Amino acid dipepetide frequency for Etheostoma spectabile (orangethroat darter)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.397AlaAla: 6.397 ± 0.046	1.339AlaCys: 1.339 ± 0.015	3.174AlaAsp: 3.174 ± 0.022	4.579AlaGlu: 4.579 ± 0.033	2.296AlaPhe: 2.296 ± 0.017	4.538AlaGly: 4.538 ± 0.033	1.568AlaHis: 1.568 ± 0.014	2.602AlaIle: 2.602 ± 0.018	3.322AlaLys: 3.322 ± 0.025	6.522AlaLeu: 6.522 ± 0.041	1.611AlaMet: 1.611 ± 0.014	2.175AlaAsn: 2.175 ± 0.015	3.694AlaPro: 3.694 ± 0.03	2.995AlaGln: 2.995 ± 0.024	3.191AlaArg: 3.191 ± 0.022	5.621AlaSer: 5.621 ± 0.033	3.622AlaThr: 3.622 ± 0.024	5.019AlaVal: 5.019 ± 0.033	0.694AlaTrp: 0.694 ± 0.011	1.43AlaTyr: 1.43 ± 0.016	0.003AlaXaa: 0.003 ± 0.001
Cys	1.203CysAla: 1.203 ± 0.012	0.739CysCys: 0.739 ± 0.011	1.094CysAsp: 1.094 ± 0.015	1.211CysGlu: 1.211 ± 0.017	0.888CysPhe: 0.888 ± 0.014	1.502CysGly: 1.502 ± 0.017	0.689CysHis: 0.689 ± 0.01	0.931CysIle: 0.931 ± 0.012	1.078CysLys: 1.078 ± 0.012	2.274CysLeu: 2.274 ± 0.024	0.499CysMet: 0.499 ± 0.008	0.831CysAsn: 0.831 ± 0.011	1.4CysPro: 1.4 ± 0.024	1.096CysGln: 1.096 ± 0.016	1.35CysArg: 1.35 ± 0.013	2.221CysSer: 2.221 ± 0.021	1.219CysThr: 1.219 ± 0.013	1.567CysVal: 1.567 ± 0.016	0.331CysTrp: 0.331 ± 0.007	0.612CysTyr: 0.612 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	2.891AspAla: 2.891 ± 0.021	1.13AspCys: 1.13 ± 0.014	3.003AspAsp: 3.003 ± 0.04	3.629AspGlu: 3.629 ± 0.027	1.977AspPhe: 1.977 ± 0.018	3.624AspGly: 3.624 ± 0.03	1.205AspHis: 1.205 ± 0.013	2.567AspIle: 2.567 ± 0.021	2.634AspLys: 2.634 ± 0.022	4.736AspLeu: 4.736 ± 0.03	1.271AspMet: 1.271 ± 0.013	1.974AspAsn: 1.974 ± 0.015	2.78AspPro: 2.78 ± 0.021	1.969AspGln: 1.969 ± 0.015	2.782AspArg: 2.782 ± 0.024	4.523AspSer: 4.523 ± 0.029	2.743AspThr: 2.743 ± 0.02	3.251AspVal: 3.251 ± 0.021	0.663AspTrp: 0.663 ± 0.01	1.431AspTyr: 1.431 ± 0.013	0.001AspXaa: 0.001 ± 0.0
Glu	4.616GluAla: 4.616 ± 0.034	1.181GluCys: 1.181 ± 0.018	4.374GluAsp: 4.374 ± 0.029	7.859GluGlu: 7.859 ± 0.078	1.822GluPhe: 1.822 ± 0.016	4.258GluGly: 4.258 ± 0.024	1.48GluHis: 1.48 ± 0.015	2.686GluIle: 2.686 ± 0.021	4.53GluLys: 4.53 ± 0.048	5.967GluLeu: 5.967 ± 0.043	1.737GluMet: 1.737 ± 0.018	2.63GluAsn: 2.63 ± 0.021	2.857GluPro: 2.857 ± 0.028	3.062GluGln: 3.062 ± 0.028	4.356GluArg: 4.356 ± 0.039	4.411GluSer: 4.411 ± 0.032	3.478GluThr: 3.478 ± 0.025	4.224GluVal: 4.224 ± 0.029	0.674GluTrp: 0.674 ± 0.009	1.479GluTyr: 1.479 ± 0.018	0.002GluXaa: 0.002 ± 0.0
Phe	1.83PheAla: 1.83 ± 0.017	0.905PheCys: 0.905 ± 0.011	1.655PheAsp: 1.655 ± 0.019	1.727PheGlu: 1.727 ± 0.016	1.521PhePhe: 1.521 ± 0.015	2.109PheGly: 2.109 ± 0.018	1.015PheHis: 1.015 ± 0.011	1.763PheIle: 1.763 ± 0.017	1.703PheLys: 1.703 ± 0.016	3.717PheLeu: 3.717 ± 0.029	0.775PheMet: 0.775 ± 0.009	1.394PheAsn: 1.394 ± 0.014	1.861PhePro: 1.861 ± 0.018	1.576PheGln: 1.576 ± 0.015	1.805PheArg: 1.805 ± 0.023	3.392PheSer: 3.392 ± 0.022	2.218PheThr: 2.218 ± 0.017	2.078PheVal: 2.078 ± 0.017	0.426PheTrp: 0.426 ± 0.007	1.117PheTyr: 1.117 ± 0.013	0.002PheXaa: 0.002 ± 0.0
Gly	4.187GlyAla: 4.187 ± 0.032	1.345GlyCys: 1.345 ± 0.014	3.213GlyAsp: 3.213 ± 0.023	4.169GlyGlu: 4.169 ± 0.031	2.325GlyPhe: 2.325 ± 0.021	5.73GlyGly: 5.73 ± 0.051	1.756GlyHis: 1.756 ± 0.018	2.503GlyIle: 2.503 ± 0.019	3.49GlyLys: 3.49 ± 0.024	5.67GlyLeu: 5.67 ± 0.034	1.524GlyMet: 1.524 ± 0.018	2.478GlyAsn: 2.478 ± 0.024	3.39GlyPro: 3.39 ± 0.04	2.902GlyGln: 2.902 ± 0.023	3.869GlyArg: 3.869 ± 0.03	5.878GlySer: 5.878 ± 0.035	3.462GlyThr: 3.462 ± 0.024	4.1GlyVal: 4.1 ± 0.028	0.815GlyTrp: 0.815 ± 0.012	1.794GlyTyr: 1.794 ± 0.02	0.004GlyXaa: 0.004 ± 0.001
His	1.402HisAla: 1.402 ± 0.014	0.778HisCys: 0.778 ± 0.012	0.993HisAsp: 0.993 ± 0.012	1.171HisGlu: 1.171 ± 0.013	1.052HisPhe: 1.052 ± 0.012	1.66HisGly: 1.66 ± 0.017	1.21HisHis: 1.21 ± 0.021	1.298HisIle: 1.298 ± 0.014	1.293HisLys: 1.293 ± 0.013	2.761HisLeu: 2.761 ± 0.02	0.67HisMet: 0.67 ± 0.01	1.075HisAsn: 1.075 ± 0.013	1.717HisPro: 1.717 ± 0.017	1.386HisGln: 1.386 ± 0.017	1.741HisArg: 1.741 ± 0.015	2.635HisSer: 2.635 ± 0.022	1.689HisThr: 1.689 ± 0.014	1.496HisVal: 1.496 ± 0.013	0.361HisTrp: 0.361 ± 0.006	0.844HisTyr: 0.844 ± 0.013	0.001HisXaa: 0.001 ± 0.0
Ile	2.372IleAla: 2.372 ± 0.02	1.044IleCys: 1.044 ± 0.012	1.943IleAsp: 1.943 ± 0.015	2.27IleGlu: 2.27 ± 0.021	1.628IlePhe: 1.628 ± 0.017	2.248IleGly: 2.248 ± 0.019	1.23IleHis: 1.23 ± 0.012	2.223IleIle: 2.223 ± 0.021	2.408IleLys: 2.408 ± 0.024	4.062IleLeu: 4.062 ± 0.029	1.022IleMet: 1.022 ± 0.011	1.856IleAsn: 1.856 ± 0.017	2.456IlePro: 2.456 ± 0.019	2.141IleGln: 2.141 ± 0.018	2.327IleArg: 2.327 ± 0.018	3.722IleSer: 3.722 ± 0.021	2.718IleThr: 2.718 ± 0.025	2.426IleVal: 2.426 ± 0.021	0.462IleTrp: 0.462 ± 0.009	1.244IleTyr: 1.244 ± 0.012	0.002IleXaa: 0.002 ± 0.0
Lys	3.742LysAla: 3.742 ± 0.027	0.963LysCys: 0.963 ± 0.012	3.171LysAsp: 3.171 ± 0.024	4.648LysGlu: 4.648 ± 0.042	1.407LysPhe: 1.407 ± 0.014	3.178LysGly: 3.178 ± 0.03	1.414LysHis: 1.414 ± 0.014	2.266LysIle: 2.266 ± 0.019	4.339LysLys: 4.339 ± 0.043	4.679LysLeu: 4.679 ± 0.028	1.465LysMet: 1.465 ± 0.015	2.144LysAsn: 2.144 ± 0.019	2.889LysPro: 2.889 ± 0.029	2.523LysGln: 2.523 ± 0.022	3.5LysArg: 3.5 ± 0.028	3.757LysSer: 3.757 ± 0.026	3.232LysThr: 3.232 ± 0.021	3.36LysVal: 3.36 ± 0.024	0.573LysTrp: 0.573 ± 0.008	1.372LysTyr: 1.372 ± 0.014	0.002LysXaa: 0.002 ± 0.0
Leu	6.139LeuAla: 6.139 ± 0.033	2.314LeuCys: 2.314 ± 0.018	4.706LeuAsp: 4.706 ± 0.034	6.122LeuGlu: 6.122 ± 0.048	3.341LeuPhe: 3.341 ± 0.022	5.263LeuGly: 5.263 ± 0.033	2.833LeuHis: 2.833 ± 0.023	3.66LeuIle: 3.66 ± 0.024	5.362LeuLys: 5.362 ± 0.034	10.299LeuLeu: 10.299 ± 0.065	2.143LeuMet: 2.143 ± 0.022	3.508LeuAsn: 3.508 ± 0.025	5.595LeuPro: 5.595 ± 0.033	5.473LeuGln: 5.473 ± 0.041	5.618LeuArg: 5.618 ± 0.033	8.644LeuSer: 8.644 ± 0.042	5.302LeuThr: 5.302 ± 0.025	5.479LeuVal: 5.479 ± 0.031	1.131LeuTrp: 1.131 ± 0.015	2.496LeuTyr: 2.496 ± 0.017	0.005LeuXaa: 0.005 ± 0.001
Met	1.947MetAla: 1.947 ± 0.014	0.507MetCys: 0.507 ± 0.012	1.453MetAsp: 1.453 ± 0.014	2.009MetGlu: 2.009 ± 0.018	0.851MetPhe: 0.851 ± 0.011	1.478MetGly: 1.478 ± 0.017	0.506MetHis: 0.506 ± 0.008	0.853MetIle: 0.853 ± 0.009	1.424MetLys: 1.424 ± 0.013	2.134MetLeu: 2.134 ± 0.019	0.73MetMet: 0.73 ± 0.011	0.875MetAsn: 0.875 ± 0.011	1.125MetPro: 1.125 ± 0.015	1.018MetGln: 1.018 ± 0.012	1.196MetArg: 1.196 ± 0.014	1.963MetSer: 1.963 ± 0.015	1.293MetThr: 1.293 ± 0.011	1.547MetVal: 1.547 ± 0.014	0.281MetTrp: 0.281 ± 0.007	0.593MetTyr: 0.593 ± 0.009	0.001MetXaa: 0.001 ± 0.0
Asn	2.124AsnAla: 2.124 ± 0.015	0.864AsnCys: 0.864 ± 0.014	1.599AsnAsp: 1.599 ± 0.014	2.016AsnGlu: 2.016 ± 0.019	1.317AsnPhe: 1.317 ± 0.015	2.726AsnGly: 2.726 ± 0.024	1.044AsnHis: 1.044 ± 0.013	2.036AsnIle: 2.036 ± 0.018	2.174AsnLys: 2.174 ± 0.018	3.407AsnLeu: 3.407 ± 0.024	1.052AsnMet: 1.052 ± 0.011	1.859AsnAsn: 1.859 ± 0.02	2.257AsnPro: 2.257 ± 0.019	1.732AsnGln: 1.732 ± 0.016	1.993AsnArg: 1.993 ± 0.016	3.292AsnSer: 3.292 ± 0.023	2.347AsnThr: 2.347 ± 0.017	2.283AsnVal: 2.283 ± 0.018	0.454AsnTrp: 0.454 ± 0.008	1.063AsnTyr: 1.063 ± 0.011	0.001AsnXaa: 0.001 ± 0.0
Pro	4.4ProAla: 4.4 ± 0.037	1.22ProCys: 1.22 ± 0.021	2.844ProAsp: 2.844 ± 0.022	3.757ProGlu: 3.757 ± 0.03	1.805ProPhe: 1.805 ± 0.016	4.165ProGly: 4.165 ± 0.047	1.614ProHis: 1.614 ± 0.018	1.935ProIle: 1.935 ± 0.019	2.56ProLys: 2.56 ± 0.027	5.227ProLeu: 5.227 ± 0.033	1.088ProMet: 1.088 ± 0.013	1.977ProAsn: 1.977 ± 0.019	5.703ProPro: 5.703 ± 0.065	2.808ProGln: 2.808 ± 0.028	2.912ProArg: 2.912 ± 0.022	5.954ProSer: 5.954 ± 0.043	3.273ProThr: 3.273 ± 0.027	3.921ProVal: 3.921 ± 0.028	0.586ProTrp: 0.586 ± 0.009	1.359ProTyr: 1.359 ± 0.015	0.003ProXaa: 0.003 ± 0.001
Gln	3.263GlnAla: 3.263 ± 0.025	1.005GlnCys: 1.005 ± 0.014	2.368GlnAsp: 2.368 ± 0.02	3.433GlnGlu: 3.433 ± 0.028	1.29GlnPhe: 1.29 ± 0.012	2.878GlnGly: 2.878 ± 0.025	1.494GlnHis: 1.494 ± 0.016	1.832GlnIle: 1.832 ± 0.016	2.513GlnLys: 2.513 ± 0.02	4.581GlnLeu: 4.581 ± 0.031	1.164GlnMet: 1.164 ± 0.014	1.722GlnAsn: 1.722 ± 0.015	2.8GlnPro: 2.8 ± 0.029	3.502GlnGln: 3.502 ± 0.062	3.261GlnArg: 3.261 ± 0.027	3.735GlnSer: 3.735 ± 0.028	2.734GlnThr: 2.734 ± 0.02	2.75GlnVal: 2.75 ± 0.02	0.555GlnTrp: 0.555 ± 0.008	1.159GlnTyr: 1.159 ± 0.012	0.002GlnXaa: 0.002 ± 0.0
Arg	3.615ArgAla: 3.615 ± 0.027	1.324ArgCys: 1.324 ± 0.018	2.966ArgAsp: 2.966 ± 0.021	4.092ArgGlu: 4.092 ± 0.039	1.839ArgPhe: 1.839 ± 0.017	3.781ArgGly: 3.781 ± 0.027	1.65ArgHis: 1.65 ± 0.015	2.242ArgIle: 2.242 ± 0.019	3.511ArgLys: 3.511 ± 0.026	5.386ArgLeu: 5.386 ± 0.031	1.334ArgMet: 1.334 ± 0.014	2.057ArgAsn: 2.057 ± 0.015	3.132ArgPro: 3.132 ± 0.028	2.776ArgGln: 2.776 ± 0.022	4.601ArgArg: 4.601 ± 0.032	4.691ArgSer: 4.691 ± 0.031	2.967ArgThr: 2.967 ± 0.018	3.363ArgVal: 3.363 ± 0.029	0.73ArgTrp: 0.73 ± 0.01	1.453ArgTyr: 1.453 ± 0.014	0.003ArgXaa: 0.003 ± 0.001
Ser	5.754SerAla: 5.754 ± 0.031	2.088SerCys: 2.088 ± 0.017	4.262SerAsp: 4.262 ± 0.027	4.875SerGlu: 4.875 ± 0.029	3.107SerPhe: 3.107 ± 0.019	5.716SerGly: 5.716 ± 0.033	2.404SerHis: 2.404 ± 0.02	3.356SerIle: 3.356 ± 0.022	4.043SerLys: 4.043 ± 0.024	8.631SerLeu: 8.631 ± 0.045	1.893SerMet: 1.893 ± 0.017	3.068SerAsn: 3.068 ± 0.022	6.406SerPro: 6.406 ± 0.053	4.126SerGln: 4.126 ± 0.03	4.717SerArg: 4.717 ± 0.03	11.021SerSer: 11.021 ± 0.086	5.14SerThr: 5.14 ± 0.031	5.563SerVal: 5.563 ± 0.027	1.053SerTrp: 1.053 ± 0.014	2.112SerTyr: 2.112 ± 0.018	0.004SerXaa: 0.004 ± 0.001
Thr	4.24ThrAla: 4.24 ± 0.024	1.339ThrCys: 1.339 ± 0.018	2.885ThrAsp: 2.885 ± 0.022	3.824ThrGlu: 3.824 ± 0.028	2.032ThrPhe: 2.032 ± 0.016	3.814ThrGly: 3.814 ± 0.028	1.478ThrHis: 1.478 ± 0.016	2.358ThrIle: 2.358 ± 0.018	2.697ThrLys: 2.697 ± 0.024	5.464ThrLeu: 5.464 ± 0.029	1.296ThrMet: 1.296 ± 0.015	1.994ThrAsn: 1.994 ± 0.017	3.829ThrPro: 3.829 ± 0.032	2.487ThrGln: 2.487 ± 0.018	2.613ThrArg: 2.613 ± 0.018	5.136ThrSer: 5.136 ± 0.033	3.64ThrThr: 3.64 ± 0.029	4.199ThrVal: 4.199 ± 0.031	0.706ThrTrp: 0.706 ± 0.013	1.378ThrTyr: 1.378 ± 0.012	0.004ThrXaa: 0.004 ± 0.001
Val	4.225ValAla: 4.225 ± 0.023	1.69ValCys: 1.69 ± 0.018	3.161ValAsp: 3.161 ± 0.018	4.104ValGlu: 4.104 ± 0.029	2.554ValPhe: 2.554 ± 0.025	3.653ValGly: 3.653 ± 0.028	1.583ValHis: 1.583 ± 0.016	2.829ValIle: 2.829 ± 0.022	3.452ValLys: 3.452 ± 0.024	6.224ValLeu: 6.224 ± 0.035	1.523ValMet: 1.523 ± 0.014	2.389ValAsn: 2.389 ± 0.019	3.435ValPro: 3.435 ± 0.023	2.777ValGln: 2.777 ± 0.019	3.274ValArg: 3.274 ± 0.018	5.467ValSer: 5.467 ± 0.033	3.985ValThr: 3.985 ± 0.029	4.504ValVal: 4.504 ± 0.034	0.782ValTrp: 0.782 ± 0.009	1.729ValTyr: 1.729 ± 0.015	0.004ValXaa: 0.004 ± 0.001
Trp	0.701TrpAla: 0.701 ± 0.009	0.256TrpCys: 0.256 ± 0.006	0.649TrpAsp: 0.649 ± 0.01	0.757TrpGlu: 0.757 ± 0.01	0.446TrpPhe: 0.446 ± 0.008	0.686TrpGly: 0.686 ± 0.013	0.276TrpHis: 0.276 ± 0.005	0.538TrpIle: 0.538 ± 0.01	0.722TrpLys: 0.722 ± 0.01	1.187TrpLeu: 1.187 ± 0.014	0.341TrpMet: 0.341 ± 0.007	0.486TrpAsn: 0.486 ± 0.009	0.474TrpPro: 0.474 ± 0.008	0.49TrpGln: 0.49 ± 0.008	0.837TrpArg: 0.837 ± 0.011	0.99TrpSer: 0.99 ± 0.012	0.751TrpThr: 0.751 ± 0.013	0.71TrpVal: 0.71 ± 0.012	0.194TrpTrp: 0.194 ± 0.005	0.338TrpTyr: 0.338 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.342TyrAla: 1.342 ± 0.014	0.704TyrCys: 0.704 ± 0.013	1.266TyrAsp: 1.266 ± 0.012	1.432TyrGlu: 1.432 ± 0.013	1.087TyrPhe: 1.087 ± 0.014	1.594TyrGly: 1.594 ± 0.015	0.782TyrHis: 0.782 ± 0.01	1.324TyrIle: 1.324 ± 0.015	1.37TyrLys: 1.37 ± 0.015	2.499TyrLeu: 2.499 ± 0.021	0.645TyrMet: 0.645 ± 0.01	1.146TyrAsn: 1.146 ± 0.011	1.305TyrPro: 1.305 ± 0.015	1.197TyrGln: 1.197 ± 0.012	1.57TyrArg: 1.57 ± 0.014	2.283TyrSer: 2.283 ± 0.019	1.597TyrThr: 1.597 ± 0.015	1.501TyrVal: 1.501 ± 0.017	0.359TyrTrp: 0.359 ± 0.007	0.921TyrTyr: 0.921 ± 0.011	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.003XaaGlu: 0.003 ± 0.001	0.001XaaPhe: 0.001 ± 0.0	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.0	0.002XaaLys: 0.002 ± 0.0	0.004XaaLeu: 0.004 ± 0.001	0.002XaaMet: 0.002 ± 0.0	0.002XaaAsn: 0.002 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.003XaaArg: 0.003 ± 0.001	0.005XaaSer: 0.005 ± 0.001	0.003XaaThr: 0.003 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.002XaaTyr: 0.002 ± 0.0	0.134XaaXaa: 0.134 ± 0.008

Statistics based on 18371 proteins (8925540 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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