Prauserella marina

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Prauserella

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222VRQ5|A0A222VRQ5_9PSEU Uncharacterized protein OS=Prauserella marina OX=530584 GN=DES30_108180 PE=4 SV=1
MM1 pKa = 7.88AIRR4 pKa = 11.84RR5 pKa = 11.84GSAALLSLLVALVWLVPSAFADD27 pKa = 3.78DD28 pKa = 4.74AKK30 pKa = 11.13DD31 pKa = 3.2AGRR34 pKa = 11.84PSVPQTQQVQRR45 pKa = 11.84AQVQPAQAQVLTWTGNNSLNEE66 pKa = 4.04YY67 pKa = 10.73GEE69 pKa = 4.74APTTATAGAATLVFEE84 pKa = 4.65NSVATGNTSGMTHH97 pKa = 6.95TLTFDD102 pKa = 3.29TSTPGYY108 pKa = 9.66NHH110 pKa = 7.2DD111 pKa = 3.63VDD113 pKa = 3.99VDD115 pKa = 3.85IVASPFDD122 pKa = 4.2ANGGRR127 pKa = 11.84HH128 pKa = 4.82EE129 pKa = 4.32VQVNLSPGKK138 pKa = 10.47YY139 pKa = 9.0RR140 pKa = 11.84FFCAIPGHH148 pKa = 5.17QQMQGVLTVTEE159 pKa = 4.69GGEE162 pKa = 4.12QDD164 pKa = 3.29TTAPTVTAEE173 pKa = 4.01VTGDD177 pKa = 3.08QDD179 pKa = 5.09AEE181 pKa = 4.29GNYY184 pKa = 10.09LGSATVSVSAEE195 pKa = 3.88DD196 pKa = 3.96AEE198 pKa = 4.66SGVAGIEE205 pKa = 4.24YY206 pKa = 10.47AVGEE210 pKa = 4.67GDD212 pKa = 3.63FQPYY216 pKa = 9.8SEE218 pKa = 4.82PVTLAEE224 pKa = 4.69PGDD227 pKa = 3.71YY228 pKa = 9.53TVQYY232 pKa = 10.36RR233 pKa = 11.84ATDD236 pKa = 3.59NAGNTSDD243 pKa = 4.03PGSVSFTVAEE253 pKa = 4.78GEE255 pKa = 4.55PEE257 pKa = 4.83DD258 pKa = 3.82NTPPEE263 pKa = 4.25VTAEE267 pKa = 3.95LAGNQDD273 pKa = 2.76ADD275 pKa = 3.83GNYY278 pKa = 10.2LGAATVILNAQDD290 pKa = 3.56AGSGVAGIEE299 pKa = 3.85YY300 pKa = 10.41DD301 pKa = 3.54LDD303 pKa = 3.87GAGFADD309 pKa = 3.81YY310 pKa = 10.87SEE312 pKa = 4.3PVRR315 pKa = 11.84VTEE318 pKa = 4.55LGEE321 pKa = 3.88HH322 pKa = 4.92TVAYY326 pKa = 9.7RR327 pKa = 11.84ATDD330 pKa = 3.21NAGNVSEE337 pKa = 5.07AGSATFTVIEE347 pKa = 4.75GSQDD351 pKa = 3.36DD352 pKa = 4.25TTPPHH357 pKa = 6.93VMAEE361 pKa = 4.11VTGQQDD367 pKa = 2.91GDD369 pKa = 3.86GNYY372 pKa = 10.15VGSAAVNLTAHH383 pKa = 6.83DD384 pKa = 4.39AEE386 pKa = 4.86SGVDD390 pKa = 3.46TVEE393 pKa = 3.93YY394 pKa = 10.73SLDD397 pKa = 3.11GGMYY401 pKa = 9.3TPYY404 pKa = 9.84TEE406 pKa = 4.25PVVVSAIGSHH416 pKa = 3.98TVEE419 pKa = 4.43YY420 pKa = 10.39RR421 pKa = 11.84ATDD424 pKa = 3.57LAGNTSEE431 pKa = 4.47PGSVSFAVVEE441 pKa = 4.17GQPDD445 pKa = 3.48DD446 pKa = 3.61TAPTVTAEE454 pKa = 4.15VTGEE458 pKa = 3.81QDD460 pKa = 3.14GDD462 pKa = 3.75GAYY465 pKa = 10.53LGAATVVISAEE476 pKa = 4.05DD477 pKa = 3.66AEE479 pKa = 4.77SGVASVEE486 pKa = 4.19YY487 pKa = 10.7ALGEE491 pKa = 4.45SEE493 pKa = 4.12FTGYY497 pKa = 9.13TDD499 pKa = 3.25PVEE502 pKa = 4.06VTEE505 pKa = 4.61PGEE508 pKa = 3.88HH509 pKa = 4.44TVAYY513 pKa = 9.65RR514 pKa = 11.84ATDD517 pKa = 3.21NAGNVSEE524 pKa = 4.75AGSVTFTVVEE534 pKa = 4.91GSQEE538 pKa = 3.91DD539 pKa = 3.92TTAPEE544 pKa = 3.57TSIRR548 pKa = 11.84ITGEE552 pKa = 3.78QDD554 pKa = 3.15WEE556 pKa = 3.72WSYY559 pKa = 11.97IDD561 pKa = 4.0LAKK564 pKa = 9.61ITLLAEE570 pKa = 4.66DD571 pKa = 5.08DD572 pKa = 4.08EE573 pKa = 5.64SGVALIEE580 pKa = 4.01YY581 pKa = 10.25SFGEE585 pKa = 4.56GSFRR589 pKa = 11.84PYY591 pKa = 10.46RR592 pKa = 11.84IPLTVLDD599 pKa = 4.32PGEE602 pKa = 4.26YY603 pKa = 7.63TVHH606 pKa = 6.42YY607 pKa = 9.63RR608 pKa = 11.84ATDD611 pKa = 3.41NAGNTSEE618 pKa = 4.46VGTARR623 pKa = 11.84FRR625 pKa = 11.84IVADD629 pKa = 4.21GVHH632 pKa = 5.81CTVSDD637 pKa = 3.35ARR639 pKa = 11.84EE640 pKa = 4.04TVVVGDD646 pKa = 3.93TDD648 pKa = 3.58TGVANVDD655 pKa = 3.53TGNGCTINDD664 pKa = 5.3LIVEE668 pKa = 4.25EE669 pKa = 5.02AGYY672 pKa = 9.45PDD674 pKa = 3.08QAAFVSHH681 pKa = 5.83VRR683 pKa = 11.84QVARR687 pKa = 11.84DD688 pKa = 3.77LEE690 pKa = 4.38AQGVITAQEE699 pKa = 3.76RR700 pKa = 11.84KK701 pKa = 10.02AIVSAARR708 pKa = 11.84GVPVPSAAA716 pKa = 3.86

Molecular weight: 74.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6P460|A0A1G6P460_9PSEU UPF0336 protein DES30_102934 OS=Prauserella marina OX=530584 GN=DES30_102934 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.71QGKK33 pKa = 8.65

Molecular weight: 4.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6551

0

6551

2104163

28

27313

321.2

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.102 ± 0.038

0.741 ± 0.013

5.914 ± 0.027

6.122 ± 0.028

2.904 ± 0.013

9.401 ± 0.028

2.148 ± 0.014

3.67 ± 0.021

2.138 ± 0.02

10.442 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.774 ± 0.013

1.942 ± 0.014

5.676 ± 0.026

2.618 ± 0.018

7.798 ± 0.034

5.362 ± 0.019

6.017 ± 0.021

8.833 ± 0.027

1.447 ± 0.012

1.951 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski