Microbacterium sp. T32

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A163GY74|A0A163GY74_9MICO Phosphoglycerate kinase OS=Microbacterium sp. T32 OX=1776083 GN=pgk PE=3 SV=1
MM1 pKa = 7.3TPEE4 pKa = 3.59QARR7 pKa = 11.84AQTLEE12 pKa = 3.85ILQATIAVSGASDD25 pKa = 3.24WVLDD29 pKa = 4.13RR30 pKa = 11.84DD31 pKa = 5.03DD32 pKa = 4.79VPLPQQCEE40 pKa = 3.68IDD42 pKa = 3.81GKK44 pKa = 10.94EE45 pKa = 3.72GVAFNHH51 pKa = 6.36GGYY54 pKa = 10.62AVTEE58 pKa = 4.45GADD61 pKa = 3.54PAADD65 pKa = 3.52AQRR68 pKa = 11.84VADD71 pKa = 3.7YY72 pKa = 7.76WASVGIDD79 pKa = 3.23ARR81 pKa = 11.84VVSDD85 pKa = 3.44PVPRR89 pKa = 11.84VFGGGGPVDD98 pKa = 5.19AISFSTSPGYY108 pKa = 9.25TISVGGTCVPGNAFDD123 pKa = 5.06YY124 pKa = 11.34YY125 pKa = 11.3DD126 pKa = 4.61DD127 pKa = 4.43TPTPAPSSS135 pKa = 3.61

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165I852|A0A165I852_9MICO Translation initiation factor IF-1 OS=Microbacterium sp. T32 OX=1776083 GN=infA PE=3 SV=1
MM1 pKa = 6.84TRR3 pKa = 11.84SRR5 pKa = 11.84LRR7 pKa = 11.84RR8 pKa = 11.84ILVLLAPVAAVVVLLVVVHH27 pKa = 6.24GAGIPLTVPGVIVVVLLLVVGRR49 pKa = 11.84LVFAVTRR56 pKa = 11.84KK57 pKa = 9.4RR58 pKa = 11.84RR59 pKa = 11.84AGRR62 pKa = 11.84RR63 pKa = 3.41

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3267

0

3267

1023341

38

1987

313.2

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.884 ± 0.075

0.455 ± 0.009

6.462 ± 0.038

5.433 ± 0.048

3.13 ± 0.028

8.903 ± 0.037

1.923 ± 0.022

4.326 ± 0.028

1.829 ± 0.034

10.063 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.646 ± 0.016

1.859 ± 0.024

5.567 ± 0.035

2.601 ± 0.024

7.628 ± 0.053

5.461 ± 0.031

6.112 ± 0.039

9.246 ± 0.042

1.537 ± 0.021

1.937 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski