Streptococcus ferus
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1812 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2X3VID1|A0A2X3VID1_9STRE Nicotinamidase/pyrazinamidase OS=Streptococcus ferus OX=1345 GN=NCTC12278_01728 PE=4 SV=1
MM1 pKa = 7.32 PTYY4 pKa = 10.54 TDD6 pKa = 3.93 GEE8 pKa = 4.27 EE9 pKa = 3.87 LQIALEE15 pKa = 4.32 EE16 pKa = 4.54 YY17 pKa = 9.44 EE18 pKa = 4.26 VSSKK22 pKa = 11.27 GEE24 pKa = 3.59 QVTIGKK30 pKa = 9.37 NDD32 pKa = 3.94 EE33 pKa = 4.17 IVIGTVTQVYY43 pKa = 10.42 DD44 pKa = 3.55 NTSGAGEE51 pKa = 3.94
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.844
IPC2_protein 3.821
IPC_protein 3.605
Toseland 3.478
ProMoST 3.719
Dawson 3.592
Bjellqvist 3.872
Wikipedia 3.503
Rodwell 3.478
Grimsley 3.401
Solomon 3.528
Lehninger 3.49
Nozaki 3.745
DTASelect 3.795
Thurlkill 3.541
EMBOSS 3.516
Sillero 3.732
Patrickios 1.799
IPC_peptide 3.541
IPC2_peptide 3.706
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A2X3W0D6|A0A2X3W0D6_9STRE Putative proton-coupled thiamine transporter YuaJ OS=Streptococcus ferus OX=1345 GN=thiT PE=4 SV=1
MM1 pKa = 7.92 PKK3 pKa = 10.14 QKK5 pKa = 7.59 THH7 pKa = 6.46 RR8 pKa = 11.84 ASAKK12 pKa = 8.58 RR13 pKa = 11.84 FKK15 pKa = 10.11 RR16 pKa = 11.84 TGSGGLKK23 pKa = 10.04 RR24 pKa = 11.84 FRR26 pKa = 11.84 AYY28 pKa = 9.4 TSHH31 pKa = 7.17 RR32 pKa = 11.84 FHH34 pKa = 7.37 GKK36 pKa = 6.22 TKK38 pKa = 9.47 KK39 pKa = 9.37 QRR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.65 LRR45 pKa = 11.84 KK46 pKa = 9.84 ASMVSAGDD54 pKa = 3.58 FKK56 pKa = 10.91 RR57 pKa = 11.84 IKK59 pKa = 11.06 SMLTRR64 pKa = 11.84 LKK66 pKa = 10.92
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 10.891
IPC_protein 12.281
Toseland 12.457
ProMoST 12.925
Dawson 12.457
Bjellqvist 12.442
Wikipedia 12.91
Rodwell 12.325
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.442
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.047
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 8.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1812
0
1812
554771
31
2197
306.2
34.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.043 ± 0.056
0.482 ± 0.015
5.936 ± 0.048
6.575 ± 0.065
4.528 ± 0.046
6.643 ± 0.056
1.897 ± 0.028
6.989 ± 0.056
6.577 ± 0.053
10.422 ± 0.082
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.388 ± 0.027
4.207 ± 0.042
3.333 ± 0.032
4.339 ± 0.037
4.109 ± 0.046
6.377 ± 0.058
5.725 ± 0.063
6.757 ± 0.049
0.886 ± 0.02
3.787 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here