Herbiconiux sp. SALV-R1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Herbiconiux; unclassified Herbiconiux

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5J2W1|A0A6M5J2W1_9MICO DUF3021 domain-containing protein OS=Herbiconiux sp. SALV-R1 OX=2735133 GN=HL652_13830 PE=4 SV=1
MM1 pKa = 7.46HH2 pKa = 7.56RR3 pKa = 11.84RR4 pKa = 11.84TTAALRR10 pKa = 11.84TTTLLAAVSLLAAGCSAGGDD30 pKa = 3.37AGSTGSASGGDD41 pKa = 3.98DD42 pKa = 4.3VEE44 pKa = 4.47LTYY47 pKa = 11.01QSAEE51 pKa = 4.29GEE53 pKa = 4.61CASGPRR59 pKa = 11.84DD60 pKa = 3.31GVDD63 pKa = 3.1YY64 pKa = 8.83DD65 pKa = 3.99TADD68 pKa = 3.42ALIKK72 pKa = 10.55SFQEE76 pKa = 3.79PSEE79 pKa = 4.3GLLQTEE85 pKa = 5.18KK86 pKa = 10.9LPQPLGADD94 pKa = 3.31TTIAFLNNDD103 pKa = 3.71TAVAGIMYY111 pKa = 10.26AAIQKK116 pKa = 9.73AAADD120 pKa = 3.65AGINLVNVSTGTDD133 pKa = 3.16AQSINSALNSVVEE146 pKa = 4.48LDD148 pKa = 3.56PDD150 pKa = 3.85AVISVAIDD158 pKa = 2.96ATFYY162 pKa = 10.94QDD164 pKa = 3.92QLKK167 pKa = 9.91QLEE170 pKa = 4.48ANGVPIVYY178 pKa = 10.22SSQPNADD185 pKa = 3.6EE186 pKa = 5.03FGLDD190 pKa = 4.3DD191 pKa = 5.34SLGGYY196 pKa = 9.49NGSLVNGKK204 pKa = 8.94VLAAGAVAFTCGTGDD219 pKa = 4.28DD220 pKa = 3.88FVFYY224 pKa = 10.47NIPEE228 pKa = 4.48LGFSAIQLEE237 pKa = 4.66STQEE241 pKa = 3.89YY242 pKa = 10.08LAEE245 pKa = 4.38LCPDD249 pKa = 3.49CNLRR253 pKa = 11.84VVDD256 pKa = 5.33ISIADD261 pKa = 3.86PSPADD266 pKa = 4.25KK267 pKa = 10.51IVSDD271 pKa = 4.09LQSHH275 pKa = 7.18PEE277 pKa = 3.39TDD279 pKa = 4.02YY280 pKa = 11.28FVTPADD286 pKa = 3.55QFQVGLADD294 pKa = 3.69KK295 pKa = 10.54AQLAGLTNAYY305 pKa = 10.38GFGQSSLPPNVQQLADD321 pKa = 3.93GLQSAGFAVDD331 pKa = 3.93LDD333 pKa = 3.57MYY335 pKa = 10.6MYY337 pKa = 9.47LTLDD341 pKa = 3.23EE342 pKa = 5.09AFRR345 pKa = 11.84KK346 pKa = 8.9IQGVYY351 pKa = 9.32QPYY354 pKa = 10.58DD355 pKa = 3.01DD356 pKa = 4.07WEE358 pKa = 4.19AVNRR362 pKa = 11.84SVSRR366 pKa = 11.84VLTPANAGEE375 pKa = 4.09YY376 pKa = 10.39LGGFVAYY383 pKa = 9.48PGMQDD388 pKa = 4.36DD389 pKa = 6.58FKK391 pKa = 10.81TLWGKK396 pKa = 10.05

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5IQK4|A0A6M5IQK4_9MICO Substrate-binding domain-containing protein OS=Herbiconiux sp. SALV-R1 OX=2735133 GN=HL652_03595 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4153

0

4153

1355947

29

2219

326.5

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.624 ± 0.053

0.489 ± 0.008

6.061 ± 0.031

5.745 ± 0.036

3.202 ± 0.024

9.216 ± 0.032

1.911 ± 0.02

4.192 ± 0.026

1.885 ± 0.025

10.143 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.609 ± 0.013

1.949 ± 0.022

5.537 ± 0.029

2.613 ± 0.021

6.964 ± 0.045

6.081 ± 0.031

6.165 ± 0.035

9.172 ± 0.039

1.446 ± 0.015

1.994 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski