Amino acid dipepetide frequency for Streptococcus sp. X16XC17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.818AlaAla: 6.818 ± 0.169
0.486AlaCys: 0.486 ± 0.036
4.606AlaAsp: 4.606 ± 0.116
5.617AlaGlu: 5.617 ± 0.129
3.324AlaPhe: 3.324 ± 0.102
5.912AlaGly: 5.912 ± 0.129
1.409AlaHis: 1.409 ± 0.058
6.269AlaIle: 6.269 ± 0.133
5.128AlaLys: 5.128 ± 0.111
7.756AlaLeu: 7.756 ± 0.142
2.054AlaMet: 2.054 ± 0.067
3.077AlaAsn: 3.077 ± 0.102
2.276AlaPro: 2.276 ± 0.089
3.468AlaGln: 3.468 ± 0.118
3.207AlaArg: 3.207 ± 0.09
4.782AlaSer: 4.782 ± 0.109
4.735AlaThr: 4.735 ± 0.132
5.602AlaVal: 5.602 ± 0.132
0.564AlaTrp: 0.564 ± 0.032
2.75AlaTyr: 2.75 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
0.335CysAla: 0.335 ± 0.032
0.059CysCys: 0.059 ± 0.012
0.281CysAsp: 0.281 ± 0.028
0.22CysGlu: 0.22 ± 0.024
0.242CysPhe: 0.242 ± 0.024
0.491CysGly: 0.491 ± 0.039
0.186CysHis: 0.186 ± 0.023
0.278CysIle: 0.278 ± 0.024
0.186CysLys: 0.186 ± 0.022
0.606CysLeu: 0.606 ± 0.039
0.134CysMet: 0.134 ± 0.018
0.166CysAsn: 0.166 ± 0.021
0.242CysPro: 0.242 ± 0.024
0.315CysGln: 0.315 ± 0.027
0.225CysArg: 0.225 ± 0.026
0.3CysSer: 0.3 ± 0.027
0.203CysThr: 0.203 ± 0.022
0.266CysVal: 0.266 ± 0.029
0.044CysTrp: 0.044 ± 0.01
0.198CysTyr: 0.198 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.186AspAla: 4.186 ± 0.123
0.308AspCys: 0.308 ± 0.031
2.835AspAsp: 2.835 ± 0.082
4.081AspGlu: 4.081 ± 0.111
3.285AspPhe: 3.285 ± 0.089
3.983AspGly: 3.983 ± 0.117
1.043AspHis: 1.043 ± 0.054
4.191AspIle: 4.191 ± 0.091
3.888AspLys: 3.888 ± 0.108
6.135AspLeu: 6.135 ± 0.134
1.619AspMet: 1.619 ± 0.06
2.086AspAsn: 2.086 ± 0.077
1.69AspPro: 1.69 ± 0.062
2.489AspGln: 2.489 ± 0.083
2.171AspArg: 2.171 ± 0.079
3.16AspSer: 3.16 ± 0.081
2.97AspThr: 2.97 ± 0.086
4.059AspVal: 4.059 ± 0.113
0.593AspTrp: 0.593 ± 0.039
2.598AspTyr: 2.598 ± 0.089
0.0AspXaa: 0.0 ± 0.0
Glu
5.846GluAla: 5.846 ± 0.133
0.252GluCys: 0.252 ± 0.026
3.881GluAsp: 3.881 ± 0.1
6.415GluGlu: 6.415 ± 0.152
2.755GluPhe: 2.755 ± 0.087
3.951GluGly: 3.951 ± 0.114
1.421GluHis: 1.421 ± 0.067
5.458GluIle: 5.458 ± 0.129
6.157GluLys: 6.157 ± 0.131
6.987GluLeu: 6.987 ± 0.153
1.985GluMet: 1.985 ± 0.081
4.054GluAsn: 4.054 ± 0.104
1.673GluPro: 1.673 ± 0.07
2.777GluGln: 2.777 ± 0.091
3.409GluArg: 3.409 ± 0.111
3.282GluSer: 3.282 ± 0.104
3.973GluThr: 3.973 ± 0.093
5.077GluVal: 5.077 ± 0.119
0.569GluTrp: 0.569 ± 0.04
2.027GluTyr: 2.027 ± 0.072
0.0GluXaa: 0.0 ± 0.0
Phe
3.397PheAla: 3.397 ± 0.094
0.274PheCys: 0.274 ± 0.026
2.911PheAsp: 2.911 ± 0.09
3.128PheGlu: 3.128 ± 0.087
2.103PhePhe: 2.103 ± 0.095
3.407PheGly: 3.407 ± 0.106
0.872PheHis: 0.872 ± 0.047
2.999PheIle: 2.999 ± 0.104
2.252PheLys: 2.252 ± 0.072
4.657PheLeu: 4.657 ± 0.147
1.138PheMet: 1.138 ± 0.056
1.675PheAsn: 1.675 ± 0.058
1.495PhePro: 1.495 ± 0.066
1.644PheGln: 1.644 ± 0.07
1.546PheArg: 1.546 ± 0.061
3.26PheSer: 3.26 ± 0.094
2.491PheThr: 2.491 ± 0.08
3.16PheVal: 3.16 ± 0.09
0.379PheTrp: 0.379 ± 0.034
1.788PheTyr: 1.788 ± 0.074
0.0PheXaa: 0.0 ± 0.0
Gly
4.76GlyAla: 4.76 ± 0.133
0.344GlyCys: 0.344 ± 0.028
3.468GlyAsp: 3.468 ± 0.099
3.551GlyGlu: 3.551 ± 0.107
3.307GlyPhe: 3.307 ± 0.102
4.469GlyGly: 4.469 ± 0.129
1.473GlyHis: 1.473 ± 0.064
5.407GlyIle: 5.407 ± 0.127
4.508GlyLys: 4.508 ± 0.104
6.757GlyLeu: 6.757 ± 0.158
1.927GlyMet: 1.927 ± 0.074
2.782GlyAsn: 2.782 ± 0.083
1.536GlyPro: 1.536 ± 0.067
3.409GlyGln: 3.409 ± 0.098
3.043GlyArg: 3.043 ± 0.106
3.683GlySer: 3.683 ± 0.102
3.861GlyThr: 3.861 ± 0.094
4.979GlyVal: 4.979 ± 0.119
0.654GlyTrp: 0.654 ± 0.038
2.674GlyTyr: 2.674 ± 0.085
0.0GlyXaa: 0.0 ± 0.0
His
1.368HisAla: 1.368 ± 0.065
0.134HisCys: 0.134 ± 0.019
1.035HisAsp: 1.035 ± 0.052
1.231HisGlu: 1.231 ± 0.051
1.138HisPhe: 1.138 ± 0.052
1.307HisGly: 1.307 ± 0.055
0.613HisHis: 0.613 ± 0.043
1.372HisIle: 1.372 ± 0.061
0.992HisLys: 0.992 ± 0.044
2.305HisLeu: 2.305 ± 0.078
0.493HisMet: 0.493 ± 0.039
0.684HisAsn: 0.684 ± 0.044
1.013HisPro: 1.013 ± 0.051
0.943HisGln: 0.943 ± 0.05
0.933HisArg: 0.933 ± 0.051
1.109HisSer: 1.109 ± 0.061
0.999HisThr: 0.999 ± 0.053
1.333HisVal: 1.333 ± 0.057
0.134HisTrp: 0.134 ± 0.018
0.874HisTyr: 0.874 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
6.393IleAla: 6.393 ± 0.133
0.523IleCys: 0.523 ± 0.035
4.242IleAsp: 4.242 ± 0.11
5.036IleGlu: 5.036 ± 0.117
3.497IlePhe: 3.497 ± 0.105
5.07IleGly: 5.07 ± 0.14
1.302IleHis: 1.302 ± 0.056
4.818IleIle: 4.818 ± 0.128
4.132IleLys: 4.132 ± 0.102
7.644IleLeu: 7.644 ± 0.185
1.668IleMet: 1.668 ± 0.077
2.874IleAsn: 2.874 ± 0.088
2.904IlePro: 2.904 ± 0.09
2.657IleGln: 2.657 ± 0.073
2.984IleArg: 2.984 ± 0.082
4.901IleSer: 4.901 ± 0.11
3.741IleThr: 3.741 ± 0.106
4.987IleVal: 4.987 ± 0.108
0.596IleTrp: 0.596 ± 0.041
2.498IleTyr: 2.498 ± 0.074
0.0IleXaa: 0.0 ± 0.0
Lys
5.072LysAla: 5.072 ± 0.117
0.205LysCys: 0.205 ± 0.02
3.673LysAsp: 3.673 ± 0.109
6.069LysGlu: 6.069 ± 0.116
2.02LysPhe: 2.02 ± 0.069
3.702LysGly: 3.702 ± 0.093
1.18LysHis: 1.18 ± 0.049
4.735LysIle: 4.735 ± 0.108
5.158LysLys: 5.158 ± 0.131
5.233LysLeu: 5.233 ± 0.107
2.23LysMet: 2.23 ± 0.073
3.265LysAsn: 3.265 ± 0.087
1.829LysPro: 1.829 ± 0.064
2.476LysGln: 2.476 ± 0.093
2.933LysArg: 2.933 ± 0.096
3.487LysSer: 3.487 ± 0.103
3.729LysThr: 3.729 ± 0.089
4.32LysVal: 4.32 ± 0.112
0.645LysTrp: 0.645 ± 0.044
1.998LysTyr: 1.998 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
9.17LeuAla: 9.17 ± 0.183
0.444LeuCys: 0.444 ± 0.034
5.92LeuAsp: 5.92 ± 0.114
7.383LeuGlu: 7.383 ± 0.157
4.286LeuPhe: 4.286 ± 0.13
6.55LeuGly: 6.55 ± 0.147
1.836LeuHis: 1.836 ± 0.083
6.498LeuIle: 6.498 ± 0.143
5.91LeuLys: 5.91 ± 0.114
9.778LeuLeu: 9.778 ± 0.222
2.408LeuMet: 2.408 ± 0.087
3.815LeuAsn: 3.815 ± 0.099
4.015LeuPro: 4.015 ± 0.113
3.712LeuGln: 3.712 ± 0.098
3.881LeuArg: 3.881 ± 0.105
6.897LeuSer: 6.897 ± 0.15
6.418LeuThr: 6.418 ± 0.133
7.041LeuVal: 7.041 ± 0.145
0.676LeuTrp: 0.676 ± 0.039
3.07LeuTyr: 3.07 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
2.318MetAla: 2.318 ± 0.078
0.112MetCys: 0.112 ± 0.016
1.692MetAsp: 1.692 ± 0.074
1.753MetGlu: 1.753 ± 0.062
0.794MetPhe: 0.794 ± 0.048
1.758MetGly: 1.758 ± 0.072
0.376MetHis: 0.376 ± 0.033
2.015MetIle: 2.015 ± 0.077
2.025MetLys: 2.025 ± 0.085
2.247MetLeu: 2.247 ± 0.068
0.779MetMet: 0.779 ± 0.044
1.199MetAsn: 1.199 ± 0.053
0.877MetPro: 0.877 ± 0.047
0.952MetGln: 0.952 ± 0.05
1.099MetArg: 1.099 ± 0.049
1.587MetSer: 1.587 ± 0.063
2.081MetThr: 2.081 ± 0.073
1.905MetVal: 1.905 ± 0.068
0.144MetTrp: 0.144 ± 0.023
0.593MetTyr: 0.593 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
2.852AsnAla: 2.852 ± 0.08
0.222AsnCys: 0.222 ± 0.026
2.183AsnAsp: 2.183 ± 0.072
2.532AsnGlu: 2.532 ± 0.08
1.907AsnPhe: 1.907 ± 0.067
3.312AsnGly: 3.312 ± 0.11
1.006AsnHis: 1.006 ± 0.05
2.984AsnIle: 2.984 ± 0.082
2.481AsnLys: 2.481 ± 0.077
4.308AsnLeu: 4.308 ± 0.111
1.138AsnMet: 1.138 ± 0.055
1.663AsnAsn: 1.663 ± 0.069
2.2AsnPro: 2.2 ± 0.065
2.205AsnGln: 2.205 ± 0.079
2.044AsnArg: 2.044 ± 0.079
2.296AsnSer: 2.296 ± 0.084
1.868AsnThr: 1.868 ± 0.08
2.823AsnVal: 2.823 ± 0.092
0.43AsnTrp: 0.43 ± 0.032
1.653AsnTyr: 1.653 ± 0.073
0.0AsnXaa: 0.0 ± 0.0
Pro
2.699ProAla: 2.699 ± 0.09
0.129ProCys: 0.129 ± 0.018
2.108ProAsp: 2.108 ± 0.077
2.85ProGlu: 2.85 ± 0.083
1.531ProPhe: 1.531 ± 0.059
1.617ProGly: 1.617 ± 0.064
0.738ProHis: 0.738 ± 0.04
2.437ProIle: 2.437 ± 0.067
1.858ProLys: 1.858 ± 0.077
3.055ProLeu: 3.055 ± 0.085
0.757ProMet: 0.757 ± 0.048
1.648ProAsn: 1.648 ± 0.07
0.598ProPro: 0.598 ± 0.062
1.429ProGln: 1.429 ± 0.053
1.097ProArg: 1.097 ± 0.056
2.159ProSer: 2.159 ± 0.082
2.239ProThr: 2.239 ± 0.075
2.713ProVal: 2.713 ± 0.086
0.252ProTrp: 0.252 ± 0.02
1.282ProTyr: 1.282 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
3.827GlnAla: 3.827 ± 0.091
0.093GlnCys: 0.093 ± 0.016
2.188GlnAsp: 2.188 ± 0.08
3.671GlnGlu: 3.671 ± 0.103
1.746GlnPhe: 1.746 ± 0.056
2.293GlnGly: 2.293 ± 0.074
0.867GlnHis: 0.867 ± 0.051
3.089GlnIle: 3.089 ± 0.085
2.869GlnLys: 2.869 ± 0.096
4.283GlnLeu: 4.283 ± 0.106
1.136GlnMet: 1.136 ± 0.053
1.636GlnAsn: 1.636 ± 0.071
1.216GlnPro: 1.216 ± 0.059
1.69GlnGln: 1.69 ± 0.073
1.35GlnArg: 1.35 ± 0.057
2.305GlnSer: 2.305 ± 0.093
2.545GlnThr: 2.545 ± 0.068
3.573GlnVal: 3.573 ± 0.106
0.3GlnTrp: 0.3 ± 0.033
1.221GlnTyr: 1.221 ± 0.05
0.0GlnXaa: 0.0 ± 0.0
Arg
2.867ArgAla: 2.867 ± 0.092
0.188ArgCys: 0.188 ± 0.024
2.335ArgAsp: 2.335 ± 0.078
3.072ArgGlu: 3.072 ± 0.103
1.993ArgPhe: 1.993 ± 0.077
2.457ArgGly: 2.457 ± 0.079
0.95ArgHis: 0.95 ± 0.05
2.906ArgIle: 2.906 ± 0.078
2.862ArgLys: 2.862 ± 0.089
4.325ArgLeu: 4.325 ± 0.132
1.133ArgMet: 1.133 ± 0.047
1.827ArgAsn: 1.827 ± 0.073
1.39ArgPro: 1.39 ± 0.062
2.042ArgGln: 2.042 ± 0.08
2.0ArgArg: 2.0 ± 0.08
2.039ArgSer: 2.039 ± 0.087
2.137ArgThr: 2.137 ± 0.071
2.999ArgVal: 2.999 ± 0.09
0.266ArgTrp: 0.266 ± 0.025
1.585ArgTyr: 1.585 ± 0.067
0.0ArgXaa: 0.0 ± 0.0
Ser
4.032SerAla: 4.032 ± 0.112
0.313SerCys: 0.313 ± 0.035
3.368SerAsp: 3.368 ± 0.099
3.58SerGlu: 3.58 ± 0.101
2.833SerPhe: 2.833 ± 0.095
4.472SerGly: 4.472 ± 0.114
1.243SerHis: 1.243 ± 0.054
4.274SerIle: 4.274 ± 0.109
3.732SerLys: 3.732 ± 0.099
6.142SerLeu: 6.142 ± 0.134
1.495SerMet: 1.495 ± 0.054
2.398SerAsn: 2.398 ± 0.077
1.885SerPro: 1.885 ± 0.07
3.038SerGln: 3.038 ± 0.09
2.584SerArg: 2.584 ± 0.085
4.113SerSer: 4.113 ± 0.146
3.382SerThr: 3.382 ± 0.097
4.059SerVal: 4.059 ± 0.093
0.562SerTrp: 0.562 ± 0.041
2.388SerTyr: 2.388 ± 0.089
0.0SerXaa: 0.0 ± 0.0
Thr
4.569ThrAla: 4.569 ± 0.121
0.283ThrCys: 0.283 ± 0.028
3.58ThrAsp: 3.58 ± 0.103
3.71ThrGlu: 3.71 ± 0.104
2.633ThrPhe: 2.633 ± 0.081
4.266ThrGly: 4.266 ± 0.09
1.116ThrHis: 1.116 ± 0.053
4.638ThrIle: 4.638 ± 0.125
3.319ThrLys: 3.319 ± 0.096
5.693ThrLeu: 5.693 ± 0.123
1.36ThrMet: 1.36 ± 0.05
2.42ThrAsn: 2.42 ± 0.09
2.427ThrPro: 2.427 ± 0.108
1.876ThrGln: 1.876 ± 0.081
2.086ThrArg: 2.086 ± 0.069
3.558ThrSer: 3.558 ± 0.108
3.346ThrThr: 3.346 ± 0.115
4.664ThrVal: 4.664 ± 0.127
0.452ThrTrp: 0.452 ± 0.029
2.173ThrTyr: 2.173 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
6.337ValAla: 6.337 ± 0.143
0.369ValCys: 0.369 ± 0.035
4.376ValAsp: 4.376 ± 0.109
5.353ValGlu: 5.353 ± 0.131
2.984ValPhe: 2.984 ± 0.088
4.672ValGly: 4.672 ± 0.118
1.38ValHis: 1.38 ± 0.067
5.146ValIle: 5.146 ± 0.116
4.051ValLys: 4.051 ± 0.12
6.921ValLeu: 6.921 ± 0.156
1.773ValMet: 1.773 ± 0.057
2.879ValAsn: 2.879 ± 0.087
2.52ValPro: 2.52 ± 0.086
2.423ValGln: 2.423 ± 0.079
2.833ValArg: 2.833 ± 0.085
4.34ValSer: 4.34 ± 0.108
4.909ValThr: 4.909 ± 0.127
5.47ValVal: 5.47 ± 0.132
0.532ValTrp: 0.532 ± 0.042
2.464ValTyr: 2.464 ± 0.084
0.0ValXaa: 0.0 ± 0.0
Trp
0.493TrpAla: 0.493 ± 0.037
0.049TrpCys: 0.049 ± 0.012
0.469TrpAsp: 0.469 ± 0.036
0.474TrpGlu: 0.474 ± 0.029
0.379TrpPhe: 0.379 ± 0.032
0.513TrpGly: 0.513 ± 0.038
0.144TrpHis: 0.144 ± 0.02
0.642TrpIle: 0.642 ± 0.041
0.498TrpLys: 0.498 ± 0.035
0.952TrpLeu: 0.952 ± 0.055
0.269TrpMet: 0.269 ± 0.028
0.449TrpAsn: 0.449 ± 0.033
0.151TrpPro: 0.151 ± 0.021
0.425TrpGln: 0.425 ± 0.033
0.317TrpArg: 0.317 ± 0.029
0.554TrpSer: 0.554 ± 0.039
0.574TrpThr: 0.574 ± 0.035
0.442TrpVal: 0.442 ± 0.037
0.12TrpTrp: 0.12 ± 0.017
0.322TrpTyr: 0.322 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.501TyrAla: 2.501 ± 0.082
0.188TyrCys: 0.188 ± 0.023
2.217TyrAsp: 2.217 ± 0.079
2.225TyrGlu: 2.225 ± 0.081
1.819TyrPhe: 1.819 ± 0.065
2.391TyrGly: 2.391 ± 0.08
0.857TyrHis: 0.857 ± 0.049
2.31TyrIle: 2.31 ± 0.072
1.954TyrLys: 1.954 ± 0.081
3.922TyrLeu: 3.922 ± 0.097
0.808TyrMet: 0.808 ± 0.04
1.514TyrAsn: 1.514 ± 0.077
1.258TyrPro: 1.258 ± 0.058
1.932TyrGln: 1.932 ± 0.08
1.631TyrArg: 1.631 ± 0.069
2.078TyrSer: 2.078 ± 0.077
1.968TyrThr: 1.968 ± 0.073
2.259TyrVal: 2.259 ± 0.079
0.325TyrTrp: 0.325 ± 0.027
1.487TyrTyr: 1.487 ± 0.073
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1412 proteins (409481 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski