Amino acid dipepetide frequency for Mycobacterium phage Serenity

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.999AlaAla: 9.999 ± 0.947
0.683AlaCys: 0.683 ± 0.215
5.651AlaAsp: 5.651 ± 0.602
8.011AlaGlu: 8.011 ± 0.756
3.664AlaPhe: 3.664 ± 0.45
8.57AlaGly: 8.57 ± 1.037
1.863AlaHis: 1.863 ± 0.355
4.782AlaIle: 4.782 ± 0.472
5.093AlaLys: 5.093 ± 0.532
8.881AlaLeu: 8.881 ± 0.9
2.857AlaMet: 2.857 ± 0.428
3.043AlaAsn: 3.043 ± 0.525
4.844AlaPro: 4.844 ± 0.638
3.975AlaGln: 3.975 ± 0.617
5.341AlaArg: 5.341 ± 0.642
4.534AlaSer: 4.534 ± 0.478
4.906AlaThr: 4.906 ± 0.606
6.645AlaVal: 6.645 ± 0.68
1.987AlaTrp: 1.987 ± 0.407
2.981AlaTyr: 2.981 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.559CysAla: 0.559 ± 0.164
0.0CysCys: 0.0 ± 0.0
0.807CysAsp: 0.807 ± 0.211
0.683CysGlu: 0.683 ± 0.23
0.435CysPhe: 0.435 ± 0.159
0.559CysGly: 0.559 ± 0.193
0.186CysHis: 0.186 ± 0.09
0.248CysIle: 0.248 ± 0.132
0.497CysLys: 0.497 ± 0.147
0.683CysLeu: 0.683 ± 0.189
0.186CysMet: 0.186 ± 0.129
0.311CysAsn: 0.311 ± 0.123
0.435CysPro: 0.435 ± 0.171
0.186CysGln: 0.186 ± 0.117
0.807CysArg: 0.807 ± 0.225
0.311CysSer: 0.311 ± 0.149
0.559CysThr: 0.559 ± 0.169
0.559CysVal: 0.559 ± 0.181
0.435CysTrp: 0.435 ± 0.175
0.248CysTyr: 0.248 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
5.714AspAla: 5.714 ± 0.585
0.559AspCys: 0.559 ± 0.211
3.788AspAsp: 3.788 ± 0.487
5.093AspGlu: 5.093 ± 0.652
2.422AspPhe: 2.422 ± 0.399
5.403AspGly: 5.403 ± 0.674
1.366AspHis: 1.366 ± 0.326
3.416AspIle: 3.416 ± 0.441
2.608AspLys: 2.608 ± 0.382
5.217AspLeu: 5.217 ± 0.658
1.615AspMet: 1.615 ± 0.25
1.739AspAsn: 1.739 ± 0.351
4.72AspPro: 4.72 ± 0.609
2.298AspGln: 2.298 ± 0.417
3.167AspArg: 3.167 ± 0.509
2.857AspSer: 2.857 ± 0.437
3.292AspThr: 3.292 ± 0.494
4.347AspVal: 4.347 ± 0.473
1.304AspTrp: 1.304 ± 0.342
2.298AspTyr: 2.298 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
6.956GluAla: 6.956 ± 0.817
0.311GluCys: 0.311 ± 0.119
4.534GluAsp: 4.534 ± 0.601
4.906GluGlu: 4.906 ± 0.686
3.416GluPhe: 3.416 ± 0.484
5.403GluGly: 5.403 ± 0.577
1.553GluHis: 1.553 ± 0.341
4.347GluIle: 4.347 ± 0.513
2.546GluLys: 2.546 ± 0.429
7.887GluLeu: 7.887 ± 0.708
2.484GluMet: 2.484 ± 0.426
2.546GluAsn: 2.546 ± 0.381
2.608GluPro: 2.608 ± 0.461
1.553GluGln: 1.553 ± 0.255
5.03GluArg: 5.03 ± 0.609
2.484GluSer: 2.484 ± 0.416
3.726GluThr: 3.726 ± 0.466
4.658GluVal: 4.658 ± 0.634
1.366GluTrp: 1.366 ± 0.284
2.484GluTyr: 2.484 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
3.167PheAla: 3.167 ± 0.494
0.248PheCys: 0.248 ± 0.138
2.795PheAsp: 2.795 ± 0.555
2.67PheGlu: 2.67 ± 0.319
0.745PhePhe: 0.745 ± 0.175
3.105PheGly: 3.105 ± 0.433
0.683PheHis: 0.683 ± 0.255
1.553PheIle: 1.553 ± 0.327
1.49PheLys: 1.49 ± 0.284
2.112PheLeu: 2.112 ± 0.408
0.435PheMet: 0.435 ± 0.169
1.615PheAsn: 1.615 ± 0.257
1.863PhePro: 1.863 ± 0.413
1.553PheGln: 1.553 ± 0.395
2.733PheArg: 2.733 ± 0.435
1.925PheSer: 1.925 ± 0.313
2.484PheThr: 2.484 ± 0.305
2.298PheVal: 2.298 ± 0.364
0.683PheTrp: 0.683 ± 0.217
1.056PheTyr: 1.056 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
7.515GlyAla: 7.515 ± 1.082
0.745GlyCys: 0.745 ± 0.24
5.962GlyAsp: 5.962 ± 0.761
4.347GlyGlu: 4.347 ± 0.478
3.105GlyPhe: 3.105 ± 0.447
9.067GlyGly: 9.067 ± 1.438
1.553GlyHis: 1.553 ± 0.303
4.285GlyIle: 4.285 ± 0.634
4.72GlyLys: 4.72 ± 0.487
6.086GlyLeu: 6.086 ± 0.752
2.112GlyMet: 2.112 ± 0.574
2.733GlyAsn: 2.733 ± 0.453
3.292GlyPro: 3.292 ± 0.369
2.733GlyGln: 2.733 ± 0.473
4.285GlyArg: 4.285 ± 0.543
3.726GlySer: 3.726 ± 0.559
5.03GlyThr: 5.03 ± 0.64
6.769GlyVal: 6.769 ± 0.762
1.677GlyTrp: 1.677 ± 0.331
2.298GlyTyr: 2.298 ± 0.306
0.0GlyXaa: 0.0 ± 0.0
His
1.739HisAla: 1.739 ± 0.333
0.497HisCys: 0.497 ± 0.158
1.863HisAsp: 1.863 ± 0.386
1.428HisGlu: 1.428 ± 0.385
0.497HisPhe: 0.497 ± 0.146
1.428HisGly: 1.428 ± 0.358
0.807HisHis: 0.807 ± 0.259
1.49HisIle: 1.49 ± 0.285
1.118HisLys: 1.118 ± 0.284
1.615HisLeu: 1.615 ± 0.285
0.248HisMet: 0.248 ± 0.117
0.559HisAsn: 0.559 ± 0.194
1.366HisPro: 1.366 ± 0.301
0.807HisGln: 0.807 ± 0.235
1.987HisArg: 1.987 ± 0.379
0.869HisSer: 0.869 ± 0.23
1.118HisThr: 1.118 ± 0.258
0.994HisVal: 0.994 ± 0.245
0.311HisTrp: 0.311 ± 0.149
0.807HisTyr: 0.807 ± 0.318
0.0HisXaa: 0.0 ± 0.0
Ile
5.527IleAla: 5.527 ± 0.559
0.435IleCys: 0.435 ± 0.152
3.54IleAsp: 3.54 ± 0.459
4.844IleGlu: 4.844 ± 0.633
1.304IlePhe: 1.304 ± 0.28
3.664IleGly: 3.664 ± 0.543
1.366IleHis: 1.366 ± 0.278
1.615IleIle: 1.615 ± 0.291
2.236IleLys: 2.236 ± 0.356
4.037IleLeu: 4.037 ± 0.371
0.559IleMet: 0.559 ± 0.17
1.801IleAsn: 1.801 ± 0.319
3.788IlePro: 3.788 ± 0.44
1.553IleGln: 1.553 ± 0.317
3.292IleArg: 3.292 ± 0.476
2.733IleSer: 2.733 ± 0.5
3.043IleThr: 3.043 ± 0.374
3.229IleVal: 3.229 ± 0.382
0.559IleTrp: 0.559 ± 0.152
1.056IleTyr: 1.056 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
5.962LysAla: 5.962 ± 0.722
0.186LysCys: 0.186 ± 0.118
2.733LysAsp: 2.733 ± 0.417
2.112LysGlu: 2.112 ± 0.355
1.118LysPhe: 1.118 ± 0.249
3.167LysGly: 3.167 ± 0.434
0.994LysHis: 0.994 ± 0.281
2.112LysIle: 2.112 ± 0.306
3.167LysLys: 3.167 ± 0.62
3.478LysLeu: 3.478 ± 0.477
1.18LysMet: 1.18 ± 0.243
1.304LysAsn: 1.304 ± 0.283
3.726LysPro: 3.726 ± 0.641
1.49LysGln: 1.49 ± 0.313
3.54LysArg: 3.54 ± 0.541
2.484LysSer: 2.484 ± 0.345
2.67LysThr: 2.67 ± 0.449
4.534LysVal: 4.534 ± 0.493
0.869LysTrp: 0.869 ± 0.228
1.304LysTyr: 1.304 ± 0.33
0.0LysXaa: 0.0 ± 0.0
Leu
9.067LeuAla: 9.067 ± 0.855
0.621LeuCys: 0.621 ± 0.202
4.534LeuAsp: 4.534 ± 0.465
5.9LeuGlu: 5.9 ± 0.549
2.795LeuPhe: 2.795 ± 0.38
6.769LeuGly: 6.769 ± 1.159
1.987LeuHis: 1.987 ± 0.389
4.72LeuIle: 4.72 ± 0.582
3.602LeuLys: 3.602 ± 0.381
5.155LeuLeu: 5.155 ± 0.565
2.608LeuMet: 2.608 ± 0.409
2.795LeuAsn: 2.795 ± 0.403
4.037LeuPro: 4.037 ± 0.506
2.608LeuGln: 2.608 ± 0.671
5.279LeuArg: 5.279 ± 0.717
4.844LeuSer: 4.844 ± 0.635
4.223LeuThr: 4.223 ± 0.665
4.844LeuVal: 4.844 ± 0.618
1.615LeuTrp: 1.615 ± 0.236
2.236LeuTyr: 2.236 ± 0.416
0.0LeuXaa: 0.0 ± 0.0
Met
2.919MetAla: 2.919 ± 0.422
0.186MetCys: 0.186 ± 0.112
1.118MetAsp: 1.118 ± 0.252
1.553MetGlu: 1.553 ± 0.337
0.373MetPhe: 0.373 ± 0.151
1.677MetGly: 1.677 ± 0.379
0.373MetHis: 0.373 ± 0.135
1.366MetIle: 1.366 ± 0.253
1.987MetLys: 1.987 ± 0.334
1.677MetLeu: 1.677 ± 0.4
0.683MetMet: 0.683 ± 0.228
0.745MetAsn: 0.745 ± 0.228
1.553MetPro: 1.553 ± 0.374
1.18MetGln: 1.18 ± 0.337
1.553MetArg: 1.553 ± 0.305
2.795MetSer: 2.795 ± 0.37
2.36MetThr: 2.36 ± 0.355
1.242MetVal: 1.242 ± 0.277
0.186MetTrp: 0.186 ± 0.1
0.621MetTyr: 0.621 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
2.67AsnAla: 2.67 ± 0.49
0.373AsnCys: 0.373 ± 0.137
1.304AsnAsp: 1.304 ± 0.263
2.422AsnGlu: 2.422 ± 0.39
0.994AsnPhe: 0.994 ± 0.254
3.602AsnGly: 3.602 ± 0.403
0.745AsnHis: 0.745 ± 0.227
1.118AsnIle: 1.118 ± 0.298
0.745AsnLys: 0.745 ± 0.18
2.733AsnLeu: 2.733 ± 0.361
0.994AsnMet: 0.994 ± 0.231
0.807AsnAsn: 0.807 ± 0.203
2.484AsnPro: 2.484 ± 0.379
1.056AsnGln: 1.056 ± 0.274
2.67AsnArg: 2.67 ± 0.468
1.304AsnSer: 1.304 ± 0.235
2.236AsnThr: 2.236 ± 0.512
2.484AsnVal: 2.484 ± 0.372
0.621AsnTrp: 0.621 ± 0.182
1.056AsnTyr: 1.056 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
4.72ProAla: 4.72 ± 0.609
0.435ProCys: 0.435 ± 0.167
3.416ProAsp: 3.416 ± 0.411
4.347ProGlu: 4.347 ± 0.572
1.987ProPhe: 1.987 ± 0.394
4.471ProGly: 4.471 ± 0.52
1.118ProHis: 1.118 ± 0.219
3.292ProIle: 3.292 ± 0.346
2.484ProLys: 2.484 ± 0.61
3.354ProLeu: 3.354 ± 0.43
1.242ProMet: 1.242 ± 0.313
2.112ProAsn: 2.112 ± 0.327
1.863ProPro: 1.863 ± 0.439
1.987ProGln: 1.987 ± 0.393
3.416ProArg: 3.416 ± 0.541
2.608ProSer: 2.608 ± 0.357
3.85ProThr: 3.85 ± 0.507
3.788ProVal: 3.788 ± 0.438
1.118ProTrp: 1.118 ± 0.379
1.49ProTyr: 1.49 ± 0.308
0.0ProXaa: 0.0 ± 0.0
Gln
3.788GlnAla: 3.788 ± 0.627
0.311GlnCys: 0.311 ± 0.118
1.242GlnAsp: 1.242 ± 0.33
1.863GlnGlu: 1.863 ± 0.341
1.615GlnPhe: 1.615 ± 0.32
2.981GlnGly: 2.981 ± 0.441
1.056GlnHis: 1.056 ± 0.253
2.112GlnIle: 2.112 ± 0.368
1.987GlnLys: 1.987 ± 0.338
3.664GlnLeu: 3.664 ± 0.619
1.118GlnMet: 1.118 ± 0.332
1.056GlnAsn: 1.056 ± 0.288
1.18GlnPro: 1.18 ± 0.274
2.049GlnGln: 2.049 ± 0.546
2.422GlnArg: 2.422 ± 0.385
1.615GlnSer: 1.615 ± 0.329
1.925GlnThr: 1.925 ± 0.367
2.298GlnVal: 2.298 ± 0.368
0.932GlnTrp: 0.932 ± 0.246
0.745GlnTyr: 0.745 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
5.651ArgAla: 5.651 ± 0.628
1.18ArgCys: 1.18 ± 0.25
4.347ArgAsp: 4.347 ± 0.57
5.776ArgGlu: 5.776 ± 0.71
2.484ArgPhe: 2.484 ± 0.572
3.788ArgGly: 3.788 ± 0.555
0.869ArgHis: 0.869 ± 0.259
4.161ArgIle: 4.161 ± 0.546
3.478ArgLys: 3.478 ± 0.437
5.217ArgLeu: 5.217 ± 0.487
2.36ArgMet: 2.36 ± 0.324
2.36ArgAsn: 2.36 ± 0.37
2.608ArgPro: 2.608 ± 0.36
1.987ArgGln: 1.987 ± 0.284
5.279ArgArg: 5.279 ± 0.582
3.043ArgSer: 3.043 ± 0.551
2.36ArgThr: 2.36 ± 0.295
5.217ArgVal: 5.217 ± 0.509
1.428ArgTrp: 1.428 ± 0.29
2.236ArgTyr: 2.236 ± 0.434
0.0ArgXaa: 0.0 ± 0.0
Ser
4.782SerAla: 4.782 ± 0.486
0.373SerCys: 0.373 ± 0.175
3.416SerAsp: 3.416 ± 0.386
3.602SerGlu: 3.602 ± 0.61
2.112SerPhe: 2.112 ± 0.4
4.471SerGly: 4.471 ± 0.609
1.118SerHis: 1.118 ± 0.279
1.925SerIle: 1.925 ± 0.325
2.049SerLys: 2.049 ± 0.443
4.223SerLeu: 4.223 ± 0.642
1.118SerMet: 1.118 ± 0.238
1.118SerAsn: 1.118 ± 0.302
3.167SerPro: 3.167 ± 0.366
1.863SerGln: 1.863 ± 0.328
3.975SerArg: 3.975 ± 0.519
2.67SerSer: 2.67 ± 0.621
2.67SerThr: 2.67 ± 0.347
3.54SerVal: 3.54 ± 0.51
1.428SerTrp: 1.428 ± 0.333
0.807SerTyr: 0.807 ± 0.191
0.0SerXaa: 0.0 ± 0.0
Thr
6.397ThrAla: 6.397 ± 0.745
0.497ThrCys: 0.497 ± 0.173
3.726ThrAsp: 3.726 ± 0.479
2.795ThrGlu: 2.795 ± 0.408
2.546ThrPhe: 2.546 ± 0.466
4.968ThrGly: 4.968 ± 0.63
1.118ThrHis: 1.118 ± 0.238
2.422ThrIle: 2.422 ± 0.46
3.229ThrLys: 3.229 ± 0.502
4.409ThrLeu: 4.409 ± 0.617
1.615ThrMet: 1.615 ± 0.314
1.801ThrAsn: 1.801 ± 0.327
3.85ThrPro: 3.85 ± 0.488
2.236ThrGln: 2.236 ± 0.352
3.229ThrArg: 3.229 ± 0.415
2.857ThrSer: 2.857 ± 0.432
3.292ThrThr: 3.292 ± 0.487
4.72ThrVal: 4.72 ± 0.737
0.994ThrTrp: 0.994 ± 0.225
1.739ThrTyr: 1.739 ± 0.301
0.0ThrXaa: 0.0 ± 0.0
Val
6.956ValAla: 6.956 ± 0.844
0.683ValCys: 0.683 ± 0.203
5.155ValAsp: 5.155 ± 0.61
5.03ValGlu: 5.03 ± 0.481
2.236ValPhe: 2.236 ± 0.511
5.279ValGly: 5.279 ± 0.692
1.615ValHis: 1.615 ± 0.312
2.919ValIle: 2.919 ± 0.551
3.416ValLys: 3.416 ± 0.401
5.651ValLeu: 5.651 ± 0.6
1.304ValMet: 1.304 ± 0.297
2.608ValAsn: 2.608 ± 0.398
3.354ValPro: 3.354 ± 0.559
2.422ValGln: 2.422 ± 0.352
4.471ValArg: 4.471 ± 0.558
3.85ValSer: 3.85 ± 0.519
4.968ValThr: 4.968 ± 0.622
5.155ValVal: 5.155 ± 0.538
1.366ValTrp: 1.366 ± 0.315
2.174ValTyr: 2.174 ± 0.368
0.0ValXaa: 0.0 ± 0.0
Trp
1.863TrpAla: 1.863 ± 0.411
0.248TrpCys: 0.248 ± 0.135
1.304TrpAsp: 1.304 ± 0.289
1.366TrpGlu: 1.366 ± 0.253
0.683TrpPhe: 0.683 ± 0.197
1.49TrpGly: 1.49 ± 0.342
0.932TrpHis: 0.932 ± 0.273
0.932TrpIle: 0.932 ± 0.232
0.621TrpLys: 0.621 ± 0.224
1.118TrpLeu: 1.118 ± 0.332
0.435TrpMet: 0.435 ± 0.146
0.497TrpAsn: 0.497 ± 0.195
0.869TrpPro: 0.869 ± 0.266
1.18TrpGln: 1.18 ± 0.294
1.056TrpArg: 1.056 ± 0.258
1.18TrpSer: 1.18 ± 0.276
1.615TrpThr: 1.615 ± 0.361
1.18TrpVal: 1.18 ± 0.261
0.497TrpTrp: 0.497 ± 0.178
0.621TrpTyr: 0.621 ± 0.232
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.36TyrAla: 2.36 ± 0.407
0.124TyrCys: 0.124 ± 0.085
1.987TyrAsp: 1.987 ± 0.327
2.049TyrGlu: 2.049 ± 0.363
0.683TyrPhe: 0.683 ± 0.194
2.236TyrGly: 2.236 ± 0.379
0.248TyrHis: 0.248 ± 0.116
1.366TyrIle: 1.366 ± 0.266
1.056TyrLys: 1.056 ± 0.27
3.043TyrLeu: 3.043 ± 0.404
0.932TyrMet: 0.932 ± 0.263
0.869TyrAsn: 0.869 ± 0.224
1.49TyrPro: 1.49 ± 0.243
1.242TyrGln: 1.242 ± 0.295
2.298TyrArg: 2.298 ± 0.374
1.739TyrSer: 1.739 ± 0.301
2.112TyrThr: 2.112 ± 0.362
2.112TyrVal: 2.112 ± 0.432
0.311TyrTrp: 0.311 ± 0.154
0.745TyrTyr: 0.745 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (16103 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski