Sphingomonas guangdongensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285QYB7|A0A285QYB7_9SPHN Outer membrane protein adhesin transport system OS=Sphingomonas guangdongensis OX=1141890 GN=SAMN06297144_1932 PE=3 SV=1
MM1 pKa = 7.63PNDD4 pKa = 3.34HH5 pKa = 7.13EE6 pKa = 4.61YY7 pKa = 11.46AFDD10 pKa = 3.66SYY12 pKa = 11.55GDD14 pKa = 4.1EE15 pKa = 4.11EE16 pKa = 5.58SEE18 pKa = 4.19PQDD21 pKa = 4.86ASDD24 pKa = 5.03FDD26 pKa = 6.11DD27 pKa = 4.67GDD29 pKa = 4.52AYY31 pKa = 11.1DD32 pKa = 5.49DD33 pKa = 4.23ADD35 pKa = 4.09ADD37 pKa = 4.0EE38 pKa = 4.88EE39 pKa = 4.46DD40 pKa = 4.27RR41 pKa = 11.84TDD43 pKa = 3.83LGPDD47 pKa = 3.34GVVRR51 pKa = 11.84CDD53 pKa = 3.53FRR55 pKa = 11.84DD56 pKa = 3.7ANGEE60 pKa = 3.87RR61 pKa = 11.84CPNYY65 pKa = 10.37LRR67 pKa = 11.84TDD69 pKa = 3.46EE70 pKa = 5.1PGAGLDD76 pKa = 3.72FNFCEE81 pKa = 4.18EE82 pKa = 4.34HH83 pKa = 6.44TDD85 pKa = 3.71HH86 pKa = 7.05EE87 pKa = 4.84VARR90 pKa = 4.58

Molecular weight: 10.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285QAS2|A0A285QAS2_9SPHN Uncharacterized protein OS=Sphingomonas guangdongensis OX=1141890 GN=SAMN06297144_0314 PE=4 SV=1
MM1 pKa = 7.41AARR4 pKa = 11.84RR5 pKa = 11.84ASPSAVKK12 pKa = 10.6LNRR15 pKa = 11.84TYY17 pKa = 11.39DD18 pKa = 3.67VAEE21 pKa = 3.9LAARR25 pKa = 11.84LGVHH29 pKa = 6.53KK30 pKa = 10.11NTVRR34 pKa = 11.84HH35 pKa = 4.67WQRR38 pKa = 11.84DD39 pKa = 3.39GLKK42 pKa = 9.69PLDD45 pKa = 3.46GRR47 pKa = 11.84RR48 pKa = 11.84PVLFHH53 pKa = 6.78GSTVRR58 pKa = 11.84AFLSSRR64 pKa = 11.84SASRR68 pKa = 11.84KK69 pKa = 9.26SPCPPGTLYY78 pKa = 10.8CFRR81 pKa = 11.84CRR83 pKa = 11.84APRR86 pKa = 11.84APALGMVDD94 pKa = 4.09YY95 pKa = 10.95LPISATGGNVRR106 pKa = 11.84AICATCEE113 pKa = 4.02TVMHH117 pKa = 6.54RR118 pKa = 11.84RR119 pKa = 11.84AAQATLASILPGCDD133 pKa = 3.07VQVVQAPPRR142 pKa = 11.84LKK144 pKa = 10.68GSPSPSLDD152 pKa = 3.25CDD154 pKa = 4.09LEE156 pKa = 4.36RR157 pKa = 11.84QATTT161 pKa = 3.34

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3454

0

3454

1075973

27

2486

311.5

33.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.533 ± 0.072

0.699 ± 0.011

5.934 ± 0.032

5.065 ± 0.038

3.334 ± 0.025

9.032 ± 0.046

1.846 ± 0.023

4.421 ± 0.03

2.343 ± 0.029

10.141 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.062 ± 0.021

2.323 ± 0.036

5.485 ± 0.036

2.949 ± 0.021

7.985 ± 0.048

4.957 ± 0.031

5.606 ± 0.039

7.731 ± 0.031

1.445 ± 0.02

2.108 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski