Amino acid dipepetide frequency for Cytophagales bacterium TFI 002

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.869AlaAla: 4.869 ± 0.094	0.66AlaCys: 0.66 ± 0.021	3.491AlaAsp: 3.491 ± 0.059	3.997AlaGlu: 3.997 ± 0.067	3.517AlaPhe: 3.517 ± 0.068	4.889AlaGly: 4.889 ± 0.075	0.994AlaHis: 0.994 ± 0.029	5.275AlaIle: 5.275 ± 0.071	4.681AlaLys: 4.681 ± 0.078	5.901AlaLeu: 5.901 ± 0.088	1.759AlaMet: 1.759 ± 0.04	3.863AlaAsn: 3.863 ± 0.066	2.146AlaPro: 2.146 ± 0.062	2.45AlaGln: 2.45 ± 0.042	2.065AlaArg: 2.065 ± 0.038	4.819AlaSer: 4.819 ± 0.101	4.151AlaThr: 4.151 ± 0.219	4.101AlaVal: 4.101 ± 0.066	0.721AlaTrp: 0.721 ± 0.021	2.587AlaTyr: 2.587 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.42CysAla: 0.42 ± 0.02	0.11CysCys: 0.11 ± 0.009	0.469CysAsp: 0.469 ± 0.027	0.546CysGlu: 0.546 ± 0.029	0.44CysPhe: 0.44 ± 0.017	0.667CysGly: 0.667 ± 0.046	0.201CysHis: 0.201 ± 0.012	0.588CysIle: 0.588 ± 0.02	0.465CysLys: 0.465 ± 0.017	0.698CysLeu: 0.698 ± 0.023	0.143CysMet: 0.143 ± 0.009	0.478CysAsn: 0.478 ± 0.032	0.382CysPro: 0.382 ± 0.023	0.284CysGln: 0.284 ± 0.016	0.254CysArg: 0.254 ± 0.012	0.704CysSer: 0.704 ± 0.039	0.61CysThr: 0.61 ± 0.052	0.482CysVal: 0.482 ± 0.021	0.083CysTrp: 0.083 ± 0.007	0.294CysTyr: 0.294 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.485AspAla: 3.485 ± 0.055	0.406AspCys: 0.406 ± 0.023	2.599AspAsp: 2.599 ± 0.07	3.525AspGlu: 3.525 ± 0.068	3.552AspPhe: 3.552 ± 0.059	4.143AspGly: 4.143 ± 0.081	0.921AspHis: 0.921 ± 0.024	4.085AspIle: 4.085 ± 0.066	3.855AspLys: 3.855 ± 0.059	5.371AspLeu: 5.371 ± 0.079	1.178AspMet: 1.178 ± 0.031	2.906AspAsn: 2.906 ± 0.055	2.094AspPro: 2.094 ± 0.049	1.832AspGln: 1.832 ± 0.036	1.964AspArg: 1.964 ± 0.042	3.261AspSer: 3.261 ± 0.083	2.464AspThr: 2.464 ± 0.139	3.199AspVal: 3.199 ± 0.057	0.821AspTrp: 0.821 ± 0.022	2.358AspTyr: 2.358 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	4.078GluAla: 4.078 ± 0.075	0.338GluCys: 0.338 ± 0.014	3.052GluAsp: 3.052 ± 0.045	4.278GluGlu: 4.278 ± 0.07	2.863GluPhe: 2.863 ± 0.053	3.923GluGly: 3.923 ± 0.067	1.005GluHis: 1.005 ± 0.03	5.177GluIle: 5.177 ± 0.067	5.481GluLys: 5.481 ± 0.077	6.261GluLeu: 6.261 ± 0.077	1.75GluMet: 1.75 ± 0.034	4.35GluAsn: 4.35 ± 0.067	1.729GluPro: 1.729 ± 0.037	2.095GluGln: 2.095 ± 0.046	2.391GluArg: 2.391 ± 0.047	3.764GluSer: 3.764 ± 0.06	3.157GluThr: 3.157 ± 0.054	4.485GluVal: 4.485 ± 0.084	0.726GluTrp: 0.726 ± 0.025	2.119GluTyr: 2.119 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	3.223PheAla: 3.223 ± 0.049	0.472PheCys: 0.472 ± 0.017	3.26PheAsp: 3.26 ± 0.054	3.559PheGlu: 3.559 ± 0.054	2.692PhePhe: 2.692 ± 0.063	3.837PheGly: 3.837 ± 0.059	0.813PheHis: 0.813 ± 0.026	3.541PheIle: 3.541 ± 0.063	3.38PheLys: 3.38 ± 0.06	4.806PheLeu: 4.806 ± 0.068	1.151PheMet: 1.151 ± 0.031	3.025PheAsn: 3.025 ± 0.055	1.806PhePro: 1.806 ± 0.041	1.718PheGln: 1.718 ± 0.04	1.701PheArg: 1.701 ± 0.038	3.996PheSer: 3.996 ± 0.068	3.042PheThr: 3.042 ± 0.058	3.245PheVal: 3.245 ± 0.058	0.667PheTrp: 0.667 ± 0.024	2.104PheTyr: 2.104 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.372GlyAla: 4.372 ± 0.074	0.704GlyCys: 0.704 ± 0.04	3.672GlyAsp: 3.672 ± 0.056	4.085GlyGlu: 4.085 ± 0.061	3.836GlyPhe: 3.836 ± 0.073	5.378GlyGly: 5.378 ± 0.099	1.177GlyHis: 1.177 ± 0.032	5.638GlyIle: 5.638 ± 0.07	5.204GlyLys: 5.204 ± 0.073	6.466GlyLeu: 6.466 ± 0.093	1.724GlyMet: 1.724 ± 0.037	4.125GlyAsn: 4.125 ± 0.074	1.733GlyPro: 1.733 ± 0.085	2.286GlyGln: 2.286 ± 0.054	2.358GlyArg: 2.358 ± 0.045	4.774GlySer: 4.774 ± 0.077	4.427GlyThr: 4.427 ± 0.147	4.653GlyVal: 4.653 ± 0.071	0.883GlyTrp: 0.883 ± 0.029	2.859GlyTyr: 2.859 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	0.935HisAla: 0.935 ± 0.026	0.173HisCys: 0.173 ± 0.013	0.745HisAsp: 0.745 ± 0.023	0.937HisGlu: 0.937 ± 0.029	1.076HisPhe: 1.076 ± 0.032	1.159HisGly: 1.159 ± 0.029	0.441HisHis: 0.441 ± 0.019	1.182HisIle: 1.182 ± 0.031	1.004HisLys: 1.004 ± 0.029	1.649HisLeu: 1.649 ± 0.032	0.318HisMet: 0.318 ± 0.016	0.878HisAsn: 0.878 ± 0.026	0.833HisPro: 0.833 ± 0.025	0.606HisGln: 0.606 ± 0.017	0.679HisArg: 0.679 ± 0.024	1.061HisSer: 1.061 ± 0.029	0.883HisThr: 0.883 ± 0.024	0.9HisVal: 0.9 ± 0.027	0.232HisTrp: 0.232 ± 0.013	0.692HisTyr: 0.692 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.584IleAla: 5.584 ± 0.066	0.72IleCys: 0.72 ± 0.026	4.576IleAsp: 4.576 ± 0.06	4.903IleGlu: 4.903 ± 0.064	3.5IlePhe: 3.5 ± 0.062	5.396IleGly: 5.396 ± 0.079	1.204IleHis: 1.204 ± 0.032	4.987IleIle: 4.987 ± 0.088	5.015IleLys: 5.015 ± 0.067	6.615IleLeu: 6.615 ± 0.09	1.323IleMet: 1.323 ± 0.031	4.266IleAsn: 4.266 ± 0.076	3.187IlePro: 3.187 ± 0.051	2.454IleGln: 2.454 ± 0.041	2.436IleArg: 2.436 ± 0.047	5.732IleSer: 5.732 ± 0.082	4.298IleThr: 4.298 ± 0.112	4.393IleVal: 4.393 ± 0.054	0.748IleTrp: 0.748 ± 0.022	2.586IleTyr: 2.586 ± 0.049	0.0IleXaa: 0.0 ± 0.0
Lys	5.009LysAla: 5.009 ± 0.076	0.369LysCys: 0.369 ± 0.018	4.077LysAsp: 4.077 ± 0.064	5.409LysGlu: 5.409 ± 0.081	2.628LysPhe: 2.628 ± 0.054	4.712LysGly: 4.712 ± 0.066	1.133LysHis: 1.133 ± 0.035	5.142LysIle: 5.142 ± 0.075	5.55LysLys: 5.55 ± 0.092	6.347LysLeu: 6.347 ± 0.083	1.905LysMet: 1.905 ± 0.04	4.477LysAsn: 4.477 ± 0.066	2.63LysPro: 2.63 ± 0.052	2.171LysGln: 2.171 ± 0.044	2.576LysArg: 2.576 ± 0.05	4.902LysSer: 4.902 ± 0.079	4.158LysThr: 4.158 ± 0.068	4.978LysVal: 4.978 ± 0.076	0.941LysTrp: 0.941 ± 0.03	2.899LysTyr: 2.899 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	6.463LeuAla: 6.463 ± 0.085	0.717LeuCys: 0.717 ± 0.024	5.054LeuAsp: 5.054 ± 0.074	5.699LeuGlu: 5.699 ± 0.079	4.768LeuPhe: 4.768 ± 0.074	6.107LeuGly: 6.107 ± 0.083	1.41LeuHis: 1.41 ± 0.032	6.631LeuIle: 6.631 ± 0.086	7.217LeuLys: 7.217 ± 0.087	8.657LeuLeu: 8.657 ± 0.119	2.019LeuMet: 2.019 ± 0.038	5.526LeuAsn: 5.526 ± 0.075	3.824LeuPro: 3.824 ± 0.086	2.908LeuGln: 2.908 ± 0.05	3.264LeuArg: 3.264 ± 0.054	7.162LeuSer: 7.162 ± 0.093	5.279LeuThr: 5.279 ± 0.106	5.611LeuVal: 5.611 ± 0.078	0.942LeuTrp: 0.942 ± 0.033	2.866LeuTyr: 2.866 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	1.72MetAla: 1.72 ± 0.039	0.136MetCys: 0.136 ± 0.011	1.234MetAsp: 1.234 ± 0.028	1.457MetGlu: 1.457 ± 0.036	0.812MetPhe: 0.812 ± 0.025	1.605MetGly: 1.605 ± 0.037	0.369MetHis: 0.369 ± 0.017	1.468MetIle: 1.468 ± 0.039	2.136MetLys: 2.136 ± 0.045	2.02MetLeu: 2.02 ± 0.04	0.57MetMet: 0.57 ± 0.022	1.382MetAsn: 1.382 ± 0.034	0.989MetPro: 0.989 ± 0.026	0.759MetGln: 0.759 ± 0.022	0.872MetArg: 0.872 ± 0.022	1.448MetSer: 1.448 ± 0.035	1.207MetThr: 1.207 ± 0.032	1.382MetVal: 1.382 ± 0.031	0.213MetTrp: 0.213 ± 0.012	0.649MetTyr: 0.649 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.807AsnAla: 3.807 ± 0.055	0.58AsnCys: 0.58 ± 0.034	3.052AsnAsp: 3.052 ± 0.063	3.743AsnGlu: 3.743 ± 0.053	3.114AsnPhe: 3.114 ± 0.065	4.833AsnGly: 4.833 ± 0.103	0.976AsnHis: 0.976 ± 0.026	4.317AsnIle: 4.317 ± 0.057	3.77AsnLys: 3.77 ± 0.067	5.355AsnLeu: 5.355 ± 0.074	1.214AsnMet: 1.214 ± 0.028	3.574AsnAsn: 3.574 ± 0.073	2.999AsnPro: 2.999 ± 0.057	2.11AsnGln: 2.11 ± 0.038	2.087AsnArg: 2.087 ± 0.038	4.42AsnSer: 4.42 ± 0.092	3.606AsnThr: 3.606 ± 0.132	3.431AsnVal: 3.431 ± 0.055	0.873AsnTrp: 0.873 ± 0.024	2.613AsnTyr: 2.613 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.529ProAla: 2.529 ± 0.082	0.235ProCys: 0.235 ± 0.013	2.143ProAsp: 2.143 ± 0.04	2.679ProGlu: 2.679 ± 0.051	2.036ProPhe: 2.036 ± 0.037	2.166ProGly: 2.166 ± 0.055	0.676ProHis: 0.676 ± 0.021	2.756ProIle: 2.756 ± 0.047	2.559ProLys: 2.559 ± 0.061	3.116ProLeu: 3.116 ± 0.055	0.765ProMet: 0.765 ± 0.024	2.569ProAsn: 2.569 ± 0.088	1.039ProPro: 1.039 ± 0.029	1.212ProGln: 1.212 ± 0.031	1.043ProArg: 1.043 ± 0.03	2.958ProSer: 2.958 ± 0.066	2.385ProThr: 2.385 ± 0.073	2.504ProVal: 2.504 ± 0.125	0.419ProTrp: 0.419 ± 0.017	1.463ProTyr: 1.463 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.164GlnAla: 2.164 ± 0.046	0.181GlnCys: 0.181 ± 0.011	1.57GlnAsp: 1.57 ± 0.032	2.17GlnGlu: 2.17 ± 0.041	1.666GlnPhe: 1.666 ± 0.036	2.016GlnGly: 2.016 ± 0.037	0.52GlnHis: 0.52 ± 0.02	2.523GlnIle: 2.523 ± 0.04	2.918GlnLys: 2.918 ± 0.047	3.136GlnLeu: 3.136 ± 0.051	0.819GlnMet: 0.819 ± 0.024	2.333GlnAsn: 2.333 ± 0.045	1.125GlnPro: 1.125 ± 0.04	1.223GlnGln: 1.223 ± 0.032	1.177GlnArg: 1.177 ± 0.028	2.282GlnSer: 2.282 ± 0.04	1.853GlnThr: 1.853 ± 0.05	2.146GlnVal: 2.146 ± 0.045	0.444GlnTrp: 0.444 ± 0.019	1.264GlnTyr: 1.264 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.146ArgAla: 2.146 ± 0.042	0.235ArgCys: 0.235 ± 0.013	1.733ArgAsp: 1.733 ± 0.037	2.087ArgGlu: 2.087 ± 0.042	1.902ArgPhe: 1.902 ± 0.045	2.083ArgGly: 2.083 ± 0.04	0.557ArgHis: 0.557 ± 0.023	2.826ArgIle: 2.826 ± 0.053	2.623ArgLys: 2.623 ± 0.046	3.16ArgLeu: 3.16 ± 0.054	0.934ArgMet: 0.934 ± 0.027	2.168ArgAsn: 2.168 ± 0.04	1.188ArgPro: 1.188 ± 0.03	1.049ArgGln: 1.049 ± 0.027	1.378ArgArg: 1.378 ± 0.038	2.16ArgSer: 2.16 ± 0.041	1.908ArgThr: 1.908 ± 0.039	2.241ArgVal: 2.241 ± 0.041	0.43ArgTrp: 0.43 ± 0.016	1.412ArgTyr: 1.412 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	4.161SerAla: 4.161 ± 0.11	0.748SerCys: 0.748 ± 0.032	3.724SerAsp: 3.724 ± 0.078	3.973SerGlu: 3.973 ± 0.061	4.256SerPhe: 4.256 ± 0.069	5.189SerGly: 5.189 ± 0.086	1.169SerHis: 1.169 ± 0.029	5.532SerIle: 5.532 ± 0.067	5.037SerLys: 5.037 ± 0.061	6.616SerLeu: 6.616 ± 0.065	1.435SerMet: 1.435 ± 0.03	4.305SerAsn: 4.305 ± 0.1	2.832SerPro: 2.832 ± 0.069	2.606SerGln: 2.606 ± 0.049	2.24SerArg: 2.24 ± 0.047	5.346SerSer: 5.346 ± 0.109	4.212SerThr: 4.212 ± 0.115	4.208SerVal: 4.208 ± 0.066	0.881SerTrp: 0.881 ± 0.026	2.901SerTyr: 2.901 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.384ThrAla: 4.384 ± 0.25	0.506ThrCys: 0.506 ± 0.045	3.109ThrAsp: 3.109 ± 0.089	3.094ThrGlu: 3.094 ± 0.057	2.995ThrPhe: 2.995 ± 0.055	4.482ThrGly: 4.482 ± 0.157	0.879ThrHis: 0.879 ± 0.023	4.583ThrIle: 4.583 ± 0.114	3.403ThrLys: 3.403 ± 0.056	5.223ThrLeu: 5.223 ± 0.072	1.003ThrMet: 1.003 ± 0.026	3.442ThrAsn: 3.442 ± 0.135	2.552ThrPro: 2.552 ± 0.126	1.902ThrGln: 1.902 ± 0.097	1.654ThrArg: 1.654 ± 0.035	4.095ThrSer: 4.095 ± 0.096	3.463ThrThr: 3.463 ± 0.142	3.836ThrVal: 3.836 ± 0.162	0.722ThrTrp: 0.722 ± 0.031	2.542ThrTyr: 2.542 ± 0.101	0.0ThrXaa: 0.0 ± 0.0
Val	4.356ValAla: 4.356 ± 0.079	0.586ValCys: 0.586 ± 0.029	3.474ValAsp: 3.474 ± 0.067	3.8ValGlu: 3.8 ± 0.061	3.404ValPhe: 3.404 ± 0.057	4.24ValGly: 4.24 ± 0.061	0.907ValHis: 0.907 ± 0.025	4.59ValIle: 4.59 ± 0.058	4.385ValLys: 4.385 ± 0.059	5.853ValLeu: 5.853 ± 0.081	1.359ValMet: 1.359 ± 0.029	3.747ValAsn: 3.747 ± 0.061	2.278ValPro: 2.278 ± 0.039	1.79ValGln: 1.79 ± 0.033	2.069ValArg: 2.069 ± 0.042	4.924ValSer: 4.924 ± 0.121	3.813ValThr: 3.813 ± 0.231	4.354ValVal: 4.354 ± 0.123	0.685ValTrp: 0.685 ± 0.041	2.28ValTyr: 2.28 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.742TrpAla: 0.742 ± 0.024	0.098TrpCys: 0.098 ± 0.009	0.714TrpAsp: 0.714 ± 0.021	0.745TrpGlu: 0.745 ± 0.024	0.589TrpPhe: 0.589 ± 0.018	0.856TrpGly: 0.856 ± 0.021	0.267TrpHis: 0.267 ± 0.014	0.768TrpIle: 0.768 ± 0.022	0.858TrpLys: 0.858 ± 0.026	1.128TrpLeu: 1.128 ± 0.032	0.334TrpMet: 0.334 ± 0.016	0.777TrpAsn: 0.777 ± 0.029	0.325TrpPro: 0.325 ± 0.016	0.525TrpGln: 0.525 ± 0.02	0.465TrpArg: 0.465 ± 0.019	0.886TrpSer: 0.886 ± 0.047	0.647TrpThr: 0.647 ± 0.042	0.744TrpVal: 0.744 ± 0.023	0.183TrpTrp: 0.183 ± 0.011	0.492TrpTyr: 0.492 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.487TyrAla: 2.487 ± 0.044	0.345TyrCys: 0.345 ± 0.016	2.272TyrAsp: 2.272 ± 0.047	2.335TyrGlu: 2.335 ± 0.052	2.39TyrPhe: 2.39 ± 0.054	2.672TyrGly: 2.672 ± 0.051	0.763TyrHis: 0.763 ± 0.023	2.316TyrIle: 2.316 ± 0.044	2.473TyrLys: 2.473 ± 0.047	3.749TyrLeu: 3.749 ± 0.065	0.722TyrMet: 0.722 ± 0.021	2.281TyrAsn: 2.281 ± 0.049	1.563TyrPro: 1.563 ± 0.043	1.552TyrGln: 1.552 ± 0.038	1.547TyrArg: 1.547 ± 0.032	2.676TyrSer: 2.676 ± 0.045	2.308TyrThr: 2.308 ± 0.077	2.0TyrVal: 2.0 ± 0.036	0.529TyrTrp: 0.529 ± 0.018	1.699TyrTyr: 1.699 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4431 proteins (1635812 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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