Peste-des-petits-ruminants virus (PPRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Morbillivirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088DB31|A0A088DB31_PPRV RNA-directed RNA polymerase L OS=Peste-des-petits-ruminants virus OX=31604 GN=L PE=3 SV=1
MM1 pKa = 8.09AEE3 pKa = 3.72EE4 pKa = 3.86LAYY7 pKa = 10.45HH8 pKa = 5.15VVEE11 pKa = 5.56GLACLKK17 pKa = 10.75SLFASPPDD25 pKa = 3.55LSPIRR30 pKa = 11.84DD31 pKa = 3.57TLEE34 pKa = 3.45NWRR37 pKa = 11.84EE38 pKa = 3.92GLDD41 pKa = 3.44PSHH44 pKa = 7.24RR45 pKa = 11.84GTPNPDD51 pKa = 2.85MSEE54 pKa = 4.13GNHH57 pKa = 5.2QNINQSCPPAIGSGEE72 pKa = 3.95IDD74 pKa = 4.41LSTEE78 pKa = 3.77GNLGYY83 pKa = 10.4RR84 pKa = 11.84EE85 pKa = 4.05INYY88 pKa = 10.22DD89 pKa = 3.47DD90 pKa = 4.63SEE92 pKa = 5.99AGLRR96 pKa = 11.84GVQVYY101 pKa = 10.29GSHH104 pKa = 6.47PQIQRR109 pKa = 11.84YY110 pKa = 8.01HH111 pKa = 6.35VYY113 pKa = 9.03SHH115 pKa = 5.94GRR117 pKa = 11.84EE118 pKa = 4.12EE119 pKa = 5.01IEE121 pKa = 4.12GLQDD125 pKa = 3.47ADD127 pKa = 3.87SLMGQADD134 pKa = 4.04PPLANTFSTGEE145 pKa = 4.36DD146 pKa = 3.65GSDD149 pKa = 4.25DD150 pKa = 4.16SDD152 pKa = 3.79VDD154 pKa = 4.63SGPDD158 pKa = 3.33DD159 pKa = 4.83PDD161 pKa = 2.97RR162 pKa = 11.84HH163 pKa = 5.92PLYY166 pKa = 10.85DD167 pKa = 3.86RR168 pKa = 11.84GSVADD173 pKa = 3.81NDD175 pKa = 4.37DD176 pKa = 3.67VKK178 pKa = 11.09STDD181 pKa = 3.4VEE183 pKa = 4.22KK184 pKa = 11.52LEE186 pKa = 4.64GDD188 pKa = 4.68DD189 pKa = 3.45IQEE192 pKa = 4.11VLNSQKK198 pKa = 10.73SKK200 pKa = 10.69RR201 pKa = 11.84GRR203 pKa = 11.84FQGGKK208 pKa = 5.38TLRR211 pKa = 11.84VPEE214 pKa = 4.42TPDD217 pKa = 3.13VKK219 pKa = 10.6HH220 pKa = 6.34PRR222 pKa = 11.84PSAQSIKK229 pKa = 10.61KK230 pKa = 8.28GTDD233 pKa = 3.15GNSVLSGTVTEE244 pKa = 4.96CSSISGATQAVPEE257 pKa = 4.42SRR259 pKa = 11.84CEE261 pKa = 3.9SSEE264 pKa = 3.64RR265 pKa = 11.84NAFVEE270 pKa = 4.56SVPKK274 pKa = 10.04SARR277 pKa = 11.84SAKK280 pKa = 9.38TIQEE284 pKa = 4.12LTQEE288 pKa = 4.41SGTIASPTQPP298 pKa = 3.38

Molecular weight: 32.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q83138|Q83138_PPRV Nucleocapsid OS=Peste-des-petits-ruminants virus OX=31604 GN=N PE=2 SV=1
MM1 pKa = 7.52SWRR4 pKa = 11.84DD5 pKa = 3.22WHH7 pKa = 6.44VSSPSLPLPRR17 pKa = 11.84IFPPSEE23 pKa = 3.79IPLKK27 pKa = 9.62TGEE30 pKa = 4.61RR31 pKa = 11.84GLTHH35 pKa = 6.27PTVEE39 pKa = 4.6PRR41 pKa = 11.84TLICPRR47 pKa = 11.84GTIRR51 pKa = 11.84ISTNHH56 pKa = 5.54AHH58 pKa = 6.07QPSDD62 pKa = 3.61QAKK65 pKa = 8.17STCLLKK71 pKa = 10.94VISDD75 pKa = 3.79IEE77 pKa = 4.05RR78 pKa = 11.84SITMTVRR85 pKa = 11.84LDD87 pKa = 3.55SEE89 pKa = 4.71EE90 pKa = 4.11FRR92 pKa = 11.84STDD95 pKa = 3.13PTLKK99 pKa = 10.63YY100 pKa = 10.55SVTMFIATGVKK111 pKa = 9.63RR112 pKa = 11.84LKK114 pKa = 10.34DD115 pKa = 3.58SRR117 pKa = 11.84MLTLSWVKK125 pKa = 10.28QIHH128 pKa = 6.26HH129 pKa = 7.6LLTPSVQEE137 pKa = 4.4RR138 pKa = 11.84MDD140 pKa = 3.68LTTAMWTLAQMIPTDD155 pKa = 3.84ILYY158 pKa = 7.44MTGDD162 pKa = 4.72LLPTMMTLSPQMSKK176 pKa = 9.83SS177 pKa = 3.3

Molecular weight: 20.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

5182

177

2183

647.8

72.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.635 ± 0.542

1.737 ± 0.27

5.886 ± 0.534

6.04 ± 0.434

3.126 ± 0.399

6.6 ± 0.446

2.431 ± 0.445

7.179 ± 0.371

5.172 ± 0.405

10.305 ± 0.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.307

4.033 ± 0.215

4.844 ± 0.557

3.126 ± 0.364

5.905 ± 0.415

8.645 ± 0.651

6.098 ± 0.464

6.426 ± 0.336

0.965 ± 0.186

3.609 ± 0.555
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski