Chinook salmon bafinivirus
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3J9S7|A0A0E3J9S7_9NIDO Putative nucleocapsid protein OS=Chinook salmon bafinivirus OX=1611837 PE=4 SV=1
MM1 pKa = 7.9 ANLLWLTLLITLGLVSAQINTTLPDD26 pKa = 4.03 CPIPTCTYY34 pKa = 8.94 TPPTTTPFDD43 pKa = 3.62 HH44 pKa = 7.0 TSLHH48 pKa = 6.6 SFFTHH53 pKa = 5.74 DD54 pKa = 2.97 TNYY57 pKa = 11.12 AFLLSLKK64 pKa = 10.54 YY65 pKa = 10.64 NGTFIPATMAMPANNLSQTYY85 pKa = 7.86 TLNYY89 pKa = 9.68 KK90 pKa = 10.18 NVFVNVPDD98 pKa = 3.93 SQLPPPQRR106 pKa = 11.84 SNTQLAVVATTASCLTATYY125 pKa = 8.89 TAIPGTIPALAATDD139 pKa = 3.9 MYY141 pKa = 10.74 CVKK144 pKa = 9.68 FTQSSNFVYY153 pKa = 10.37 LYY155 pKa = 8.22 DD156 pKa = 3.75 TTVNTPVGVLLKK168 pKa = 10.87 NPTAPVVTTILSTIMVNRR186 pKa = 11.84 PTTVSTSQLLSNIYY200 pKa = 10.41 SLMRR204 pKa = 11.84 TRR206 pKa = 11.84 SNVIQQQFTCTSCLFAIDD224 pKa = 4.13 ASTGRR229 pKa = 11.84 AATSPTTHH237 pKa = 7.46 LLNNWSPSFFEE248 pKa = 4.79 TKK250 pKa = 9.38 TAMLDD255 pKa = 3.5 HH256 pKa = 7.3 VSMNTIYY263 pKa = 9.17 QTARR267 pKa = 11.84 GVTRR271 pKa = 11.84 EE272 pKa = 4.0 TFTSYY277 pKa = 10.88 ILNAAKK283 pKa = 9.01 TAAYY287 pKa = 10.33 ARR289 pKa = 11.84 GSPAYY294 pKa = 10.28 YY295 pKa = 9.51 IFSFAAIARR304 pKa = 11.84 YY305 pKa = 7.88 HH306 pKa = 5.23 QAMVTPYY313 pKa = 10.13 NYY315 pKa = 10.49 NIGKK319 pKa = 9.26 LCEE322 pKa = 3.88 ATAACCLSNGIEE334 pKa = 4.26 HH335 pKa = 6.9 FDD337 pKa = 3.03 IYY339 pKa = 10.93 TWLFHH344 pKa = 6.74 YY345 pKa = 10.67 YY346 pKa = 10.8 GFMKK350 pKa = 10.43 SKK352 pKa = 10.5 VYY354 pKa = 10.99 SEE356 pKa = 4.61 TFTTNPVLTSVINLATSIKK375 pKa = 9.56 GKK377 pKa = 10.02 HH378 pKa = 5.83 SSYY381 pKa = 11.27 CDD383 pKa = 3.31 LTPYY387 pKa = 10.96 SSFTAYY393 pKa = 10.87 NLIMLQDD400 pKa = 3.48 VVEE403 pKa = 4.41 QYY405 pKa = 10.92 QGTRR409 pKa = 11.84 TTSTYY414 pKa = 11.58 ANNFLNLTDD423 pKa = 3.49 INGYY427 pKa = 9.87 LPYY430 pKa = 9.53 STHH433 pKa = 6.42 TPLPQNYY440 pKa = 7.98 QNYY443 pKa = 7.91 MPFATYY449 pKa = 11.08 LLATFYY455 pKa = 11.36 SSFTLQQKK463 pKa = 10.31 FDD465 pKa = 3.45 FHH467 pKa = 7.44 RR468 pKa = 11.84 FLQSVCYY475 pKa = 9.25 YY476 pKa = 10.67 DD477 pKa = 3.62 SGFISRR483 pKa = 11.84 NQAYY487 pKa = 10.13 HH488 pKa = 6.38 SICHH492 pKa = 6.88 IITLKK497 pKa = 10.11 TSSSVPYY504 pKa = 9.39 PAEE507 pKa = 4.03 LQWPFTALPSSGYY520 pKa = 10.59 DD521 pKa = 3.17 SYY523 pKa = 11.57 PVSTGLGTVAVGNTNPLDD541 pKa = 3.83 YY542 pKa = 10.86 PGICYY547 pKa = 10.15 QSVCLTHH554 pKa = 5.66 PTINFRR560 pKa = 11.84 LLLSEE565 pKa = 5.98 LPIKK569 pKa = 10.36 WEE571 pKa = 3.69 VRR573 pKa = 11.84 PEE575 pKa = 4.42 GIYY578 pKa = 9.03 VTSRR582 pKa = 11.84 NDD584 pKa = 3.38 PTCLNFKK591 pKa = 9.41 TSQLGTVFITLYY603 pKa = 10.72 DD604 pKa = 3.89 SNAXCTLQQTTYY616 pKa = 10.73 SRR618 pKa = 11.84 SAVLRR623 pKa = 11.84 QVNTFTPNATDD634 pKa = 3.54 LVVPYY639 pKa = 10.41 GKK641 pKa = 8.51 TPIYY645 pKa = 10.52 NGAYY649 pKa = 9.78 NINSTASGAVSXSTNTHH666 pKa = 6.31 YY667 pKa = 10.48 IPRR670 pKa = 11.84 LLTSVLTPQSDD681 pKa = 4.12 YY682 pKa = 11.2 KK683 pKa = 10.13 ITPTTTNLEE692 pKa = 4.11 PFVEE696 pKa = 4.47 INGTTFYY703 pKa = 11.39 YY704 pKa = 10.47 DD705 pKa = 3.2 ANQAYY710 pKa = 10.47 VNTSDD715 pKa = 4.41 VYY717 pKa = 10.42 QANVDD722 pKa = 4.0 FGLPQTFSLDD732 pKa = 3.52 TSFLNCTAFLCASDD746 pKa = 4.14 YY747 pKa = 11.45 KK748 pKa = 10.89 CIADD752 pKa = 4.3 FSPICSATQPLIDD765 pKa = 5.38 ALLQTYY771 pKa = 7.33 TLYY774 pKa = 10.82 NQQLTLYY781 pKa = 9.19 NSSLLTIQAAANYY794 pKa = 10.06 LYY796 pKa = 8.9 DD797 pKa = 3.09 TRR799 pKa = 11.84 TRR801 pKa = 11.84 TRR803 pKa = 11.84 RR804 pKa = 11.84 SLLSTVNLGYY814 pKa = 11.06 GDD816 pKa = 4.95 VFQQPAWVYY825 pKa = 10.45 EE826 pKa = 4.14 IGATAAIPMIGPAVSVGLLASRR848 pKa = 11.84 VASIEE853 pKa = 3.93 STLYY857 pKa = 10.83 QITQGVKK864 pKa = 9.38 EE865 pKa = 5.15 SIMAMNTKK873 pKa = 9.88 LDD875 pKa = 3.98 KK876 pKa = 10.51 IANILHH882 pKa = 6.39 SDD884 pKa = 3.74 IEE886 pKa = 4.39 RR887 pKa = 11.84 LGLTVNTLASSFNTFTKK904 pKa = 10.55 QVTDD908 pKa = 3.39 KK909 pKa = 10.64 FASVDD914 pKa = 3.45 VAFNDD919 pKa = 4.54 LSTSLNTLTTTTTVGASYY937 pKa = 10.35 AAQINFATTTLTNLEE952 pKa = 4.21 VQISQTTDD960 pKa = 2.68 HH961 pKa = 6.98 ALSCIAALNEE971 pKa = 4.4 HH972 pKa = 6.37 VLSPHH977 pKa = 6.93 CISVSQLSAYY987 pKa = 8.28 TPNALSNIGEE997 pKa = 4.39 KK998 pKa = 10.54 LVLTHH1003 pKa = 6.64 YY1004 pKa = 10.85 INGSLITFYY1013 pKa = 11.11 HH1014 pKa = 6.95 LIASKK1019 pKa = 10.58 GVYY1022 pKa = 9.56 RR1023 pKa = 11.84 PLPKK1027 pKa = 9.67 MYY1029 pKa = 10.51 NSTHH1033 pKa = 6.38 HH1034 pKa = 6.71 LVATTGFYY1042 pKa = 10.47 NGHH1045 pKa = 6.93 CIFCGEE1051 pKa = 4.56 HH1052 pKa = 5.81 YY1053 pKa = 10.62 CDD1055 pKa = 3.65 ISVSVPCDD1063 pKa = 3.23 YY1064 pKa = 10.91 AYY1066 pKa = 10.25 EE1067 pKa = 4.2 PLKK1070 pKa = 10.55 STVLAAYY1077 pKa = 8.35 PLTTGVALLTLDD1089 pKa = 3.47 STATNFIINYY1099 pKa = 9.02 NNAPLISSSVPQPPIINNTDD1119 pKa = 3.09 GTVITLQQQIIQLQTQFDD1137 pKa = 4.07 HH1138 pKa = 7.17 FNISSNFHH1146 pKa = 5.78 VSEE1149 pKa = 3.92 IQPIIDD1155 pKa = 3.87 YY1156 pKa = 11.06 YY1157 pKa = 10.58 NAQINSSLNQIVDD1170 pKa = 4.26 FGNGSTSNVGTIILIVLVVALALAAAIAIICGLKK1204 pKa = 10.12 HH1205 pKa = 5.93
Molecular weight: 132.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.752
IPC2_protein 5.779
IPC_protein 5.868
Toseland 6.224
ProMoST 6.237
Dawson 6.211
Bjellqvist 6.198
Wikipedia 6.237
Rodwell 6.198
Grimsley 6.351
Solomon 6.211
Lehninger 6.198
Nozaki 6.504
DTASelect 6.678
Thurlkill 6.707
EMBOSS 6.664
Sillero 6.59
Patrickios 0.223
IPC_peptide 6.224
IPC2_peptide 6.576
IPC2.peptide.svr19 6.464
Protein with the highest isoelectric point:
>tr|A0A0E3J9X8|A0A0E3J9X8_9NIDO Membrane protein OS=Chinook salmon bafinivirus OX=1611837 PE=4 SV=1
MM1 pKa = 7.56 AFYY4 pKa = 9.94 GPPMMPMSMPASFPPRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 APKK25 pKa = 9.86 KK26 pKa = 10.13 RR27 pKa = 11.84 PQQKK31 pKa = 9.51 KK32 pKa = 7.16 QPKK35 pKa = 8.17 PQPKK39 pKa = 9.39 PLNDD43 pKa = 3.56 QQKK46 pKa = 7.53 TAVAKK51 pKa = 9.94 EE52 pKa = 4.32 AIAKK56 pKa = 7.53 ATTVPMPPMAPVVKK70 pKa = 9.83 TSLRR74 pKa = 11.84 TQVIKK79 pKa = 10.84 GKK81 pKa = 10.44 RR82 pKa = 11.84 GEE84 pKa = 4.1 LQDD87 pKa = 3.2 ARR89 pKa = 11.84 GRR91 pKa = 11.84 LTQSEE96 pKa = 4.26 INQLYY101 pKa = 9.01 FQIATALNSGLGEE114 pKa = 4.04 IGIHH118 pKa = 6.04 NAATQEE124 pKa = 4.05 GVRR127 pKa = 11.84 LIVRR131 pKa = 11.84 LNLAPPNSIAEE142 pKa = 4.29 KK143 pKa = 10.02 LYY145 pKa = 10.31 KK146 pKa = 10.24 SHH148 pKa = 7.25 AAAHH152 pKa = 6.14 LNSEE156 pKa = 4.18 ALEE159 pKa = 4.43 SKK161 pKa = 10.49 FKK163 pKa = 10.71 EE164 pKa = 4.26 RR165 pKa = 11.84 EE166 pKa = 3.9 SDD168 pKa = 3.67 PLFHH172 pKa = 7.78 FSTTPEE178 pKa = 3.71
Molecular weight: 19.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.141
IPC2_protein 9.502
IPC_protein 9.545
Toseland 10.555
ProMoST 10.043
Dawson 10.628
Bjellqvist 10.218
Wikipedia 10.745
Rodwell 11.213
Grimsley 10.657
Solomon 10.672
Lehninger 10.657
Nozaki 10.511
DTASelect 10.218
Thurlkill 10.526
EMBOSS 10.921
Sillero 10.54
Patrickios 10.95
IPC_peptide 10.687
IPC2_peptide 8.58
IPC2.peptide.svr19 8.667
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
10919
178
7011
2183.8
244.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.896 ± 0.283
1.951 ± 0.18
4.515 ± 0.361
3.627 ± 0.419
4.506 ± 0.169
4.277 ± 0.197
3.7 ± 0.377
6.768 ± 0.155
4.671 ± 0.524
9.387 ± 0.403
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.914 ± 0.225
5.696 ± 0.277
5.761 ± 0.254
4.185 ± 0.153
2.931 ± 0.217
7.217 ± 0.394
10.505 ± 0.702
5.843 ± 0.16
0.632 ± 0.035
4.991 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here