Plum bark necrosis stem pitting-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8RS59|A8RS59_9CLOS Hydrophobic protein OS=Plum bark necrosis stem pitting-associated virus OX=675077 GN=P6 PE=4 SV=1
EEE2 pKa = 3.98YY3 pKa = 10.16EE4 pKa = 4.08YYY6 pKa = 10.99EEE8 pKa = 4.09LIPSPNRR15 pKa = 11.84LLRR18 pKa = 11.84PCRR21 pKa = 11.84SHHH24 pKa = 6.64ASIGFLLDDD33 pKa = 3.87DD34 pKa = 5.13NLSGMTLNLEEE45 pKa = 4.1CEEE48 pKa = 4.74DD49 pKa = 3.68FQTSDDD55 pKa = 3.13STAVDDD61 pKa = 3.29VVLSEEE67 pKa = 4.03KK68 pKa = 10.62DD69 pKa = 3.03AFRR72 pKa = 11.84PYYY75 pKa = 10.57AMKKK79 pKa = 8.8YY80 pKa = 9.53PLNKKK85 pKa = 9.73IKKK88 pKa = 9.54SRR90 pKa = 11.84RR91 pKa = 11.84LDDD94 pKa = 3.48TRR96 pKa = 11.84SNLYYY101 pKa = 10.37FEEE104 pKa = 3.9RR105 pKa = 11.84NLLGNVEEE113 pKa = 4.63NRR115 pKa = 11.84SLRR118 pKa = 11.84PSLVHHH124 pKa = 7.38DD125 pKa = 3.67VDDD128 pKa = 3.34DD129 pKa = 4.18FRR131 pKa = 11.84VFIDDD136 pKa = 3.38LKKK139 pKa = 9.72SEEE142 pKa = 4.8VGDDD146 pKa = 3.78IGQNVVALEEE156 pKa = 3.63WLRR159 pKa = 11.84TRR161 pKa = 11.84TPLGKKK167 pKa = 9.92ALARR171 pKa = 11.84DDD173 pKa = 4.88EEE175 pKa = 4.49SPDDD179 pKa = 3.2RR180 pKa = 11.84SDDD183 pKa = 3.31NRR185 pKa = 11.84FKKK188 pKa = 11.14MVKKK192 pKa = 10.56DDD194 pKa = 3.7KKK196 pKa = 11.19KKK198 pKa = 11.02DDD200 pKa = 3.68TAEEE204 pKa = 4.01SLPSGQNIVYYY215 pKa = 9.95HH216 pKa = 6.11RR217 pKa = 11.84NVCLLFSSVFQQLTEEE233 pKa = 3.96LKKK236 pKa = 11.1LLKKK240 pKa = 9.69HHH242 pKa = 6.13KKK244 pKa = 10.1FHHH247 pKa = 6.47CSLDDD252 pKa = 5.46DD253 pKa = 3.89AQSLRR258 pKa = 11.84DDD260 pKa = 3.66AVGDDD265 pKa = 3.48TSYYY269 pKa = 10.99YY270 pKa = 9.99AEEE273 pKa = 4.25DDD275 pKa = 3.67SKKK278 pKa = 11.1YY279 pKa = 10.61DD280 pKa = 3.37KK281 pKa = 10.82QNDDD285 pKa = 3.46TKKK288 pKa = 10.76IEEE291 pKa = 3.84HH292 pKa = 7.31EE293 pKa = 4.0YYY295 pKa = 10.22RR296 pKa = 11.84LGMDDD301 pKa = 3.61EEE303 pKa = 4.65LEEE306 pKa = 4.2YY307 pKa = 9.7CASDDD312 pKa = 5.58SSQVGSTSRR321 pKa = 11.84GFSLSIGSQRR331 pKa = 11.84RR332 pKa = 11.84TGTATTWLGNTVVNMALMARR352 pKa = 11.84VLGCEEE358 pKa = 4.34FSSMAFSGDDD368 pKa = 3.47DD369 pKa = 3.38ILFHHH374 pKa = 6.98HH375 pKa = 6.92PLDDD379 pKa = 3.82DDD381 pKa = 3.4SNYYY385 pKa = 8.4EE386 pKa = 3.61HHH388 pKa = 6.68AFDDD392 pKa = 3.64KKK394 pKa = 10.3FRR396 pKa = 11.84MTVPYYY402 pKa = 9.97CSKKK406 pKa = 10.09FVVGSSGLNFVPDDD420 pKa = 3.94VKKK423 pKa = 10.92FVSLGSEEE431 pKa = 4.17KK432 pKa = 10.82DDD434 pKa = 3.62DD435 pKa = 3.55EE436 pKa = 5.1VMRR439 pKa = 11.84EEE441 pKa = 3.76FVSFLDDD448 pKa = 3.51TKKK451 pKa = 10.47YYY453 pKa = 11.41EE454 pKa = 4.1DD455 pKa = 3.7TVCDDD460 pKa = 4.38MTTLVSVKKK469 pKa = 9.27GYYY472 pKa = 11.54DDD474 pKa = 5.73LSCSLFALHHH484 pKa = 6.83LRR486 pKa = 11.84ANFGQFMRR494 pKa = 11.84LWRR497 pKa = 11.84LCQQNVVFEEE507 pKa = 4.34KK508 pKa = 10.93EEE510 pKa = 4.05RR511 pKa = 11.84NKKK514 pKa = 10.73VVDDD518 pKa = 4.42EE519 pKa = 5.58DDD521 pKa = 3.31FGN

Molecular weight: 59.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8RS74|A8RS74_9CLOS Minor capsid protein OS=Plum bark necrosis stem pitting-associated virus OX=675077 GN=CPm PE=4 SV=1
MM1 pKa = 7.17ATADD5 pKa = 4.26RR6 pKa = 11.84QSMPATTPIDD16 pKa = 3.08EE17 pKa = 4.4WTNTEE22 pKa = 4.05FPGATFLKK30 pKa = 10.3RR31 pKa = 11.84KK32 pKa = 8.39VANDD36 pKa = 3.69LEE38 pKa = 4.47LDD40 pKa = 3.45KK41 pKa = 11.52LLTNKK46 pKa = 10.38SITEE50 pKa = 3.95DD51 pKa = 3.05QRR53 pKa = 11.84RR54 pKa = 11.84IYY56 pKa = 11.14AEE58 pKa = 3.7ARR60 pKa = 11.84VKK62 pKa = 10.55RR63 pKa = 11.84SRR65 pKa = 11.84LTLLNEE71 pKa = 4.14AEE73 pKa = 4.3KK74 pKa = 10.57EE75 pKa = 4.01AVRR78 pKa = 11.84QVEE81 pKa = 4.23AARR84 pKa = 11.84TLPGLPATPSGVKK97 pKa = 10.32NVVATMKK104 pKa = 10.74SFMNNPSEE112 pKa = 3.96TAMFDD117 pKa = 3.22WTKK120 pKa = 10.5MKK122 pKa = 10.01IPKK125 pKa = 9.07MVDD128 pKa = 2.46VRR130 pKa = 11.84TPGVVTTEE138 pKa = 4.07HH139 pKa = 6.46SIEE142 pKa = 4.27VYY144 pKa = 10.34DD145 pKa = 5.76ALRR148 pKa = 11.84LWALSKK154 pKa = 10.76GLQDD158 pKa = 3.48TDD160 pKa = 3.25EE161 pKa = 5.23DD162 pKa = 4.01MASLVTTGFQNALSFSTVKK181 pKa = 9.37TAAPAGHH188 pKa = 7.08TDD190 pKa = 3.11NKK192 pKa = 10.7LVGSSKK198 pKa = 10.65PDD200 pKa = 3.0MEE202 pKa = 5.04LPHH205 pKa = 7.88NEE207 pKa = 3.6FKK209 pKa = 11.2GVVEE213 pKa = 4.79SAIQSYY219 pKa = 10.32GYY221 pKa = 10.18EE222 pKa = 3.98NPLRR226 pKa = 11.84QWMRR230 pKa = 11.84KK231 pKa = 8.43NSAAVIQMTSAGFLQPNIKK250 pKa = 9.91KK251 pKa = 10.35LNEE254 pKa = 3.89NGIPPQYY261 pKa = 10.71YY262 pKa = 9.69PFGGDD267 pKa = 3.39FLVVDD272 pKa = 4.28SRR274 pKa = 11.84VFGYY278 pKa = 10.1SAALASQLGRR288 pKa = 11.84MVALNRR294 pKa = 11.84KK295 pKa = 9.21RR296 pKa = 11.84NGNKK300 pKa = 9.67EE301 pKa = 3.4IHH303 pKa = 6.21NLYY306 pKa = 9.62EE307 pKa = 3.89QTTAAPQIFTGGNQSGGRR325 pKa = 3.63

Molecular weight: 35.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4547

57

2343

649.6

72.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.07 ± 0.51

1.847 ± 0.269

5.652 ± 0.327

5.08 ± 0.256

4.926 ± 0.579

5.828 ± 0.361

1.803 ± 0.445

4.64 ± 0.357

6.51 ± 0.989

10.227 ± 0.607

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.111 ± 0.266

4.596 ± 0.466

4.486 ± 0.58

2.375 ± 0.311

5.234 ± 0.465

9.743 ± 0.554

5.806 ± 0.298

8.907 ± 0.518

0.946 ± 0.117

3.211 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski