 0.582
3.331AspSer: 3.331 ± 0.648
2.681AspThr: 2.681 ± 0.426
3.737AspVal: 3.737 ± 0.582
1.219AspTrp: 1.219 ± 0.305
2.031AspTyr: 2.031 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
8.448GluAla: 8.448 ± 1.008
0.894GluCys: 0.894 ± 0.293
4.468GluAsp: 4.468 ± 0.702
4.712GluGlu: 4.712 ± 0.722
2.518GluPhe: 2.518 ± 0.417
5.037GluGly: 5.037 ± 0.774
2.193GluHis: 2.193 ± 0.533
2.193GluIle: 2.193 ± 0.3
2.6GluLys: 2.6 ± 0.406
5.605GluLeu: 5.605 ± 0.615
2.437GluMet: 2.437 ± 0.394
2.356GluAsn: 2.356 ± 0.554
2.518GluPro: 2.518 ± 0.524
3.412GluGln: 3.412 ± 0.805
4.143GluArg: 4.143 ± 0.563
3.493GluSer: 3.493 ± 0.522
4.305GluThr: 4.305 ± 0.687
4.793GluVal: 4.793 ± 0.739
0.975GluTrp: 0.975 ± 0.344
2.112GluTyr: 2.112 ± 0.422
0.0GluXaa: 0.0 ± 0.0
Phe
2.193PheAla: 2.193 ± 0.362
0.731PheCys: 0.731 ± 0.256
2.843PheAsp: 2.843 ± 0.541
2.112PheGlu: 2.112 ± 0.439
1.3PhePhe: 1.3 ± 0.287
2.843PheGly: 2.843 ± 0.401
1.219PheHis: 1.219 ± 0.302
2.193PheIle: 2.193 ± 0.629
2.193PheLys: 2.193 ± 0.373
3.412PheLeu: 3.412 ± 0.581
1.056PheMet: 1.056 ± 0.318
2.031PheAsn: 2.031 ± 0.425
1.3PhePro: 1.3 ± 0.352
0.975PheGln: 0.975 ± 0.299
2.356PheArg: 2.356 ± 0.425
2.275PheSer: 2.275 ± 0.462
2.518PheThr: 2.518 ± 0.512
2.031PheVal: 2.031 ± 0.327
0.65PheTrp: 0.65 ± 0.231
0.812PheTyr: 0.812 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
7.149GlyAla: 7.149 ± 1.402
0.65GlyCys: 0.65 ± 0.212
5.93GlyAsp: 5.93 ± 0.622
5.605GlyGlu: 5.605 ± 0.846
3.493GlyPhe: 3.493 ± 0.457
6.336GlyGly: 6.336 ± 0.796
2.275GlyHis: 2.275 ± 0.475
4.468GlyIle: 4.468 ± 0.69
5.686GlyLys: 5.686 ± 0.979
6.661GlyLeu: 6.661 ± 0.541
1.543GlyMet: 1.543 ± 0.442
3.168GlyAsn: 3.168 ± 0.535
1.95GlyPro: 1.95 ± 0.336
3.981GlyGln: 3.981 ± 0.554
4.549GlyArg: 4.549 ± 0.545
4.63GlySer: 4.63 ± 0.593
5.037GlyThr: 5.037 ± 0.727
5.768GlyVal: 5.768 ± 0.652
1.3GlyTrp: 1.3 ± 0.366
2.193GlyTyr: 2.193 ± 0.445
0.0GlyXaa: 0.0 ± 0.0
His
1.137HisAla: 1.137 ± 0.281
0.569HisCys: 0.569 ± 0.227
1.056HisAsp: 1.056 ± 0.383
2.031HisGlu: 2.031 ± 0.359
0.975HisPhe: 0.975 ± 0.315
1.625HisGly: 1.625 ± 0.346
0.325HisHis: 0.325 ± 0.16
0.975HisIle: 0.975 ± 0.375
0.894HisLys: 0.894 ± 0.242
2.275HisLeu: 2.275 ± 0.408
0.731HisMet: 0.731 ± 0.268
1.3HisAsn: 1.3 ± 0.322
0.731HisPro: 0.731 ± 0.289
0.731HisGln: 0.731 ± 0.29
1.219HisArg: 1.219 ± 0.36
0.812HisSer: 0.812 ± 0.282
0.812HisThr: 0.812 ± 0.196
1.543HisVal: 1.543 ± 0.332
0.406HisTrp: 0.406 ± 0.176
0.569HisTyr: 0.569 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
4.468IleAla: 4.468 ± 0.592
0.65IleCys: 0.65 ± 0.211
2.356IleAsp: 2.356 ± 0.486
3.331IleGlu: 3.331 ± 0.426
0.894IlePhe: 0.894 ± 0.277
3.656IleGly: 3.656 ± 0.56
0.569IleHis: 0.569 ± 0.258
1.787IleIle: 1.787 ± 0.354
3.493IleLys: 3.493 ± 0.546
4.143IleLeu: 4.143 ± 0.44
1.462IleMet: 1.462 ± 0.419
1.543IleAsn: 1.543 ± 0.418
2.112IlePro: 2.112 ± 0.336
1.95IleGln: 1.95 ± 0.378
3.737IleArg: 3.737 ± 0.42
1.787IleSer: 1.787 ± 0.451
3.006IleThr: 3.006 ± 0.547
3.574IleVal: 3.574 ± 0.493
0.894IleTrp: 0.894 ± 0.221
1.381IleTyr: 1.381 ± 0.442
0.0IleXaa: 0.0 ± 0.0
Lys
7.149LysAla: 7.149 ± 1.117
0.406LysCys: 0.406 ± 0.164
3.818LysAsp: 3.818 ± 0.652
5.037LysGlu: 5.037 ± 0.708
2.6LysPhe: 2.6 ± 0.424
4.305LysGly: 4.305 ± 0.786
1.219LysHis: 1.219 ± 0.384
1.95LysIle: 1.95 ± 0.412
2.924LysLys: 2.924 ± 0.78
3.981LysLeu: 3.981 ± 0.539
1.706LysMet: 1.706 ± 0.397
2.031LysAsn: 2.031 ± 0.478
2.6LysPro: 2.6 ± 0.452
2.356LysGln: 2.356 ± 0.445
3.574LysArg: 3.574 ± 0.646
3.331LysSer: 3.331 ± 0.573
2.275LysThr: 2.275 ± 0.519
4.305LysVal: 4.305 ± 0.751
0.812LysTrp: 0.812 ± 0.312
1.787LysTyr: 1.787 ± 0.338
0.0LysXaa: 0.0 ± 0.0
Leu
7.88LeuAla: 7.88 ± 0.696
0.325LeuCys: 0.325 ± 0.156
3.656LeuAsp: 3.656 ± 0.392
6.336LeuGlu: 6.336 ± 0.737
2.681LeuPhe: 2.681 ± 0.478
5.849LeuGly: 5.849 ± 0.785
1.625LeuHis: 1.625 ± 0.337
4.468LeuIle: 4.468 ± 0.54
6.336LeuLys: 6.336 ± 0.758
4.955LeuLeu: 4.955 ± 0.788
2.681LeuMet: 2.681 ± 0.484
4.468LeuAsn: 4.468 ± 0.777
3.087LeuPro: 3.087 ± 0.48
3.981LeuGln: 3.981 ± 0.768
5.361LeuArg: 5.361 ± 0.537
4.63LeuSer: 4.63 ± 0.706
6.011LeuThr: 6.011 ± 0.835
5.118LeuVal: 5.118 ± 0.608
0.975LeuTrp: 0.975 ± 0.261
1.95LeuTyr: 1.95 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
3.574MetAla: 3.574 ± 0.622
0.325MetCys: 0.325 ± 0.172
2.031MetAsp: 2.031 ± 0.343
1.95MetGlu: 1.95 ± 0.353
0.731MetPhe: 0.731 ± 0.224
1.787MetGly: 1.787 ± 0.451
0.406MetHis: 0.406 ± 0.159
1.137MetIle: 1.137 ± 0.289
1.706MetLys: 1.706 ± 0.319
1.868MetLeu: 1.868 ± 0.435
0.162MetMet: 0.162 ± 0.122
0.812MetAsn: 0.812 ± 0.308
1.462MetPro: 1.462 ± 0.317
0.731MetGln: 0.731 ± 0.265
1.543MetArg: 1.543 ± 0.455
2.762MetSer: 2.762 ± 0.398
1.868MetThr: 1.868 ± 0.452
1.543MetVal: 1.543 ± 0.315
0.487MetTrp: 0.487 ± 0.179
0.65MetTyr: 0.65 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
3.981AsnAla: 3.981 ± 0.511
0.406AsnCys: 0.406 ± 0.295
1.868AsnAsp: 1.868 ± 0.33
2.762AsnGlu: 2.762 ± 0.508
1.137AsnPhe: 1.137 ± 0.28
4.63AsnGly: 4.63 ± 0.649
0.894AsnHis: 0.894 ± 0.363
2.275AsnIle: 2.275 ± 0.489
1.787AsnLys: 1.787 ± 0.324
3.331AsnLeu: 3.331 ± 0.538
0.65AsnMet: 0.65 ± 0.19
1.219AsnAsn: 1.219 ± 0.362
2.843AsnPro: 2.843 ± 0.398
1.3AsnGln: 1.3 ± 0.297
2.031AsnArg: 2.031 ± 0.424
1.381AsnSer: 1.381 ± 0.382
1.95AsnThr: 1.95 ± 0.379
3.493AsnVal: 3.493 ± 0.579
0.731AsnTrp: 0.731 ± 0.252
1.787AsnTyr: 1.787 ± 0.548
0.0AsnXaa: 0.0 ± 0.0
Pro
3.249ProAla: 3.249 ± 0.507
0.406ProCys: 0.406 ± 0.163
2.924ProAsp: 2.924 ± 0.438
3.737ProGlu: 3.737 ± 0.556
1.543ProPhe: 1.543 ± 0.367
2.6ProGly: 2.6 ± 0.434
1.056ProHis: 1.056 ± 0.219
1.462ProIle: 1.462 ± 0.356
2.843ProLys: 2.843 ± 0.486
2.6ProLeu: 2.6 ± 0.531
0.65ProMet: 0.65 ± 0.215
2.681ProAsn: 2.681 ± 0.379
0.894ProPro: 0.894 ± 0.295
1.625ProGln: 1.625 ± 0.379
1.95ProArg: 1.95 ± 0.421
1.706ProSer: 1.706 ± 0.419
2.275ProThr: 2.275 ± 0.479
3.656ProVal: 3.656 ± 0.559
0.65ProTrp: 0.65 ± 0.288
0.812ProTyr: 0.812 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
4.63GlnAla: 4.63 ± 1.13
0.162GlnCys: 0.162 ± 0.113
2.681GlnAsp: 2.681 ± 0.523
2.924GlnGlu: 2.924 ± 0.491
2.112GlnPhe: 2.112 ± 0.356
3.818GlnGly: 3.818 ± 0.569
0.569GlnHis: 0.569 ± 0.219
1.543GlnIle: 1.543 ± 0.239
2.762GlnLys: 2.762 ± 0.607
3.981GlnLeu: 3.981 ± 0.52
1.543GlnMet: 1.543 ± 0.465
1.625GlnAsn: 1.625 ± 0.325
1.3GlnPro: 1.3 ± 0.373
2.843GlnGln: 2.843 ± 1.063
2.275GlnArg: 2.275 ± 0.694
1.95GlnSer: 1.95 ± 0.466
1.219GlnThr: 1.219 ± 0.251
2.031GlnVal: 2.031 ± 0.372
0.812GlnTrp: 0.812 ± 0.284
1.3GlnTyr: 1.3 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
4.712ArgAla: 4.712 ± 0.516
0.894ArgCys: 0.894 ± 0.255
3.818ArgAsp: 3.818 ± 0.669
3.656ArgGlu: 3.656 ± 0.493
2.518ArgPhe: 2.518 ± 0.435
4.712ArgGly: 4.712 ± 0.529
0.731ArgHis: 0.731 ± 0.305
2.681ArgIle: 2.681 ± 0.35
3.087ArgLys: 3.087 ± 0.574
5.361ArgLeu: 5.361 ± 0.624
1.625ArgMet: 1.625 ± 0.302
3.899ArgAsn: 3.899 ± 0.682
2.112ArgPro: 2.112 ± 0.404
2.681ArgGln: 2.681 ± 0.631
3.737ArgArg: 3.737 ± 0.537
3.493ArgSer: 3.493 ± 0.6
3.087ArgThr: 3.087 ± 0.546
4.062ArgVal: 4.062 ± 0.589
0.731ArgTrp: 0.731 ± 0.264
1.625ArgTyr: 1.625 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
5.605SerAla: 5.605 ± 0.688
0.65SerCys: 0.65 ± 0.196
4.387SerAsp: 4.387 ± 0.545
3.006SerGlu: 3.006 ± 0.572
2.356SerPhe: 2.356 ± 0.424
5.361SerGly: 5.361 ± 0.893
1.543SerHis: 1.543 ± 0.406
2.681SerIle: 2.681 ± 0.473
2.924SerLys: 2.924 ± 0.461
3.574SerLeu: 3.574 ± 0.455
1.706SerMet: 1.706 ± 0.36
1.787SerAsn: 1.787 ± 0.398
2.681SerPro: 2.681 ± 0.567
1.381SerGln: 1.381 ± 0.316
2.356SerArg: 2.356 ± 0.409
3.331SerSer: 3.331 ± 0.617
