Streptococcus phage Javan51

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AFU1|A0A4D6AFU1_9CAUD Tail length tape-measure protein OS=Streptococcus phage Javan51 OX=2548223 GN=Javan51_0030 PE=4 SV=1
MM1 pKa = 7.48EE2 pKa = 5.02LVKK5 pKa = 10.02TIQIGDD11 pKa = 4.56DD12 pKa = 3.37IYY14 pKa = 11.61LPIPDD19 pKa = 3.64QFGIQEE25 pKa = 4.08GQEE28 pKa = 3.9FNLYY32 pKa = 9.82QSSDD36 pKa = 3.3GTLVLSPLDD45 pKa = 3.8SKK47 pKa = 11.53LSADD51 pKa = 3.94YY52 pKa = 10.88QSLSSDD58 pKa = 3.54EE59 pKa = 4.4QEE61 pKa = 4.3TTVVEE66 pKa = 4.07QATLDD71 pKa = 3.56KK72 pKa = 10.84VANSVLSRR80 pKa = 11.84HH81 pKa = 6.06LDD83 pKa = 3.29AFKK86 pKa = 10.92EE87 pKa = 4.0LAEE90 pKa = 4.13

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AFF5|A0A4D6AFF5_9CAUD Adenine-specific methyltransferase OS=Streptococcus phage Javan51 OX=2548223 GN=Javan51_0012 PE=3 SV=1
MM1 pKa = 7.52FEE3 pKa = 4.54TIFFYY8 pKa = 10.94SLIGIYY14 pKa = 10.48LFFGLYY20 pKa = 9.77LNYY23 pKa = 8.2MTIRR27 pKa = 11.84DD28 pKa = 4.0DD29 pKa = 3.09IRR31 pKa = 11.84RR32 pKa = 11.84EE33 pKa = 3.9KK34 pKa = 10.04EE35 pKa = 3.44RR36 pKa = 11.84KK37 pKa = 9.28AEE39 pKa = 4.16KK40 pKa = 9.83KK41 pKa = 9.32HH42 pKa = 6.0HH43 pKa = 6.64SNNTTPLHH51 pKa = 6.61RR52 pKa = 11.84SRR54 pKa = 5.13

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

10517

44

1039

300.5

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.504

0.713 ± 0.126

6.247 ± 0.453

7.036 ± 0.341

3.813 ± 0.196

6.998 ± 0.332

1.959 ± 0.172

5.848 ± 0.346

6.922 ± 0.269

9.385 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.188

4.383 ± 0.214

3.157 ± 0.242

4.165 ± 0.207

4.25 ± 0.351

6.818 ± 0.455

6.989 ± 0.459

6.599 ± 0.342

1.417 ± 0.104

3.699 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski