Amino acid dipepetide frequency for Vibrio phage phiVa1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.219AlaAla: 3.219 ± 0.416
0.426AlaCys: 0.426 ± 0.136
3.246AlaAsp: 3.246 ± 0.3
3.272AlaGlu: 3.272 ± 0.317
2.474AlaPhe: 2.474 ± 0.279
2.474AlaGly: 2.474 ± 0.286
0.745AlaHis: 0.745 ± 0.113
3.485AlaIle: 3.485 ± 0.315
3.751AlaLys: 3.751 ± 0.316
4.895AlaLeu: 4.895 ± 0.403
1.304AlaMet: 1.304 ± 0.216
3.219AlaAsn: 3.219 ± 0.373
1.304AlaPro: 1.304 ± 0.168
1.836AlaGln: 1.836 ± 0.257
1.889AlaArg: 1.889 ± 0.231
3.485AlaSer: 3.485 ± 0.34
3.033AlaThr: 3.033 ± 0.32
3.086AlaVal: 3.086 ± 0.304
0.372AlaTrp: 0.372 ± 0.092
2.208AlaTyr: 2.208 ± 0.214
0.0AlaXaa: 0.0 ± 0.0
Cys
0.372CysAla: 0.372 ± 0.099
0.08CysCys: 0.08 ± 0.052
0.639CysAsp: 0.639 ± 0.144
0.958CysGlu: 0.958 ± 0.195
0.479CysPhe: 0.479 ± 0.107
0.851CysGly: 0.851 ± 0.127
0.266CysHis: 0.266 ± 0.093
0.506CysIle: 0.506 ± 0.104
0.905CysLys: 0.905 ± 0.141
0.825CysLeu: 0.825 ± 0.157
0.186CysMet: 0.186 ± 0.068
0.532CysAsn: 0.532 ± 0.142
0.319CysPro: 0.319 ± 0.096
0.319CysGln: 0.319 ± 0.087
0.612CysArg: 0.612 ± 0.146
0.692CysSer: 0.692 ± 0.15
0.905CysThr: 0.905 ± 0.165
0.559CysVal: 0.559 ± 0.137
0.053CysTrp: 0.053 ± 0.038
0.133CysTyr: 0.133 ± 0.053
0.0CysXaa: 0.0 ± 0.0
Asp
3.139AspAla: 3.139 ± 0.318
0.585AspCys: 0.585 ± 0.11
4.629AspAsp: 4.629 ± 0.291
5.827AspGlu: 5.827 ± 0.384
4.071AspPhe: 4.071 ± 0.302
4.017AspGly: 4.017 ± 0.379
1.038AspHis: 1.038 ± 0.186
5.587AspIle: 5.587 ± 0.462
5.135AspLys: 5.135 ± 0.367
5.906AspLeu: 5.906 ± 0.398
1.862AspMet: 1.862 ± 0.267
3.911AspAsn: 3.911 ± 0.334
2.128AspPro: 2.128 ± 0.235
1.676AspGln: 1.676 ± 0.245
1.995AspArg: 1.995 ± 0.24
4.496AspSer: 4.496 ± 0.371
3.139AspThr: 3.139 ± 0.335
4.683AspVal: 4.683 ± 0.338
0.878AspTrp: 0.878 ± 0.215
3.672AspTyr: 3.672 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
3.406GluAla: 3.406 ± 0.34
0.559GluCys: 0.559 ± 0.11
5.055GluAsp: 5.055 ± 0.31
5.401GluGlu: 5.401 ± 0.43
3.991GluPhe: 3.991 ± 0.317
3.086GluGly: 3.086 ± 0.288
1.729GluHis: 1.729 ± 0.24
5.773GluIle: 5.773 ± 0.433
5.853GluLys: 5.853 ± 0.483
6.811GluLeu: 6.811 ± 0.427
2.501GluMet: 2.501 ± 0.339
4.922GluAsn: 4.922 ± 0.396
1.729GluPro: 1.729 ± 0.221
2.953GluGln: 2.953 ± 0.308
4.017GluArg: 4.017 ± 0.318
5.028GluSer: 5.028 ± 0.44
3.964GluThr: 3.964 ± 0.326
5.241GluVal: 5.241 ± 0.377
1.011GluTrp: 1.011 ± 0.138
3.618GluTyr: 3.618 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.261PheAla: 2.261 ± 0.226
0.692PheCys: 0.692 ± 0.141
3.991PheAsp: 3.991 ± 0.31
3.964PheGlu: 3.964 ± 0.345
2.235PhePhe: 2.235 ± 0.249
2.794PheGly: 2.794 ± 0.291
0.585PheHis: 0.585 ± 0.108
3.326PheIle: 3.326 ± 0.328
3.858PheLys: 3.858 ± 0.317
3.246PheLeu: 3.246 ± 0.29
1.117PheMet: 1.117 ± 0.201
3.379PheAsn: 3.379 ± 0.314
1.171PhePro: 1.171 ± 0.186
1.437PheGln: 1.437 ± 0.203
1.916PheArg: 1.916 ± 0.212
3.751PheSer: 3.751 ± 0.299
2.927PheThr: 2.927 ± 0.293
2.661PheVal: 2.661 ± 0.283
0.506PheTrp: 0.506 ± 0.107
1.995PheTyr: 1.995 ± 0.246
0.0PheXaa: 0.0 ± 0.0
Gly
2.607GlyAla: 2.607 ± 0.276
0.692GlyCys: 0.692 ± 0.147
3.166GlyAsp: 3.166 ± 0.374
3.911GlyGlu: 3.911 ± 0.362
2.847GlyPhe: 2.847 ± 0.297
3.592GlyGly: 3.592 ± 0.367
1.144GlyHis: 1.144 ± 0.206
3.618GlyIle: 3.618 ± 0.271
4.869GlyLys: 4.869 ± 0.31
4.496GlyLeu: 4.496 ± 0.349
1.197GlyMet: 1.197 ± 0.174
3.379GlyAsn: 3.379 ± 0.38
0.984GlyPro: 0.984 ± 0.149
2.607GlyGln: 2.607 ± 0.306
2.288GlyArg: 2.288 ± 0.248
4.257GlySer: 4.257 ± 0.366
4.071GlyThr: 4.071 ± 0.4
4.044GlyVal: 4.044 ± 0.381
0.585GlyTrp: 0.585 ± 0.133
2.98GlyTyr: 2.98 ± 0.247
0.0GlyXaa: 0.0 ± 0.0
His
0.585HisAla: 0.585 ± 0.095
0.346HisCys: 0.346 ± 0.105
0.984HisAsp: 0.984 ± 0.173
1.437HisGlu: 1.437 ± 0.2
1.304HisPhe: 1.304 ± 0.208
1.011HisGly: 1.011 ± 0.175
0.532HisHis: 0.532 ± 0.11
1.517HisIle: 1.517 ± 0.236
1.543HisLys: 1.543 ± 0.21
1.65HisLeu: 1.65 ± 0.181
0.532HisMet: 0.532 ± 0.101
1.357HisAsn: 1.357 ± 0.167
0.931HisPro: 0.931 ± 0.155
0.665HisGln: 0.665 ± 0.131
0.559HisArg: 0.559 ± 0.122
1.038HisSer: 1.038 ± 0.208
0.958HisThr: 0.958 ± 0.171
0.958HisVal: 0.958 ± 0.157
0.213HisTrp: 0.213 ± 0.071
1.038HisTyr: 1.038 ± 0.154
0.0HisXaa: 0.0 ± 0.0
Ile
3.539IleAla: 3.539 ± 0.415
0.745IleCys: 0.745 ± 0.134
5.933IleAsp: 5.933 ± 0.401
6.119IleGlu: 6.119 ± 0.43
2.927IlePhe: 2.927 ± 0.326
4.55IleGly: 4.55 ± 0.326
1.676IleHis: 1.676 ± 0.231
4.603IleIle: 4.603 ± 0.388
6.944IleLys: 6.944 ± 0.515
5.188IleLeu: 5.188 ± 0.387
1.783IleMet: 1.783 ± 0.201
5.374IleAsn: 5.374 ± 0.324
2.528IlePro: 2.528 ± 0.29
2.767IleGln: 2.767 ± 0.299
2.474IleArg: 2.474 ± 0.257
4.762IleSer: 4.762 ± 0.351
3.485IleThr: 3.485 ± 0.302
4.097IleVal: 4.097 ± 0.367
0.692IleTrp: 0.692 ± 0.131
2.661IleTyr: 2.661 ± 0.259
0.0IleXaa: 0.0 ± 0.0
Lys
4.204LysAla: 4.204 ± 0.39
0.798LysCys: 0.798 ± 0.162
5.215LysAsp: 5.215 ± 0.413
7.024LysGlu: 7.024 ± 0.591
4.177LysPhe: 4.177 ± 0.36
3.938LysGly: 3.938 ± 0.287
1.942LysHis: 1.942 ± 0.233
6.066LysIle: 6.066 ± 0.386
6.385LysLys: 6.385 ± 0.564
6.678LysLeu: 6.678 ± 0.453
2.74LysMet: 2.74 ± 0.279
5.055LysAsn: 5.055 ± 0.33
2.9LysPro: 2.9 ± 0.306
2.714LysGln: 2.714 ± 0.282
3.618LysArg: 3.618 ± 0.314
5.481LysSer: 5.481 ± 0.43
4.257LysThr: 4.257 ± 0.379
4.789LysVal: 4.789 ± 0.434
1.011LysTrp: 1.011 ± 0.158
4.443LysTyr: 4.443 ± 0.365
0.0LysXaa: 0.0 ± 0.0
Leu
3.805LeuAla: 3.805 ± 0.299
0.718LeuCys: 0.718 ± 0.125
6.439LeuAsp: 6.439 ± 0.408
5.986LeuGlu: 5.986 ± 0.463
3.539LeuPhe: 3.539 ± 0.256
4.337LeuGly: 4.337 ± 0.356
1.517LeuHis: 1.517 ± 0.204
5.295LeuIle: 5.295 ± 0.389
7.104LeuLys: 7.104 ± 0.437
5.72LeuLeu: 5.72 ± 0.388
2.208LeuMet: 2.208 ± 0.24
5.428LeuAsn: 5.428 ± 0.306
2.714LeuPro: 2.714 ± 0.222
3.459LeuGln: 3.459 ± 0.304
2.794LeuArg: 2.794 ± 0.262
6.279LeuSer: 6.279 ± 0.349
4.257LeuThr: 4.257 ± 0.354
4.337LeuVal: 4.337 ± 0.347
0.692LeuTrp: 0.692 ± 0.133
2.634LeuTyr: 2.634 ± 0.265
0.0LeuXaa: 0.0 ± 0.0
Met
1.756MetAla: 1.756 ± 0.176
0.319MetCys: 0.319 ± 0.087
1.304MetAsp: 1.304 ± 0.182
1.729MetGlu: 1.729 ± 0.225
1.064MetPhe: 1.064 ± 0.164
1.836MetGly: 1.836 ± 0.257
0.426MetHis: 0.426 ± 0.106
2.208MetIle: 2.208 ± 0.268
3.193MetLys: 3.193 ± 0.287
1.969MetLeu: 1.969 ± 0.233
0.506MetMet: 0.506 ± 0.125
1.756MetAsn: 1.756 ± 0.233
0.506MetPro: 0.506 ± 0.127
0.692MetGln: 0.692 ± 0.13
0.825MetArg: 0.825 ± 0.168
2.182MetSer: 2.182 ± 0.246
1.57MetThr: 1.57 ± 0.179
1.357MetVal: 1.357 ± 0.184
0.106MetTrp: 0.106 ± 0.054
1.011MetTyr: 1.011 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.272AsnAla: 3.272 ± 0.39
0.559AsnCys: 0.559 ± 0.119
3.539AsnAsp: 3.539 ± 0.312
5.028AsnGlu: 5.028 ± 0.366
2.847AsnPhe: 2.847 ± 0.272
4.15AsnGly: 4.15 ± 0.412
0.878AsnHis: 0.878 ± 0.136
5.667AsnIle: 5.667 ± 0.41
5.534AsnLys: 5.534 ± 0.391
5.215AsnLeu: 5.215 ± 0.34
1.304AsnMet: 1.304 ± 0.17
3.884AsnAsn: 3.884 ± 0.379
2.74AsnPro: 2.74 ± 0.251
2.607AsnGln: 2.607 ± 0.272
2.421AsnArg: 2.421 ± 0.234
3.512AsnSer: 3.512 ± 0.279
3.565AsnThr: 3.565 ± 0.408
3.672AsnVal: 3.672 ± 0.3
0.479AsnTrp: 0.479 ± 0.112
2.9AsnTyr: 2.9 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
1.49ProAla: 1.49 ± 0.199
0.346ProCys: 0.346 ± 0.09
2.421ProAsp: 2.421 ± 0.26
2.82ProGlu: 2.82 ± 0.254
1.304ProPhe: 1.304 ± 0.179
2.102ProGly: 2.102 ± 0.22
0.851ProHis: 0.851 ± 0.137
1.836ProIle: 1.836 ± 0.228
2.474ProLys: 2.474 ± 0.265
1.942ProLeu: 1.942 ± 0.227
0.851ProMet: 0.851 ± 0.194
2.395ProAsn: 2.395 ± 0.263
0.559ProPro: 0.559 ± 0.127
0.851ProGln: 0.851 ± 0.145
1.171ProArg: 1.171 ± 0.178
2.82ProSer: 2.82 ± 0.29
1.517ProThr: 1.517 ± 0.19
1.543ProVal: 1.543 ± 0.157
0.266ProTrp: 0.266 ± 0.073
1.543ProTyr: 1.543 ± 0.222
0.0ProXaa: 0.0 ± 0.0
Gln
1.916GlnAla: 1.916 ± 0.241
0.399GlnCys: 0.399 ± 0.092
2.235GlnAsp: 2.235 ± 0.269
3.246GlnGlu: 3.246 ± 0.326
1.729GlnPhe: 1.729 ± 0.225
1.995GlnGly: 1.995 ± 0.263
0.585GlnHis: 0.585 ± 0.141
3.166GlnIle: 3.166 ± 0.291
3.006GlnLys: 3.006 ± 0.366
2.634GlnLeu: 2.634 ± 0.278
1.383GlnMet: 1.383 ± 0.18
2.128GlnAsn: 2.128 ± 0.206
1.144GlnPro: 1.144 ± 0.215
1.011GlnGln: 1.011 ± 0.184
1.676GlnArg: 1.676 ± 0.217
2.581GlnSer: 2.581 ± 0.29
1.783GlnThr: 1.783 ± 0.213
1.942GlnVal: 1.942 ± 0.221
0.293GlnTrp: 0.293 ± 0.108
2.075GlnTyr: 2.075 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
1.809ArgAla: 1.809 ± 0.261
0.426ArgCys: 0.426 ± 0.105
2.581ArgAsp: 2.581 ± 0.27
2.82ArgGlu: 2.82 ± 0.266
1.783ArgPhe: 1.783 ± 0.193
2.368ArgGly: 2.368 ± 0.243
0.639ArgHis: 0.639 ± 0.141
2.9ArgIle: 2.9 ± 0.275
3.831ArgLys: 3.831 ± 0.31
2.953ArgLeu: 2.953 ± 0.325
1.171ArgMet: 1.171 ± 0.188
2.554ArgAsn: 2.554 ± 0.245
1.091ArgPro: 1.091 ± 0.146
1.383ArgGln: 1.383 ± 0.193
1.171ArgArg: 1.171 ± 0.179
2.528ArgSer: 2.528 ± 0.253
1.862ArgThr: 1.862 ± 0.232
2.714ArgVal: 2.714 ± 0.277
0.293ArgTrp: 0.293 ± 0.082
1.65ArgTyr: 1.65 ± 0.235
0.0ArgXaa: 0.0 ± 0.0
Ser
3.698SerAla: 3.698 ± 0.379
0.825SerCys: 0.825 ± 0.181
4.842SerAsp: 4.842 ± 0.382
5.8SerGlu: 5.8 ± 0.376
2.82SerPhe: 2.82 ± 0.273
4.656SerGly: 4.656 ± 0.442
1.437SerHis: 1.437 ± 0.169
5.507SerIle: 5.507 ± 0.411
5.108SerLys: 5.108 ± 0.366
5.348SerLeu: 5.348 ± 0.382
1.65SerMet: 1.65 ± 0.19
3.618SerAsn: 3.618 ± 0.329
1.995SerPro: 1.995 ± 0.219
2.474SerGln: 2.474 ± 0.238
2.395SerArg: 2.395 ± 0.317
4.417SerSer: 4.417 ± 0.412
4.443SerThr: 4.443 ± 0.355
4.39SerVal: 4.39 ± 0.3
0.905SerTrp: 0.905 ± 0.191
2.661SerTyr: 2.661 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
2.687ThrAla: 2.687 ± 0.323
0.372ThrCys: 0.372 ± 0.085
4.017ThrAsp: 4.017 ± 0.376
3.645ThrGlu: 3.645 ± 0.264
2.714ThrPhe: 2.714 ± 0.302
3.352ThrGly: 3.352 ± 0.332
1.011ThrHis: 1.011 ± 0.166
3.485ThrIle: 3.485 ± 0.298
4.337ThrLys: 4.337 ± 0.36
4.284ThrLeu: 4.284 ± 0.354
1.091ThrMet: 1.091 ± 0.186
3.326ThrAsn: 3.326 ± 0.41
2.395ThrPro: 2.395 ± 0.277
2.128ThrGln: 2.128 ± 0.276
2.208ThrArg: 2.208 ± 0.258
4.044ThrSer: 4.044 ± 0.372
2.794ThrThr: 2.794 ± 0.416
3.831ThrVal: 3.831 ± 0.37
0.692ThrTrp: 0.692 ± 0.137
2.288ThrTyr: 2.288 ± 0.251
0.0ThrXaa: 0.0 ± 0.0
Val
3.033ValAla: 3.033 ± 0.33
0.745ValCys: 0.745 ± 0.132
4.071ValAsp: 4.071 ± 0.362
4.603ValGlu: 4.603 ± 0.453
2.528ValPhe: 2.528 ± 0.251
3.539ValGly: 3.539 ± 0.281
0.984ValHis: 0.984 ± 0.174
4.257ValIle: 4.257 ± 0.378
5.002ValLys: 5.002 ± 0.381
4.762ValLeu: 4.762 ± 0.345
1.25ValMet: 1.25 ± 0.185
3.698ValAsn: 3.698 ± 0.305
1.889ValPro: 1.889 ± 0.213
3.166ValGln: 3.166 ± 0.32
2.128ValArg: 2.128 ± 0.242
4.177ValSer: 4.177 ± 0.364
3.272ValThr: 3.272 ± 0.297
3.805ValVal: 3.805 ± 0.316
0.612ValTrp: 0.612 ± 0.131
2.714ValTyr: 2.714 ± 0.236
0.0ValXaa: 0.0 ± 0.0
Trp
0.532TrpAla: 0.532 ± 0.117
0.16TrpCys: 0.16 ± 0.065
0.772TrpAsp: 0.772 ± 0.15
0.612TrpGlu: 0.612 ± 0.117
0.532TrpPhe: 0.532 ± 0.11
0.399TrpGly: 0.399 ± 0.114
0.16TrpHis: 0.16 ± 0.055
0.772TrpIle: 0.772 ± 0.138
0.825TrpLys: 0.825 ± 0.121
0.905TrpLeu: 0.905 ± 0.15
0.585TrpMet: 0.585 ± 0.138
0.878TrpAsn: 0.878 ± 0.16
0.213TrpPro: 0.213 ± 0.076
0.266TrpGln: 0.266 ± 0.081
0.372TrpArg: 0.372 ± 0.095
0.718TrpSer: 0.718 ± 0.154
0.346TrpThr: 0.346 ± 0.096
0.426TrpVal: 0.426 ± 0.112
0.133TrpTrp: 0.133 ± 0.058
0.532TrpTyr: 0.532 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.395TyrAla: 2.395 ± 0.282
0.399TyrCys: 0.399 ± 0.101
3.299TyrAsp: 3.299 ± 0.29
2.448TyrGlu: 2.448 ± 0.245
2.448TyrPhe: 2.448 ± 0.273
2.288TyrGly: 2.288 ± 0.229
1.011TyrHis: 1.011 ± 0.159
3.272TyrIle: 3.272 ± 0.32
3.778TyrLys: 3.778 ± 0.273
3.831TyrLeu: 3.831 ± 0.333
1.064TyrMet: 1.064 ± 0.166
2.98TyrAsn: 2.98 ± 0.288
1.862TyrPro: 1.862 ± 0.21
1.916TyrGln: 1.916 ± 0.223
1.995TyrArg: 1.995 ± 0.203
2.767TyrSer: 2.767 ± 0.282
2.554TyrThr: 2.554 ± 0.272
2.102TyrVal: 2.102 ± 0.244
0.346TyrTrp: 0.346 ± 0.088
1.756TyrTyr: 1.756 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 135 proteins (37587 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski