Streptococcus phage Javan330
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6AZJ7|A0A4D6AZJ7_9CAUD Cro/CI family transcriptional regulator OS=Streptococcus phage Javan330 OX=2548110 GN=Javan330_0033 PE=4 SV=1
MM1 pKa = 7.74 AEE3 pKa = 4.12 YY4 pKa = 11.04 VNQKK8 pKa = 8.82 TGATINTNTEE18 pKa = 3.23 ISGGDD23 pKa = 3.43 WVPIAAYY30 pKa = 9.47 KK31 pKa = 10.45 PLDD34 pKa = 3.77 SLTNAALKK42 pKa = 10.45 EE43 pKa = 3.93 ILDD46 pKa = 4.24 EE47 pKa = 5.73 KK48 pKa = 11.07 GITYY52 pKa = 10.14 DD53 pKa = 3.51 NRR55 pKa = 11.84 ATKK58 pKa = 10.34 PEE60 pKa = 4.98 LISLIEE66 pKa = 3.99 QADD69 pKa = 3.97 TEE71 pKa = 4.38 AQQ73 pKa = 3.26
Molecular weight: 7.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.449
IPC2_protein 4.355
IPC_protein 4.202
Toseland 4.024
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.037
Rodwell 4.037
Grimsley 3.948
Solomon 4.139
Lehninger 4.088
Nozaki 4.279
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.304
Patrickios 3.846
IPC_peptide 4.139
IPC2_peptide 4.291
IPC2.peptide.svr19 4.239
Protein with the highest isoelectric point:
>tr|A0A4D6B152|A0A4D6B152_9CAUD Hyaluronidase OS=Streptococcus phage Javan330 OX=2548110 GN=Javan330_0009 PE=4 SV=1
MM1 pKa = 7.97 EE2 pKa = 4.47 IRR4 pKa = 11.84 SYY6 pKa = 10.92 KK7 pKa = 9.77 KK8 pKa = 10.52 KK9 pKa = 10.54 NGEE12 pKa = 3.69 TAYY15 pKa = 10.08 MFRR18 pKa = 11.84 AYY20 pKa = 9.41 IGKK23 pKa = 9.64 INGYY27 pKa = 7.31 SQYY30 pKa = 9.8 ATRR33 pKa = 11.84 RR34 pKa = 11.84 GFATKK39 pKa = 10.44 AKK41 pKa = 10.21 ARR43 pKa = 11.84 AALLQLQDD51 pKa = 4.95 DD52 pKa = 4.46 IEE54 pKa = 5.14 SGEE57 pKa = 3.99 QSRR60 pKa = 11.84 KK61 pKa = 9.67 EE62 pKa = 3.66 ITVEE66 pKa = 4.15 EE67 pKa = 4.35 IAKK70 pKa = 9.72 KK71 pKa = 8.6 WLKK74 pKa = 10.69 DD75 pKa = 3.54 YY76 pKa = 11.6 SEE78 pKa = 4.59 TVQDD82 pKa = 3.18 STYY85 pKa = 10.82 IKK87 pKa = 9.75 TSRR90 pKa = 11.84 NFKK93 pKa = 8.78 NHH95 pKa = 6.67 IYY97 pKa = 10.21 PVFGNRR103 pKa = 11.84 KK104 pKa = 7.76 IASITPLQMQEE115 pKa = 4.15 QANEE119 pKa = 3.72 WSRR122 pKa = 11.84 KK123 pKa = 8.08 LVYY126 pKa = 9.94 GRR128 pKa = 11.84 KK129 pKa = 9.68 LKK131 pKa = 11.2 GLMNNVFKK139 pKa = 11.08 YY140 pKa = 10.47 AIRR143 pKa = 11.84 HH144 pKa = 5.65 GYY146 pKa = 9.54 IDD148 pKa = 4.17 TNPVDD153 pKa = 4.13 SVIASTRR160 pKa = 11.84 KK161 pKa = 10.06 KK162 pKa = 10.77 SDD164 pKa = 3.1 GKK166 pKa = 11.05 SDD168 pKa = 4.26 FYY170 pKa = 11.86 NKK172 pKa = 10.57 DD173 pKa = 3.25 EE174 pKa = 4.21 LQKK177 pKa = 10.67 FLKK180 pKa = 10.46 LVSKK184 pKa = 9.77 TKK186 pKa = 10.44 DD187 pKa = 3.21 LEE189 pKa = 4.81 KK190 pKa = 10.02 ITLFRR195 pKa = 11.84 LLAFTGARR203 pKa = 11.84 KK204 pKa = 10.34 GEE206 pKa = 3.97 ILALEE211 pKa = 4.27 WNDD214 pKa = 3.14 WTDD217 pKa = 2.97 NTLDD221 pKa = 3.25 INKK224 pKa = 10.21 AITRR228 pKa = 11.84 GFAGEE233 pKa = 4.32 EE234 pKa = 3.65 IGNTKK239 pKa = 8.91 TVSSNRR245 pKa = 11.84 LISLDD250 pKa = 3.71 KK251 pKa = 9.58 KK252 pKa = 9.13 TKK254 pKa = 10.64 NILKK258 pKa = 9.67 KK259 pKa = 9.44 WKK261 pKa = 9.47 KK262 pKa = 9.65 QNPNTKK268 pKa = 10.34 YY269 pKa = 10.04 IFEE272 pKa = 4.46 NEE274 pKa = 4.01 FKK276 pKa = 10.96 KK277 pKa = 10.35 PIPSTLPRR285 pKa = 11.84 KK286 pKa = 8.65 WLIKK290 pKa = 9.95 IVEE293 pKa = 4.42 GSDD296 pKa = 3.11 LRR298 pKa = 11.84 PIKK301 pKa = 10.35 IHH303 pKa = 5.85 GFRR306 pKa = 11.84 HH307 pKa = 4.67 THH309 pKa = 6.69 ASLCFDD315 pKa = 4.04 AGMTLKK321 pKa = 10.23 QVQHH325 pKa = 6.86 RR326 pKa = 11.84 LGHH329 pKa = 6.18 SDD331 pKa = 4.78 LKK333 pKa = 9.01 TTMNVYY339 pKa = 7.86 THH341 pKa = 6.12 ITKK344 pKa = 9.81 QAKK347 pKa = 9.97 DD348 pKa = 3.88 DD349 pKa = 3.51 IGEE352 pKa = 4.15 RR353 pKa = 11.84 FANYY357 pKa = 9.68 IDD359 pKa = 3.83 FF360 pKa = 4.89
Molecular weight: 41.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.014
IPC2_protein 9.238
IPC_protein 9.136
Toseland 10.072
ProMoST 9.648
Dawson 10.233
Bjellqvist 9.838
Wikipedia 10.365
Rodwell 10.847
Grimsley 10.277
Solomon 10.248
Lehninger 10.218
Nozaki 10.043
DTASelect 9.838
Thurlkill 10.087
EMBOSS 10.452
Sillero 10.131
Patrickios 10.482
IPC_peptide 10.248
IPC2_peptide 8.229
IPC2.peptide.svr19 8.247
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
10473
39
935
197.6
22.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.894 ± 0.519
0.582 ± 0.125
5.968 ± 0.208
7.639 ± 0.463
4.115 ± 0.263
6.913 ± 0.52
1.518 ± 0.182
6.894 ± 0.339
8.154 ± 0.452
8.107 ± 0.253
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.139
5.481 ± 0.237
3.046 ± 0.272
3.838 ± 0.154
4.469 ± 0.282
6.388 ± 0.27
5.891 ± 0.282
6.245 ± 0.262
1.365 ± 0.158
4.144 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here