2.843SerThr: 2.843 ± 0.566
4.143SerVal: 4.143 ± 0.664
0.65SerTrp: 0.65 ± 0.177
2.275SerTyr: 2.275 ± 0.547
0.0SerXaa: 0.0 ± 0.0
Thr
5.605ThrAla: 5.605 ± 0.831
0.406ThrCys: 0.406 ± 0.181
2.681ThrAsp: 2.681 ± 0.451
4.305ThrGlu: 4.305 ± 0.491
1.868ThrPhe: 1.868 ± 0.403
7.067ThrGly: 7.067 ± 0.985
0.812ThrHis: 0.812 ± 0.246
3.818ThrIle: 3.818 ± 0.522
3.493ThrLys: 3.493 ± 0.459
4.549ThrLeu: 4.549 ± 0.619
1.3ThrMet: 1.3 ± 0.309
1.219ThrAsn: 1.219 ± 0.269
2.681ThrPro: 2.681 ± 0.459
2.112ThrGln: 2.112 ± 0.447
2.437ThrArg: 2.437 ± 0.411
3.168ThrSer: 3.168 ± 0.496
2.518ThrThr: 2.518 ± 0.428
4.468ThrVal: 4.468 ± 0.721
0.406ThrTrp: 0.406 ± 0.188
1.219ThrTyr: 1.219 ± 0.308
0.0ThrXaa: 0.0 ± 0.0
Val
6.255ValAla: 6.255 ± 0.798
0.406ValCys: 0.406 ± 0.187
3.899ValAsp: 3.899 ± 0.406
5.199ValGlu: 5.199 ± 0.823
2.518ValPhe: 2.518 ± 0.453
4.305ValGly: 4.305 ± 0.529
2.031ValHis: 2.031 ± 0.5
2.843ValIle: 2.843 ± 0.467
4.549ValLys: 4.549 ± 0.63
5.93ValLeu: 5.93 ± 0.789
1.543ValMet: 1.543 ± 0.394
2.843ValAsn: 2.843 ± 0.48
2.681ValPro: 2.681 ± 0.467
2.681ValGln: 2.681 ± 0.433
4.63ValArg: 4.63 ± 0.622
4.062ValSer: 4.062 ± 0.609
4.955ValThr: 4.955 ± 0.636
5.118ValVal: 5.118 ± 0.929
0.812ValTrp: 0.812 ± 0.269
2.275ValTyr: 2.275 ± 0.594
0.0ValXaa: 0.0 ± 0.0
Trp
1.543TrpAla: 1.543 ± 0.297
0.081TrpCys: 0.081 ± 0.087
0.812TrpAsp: 0.812 ± 0.297
0.406TrpGlu: 0.406 ± 0.279
0.569TrpPhe: 0.569 ± 0.214
1.137TrpGly: 1.137 ± 0.233
0.406TrpHis: 0.406 ± 0.184
0.325TrpIle: 0.325 ± 0.131
0.65TrpLys: 0.65 ± 0.252
1.706TrpLeu: 1.706 ± 0.33
0.487TrpMet: 0.487 ± 0.187
0.406TrpAsn: 0.406 ± 0.154
0.406TrpPro: 0.406 ± 0.199
0.894TrpGln: 0.894 ± 0.289
0.569TrpArg: 0.569 ± 0.221
1.462TrpSer: 1.462 ± 0.422
0.894TrpThr: 0.894 ± 0.26
1.381TrpVal: 1.381 ± 0.509
0.244TrpTrp: 0.244 ± 0.136
0.244TrpTyr: 0.244 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.924TyrAla: 2.924 ± 0.489
0.325TyrCys: 0.325 ± 0.222
1.787TyrAsp: 1.787 ± 0.281
2.275TyrGlu: 2.275 ± 0.574
0.812TyrPhe: 0.812 ± 0.261
3.331TyrGly: 3.331 ± 0.552
0.244TyrHis: 0.244 ± 0.132
1.381TyrIle: 1.381 ± 0.275
1.462TyrLys: 1.462 ± 0.296
2.031TyrLeu: 2.031 ± 0.407
0.975TyrMet: 0.975 ± 0.262
0.975TyrAsn: 0.975 ± 0.308
1.137TyrPro: 1.137 ± 0.279
1.3TyrGln: 1.3 ± 0.317
2.356TyrArg: 2.356 ± 0.421
1.219TyrSer: 1.219 ± 0.312
1.706TyrThr: 1.706 ± 0.446
1.868TyrVal: 1.868 ± 0.454
0.569TyrTrp: 0.569 ± 0.21
0.325TyrTyr: 0.325 ± 0.153
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12311 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski