Boseongicola sp. CCM32

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Boseongicola; unclassified Boseongicola

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7J8N4|A0A4P7J8N4_9RHOB Acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha OS=Boseongicola sp. CCM32 OX=2552942 GN=E2K80_01180 PE=4 SV=1
MM1 pKa = 7.53TSFLKK6 pKa = 9.95TAALTALVAAAPLIPAGQAAAEE28 pKa = 4.35GEE30 pKa = 4.3RR31 pKa = 11.84YY32 pKa = 10.27VLVSHH37 pKa = 7.06APDD40 pKa = 3.63SDD42 pKa = 3.83SWWNTIRR49 pKa = 11.84NALALAGDD57 pKa = 3.7QMDD60 pKa = 3.76VEE62 pKa = 4.65VEE64 pKa = 4.08YY65 pKa = 10.98RR66 pKa = 11.84NPPTGDD72 pKa = 3.14IADD75 pKa = 3.67MARR78 pKa = 11.84IIEE81 pKa = 4.13QVAASGPDD89 pKa = 3.89GIITTLADD97 pKa = 3.63YY98 pKa = 10.66DD99 pKa = 4.23VLSGPIQSAVDD110 pKa = 3.36AGIDD114 pKa = 3.75VIIMNSGTPEE124 pKa = 3.48QAADD128 pKa = 3.64VGALMYY134 pKa = 10.42VGQPEE139 pKa = 4.31YY140 pKa = 10.92DD141 pKa = 3.25AGLAAGQRR149 pKa = 11.84AARR152 pKa = 11.84DD153 pKa = 3.56GVTSFLCVNHH163 pKa = 6.44VISNPVVAEE172 pKa = 4.37RR173 pKa = 11.84CQGFADD179 pKa = 4.08GLGVDD184 pKa = 5.04LGDD187 pKa = 4.38SMIDD191 pKa = 3.26SGTDD195 pKa = 3.06PSEE198 pKa = 3.66IQNRR202 pKa = 11.84VLAYY206 pKa = 10.9LNANPDD212 pKa = 2.9TDD214 pKa = 5.59AILTLGPTSADD225 pKa = 3.37PTILALEE232 pKa = 4.37SNGMAGDD239 pKa = 4.0IYY241 pKa = 10.9FGTFDD246 pKa = 4.09LGDD249 pKa = 4.36NIVQGIRR256 pKa = 11.84DD257 pKa = 3.94GVIEE261 pKa = 4.31WGIDD265 pKa = 3.32QQPFLQAYY273 pKa = 8.83LPVVVLTNYY282 pKa = 9.37HH283 pKa = 6.81RR284 pKa = 11.84YY285 pKa = 9.54GVLPGNNINSGPGFITADD303 pKa = 3.47GLDD306 pKa = 4.03LVSEE310 pKa = 4.49YY311 pKa = 11.05AGEE314 pKa = 4.07YY315 pKa = 9.72RR316 pKa = 4.9

Molecular weight: 33.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7JHX4|A0A4P7JHX4_9RHOB Mechanosensitive ion channel family protein OS=Boseongicola sp. CCM32 OX=2552942 GN=E2K80_07925 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 8.83HH15 pKa = 4.64RR16 pKa = 11.84HH17 pKa = 3.91GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3500

0

3500

1077088

35

1810

307.7

33.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.253 ± 0.047

0.943 ± 0.012

6.161 ± 0.04

5.627 ± 0.036

3.775 ± 0.026

8.818 ± 0.041

2.154 ± 0.02

5.414 ± 0.029

2.665 ± 0.031

10.183 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.88 ± 0.02

2.55 ± 0.02

5.236 ± 0.027

3.274 ± 0.026

6.712 ± 0.04

5.077 ± 0.033

5.62 ± 0.025

7.017 ± 0.034

1.395 ± 0.019

2.248 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski