Parachlamydia acanthamoebae (strain UV7)

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Parachlamydiaceae; Parachlamydia; Parachlamydia acanthamoebae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2784 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8L1R6|F8L1R6_PARAV Thiamine diphosphokinase OS=Parachlamydia acanthamoebae (strain UV7) OX=765952 GN=PUV_22740 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.38NLYY5 pKa = 9.78DD6 pKa = 3.81LPLRR10 pKa = 11.84ISLMLTVFLCSLSQLEE26 pKa = 4.57ASSCIPGRR34 pKa = 11.84KK35 pKa = 9.06GSPGAPGDD43 pKa = 4.34PGPMGPTGPXGEE55 pKa = 5.89DD56 pKa = 3.35GPAGATGPVGPTGATGPVGLPGNQKK81 pKa = 10.84LNVLPCSGQTTMIPISITLPPLGASSGSTVFFTYY115 pKa = 9.82TATPTQLTIDD125 pKa = 4.13MASSDD130 pKa = 3.77QFALVVMPGLTTLTDD145 pKa = 3.88QISHH149 pKa = 6.43TVTQTAPNTFEE160 pKa = 5.24INLSDD165 pKa = 3.56AADD168 pKa = 3.91YY169 pKa = 11.0IGIALIGCPPVDD181 pKa = 3.17

Molecular weight: 18.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8KXD1|F8KXD1_PARAV Pyruvate phosphate dikinase OS=Parachlamydia acanthamoebae (strain UV7) OX=765952 GN=ppdK PE=3 SV=2
MM1 pKa = 7.9IGIFSDD7 pKa = 3.43EE8 pKa = 3.87MLEE11 pKa = 4.07RR12 pKa = 11.84GEE14 pKa = 5.54LEE16 pKa = 3.76ACFFKK21 pKa = 11.1GVVVSVYY28 pKa = 10.8ALARR32 pKa = 11.84TGRR35 pKa = 11.84AFMLIMRR42 pKa = 11.84IXGKK46 pKa = 10.48AMYY49 pKa = 10.48NFMRR53 pKa = 11.84FLPRR57 pKa = 11.84IRR59 pKa = 11.84LDD61 pKa = 3.2LRR63 pKa = 11.84FSRR66 pKa = 11.84KK67 pKa = 7.27STT69 pKa = 3.33

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2784

0

2784

914357

13

3414

328.4

37.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.937 ± 0.051

1.284 ± 0.02

4.804 ± 0.033

6.887 ± 0.045

5.154 ± 0.041

5.851 ± 0.061

2.601 ± 0.025

7.282 ± 0.039

7.028 ± 0.051

10.785 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.017

4.363 ± 0.032

4.234 ± 0.032

4.518 ± 0.04

4.262 ± 0.033

6.679 ± 0.036

5.022 ± 0.044

5.581 ± 0.039

1.188 ± 0.019

3.32 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski