Amino acid dipepetide frequency for Pseudomonas phage UNO-SLW4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.309AlaAla: 11.309 ± 1.301
0.656AlaCys: 0.656 ± 0.272
6.146AlaAsp: 6.146 ± 0.854
6.146AlaGlu: 6.146 ± 0.633
3.442AlaPhe: 3.442 ± 0.631
7.785AlaGly: 7.785 ± 0.797
2.213AlaHis: 2.213 ± 0.479
3.606AlaIle: 3.606 ± 0.558
6.31AlaLys: 6.31 ± 0.461
8.768AlaLeu: 8.768 ± 1.039
3.524AlaMet: 3.524 ± 0.674
4.097AlaAsn: 4.097 ± 0.663
3.442AlaPro: 3.442 ± 0.589
5.736AlaGln: 5.736 ± 0.995
6.474AlaArg: 6.474 ± 0.7
7.129AlaSer: 7.129 ± 1.0
4.835AlaThr: 4.835 ± 1.238
6.556AlaVal: 6.556 ± 0.657
1.311AlaTrp: 1.311 ± 0.433
3.196AlaTyr: 3.196 ± 0.547
0.0AlaXaa: 0.0 ± 0.0
Cys
1.065CysAla: 1.065 ± 0.322
0.0CysCys: 0.0 ± 0.0
0.656CysAsp: 0.656 ± 0.318
0.492CysGlu: 0.492 ± 0.158
0.41CysPhe: 0.41 ± 0.213
0.819CysGly: 0.819 ± 0.29
0.164CysHis: 0.164 ± 0.101
0.246CysIle: 0.246 ± 0.132
0.492CysLys: 0.492 ± 0.211
1.065CysLeu: 1.065 ± 0.315
0.246CysMet: 0.246 ± 0.128
0.082CysAsn: 0.082 ± 0.071
0.164CysPro: 0.164 ± 0.11
0.41CysGln: 0.41 ± 0.181
0.574CysArg: 0.574 ± 0.2
0.328CysSer: 0.328 ± 0.13
0.328CysThr: 0.328 ± 0.162
0.328CysVal: 0.328 ± 0.162
0.164CysTrp: 0.164 ± 0.117
0.246CysTyr: 0.246 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
6.474AspAla: 6.474 ± 0.752
0.41AspCys: 0.41 ± 0.161
4.671AspAsp: 4.671 ± 0.642
3.524AspGlu: 3.524 ± 0.561
3.114AspPhe: 3.114 ± 0.424
6.802AspGly: 6.802 ± 0.698
1.475AspHis: 1.475 ± 0.364
3.442AspIle: 3.442 ± 0.406
3.196AspLys: 3.196 ± 0.48
4.179AspLeu: 4.179 ± 0.652
1.885AspMet: 1.885 ± 0.448
1.475AspAsn: 1.475 ± 0.287
3.278AspPro: 3.278 ± 0.689
2.376AspGln: 2.376 ± 0.334
3.933AspArg: 3.933 ± 0.735
3.032AspSer: 3.032 ± 0.563
3.032AspThr: 3.032 ± 0.435
3.852AspVal: 3.852 ± 0.641
1.229AspTrp: 1.229 ± 0.354
1.721AspTyr: 1.721 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
8.522GluAla: 8.522 ± 0.829
0.819GluCys: 0.819 ± 0.323
4.179GluAsp: 4.179 ± 0.425
5.163GluGlu: 5.163 ± 0.907
2.458GluPhe: 2.458 ± 0.454
5.163GluGly: 5.163 ± 0.64
1.557GluHis: 1.557 ± 0.345
2.868GluIle: 2.868 ± 0.385
3.36GluLys: 3.36 ± 0.451
5.736GluLeu: 5.736 ± 0.813
1.967GluMet: 1.967 ± 0.448
2.786GluAsn: 2.786 ± 0.358
2.131GluPro: 2.131 ± 0.384
2.295GluGln: 2.295 ± 0.369
3.852GluArg: 3.852 ± 0.475
3.524GluSer: 3.524 ± 0.488
4.425GluThr: 4.425 ± 0.456
4.179GluVal: 4.179 ± 0.721
1.393GluTrp: 1.393 ± 0.397
2.213GluTyr: 2.213 ± 0.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.049PheAla: 2.049 ± 0.504
0.492PheCys: 0.492 ± 0.239
3.032PheAsp: 3.032 ± 0.657
2.131PheGlu: 2.131 ± 0.407
1.393PhePhe: 1.393 ± 0.294
2.95PheGly: 2.95 ± 0.475
0.819PheHis: 0.819 ± 0.217
1.311PheIle: 1.311 ± 0.385
2.131PheLys: 2.131 ± 0.389
3.688PheLeu: 3.688 ± 0.526
1.311PheMet: 1.311 ± 0.224
1.885PheAsn: 1.885 ± 0.403
1.557PhePro: 1.557 ± 0.436
0.738PheGln: 0.738 ± 0.216
2.376PheArg: 2.376 ± 0.37
1.639PheSer: 1.639 ± 0.312
2.54PheThr: 2.54 ± 0.353
2.131PheVal: 2.131 ± 0.401
0.574PheTrp: 0.574 ± 0.199
0.819PheTyr: 0.819 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
7.375GlyAla: 7.375 ± 1.277
0.901GlyCys: 0.901 ± 0.297
4.917GlyAsp: 4.917 ± 0.493
5.572GlyGlu: 5.572 ± 0.695
3.77GlyPhe: 3.77 ± 0.637
7.129GlyGly: 7.129 ± 0.872
1.639GlyHis: 1.639 ± 0.244
4.507GlyIle: 4.507 ± 0.773
4.507GlyLys: 4.507 ± 0.771
6.966GlyLeu: 6.966 ± 0.847
2.049GlyMet: 2.049 ± 0.502
3.032GlyAsn: 3.032 ± 0.505
2.54GlyPro: 2.54 ± 0.578
2.54GlyGln: 2.54 ± 0.383
5.49GlyArg: 5.49 ± 0.712
5.409GlySer: 5.409 ± 0.617
4.097GlyThr: 4.097 ± 0.5
5.409GlyVal: 5.409 ± 0.593
1.475GlyTrp: 1.475 ± 0.319
3.114GlyTyr: 3.114 ± 0.558
0.0GlyXaa: 0.0 ± 0.0
His
1.557HisAla: 1.557 ± 0.35
0.41HisCys: 0.41 ± 0.162
1.229HisAsp: 1.229 ± 0.366
1.721HisGlu: 1.721 ± 0.385
0.901HisPhe: 0.901 ± 0.239
1.885HisGly: 1.885 ± 0.397
0.492HisHis: 0.492 ± 0.197
0.901HisIle: 0.901 ± 0.218
1.311HisLys: 1.311 ± 0.282
2.295HisLeu: 2.295 ± 0.376
0.492HisMet: 0.492 ± 0.179
0.983HisAsn: 0.983 ± 0.346
0.738HisPro: 0.738 ± 0.252
0.738HisGln: 0.738 ± 0.18
1.393HisArg: 1.393 ± 0.322
1.065HisSer: 1.065 ± 0.25
1.147HisThr: 1.147 ± 0.254
1.639HisVal: 1.639 ± 0.379
0.656HisTrp: 0.656 ± 0.193
0.901HisTyr: 0.901 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
3.442IleAla: 3.442 ± 0.479
0.574IleCys: 0.574 ± 0.199
2.95IleAsp: 2.95 ± 0.455
3.688IleGlu: 3.688 ± 0.586
0.901IlePhe: 0.901 ± 0.226
3.196IleGly: 3.196 ± 0.519
0.901IleHis: 0.901 ± 0.356
2.295IleIle: 2.295 ± 0.422
2.868IleLys: 2.868 ± 0.536
3.852IleLeu: 3.852 ± 0.514
1.393IleMet: 1.393 ± 0.364
1.311IleAsn: 1.311 ± 0.346
2.458IlePro: 2.458 ± 0.388
2.131IleGln: 2.131 ± 0.433
3.114IleArg: 3.114 ± 0.317
1.885IleSer: 1.885 ± 0.364
2.786IleThr: 2.786 ± 0.412
3.278IleVal: 3.278 ± 0.433
0.328IleTrp: 0.328 ± 0.146
1.803IleTyr: 1.803 ± 0.527
0.0IleXaa: 0.0 ± 0.0
Lys
8.195LysAla: 8.195 ± 0.843
0.492LysCys: 0.492 ± 0.203
4.261LysAsp: 4.261 ± 0.571
4.015LysGlu: 4.015 ± 0.539
2.54LysPhe: 2.54 ± 0.328
4.917LysGly: 4.917 ± 0.618
1.721LysHis: 1.721 ± 0.432
2.376LysIle: 2.376 ± 0.356
3.196LysLys: 3.196 ± 0.753
4.999LysLeu: 4.999 ± 0.676
1.721LysMet: 1.721 ± 0.305
1.967LysAsn: 1.967 ± 0.454
2.54LysPro: 2.54 ± 0.493
2.213LysGln: 2.213 ± 0.422
3.36LysArg: 3.36 ± 0.712
2.54LysSer: 2.54 ± 0.332
3.524LysThr: 3.524 ± 0.517
3.606LysVal: 3.606 ± 0.602
0.574LysTrp: 0.574 ± 0.188
1.147LysTyr: 1.147 ± 0.225
0.0LysXaa: 0.0 ± 0.0
Leu
8.441LeuAla: 8.441 ± 0.95
0.164LeuCys: 0.164 ± 0.107
4.999LeuAsp: 4.999 ± 0.658
6.31LeuGlu: 6.31 ± 0.811
2.376LeuPhe: 2.376 ± 0.537
5.327LeuGly: 5.327 ± 0.651
2.049LeuHis: 2.049 ± 0.379
3.688LeuIle: 3.688 ± 0.549
7.129LeuLys: 7.129 ± 0.664
5.9LeuLeu: 5.9 ± 0.901
2.786LeuMet: 2.786 ± 0.467
3.852LeuAsn: 3.852 ± 0.503
2.786LeuPro: 2.786 ± 0.445
4.097LeuGln: 4.097 ± 0.445
4.507LeuArg: 4.507 ± 0.474
4.999LeuSer: 4.999 ± 0.695
5.163LeuThr: 5.163 ± 0.846
5.9LeuVal: 5.9 ± 0.555
1.065LeuTrp: 1.065 ± 0.268
1.885LeuTyr: 1.885 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
3.933MetAla: 3.933 ± 0.469
0.164MetCys: 0.164 ± 0.11
1.639MetAsp: 1.639 ± 0.406
2.54MetGlu: 2.54 ± 0.562
0.328MetPhe: 0.328 ± 0.165
2.54MetGly: 2.54 ± 0.438
0.574MetHis: 0.574 ± 0.194
1.393MetIle: 1.393 ± 0.262
1.147MetLys: 1.147 ± 0.289
2.622MetLeu: 2.622 ± 0.404
0.656MetMet: 0.656 ± 0.224
1.229MetAsn: 1.229 ± 0.294
1.393MetPro: 1.393 ± 0.345
0.819MetGln: 0.819 ± 0.242
1.311MetArg: 1.311 ± 0.266
2.213MetSer: 2.213 ± 0.343
2.131MetThr: 2.131 ± 0.379
2.049MetVal: 2.049 ± 0.3
0.328MetTrp: 0.328 ± 0.138
0.41MetTyr: 0.41 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.688AsnAla: 3.688 ± 0.457
0.164AsnCys: 0.164 ± 0.101
1.967AsnAsp: 1.967 ± 0.449
2.295AsnGlu: 2.295 ± 0.429
2.295AsnPhe: 2.295 ± 0.277
3.77AsnGly: 3.77 ± 0.555
0.901AsnHis: 0.901 ± 0.312
1.639AsnIle: 1.639 ± 0.437
1.639AsnLys: 1.639 ± 0.288
4.179AsnLeu: 4.179 ± 0.444
1.147AsnMet: 1.147 ± 0.3
1.065AsnAsn: 1.065 ± 0.337
2.786AsnPro: 2.786 ± 0.468
0.983AsnGln: 0.983 ± 0.206
2.458AsnArg: 2.458 ± 0.488
0.819AsnSer: 0.819 ± 0.334
2.458AsnThr: 2.458 ± 0.478
2.868AsnVal: 2.868 ± 0.442
0.246AsnTrp: 0.246 ± 0.158
1.557AsnTyr: 1.557 ± 0.352
0.0AsnXaa: 0.0 ± 0.0
Pro
2.868ProAla: 2.868 ± 0.45
0.246ProCys: 0.246 ± 0.147
3.933ProAsp: 3.933 ± 0.429
3.688ProGlu: 3.688 ± 0.505
1.475ProPhe: 1.475 ± 0.291
2.54ProGly: 2.54 ± 0.396
0.901ProHis: 0.901 ± 0.228
1.393ProIle: 1.393 ± 0.347
2.622ProLys: 2.622 ± 0.436
2.295ProLeu: 2.295 ± 0.442
0.901ProMet: 0.901 ± 0.245
2.295ProAsn: 2.295 ± 0.398
1.393ProPro: 1.393 ± 0.359
1.393ProGln: 1.393 ± 0.262
2.131ProArg: 2.131 ± 0.339
2.213ProSer: 2.213 ± 0.424
2.213ProThr: 2.213 ± 0.404
3.196ProVal: 3.196 ± 0.563
0.574ProTrp: 0.574 ± 0.206
0.901ProTyr: 0.901 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
5.736GlnAla: 5.736 ± 1.151
0.082GlnCys: 0.082 ± 0.079
2.376GlnAsp: 2.376 ± 0.485
2.213GlnGlu: 2.213 ± 0.385
2.131GlnPhe: 2.131 ± 0.485
3.524GlnGly: 3.524 ± 0.582
0.41GlnHis: 0.41 ± 0.168
2.213GlnIle: 2.213 ± 0.285
1.967GlnLys: 1.967 ± 0.523
3.933GlnLeu: 3.933 ± 0.41
1.557GlnMet: 1.557 ± 0.352
1.065GlnAsn: 1.065 ± 0.239
0.901GlnPro: 0.901 ± 0.246
1.803GlnGln: 1.803 ± 0.3
2.376GlnArg: 2.376 ± 0.46
1.803GlnSer: 1.803 ± 0.317
1.557GlnThr: 1.557 ± 0.382
2.786GlnVal: 2.786 ± 0.381
0.656GlnTrp: 0.656 ± 0.231
1.639GlnTyr: 1.639 ± 0.415
0.0GlnXaa: 0.0 ± 0.0
Arg
6.064ArgAla: 6.064 ± 0.866
0.574ArgCys: 0.574 ± 0.252
3.442ArgAsp: 3.442 ± 0.551
4.343ArgGlu: 4.343 ± 0.672
1.639ArgPhe: 1.639 ± 0.339
5.245ArgGly: 5.245 ± 0.494
1.475ArgHis: 1.475 ± 0.353
2.868ArgIle: 2.868 ± 0.306
3.196ArgLys: 3.196 ± 0.484
4.835ArgLeu: 4.835 ± 0.588
1.721ArgMet: 1.721 ± 0.473
3.278ArgAsn: 3.278 ± 0.544
2.458ArgPro: 2.458 ± 0.415
3.36ArgGln: 3.36 ± 0.491
3.442ArgArg: 3.442 ± 0.424
3.278ArgSer: 3.278 ± 0.49
2.376ArgThr: 2.376 ± 0.328
3.606ArgVal: 3.606 ± 0.498
0.656ArgTrp: 0.656 ± 0.181
1.885ArgTyr: 1.885 ± 0.3
0.0ArgXaa: 0.0 ± 0.0
Ser
5.327SerAla: 5.327 ± 0.899
0.574SerCys: 0.574 ± 0.184
3.442SerAsp: 3.442 ± 0.422
2.786SerGlu: 2.786 ± 0.385
2.213SerPhe: 2.213 ± 0.327
5.245SerGly: 5.245 ± 0.634
1.311SerHis: 1.311 ± 0.342
2.54SerIle: 2.54 ± 0.371
3.196SerLys: 3.196 ± 0.506
4.507SerLeu: 4.507 ± 0.63
1.475SerMet: 1.475 ± 0.35
2.131SerAsn: 2.131 ± 0.496
1.967SerPro: 1.967 ± 0.381
1.803SerGln: 1.803 ± 0.367
3.114SerArg: 3.114 ± 0.422
2.622SerSer: 2.622 ± 0.428
2.376SerThr: 2.376 ± 0.448
3.688SerVal: 3.688 ± 0.503
0.901SerTrp: 0.901 ± 0.204
1.967SerTyr: 1.967 ± 0.375
0.0SerXaa: 0.0 ± 0.0
Thr
4.999ThrAla: 4.999 ± 0.535
