Amino acid dipepetide frequency for Gordonia phage Twinkle

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.622AlaAla: 17.622 ± 1.621
1.084AlaCys: 1.084 ± 0.293
8.326AlaAsp: 8.326 ± 0.599
8.041AlaGlu: 8.041 ± 0.786
3.593AlaPhe: 3.593 ± 0.532
9.809AlaGly: 9.809 ± 0.713
2.11AlaHis: 2.11 ± 0.435
5.532AlaIle: 5.532 ± 0.593
4.334AlaLys: 4.334 ± 0.54
9.182AlaLeu: 9.182 ± 0.538
3.707AlaMet: 3.707 ± 0.532
3.365AlaAsn: 3.365 ± 0.463
4.448AlaPro: 4.448 ± 0.505
3.593AlaGln: 3.593 ± 0.748
7.699AlaArg: 7.699 ± 0.696
5.988AlaSer: 5.988 ± 0.733
7.471AlaThr: 7.471 ± 0.637
7.528AlaVal: 7.528 ± 1.101
1.825AlaTrp: 1.825 ± 0.379
2.851AlaTyr: 2.851 ± 0.263
0.0AlaXaa: 0.0 ± 0.0
Cys
0.912CysAla: 0.912 ± 0.19
0.171CysCys: 0.171 ± 0.101
0.798CysAsp: 0.798 ± 0.248
0.513CysGlu: 0.513 ± 0.193
0.171CysPhe: 0.171 ± 0.096
1.54CysGly: 1.54 ± 0.386
0.456CysHis: 0.456 ± 0.218
0.228CysIle: 0.228 ± 0.127
0.228CysLys: 0.228 ± 0.118
0.342CysLeu: 0.342 ± 0.151
0.057CysMet: 0.057 ± 0.06
0.342CysAsn: 0.342 ± 0.146
0.513CysPro: 0.513 ± 0.222
0.285CysGln: 0.285 ± 0.164
0.684CysArg: 0.684 ± 0.235
0.456CysSer: 0.456 ± 0.168
0.456CysThr: 0.456 ± 0.24
0.627CysVal: 0.627 ± 0.186
0.171CysTrp: 0.171 ± 0.108
0.285CysTyr: 0.285 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
7.528AspAla: 7.528 ± 0.579
0.741AspCys: 0.741 ± 0.203
6.33AspAsp: 6.33 ± 0.751
5.19AspGlu: 5.19 ± 0.718
1.825AspPhe: 1.825 ± 0.348
5.076AspGly: 5.076 ± 0.603
2.167AspHis: 2.167 ± 0.421
2.509AspIle: 2.509 ± 0.413
1.882AspLys: 1.882 ± 0.32
7.015AspLeu: 7.015 ± 0.701
1.027AspMet: 1.027 ± 0.261
1.654AspAsn: 1.654 ± 0.29
5.304AspPro: 5.304 ± 0.578
2.509AspGln: 2.509 ± 0.337
4.049AspArg: 4.049 ± 0.586
2.566AspSer: 2.566 ± 0.404
3.764AspThr: 3.764 ± 0.377
4.79AspVal: 4.79 ± 0.512
1.084AspTrp: 1.084 ± 0.206
1.255AspTyr: 1.255 ± 0.239
0.0AspXaa: 0.0 ± 0.0
Glu
6.9GluAla: 6.9 ± 0.853
0.855GluCys: 0.855 ± 0.258
2.851GluAsp: 2.851 ± 0.46
2.794GluGlu: 2.794 ± 0.463
1.882GluPhe: 1.882 ± 0.37
2.851GluGly: 2.851 ± 0.388
1.312GluHis: 1.312 ± 0.291
2.794GluIle: 2.794 ± 0.411
1.54GluLys: 1.54 ± 0.303
6.33GluLeu: 6.33 ± 0.626
1.255GluMet: 1.255 ± 0.263
1.54GluAsn: 1.54 ± 0.315
2.509GluPro: 2.509 ± 0.42
2.908GluGln: 2.908 ± 0.441
4.962GluArg: 4.962 ± 0.595
3.878GluSer: 3.878 ± 0.535
3.08GluThr: 3.08 ± 0.479
4.676GluVal: 4.676 ± 0.583
1.426GluTrp: 1.426 ± 0.233
1.825GluTyr: 1.825 ± 0.387
0.0GluXaa: 0.0 ± 0.0
Phe
2.452PheAla: 2.452 ± 0.404
0.114PheCys: 0.114 ± 0.066
2.395PheAsp: 2.395 ± 0.33
1.426PheGlu: 1.426 ± 0.232
0.456PhePhe: 0.456 ± 0.22
2.338PheGly: 2.338 ± 0.463
0.57PheHis: 0.57 ± 0.239
0.855PheIle: 0.855 ± 0.222
0.57PheLys: 0.57 ± 0.186
2.167PheLeu: 2.167 ± 0.531
0.399PheMet: 0.399 ± 0.131
0.798PheAsn: 0.798 ± 0.223
1.027PhePro: 1.027 ± 0.253
0.741PheGln: 0.741 ± 0.192
1.54PheArg: 1.54 ± 0.312
1.426PheSer: 1.426 ± 0.24
2.053PheThr: 2.053 ± 0.334
1.996PheVal: 1.996 ± 0.374
0.684PheTrp: 0.684 ± 0.233
0.57PheTyr: 0.57 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
7.642GlyAla: 7.642 ± 0.855
0.684GlyCys: 0.684 ± 0.269
5.76GlyAsp: 5.76 ± 0.538
4.106GlyGlu: 4.106 ± 0.384
1.939GlyPhe: 1.939 ± 0.361
7.699GlyGly: 7.699 ± 0.824
1.825GlyHis: 1.825 ± 0.36
3.764GlyIle: 3.764 ± 0.408
3.935GlyLys: 3.935 ± 0.358
7.129GlyLeu: 7.129 ± 0.628
2.224GlyMet: 2.224 ± 0.314
2.737GlyAsn: 2.737 ± 0.452
3.707GlyPro: 3.707 ± 0.488
2.965GlyGln: 2.965 ± 0.527
6.33GlyArg: 6.33 ± 0.687
4.676GlySer: 4.676 ± 0.621
5.247GlyThr: 5.247 ± 0.539
6.615GlyVal: 6.615 ± 0.655
1.54GlyTrp: 1.54 ± 0.297
2.338GlyTyr: 2.338 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
1.825HisAla: 1.825 ± 0.398
0.285HisCys: 0.285 ± 0.114
1.483HisAsp: 1.483 ± 0.352
1.312HisGlu: 1.312 ± 0.289
0.399HisPhe: 0.399 ± 0.142
2.053HisGly: 2.053 ± 0.413
0.57HisHis: 0.57 ± 0.225
0.57HisIle: 0.57 ± 0.174
0.684HisLys: 0.684 ± 0.191
1.54HisLeu: 1.54 ± 0.352
0.342HisMet: 0.342 ± 0.111
0.513HisAsn: 0.513 ± 0.186
1.597HisPro: 1.597 ± 0.404
1.027HisGln: 1.027 ± 0.216
1.939HisArg: 1.939 ± 0.346
1.027HisSer: 1.027 ± 0.238
1.939HisThr: 1.939 ± 0.395
1.483HisVal: 1.483 ± 0.284
0.684HisTrp: 0.684 ± 0.181
0.342HisTyr: 0.342 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
6.273IleAla: 6.273 ± 0.585
0.285IleCys: 0.285 ± 0.136
4.448IleAsp: 4.448 ± 0.63
3.308IleGlu: 3.308 ± 0.528
0.456IlePhe: 0.456 ± 0.231
3.65IleGly: 3.65 ± 0.543
1.027IleHis: 1.027 ± 0.197
1.426IleIle: 1.426 ± 0.252
1.198IleLys: 1.198 ± 0.244
2.737IleLeu: 2.737 ± 0.432
1.027IleMet: 1.027 ± 0.234
1.483IleAsn: 1.483 ± 0.319
2.509IlePro: 2.509 ± 0.39
1.54IleGln: 1.54 ± 0.332
3.308IleArg: 3.308 ± 0.42
1.54IleSer: 1.54 ± 0.249
3.764IleThr: 3.764 ± 0.526
3.023IleVal: 3.023 ± 0.396
0.741IleTrp: 0.741 ± 0.261
1.084IleTyr: 1.084 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
4.163LysAla: 4.163 ± 0.502
0.114LysCys: 0.114 ± 0.093
1.369LysAsp: 1.369 ± 0.318
1.255LysGlu: 1.255 ± 0.272
0.798LysPhe: 0.798 ± 0.239
2.338LysGly: 2.338 ± 0.398
1.255LysHis: 1.255 ± 0.295
1.255LysIle: 1.255 ± 0.244
1.198LysLys: 1.198 ± 0.311
2.737LysLeu: 2.737 ± 0.437
0.456LysMet: 0.456 ± 0.127
1.198LysAsn: 1.198 ± 0.233
2.281LysPro: 2.281 ± 0.367
1.711LysGln: 1.711 ± 0.434
2.452LysArg: 2.452 ± 0.319
1.426LysSer: 1.426 ± 0.373
2.281LysThr: 2.281 ± 0.441
2.395LysVal: 2.395 ± 0.333
0.627LysTrp: 0.627 ± 0.177
0.741LysTyr: 0.741 ± 0.191
0.0LysXaa: 0.0 ± 0.0
Leu
10.322LeuAla: 10.322 ± 1.019
0.741LeuCys: 0.741 ± 0.26
5.361LeuAsp: 5.361 ± 0.68
4.619LeuGlu: 4.619 ± 0.559
2.509LeuPhe: 2.509 ± 0.613
6.843LeuGly: 6.843 ± 0.645
1.54LeuHis: 1.54 ± 0.339
3.365LeuIle: 3.365 ± 0.425
2.224LeuLys: 2.224 ± 0.371
5.703LeuLeu: 5.703 ± 0.574
2.167LeuMet: 2.167 ± 0.305
3.023LeuAsn: 3.023 ± 0.46
4.619LeuPro: 4.619 ± 0.633
2.224LeuGln: 2.224 ± 0.34
7.357LeuArg: 7.357 ± 0.686
4.676LeuSer: 4.676 ± 0.647
6.501LeuThr: 6.501 ± 0.692
6.102LeuVal: 6.102 ± 0.808
1.084LeuTrp: 1.084 ± 0.24
1.483LeuTyr: 1.483 ± 0.346
0.0LeuXaa: 0.0 ± 0.0
Met
3.08MetAla: 3.08 ± 0.567
0.399MetCys: 0.399 ± 0.149
0.741MetAsp: 0.741 ± 0.205
0.342MetGlu: 0.342 ± 0.134
0.399MetPhe: 0.399 ± 0.148
1.711MetGly: 1.711 ± 0.341
0.627MetHis: 0.627 ± 0.174
0.798MetIle: 0.798 ± 0.221
0.855MetLys: 0.855 ± 0.207
1.54MetLeu: 1.54 ± 0.238
0.285MetMet: 0.285 ± 0.128
0.912MetAsn: 0.912 ± 0.19
1.711MetPro: 1.711 ± 0.23
0.798MetGln: 0.798 ± 0.265
2.11MetArg: 2.11 ± 0.347
1.54MetSer: 1.54 ± 0.271
2.623MetThr: 2.623 ± 0.371
0.798MetVal: 0.798 ± 0.186
0.684MetTrp: 0.684 ± 0.207
0.228MetTyr: 0.228 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
2.68AsnAla: 2.68 ± 0.482
0.114AsnCys: 0.114 ± 0.077
2.281AsnAsp: 2.281 ± 0.446
1.54AsnGlu: 1.54 ± 0.251
0.513AsnPhe: 0.513 ± 0.205
2.737AsnGly: 2.737 ± 0.445
0.969AsnHis: 0.969 ± 0.208
1.312AsnIle: 1.312 ± 0.252
1.255AsnLys: 1.255 ± 0.287
2.566AsnLeu: 2.566 ± 0.363
0.399AsnMet: 0.399 ± 0.14
1.084AsnAsn: 1.084 ± 0.309
2.509AsnPro: 2.509 ± 0.351
0.969AsnGln: 0.969 ± 0.256
2.566AsnArg: 2.566 ± 0.369
1.882AsnSer: 1.882 ± 0.313
2.167AsnThr: 2.167 ± 0.413
1.597AsnVal: 1.597 ± 0.357
0.513AsnTrp: 0.513 ± 0.163
0.627AsnTyr: 0.627 ± 0.248
0.0AsnXaa: 0.0 ± 0.0
Pro
5.304ProAla: 5.304 ± 0.575
0.57ProCys: 0.57 ± 0.187
4.163ProAsp: 4.163 ± 0.725
4.277ProGlu: 4.277 ± 0.653
1.084ProPhe: 1.084 ± 0.34
6.045ProGly: 6.045 ± 0.628
0.57ProHis: 0.57 ± 0.156
2.11ProIle: 2.11 ± 0.381
1.54ProLys: 1.54 ± 0.297
3.878ProLeu: 3.878 ± 0.511
1.255ProMet: 1.255 ± 0.268
1.426ProAsn: 1.426 ± 0.241
2.68ProPro: 2.68 ± 0.473
1.597ProGln: 1.597 ± 0.334
3.878ProArg: 3.878 ± 0.502
3.137ProSer: 3.137 ± 0.454
4.505ProThr: 4.505 ± 0.494
4.22ProVal: 4.22 ± 0.526
1.483ProTrp: 1.483 ± 0.327
0.741ProTyr: 0.741 ± 0.202
0.0ProXaa: 0.0 ± 0.0
Gln
5.19GlnAla: 5.19 ± 0.804
0.228GlnCys: 0.228 ± 0.125
1.426GlnAsp: 1.426 ± 0.303
1.654GlnGlu: 1.654 ± 0.288
1.084GlnPhe: 1.084 ± 0.291
2.281GlnGly: 2.281 ± 0.439
0.57GlnHis: 0.57 ± 0.186
2.566GlnIle: 2.566 ± 0.414
1.255GlnLys: 1.255 ± 0.301
3.707GlnLeu: 3.707 ± 0.389
1.027GlnMet: 1.027 ± 0.265
0.684GlnAsn: 0.684 ± 0.198
1.54GlnPro: 1.54 ± 0.255
1.939GlnGln: 1.939 ± 0.343
3.65GlnArg: 3.65 ± 0.556
1.483GlnSer: 1.483 ± 0.309
1.825GlnThr: 1.825 ± 0.388
2.623GlnVal: 2.623 ± 0.359
0.855GlnTrp: 0.855 ± 0.233
0.684GlnTyr: 0.684 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
8.383ArgAla: 8.383 ± 0.782
0.684ArgCys: 0.684 ± 0.25
5.133ArgAsp: 5.133 ± 0.675
4.049ArgGlu: 4.049 ± 0.566
1.882ArgPhe: 1.882 ± 0.422
5.019ArgGly: 5.019 ± 0.511
1.312ArgHis: 1.312 ± 0.361
3.992ArgIle: 3.992 ± 0.429
2.623ArgLys: 2.623 ± 0.336
7.243ArgLeu: 7.243 ± 0.651
1.825ArgMet: 1.825 ± 0.367
2.908ArgAsn: 2.908 ± 0.399
3.251ArgPro: 3.251 ± 0.455
3.194ArgGln: 3.194 ± 0.514
6.273ArgArg: 6.273 ± 0.658
4.391ArgSer: 4.391 ± 0.46
4.79ArgThr: 4.79 ± 0.678
4.847ArgVal: 4.847 ± 0.554
2.566ArgTrp: 2.566 ± 0.442
2.053ArgTyr: 2.053 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
5.931SerAla: 5.931 ± 0.651
0.285SerCys: 0.285 ± 0.161
3.308SerAsp: 3.308 ± 0.552
2.68SerGlu: 2.68 ± 0.404
1.198SerPhe: 1.198 ± 0.299
5.988SerGly: 5.988 ± 0.705
0.912SerHis: 0.912 ± 0.249
2.737SerIle: 2.737 ± 0.344
1.54SerLys: 1.54 ± 0.302
4.505SerLeu: 4.505 ± 0.516
1.597SerMet: 1.597 ± 0.316
1.597SerAsn: 1.597 ± 0.466
2.908SerPro: 2.908 ± 0.43
2.452SerGln: 2.452 ± 0.471
2.965SerArg: 2.965 ± 0.547
3.707SerSer: 3.707 ± 0.495
3.764SerThr: 3.764 ± 0.435
4.277SerVal: 4.277 ± 0.503
1.141SerTrp: 1.141 ± 0.199
1.483SerTyr: 1.483 ± 0.396
0.0SerXaa: 0.0 ± 0.0
Thr
9.809ThrAla: 9.809 ± 0.768
0.741ThrCys: 0.741 ± 0.234
4.733ThrAsp: 4.733 ± 0.583
3.536ThrGlu: 3.536 ± 0.503
1.255ThrPhe: 1.255 ± 0.295
5.817ThrGly: 5.817 ± 0.728
1.141ThrHis: 1.141 ± 0.236
3.935ThrIle: 3.935 ± 0.48
1.711ThrLys: 1.711 ± 0.309
4.904ThrLeu: 4.904 ± 0.499
1.198ThrMet: 1.198 ± 0.227
1.825ThrAsn: 1.825 ± 0.3
4.619ThrPro: 4.619 ± 0.63
1.426ThrGln: 1.426 ± 0.281
5.475ThrArg: 5.475 ± 0.811
3.479ThrSer: 3.479 ± 0.437
5.19ThrThr: 5.19 ± 0.537
5.247ThrVal: 5.247 ± 0.567
1.426ThrTrp: 1.426 ± 0.311
1.54ThrTyr: 1.54 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
7.87ValAla: 7.87 ± 0.652
0.627ValCys: 0.627 ± 0.182
4.619ValAsp: 4.619 ± 0.481
5.304ValGlu: 5.304 ± 0.562
1.939ValPhe: 1.939 ± 0.385
5.646ValGly: 5.646 ± 0.672
1.483ValHis: 1.483 ± 0.285
3.707ValIle: 3.707 ± 0.513
2.566ValLys: 2.566 ± 0.495
5.76ValLeu: 5.76 ± 0.768
1.027ValMet: 1.027 ± 0.256
1.939ValAsn: 1.939 ± 0.345
3.707ValPro: 3.707 ± 0.482
2.509ValGln: 2.509 ± 0.428
5.133ValArg: 5.133 ± 0.398
4.847ValSer: 4.847 ± 0.567
4.847ValThr: 4.847 ± 0.528
4.79ValVal: 4.79 ± 0.585
1.084ValTrp: 1.084 ± 0.288
1.597ValTyr: 1.597 ± 0.332
0.0ValXaa: 0.0 ± 0.0
Trp
1.54TrpAla: 1.54 ± 0.295
0.342TrpCys: 0.342 ± 0.113
1.312TrpAsp: 1.312 ± 0.328
0.741TrpGlu: 0.741 ± 0.198
0.57TrpPhe: 0.57 ± 0.194
1.198TrpGly: 1.198 ± 0.269
0.684TrpHis: 0.684 ± 0.212
0.741TrpIle: 0.741 ± 0.208
0.57TrpLys: 0.57 ± 0.233
1.597TrpLeu: 1.597 ± 0.263
0.513TrpMet: 0.513 ± 0.164
0.627TrpAsn: 0.627 ± 0.177
1.597TrpPro: 1.597 ± 0.32
0.798TrpGln: 0.798 ± 0.164
2.452TrpArg: 2.452 ± 0.397
1.312TrpSer: 1.312 ± 0.297
1.54TrpThr: 1.54 ± 0.248
1.369TrpVal: 1.369 ± 0.34
0.684TrpTrp: 0.684 ± 0.18
0.456TrpTyr: 0.456 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.68TyrAla: 2.68 ± 0.37
0.171TyrCys: 0.171 ± 0.098
1.426TyrAsp: 1.426 ± 0.265
1.483TyrGlu: 1.483 ± 0.316
0.627TyrPhe: 0.627 ± 0.192
2.224TyrGly: 2.224 ± 0.392
0.513TyrHis: 0.513 ± 0.27
0.627TyrIle: 0.627 ± 0.208
0.342TyrLys: 0.342 ± 0.152
1.882TyrLeu: 1.882 ± 0.516
0.342TyrMet: 0.342 ± 0.112
0.627TyrAsn: 0.627 ± 0.205
1.426TyrPro: 1.426 ± 0.343
1.141TyrGln: 1.141 ± 0.257
1.597TyrArg: 1.597 ± 0.346
1.54TyrSer: 1.54 ± 0.268
1.198TyrThr: 1.198 ± 0.239
1.996TyrVal: 1.996 ± 0.323
0.342TyrTrp: 0.342 ± 0.174
0.399TyrTyr: 0.399 ± 0.149
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (17536 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski