Capybara microvirus Cap3_SP_363
Average proteome isoelectric point is 5.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVS5|A0A4V1FVS5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_363 OX=2585435 PE=4 SV=1
MM1 pKa = 7.07 IRR3 pKa = 11.84 KK4 pKa = 9.49 LIGEE8 pKa = 4.41 HH9 pKa = 5.87 VPSVVQDD16 pKa = 4.52 DD17 pKa = 3.47 IRR19 pKa = 11.84 LITVVDD25 pKa = 3.58 EE26 pKa = 4.52 EE27 pKa = 5.48 GNVSSTFSKK36 pKa = 10.77 YY37 pKa = 10.45 DD38 pKa = 3.57 PSSVSYY44 pKa = 10.87 GCVGDD49 pKa = 3.57 WSLNSLISAGINPSTLNLSTNNRR72 pKa = 11.84 LNKK75 pKa = 9.71 FDD77 pKa = 4.06 EE78 pKa = 4.91 FSDD81 pKa = 4.13 FEE83 pKa = 5.15 AYY85 pKa = 10.21 ADD87 pKa = 3.73 SIMRR91 pKa = 11.84 DD92 pKa = 3.73 VIIEE96 pKa = 4.19 TPQVDD101 pKa = 3.98 SNEE104 pKa = 4.16 NNNN107 pKa = 3.37
Molecular weight: 11.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.273
IPC2_protein 4.113
IPC_protein 4.037
Toseland 3.834
ProMoST 4.19
Dawson 4.024
Bjellqvist 4.177
Wikipedia 3.948
Rodwell 3.872
Grimsley 3.757
Solomon 4.012
Lehninger 3.973
Nozaki 4.139
DTASelect 4.355
Thurlkill 3.884
EMBOSS 3.961
Sillero 4.164
Patrickios 3.656
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.08
Protein with the highest isoelectric point:
>tr|A0A4P8W5Q0|A0A4P8W5Q0_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_363 OX=2585435 PE=4 SV=1
MM1 pKa = 7.42 LTQTLNIRR9 pKa = 11.84 NNTHH13 pKa = 5.59 YY14 pKa = 10.1 FCPSGSRR21 pKa = 11.84 LINSVINPSTEE32 pKa = 3.83 RR33 pKa = 11.84 YY34 pKa = 9.34 KK35 pKa = 10.69 SLRR38 pKa = 11.84 VQGYY42 pKa = 6.98 NQRR45 pKa = 11.84 LYY47 pKa = 11.38 YY48 pKa = 10.29 EE49 pKa = 3.92 YY50 pKa = 10.05 TYY52 pKa = 10.59 CKK54 pKa = 9.95 SKK56 pKa = 10.67 NYY58 pKa = 9.93 PVFSYY63 pKa = 10.49 TLTYY67 pKa = 10.59 NNKK70 pKa = 9.25 SLPMFYY76 pKa = 11.08 GNAVHH81 pKa = 7.67 DD82 pKa = 4.59 YY83 pKa = 10.84 NDD85 pKa = 2.45 IRR87 pKa = 11.84 YY88 pKa = 8.08 FFRR91 pKa = 11.84 EE92 pKa = 4.01 SGFDD96 pKa = 3.23 KK97 pKa = 10.87 HH98 pKa = 6.69 LLRR101 pKa = 11.84 HH102 pKa = 5.64 YY103 pKa = 10.69 GSVVKK108 pKa = 9.98 YY109 pKa = 8.81 FVASEE114 pKa = 4.09 LGEE117 pKa = 4.34 GGISHH122 pKa = 6.8 QDD124 pKa = 3.29 SLSHH128 pKa = 5.82 NNKK131 pKa = 9.16 RR132 pKa = 11.84 AQRR135 pKa = 11.84 GKK137 pKa = 10.59 DD138 pKa = 3.51 NNPHH142 pKa = 4.87 YY143 pKa = 10.7 HH144 pKa = 6.35 VIFFVYY150 pKa = 9.94 NANDD154 pKa = 2.99 TRR156 pKa = 11.84 YY157 pKa = 9.71 PYY159 pKa = 10.49 IRR161 pKa = 11.84 INGKK165 pKa = 6.96 QFKK168 pKa = 9.89 TIVQRR173 pKa = 11.84 YY174 pKa = 4.38 WQGDD178 pKa = 2.72 AWRR181 pKa = 11.84 TKK183 pKa = 10.33 KK184 pKa = 9.52 PQEE187 pKa = 4.04 YY188 pKa = 10.19 KK189 pKa = 10.84 FGIAMNAKK197 pKa = 9.12 YY198 pKa = 10.65 NLGEE202 pKa = 4.1 ITNGSAIRR210 pKa = 11.84 YY211 pKa = 6.18 NSKK214 pKa = 10.86 YY215 pKa = 9.39 VVKK218 pKa = 10.65 DD219 pKa = 3.16 VLQSRR224 pKa = 11.84 KK225 pKa = 9.88 DD226 pKa = 3.7 DD227 pKa = 3.57 SCLRR231 pKa = 11.84 SCEE234 pKa = 4.36 EE235 pKa = 3.8 YY236 pKa = 9.75 WNSVDD241 pKa = 3.45 GEE243 pKa = 4.46 YY244 pKa = 10.51 FITHH248 pKa = 6.72 HH249 pKa = 6.65 KK250 pKa = 10.4 LFWDD254 pKa = 4.44 DD255 pKa = 3.57 YY256 pKa = 10.06 FDD258 pKa = 5.62 RR259 pKa = 11.84 YY260 pKa = 11.27 VNDD263 pKa = 3.63 FASDD267 pKa = 3.42 VEE269 pKa = 4.6 YY270 pKa = 10.74 INGISDD276 pKa = 3.59 VFGGFHH282 pKa = 6.62 YY283 pKa = 10.5 EE284 pKa = 3.94 VADD287 pKa = 4.13 FSGMSEE293 pKa = 4.02 NEE295 pKa = 3.91 LYY297 pKa = 10.61 DD298 pKa = 3.9 AYY300 pKa = 11.42 VYY302 pKa = 10.32 IYY304 pKa = 10.29 GDD306 pKa = 3.17 RR307 pKa = 11.84 VNFDD311 pKa = 3.45 NLLTEE316 pKa = 4.15 HH317 pKa = 7.4 RR318 pKa = 11.84 DD319 pKa = 3.65 YY320 pKa = 11.14 IIDD323 pKa = 4.37 LEE325 pKa = 4.16 INRR328 pKa = 11.84 YY329 pKa = 8.49 KK330 pKa = 10.57 NRR332 pKa = 11.84 HH333 pKa = 4.14 RR334 pKa = 11.84 TKK336 pKa = 10.52 VKK338 pKa = 9.07 ISNGIGLSALDD349 pKa = 3.87 KK350 pKa = 10.68 MVDD353 pKa = 3.59 GKK355 pKa = 10.95 IPMPVDD361 pKa = 3.34 SKK363 pKa = 10.75 KK364 pKa = 10.91 IKK366 pKa = 8.76 MQYY369 pKa = 10.41 ADD371 pKa = 2.65 IYY373 pKa = 9.29 YY374 pKa = 8.5 YY375 pKa = 10.89 RR376 pKa = 11.84 KK377 pKa = 9.28 MYY379 pKa = 10.8 YY380 pKa = 10.06 DD381 pKa = 3.48 VEE383 pKa = 4.43 KK384 pKa = 10.72 DD385 pKa = 2.99 INGNSRR391 pKa = 11.84 YY392 pKa = 9.71 VINYY396 pKa = 9.17 KK397 pKa = 10.63 GIEE400 pKa = 3.97 FRR402 pKa = 11.84 KK403 pKa = 10.3 KK404 pKa = 10.37 NLLNEE409 pKa = 4.09 LDD411 pKa = 3.68 KK412 pKa = 11.6 LQLKK416 pKa = 9.21 VRR418 pKa = 11.84 NCISLVSNSLEE429 pKa = 3.79 LQDD432 pKa = 4.61 RR433 pKa = 11.84 FKK435 pKa = 11.25 EE436 pKa = 4.23 HH437 pKa = 6.16 YY438 pKa = 9.22 LKK440 pKa = 10.77 CSRR443 pKa = 11.84 DD444 pKa = 3.76 FYY446 pKa = 11.54 FEE448 pKa = 4.94 NFDD451 pKa = 4.13 KK452 pKa = 11.33 NVVLLNFSDD461 pKa = 3.9 YY462 pKa = 11.31 LYY464 pKa = 10.69 INNIDD469 pKa = 3.86 NFIEE473 pKa = 4.4 KK474 pKa = 9.44 YY475 pKa = 10.34 AYY477 pKa = 9.63 YY478 pKa = 10.77 SKK480 pKa = 10.48 IYY482 pKa = 10.05 RR483 pKa = 11.84 NRR485 pKa = 11.84 LFRR488 pKa = 11.84 QFDD491 pKa = 4.21 DD492 pKa = 3.64 NFVPLSSYY500 pKa = 10.59 VAIDD504 pKa = 4.52 PISDD508 pKa = 3.25 YY509 pKa = 11.48 GRR511 pKa = 11.84 FIEE514 pKa = 4.85 CRR516 pKa = 11.84 VYY518 pKa = 11.14 DD519 pKa = 4.7 EE520 pKa = 6.31 DD521 pKa = 4.34 YY522 pKa = 10.99 TDD524 pKa = 4.19 CFYY527 pKa = 11.38 DD528 pKa = 3.48 RR529 pKa = 11.84 QRR531 pKa = 11.84 HH532 pKa = 5.08 HH533 pKa = 7.22 RR534 pKa = 11.84 LHH536 pKa = 7.06 LYY538 pKa = 10.5 AEE540 pKa = 4.78 YY541 pKa = 10.15 IKK543 pKa = 10.65 HH544 pKa = 6.8 PYY546 pKa = 8.26 FQEE549 pKa = 4.04 IACLCGFFDD558 pKa = 6.09 CIQDD562 pKa = 3.97 FVDD565 pKa = 3.39 KK566 pKa = 11.28 LKK568 pKa = 10.97 DD569 pKa = 3.27 EE570 pKa = 4.45 KK571 pKa = 10.66 QRR573 pKa = 11.84 KK574 pKa = 8.59 DD575 pKa = 3.13 RR576 pKa = 11.84 EE577 pKa = 3.65 EE578 pKa = 4.32 RR579 pKa = 11.84 EE580 pKa = 4.0 RR581 pKa = 11.84 VKK583 pKa = 11.23 ANAYY587 pKa = 10.31 SCGLLL592 pKa = 3.59
Molecular weight: 71.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.36
IPC2_protein 7.614
IPC_protein 7.439
Toseland 7.176
ProMoST 7.98
Dawson 8.126
Bjellqvist 8.404
Wikipedia 8.083
Rodwell 8.156
Grimsley 7.263
Solomon 8.17
Lehninger 8.185
Nozaki 8.434
DTASelect 8.214
Thurlkill 8.244
EMBOSS 8.317
Sillero 8.521
Patrickios 3.49
IPC_peptide 8.17
IPC2_peptide 7.395
IPC2.peptide.svr19 7.644
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1671
107
592
417.8
47.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.925 ± 1.683
0.898 ± 0.495
7.54 ± 0.332
4.608 ± 0.388
4.967 ± 0.419
6.344 ± 0.783
1.855 ± 0.568
5.745 ± 0.425
6.523 ± 0.544
7.241 ± 0.171
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.334 ± 0.465
7.959 ± 0.614
2.454 ± 0.317
4.189 ± 0.788
4.668 ± 1.039
7.899 ± 0.724
4.907 ± 1.12
6.104 ± 0.473
0.958 ± 0.151
6.882 ± 1.5
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here