0.492ThrCys: 0.492 ± 0.211
3.032ThrAsp: 3.032 ± 0.57
4.835ThrGlu: 4.835 ± 0.615
1.475ThrPhe: 1.475 ± 0.464
5.163ThrGly: 5.163 ± 0.841
1.229ThrHis: 1.229 ± 0.24
3.278ThrIle: 3.278 ± 0.396
4.343ThrLys: 4.343 ± 0.56
5.409ThrLeu: 5.409 ± 0.607
1.147ThrMet: 1.147 ± 0.249
2.131ThrAsn: 2.131 ± 0.4
3.032ThrPro: 3.032 ± 0.568
2.458ThrGln: 2.458 ± 0.411
2.458ThrArg: 2.458 ± 0.583
2.54ThrSer: 2.54 ± 0.52
3.442ThrThr: 3.442 ± 0.654
3.688ThrVal: 3.688 ± 0.553
0.41ThrTrp: 0.41 ± 0.166
1.229ThrTyr: 1.229 ± 0.246
0.0ThrXaa: 0.0 ± 0.0
Val
6.966ValAla: 6.966 ± 0.941
0.41ValCys: 0.41 ± 0.175
3.442ValAsp: 3.442 ± 0.525
4.507ValGlu: 4.507 ± 0.731
1.639ValPhe: 1.639 ± 0.299
4.835ValGly: 4.835 ± 0.567
1.475ValHis: 1.475 ± 0.323
2.95ValIle: 2.95 ± 0.446
4.507ValLys: 4.507 ± 0.732
4.589ValLeu: 4.589 ± 0.569
1.475ValMet: 1.475 ± 0.331
2.704ValAsn: 2.704 ± 0.577
2.458ValPro: 2.458 ± 0.377
2.95ValGln: 2.95 ± 0.368
4.671ValArg: 4.671 ± 0.71
3.933ValSer: 3.933 ± 0.54
5.409ValThr: 5.409 ± 0.598
4.507ValVal: 4.507 ± 0.723
1.311ValTrp: 1.311 ± 0.382
1.967ValTyr: 1.967 ± 0.431
0.0ValXaa: 0.0 ± 0.0
Trp
1.557TrpAla: 1.557 ± 0.37
0.164TrpCys: 0.164 ± 0.117
0.819TrpAsp: 0.819 ± 0.232
0.574TrpGlu: 0.574 ± 0.198
0.492TrpPhe: 0.492 ± 0.215
0.983TrpGly: 0.983 ± 0.239
0.656TrpHis: 0.656 ± 0.229
0.574TrpIle: 0.574 ± 0.235
1.311TrpLys: 1.311 ± 0.373
1.721TrpLeu: 1.721 ± 0.397
0.492TrpMet: 0.492 ± 0.181
0.574TrpAsn: 0.574 ± 0.304
0.41TrpPro: 0.41 ± 0.168
0.574TrpGln: 0.574 ± 0.195
0.492TrpArg: 0.492 ± 0.183
1.065TrpSer: 1.065 ± 0.363
0.656TrpThr: 0.656 ± 0.235
1.311TrpVal: 1.311 ± 0.302
0.082TrpTrp: 0.082 ± 0.084
0.164TrpTyr: 0.164 ± 0.103
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.114TyrAla: 3.114 ± 0.606
0.492TyrCys: 0.492 ± 0.225
1.967TyrAsp: 1.967 ± 0.366
2.131TyrGlu: 2.131 ± 0.457
0.574TyrPhe: 0.574 ± 0.213
2.622TyrGly: 2.622 ± 0.32
0.492TyrHis: 0.492 ± 0.178
1.065TyrIle: 1.065 ± 0.246
1.475TyrLys: 1.475 ± 0.307
1.885TyrLeu: 1.885 ± 0.362
1.393TyrMet: 1.393 ± 0.317
0.983TyrAsn: 0.983 ± 0.321
0.738TyrPro: 0.738 ± 0.238
1.311TyrGln: 1.311 ± 0.38
2.213TyrArg: 2.213 ± 0.284
1.147TyrSer: 1.147 ± 0.282
2.295TyrThr: 2.295 ± 0.457
2.131TyrVal: 2.131 ± 0.409
0.738TyrTrp: 0.738 ± 0.219
0.328TyrTyr: 0.328 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (12204 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski