Gordonia phage Easley

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8UN46|A0A2U8UN46_9CAUD Uncharacterized protein OS=Gordonia phage Easley OX=2182395 GN=8 PE=4 SV=1
MM1 pKa = 6.48VTASNVADD9 pKa = 3.86FHH11 pKa = 7.12DD12 pKa = 4.51AGEE15 pKa = 4.53GSFWWHH21 pKa = 6.98DD22 pKa = 3.58PAADD26 pKa = 3.97EE27 pKa = 4.16YY28 pKa = 11.21LITGTFDD35 pKa = 3.42PPRR38 pKa = 11.84EE39 pKa = 3.97DD40 pKa = 4.37AVYY43 pKa = 10.95LMPWDD48 pKa = 4.42GAWFAQWDD56 pKa = 4.32RR57 pKa = 11.84DD58 pKa = 3.59WEE60 pKa = 4.04AAAAQLNSIIEE71 pKa = 4.21RR72 pKa = 11.84RR73 pKa = 11.84QEE75 pKa = 3.84TAA77 pKa = 3.28

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8UMX1|A0A2U8UMX1_9CAUD Uncharacterized protein OS=Gordonia phage Easley OX=2182395 GN=63 PE=4 SV=1
MM1 pKa = 7.37TLRR4 pKa = 11.84RR5 pKa = 11.84CTRR8 pKa = 11.84PGCEE12 pKa = 3.41KK13 pKa = 10.45RR14 pKa = 11.84PRR16 pKa = 11.84SRR18 pKa = 11.84GLCHH22 pKa = 5.62THH24 pKa = 6.78YY25 pKa = 9.14MDD27 pKa = 6.13HH28 pKa = 6.62ITRR31 pKa = 11.84QKK33 pKa = 11.18AYY35 pKa = 10.13GRR37 pKa = 11.84WEE39 pKa = 4.01SSLVDD44 pKa = 3.54AEE46 pKa = 4.16PTRR49 pKa = 11.84QHH51 pKa = 5.98VEE53 pKa = 3.61RR54 pKa = 11.84LRR56 pKa = 11.84AAGVGTRR63 pKa = 11.84KK64 pKa = 9.59ISEE67 pKa = 4.23TAGVTRR73 pKa = 11.84SVLQSLIHH81 pKa = 5.91GKK83 pKa = 7.21TRR85 pKa = 11.84NGHH88 pKa = 6.0RR89 pKa = 11.84EE90 pKa = 4.03PPSKK94 pKa = 9.88TIAKK98 pKa = 9.91RR99 pKa = 11.84IADD102 pKa = 4.63KK103 pKa = 10.77ILAVPVPAQPLRR115 pKa = 11.84NGDD118 pKa = 3.62VPALGTRR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84LQALIAHH134 pKa = 7.62GYY136 pKa = 7.86SQAEE140 pKa = 3.93INRR143 pKa = 11.84RR144 pKa = 11.84FGLAADD150 pKa = 4.15NLAEE154 pKa = 5.02LIRR157 pKa = 11.84GEE159 pKa = 4.17RR160 pKa = 11.84NYY162 pKa = 10.51VDD164 pKa = 4.02PLTEE168 pKa = 3.8RR169 pKa = 11.84RR170 pKa = 11.84VTALFRR176 pKa = 11.84EE177 pKa = 4.54LQLIPGPCTRR187 pKa = 11.84ARR189 pKa = 11.84NRR191 pKa = 11.84GKK193 pKa = 10.63KK194 pKa = 9.85YY195 pKa = 10.42GWPLPLDD202 pKa = 3.51WDD204 pKa = 4.19EE205 pKa = 5.62DD206 pKa = 4.45HH207 pKa = 7.42IDD209 pKa = 3.91DD210 pKa = 5.31PNYY213 pKa = 9.03QAEE216 pKa = 4.18RR217 pKa = 11.84SHH219 pKa = 5.94VRR221 pKa = 11.84SIVRR225 pKa = 11.84DD226 pKa = 3.49PEE228 pKa = 4.53RR229 pKa = 11.84IEE231 pKa = 3.58WRR233 pKa = 11.84KK234 pKa = 9.75QKK236 pKa = 10.48VAEE239 pKa = 4.18LTALGYY245 pKa = 9.82SAEE248 pKa = 4.89DD249 pKa = 2.74IAARR253 pKa = 11.84LRR255 pKa = 11.84CTARR259 pKa = 11.84TVQRR263 pKa = 11.84DD264 pKa = 3.38RR265 pKa = 11.84SDD267 pKa = 3.43LGLVDD272 pKa = 3.71QQVASS277 pKa = 3.79

Molecular weight: 31.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

15310

32

2139

198.8

21.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.992 ± 0.59

0.927 ± 0.182

6.61 ± 0.364

5.924 ± 0.222

2.476 ± 0.155

8.432 ± 0.416

2.149 ± 0.208

4.546 ± 0.206

3.325 ± 0.208

8.021 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.16

2.978 ± 0.211

5.996 ± 0.315

3.762 ± 0.311

7.485 ± 0.406

5.153 ± 0.23

7.048 ± 0.267

6.95 ± 0.199

1.907 ± 0.15

2.175 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski