Limimaricola soesokkakensis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3683 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1X6ZMF2|A0A1X6ZMF2_9RHOB 4 4'-diaponeurosporenoate glycosyltransferase OS=Limimaricola soesokkakensis OX=1343159 GN=crtQ PE=4 SV=1
MM1 pKa = 7.88 PEE3 pKa = 4.13 LTSSLTRR10 pKa = 11.84 GASLSTAVLAAIAGFILPQVSLADD34 pKa = 3.53 SPVIQTEE41 pKa = 4.43 GPVIYY46 pKa = 10.59 LSDD49 pKa = 4.02 NLDD52 pKa = 3.41 EE53 pKa = 4.43 EE54 pKa = 5.87 ANLGWCIDD62 pKa = 3.59 TEE64 pKa = 4.57 GRR66 pKa = 11.84 GEE68 pKa = 3.99 SDD70 pKa = 2.94 QLHH73 pKa = 5.87 AHH75 pKa = 5.3 SCKK78 pKa = 9.4 PTGDD82 pKa = 3.98 DD83 pKa = 3.76 VLFSYY88 pKa = 10.73 APEE91 pKa = 4.02 SEE93 pKa = 4.57 RR94 pKa = 11.84 IEE96 pKa = 4.37 SATYY100 pKa = 10.54 AGLCMAYY107 pKa = 9.98 NAPDD111 pKa = 4.04 DD112 pKa = 4.06 EE113 pKa = 4.85 EE114 pKa = 5.37 NPFGLIACDD123 pKa = 3.74 DD124 pKa = 3.83 TDD126 pKa = 3.91 PNQRR130 pKa = 11.84 FEE132 pKa = 4.55 YY133 pKa = 9.34 DD134 pKa = 3.22 TEE136 pKa = 4.19 TGQIHH141 pKa = 7.01 LGSDD145 pKa = 3.13 ATQCVTVSAQIDD157 pKa = 3.73 DD158 pKa = 4.49 AGPFQSRR165 pKa = 11.84 DD166 pKa = 3.55 LLLAACDD173 pKa = 4.1 EE174 pKa = 4.64 LDD176 pKa = 3.87 QSFKK180 pKa = 9.99 TWTVRR185 pKa = 11.84 DD186 pKa = 3.61
Molecular weight: 20.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.872
Dawson 3.745
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.088
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.872
Patrickios 0.769
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A1X6YHN9|A0A1X6YHN9_9RHOB Inner membrane protein YebE OS=Limimaricola soesokkakensis OX=1343159 GN=yebE PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 9.25 SRR15 pKa = 11.84 HH16 pKa = 3.83 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.01 VLSAA44 pKa = 3.99
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3683
0
3683
1159187
29
5820
314.7
34.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.139 ± 0.068
0.828 ± 0.015
5.932 ± 0.041
6.545 ± 0.041
3.458 ± 0.029
9.092 ± 0.05
2.072 ± 0.023
4.859 ± 0.027
2.562 ± 0.037
10.304 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.684 ± 0.023
2.176 ± 0.026
5.362 ± 0.035
2.88 ± 0.019
7.635 ± 0.058
4.865 ± 0.028
5.112 ± 0.03
7.073 ± 0.044
1.411 ± 0.019
2.012 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here