Simian torque teno virus 32
Average proteome isoelectric point is 8.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5IMV7|A0A0C5IMV7_9VIRU ORF2 OS=Simian torque teno virus 32 OX=1619220 PE=4 SV=1
MM1 pKa = 7.24 FSFRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 VHH11 pKa = 4.89 GQTRR15 pKa = 11.84 QRR17 pKa = 11.84 PSLLSMFWKK26 pKa = 10.5 KK27 pKa = 10.21 SAEE30 pKa = 3.98 DD31 pKa = 3.11 AGAWEE36 pKa = 4.35 EE37 pKa = 3.72 RR38 pKa = 11.84 WLRR41 pKa = 11.84 SVYY44 pKa = 9.01 DD45 pKa = 3.42 THH47 pKa = 9.47 ALFCSCNYY55 pKa = 10.19 FLGHH59 pKa = 7.1 LSAIQARR66 pKa = 11.84 HH67 pKa = 6.01 RR68 pKa = 11.84 GPRR71 pKa = 11.84 GQSRR75 pKa = 11.84 TPPTLDD81 pKa = 3.01 GLGPPSPPRR90 pKa = 11.84 LAAPPPTPASSRR102 pKa = 11.84 ASTTPISLLHH112 pKa = 6.37 SGGSSSSSGVHH123 pKa = 6.13 TDD125 pKa = 3.82 SPWSTGGDD133 pKa = 3.34 GDD135 pKa = 4.09 AGGPGGDD142 pKa = 3.64 GAGQDD147 pKa = 3.64 GVDD150 pKa = 3.73 AFTAEE155 pKa = 3.99 QLEE158 pKa = 4.32 EE159 pKa = 3.91 LFAAVEE165 pKa = 3.87 EE166 pKa = 4.53 DD167 pKa = 3.49 ARR169 pKa = 4.84
Molecular weight: 18.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.697
IPC2_protein 6.491
IPC_protein 6.868
Toseland 6.971
ProMoST 7.439
Dawson 7.307
Bjellqvist 7.015
Wikipedia 7.278
Rodwell 7.263
Grimsley 7.073
Solomon 7.41
Lehninger 7.41
Nozaki 7.395
DTASelect 7.556
Thurlkill 7.585
EMBOSS 7.673
Sillero 7.658
Patrickios 4.774
IPC_peptide 7.41
IPC2_peptide 7.41
IPC2.peptide.svr19 7.185
Protein with the highest isoelectric point:
>tr|A0A0C5IBK5|A0A0C5IBK5_9VIRU Capsid protein OS=Simian torque teno virus 32 OX=1619220 PE=3 SV=1
MM1 pKa = 7.62 EE2 pKa = 5.44 DD3 pKa = 3.52 ARR5 pKa = 11.84 RR6 pKa = 11.84 ASPPLCKK13 pKa = 10.02 KK14 pKa = 10.25 KK15 pKa = 10.74 LVFLGHH21 pKa = 6.7 GRR23 pKa = 11.84 GQPPAGVATEE33 pKa = 4.56 KK34 pKa = 9.75 KK35 pKa = 7.74 TQNAEE40 pKa = 3.78 QRR42 pKa = 11.84 GGPGVPGPPPAARR55 pKa = 11.84 AAAGAVPPRR64 pKa = 11.84 PTPATATAPKK74 pKa = 8.61 KK75 pKa = 8.52 TPKK78 pKa = 10.15 SPRR81 pKa = 11.84 RR82 pKa = 11.84 DD83 pKa = 3.29 LAEE86 pKa = 4.55 AGPNRR91 pKa = 11.84 GPPTARR97 pKa = 11.84 PPAIVIHH104 pKa = 6.57 RR105 pKa = 11.84 GSHH108 pKa = 4.28 TVSMFHH114 pKa = 7.12 EE115 pKa = 4.85 NPQVSYY121 pKa = 11.46 NNQRR125 pKa = 11.84 EE126 pKa = 4.33 YY127 pKa = 11.49 NQFEE131 pKa = 4.96 LDD133 pKa = 3.67 TEE135 pKa = 4.43 RR136 pKa = 11.84 EE137 pKa = 3.85 LAQAFMRR144 pKa = 11.84 YY145 pKa = 8.87 PRR147 pKa = 11.84 IRR149 pKa = 11.84 PRR151 pKa = 11.84 PYY153 pKa = 9.72 EE154 pKa = 3.8 VPYY157 pKa = 10.04 YY158 pKa = 8.28 PWVYY162 pKa = 10.21 HH163 pKa = 5.58 SVSTRR168 pKa = 11.84 TEE170 pKa = 3.92 FPKK173 pKa = 10.77 VSFALRR179 pKa = 11.84 FNPFSINN186 pKa = 3.09
Molecular weight: 20.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.765
IPC_protein 10.555
Toseland 10.555
ProMoST 10.277
Dawson 10.701
Bjellqvist 10.409
Wikipedia 10.921
Rodwell 10.921
Grimsley 10.76
Solomon 10.789
Lehninger 10.745
Nozaki 10.54
DTASelect 10.409
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.613
Patrickios 10.613
IPC_peptide 10.789
IPC2_peptide 9.311
IPC2.peptide.svr19 8.569
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1265
148
762
316.3
36.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.929 ± 2.112
1.344 ± 0.306
3.399 ± 0.675
4.822 ± 0.318
3.241 ± 0.551
7.115 ± 1.016
2.53 ± 0.378
2.925 ± 0.764
4.664 ± 0.76
7.352 ± 1.425
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.66 ± 0.157
3.794 ± 0.806
8.933 ± 1.85
5.138 ± 0.582
11.067 ± 0.677
8.458 ± 3.089
6.877 ± 0.389
4.032 ± 0.801
2.925 ± 0.998
3.794 ± 1.084
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here