Amino acid dipepetide frequency for Shigella phage Sfin-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.868AlaAla: 7.868 ± 0.901
1.049AlaCys: 1.049 ± 0.312
3.737AlaAsp: 3.737 ± 0.458
5.442AlaGlu: 5.442 ± 0.603
2.098AlaPhe: 2.098 ± 0.367
5.704AlaGly: 5.704 ± 0.638
1.049AlaHis: 1.049 ± 0.235
6.688AlaIle: 6.688 ± 0.694
6.753AlaLys: 6.753 ± 1.019
5.901AlaLeu: 5.901 ± 0.691
2.819AlaMet: 2.819 ± 0.384
3.016AlaAsn: 3.016 ± 0.388
1.377AlaPro: 1.377 ± 0.295
3.803AlaGln: 3.803 ± 0.591
4.131AlaArg: 4.131 ± 0.518
5.245AlaSer: 5.245 ± 0.823
4.524AlaThr: 4.524 ± 0.574
5.18AlaVal: 5.18 ± 0.55
1.18AlaTrp: 1.18 ± 0.249
2.688AlaTyr: 2.688 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.217
0.197CysCys: 0.197 ± 0.115
0.852CysAsp: 0.852 ± 0.24
0.983CysGlu: 0.983 ± 0.248
0.59CysPhe: 0.59 ± 0.209
1.246CysGly: 1.246 ± 0.327
0.459CysHis: 0.459 ± 0.204
0.983CysIle: 0.983 ± 0.264
0.852CysLys: 0.852 ± 0.238
0.852CysLeu: 0.852 ± 0.219
0.525CysMet: 0.525 ± 0.186
0.787CysAsn: 0.787 ± 0.221
0.525CysPro: 0.525 ± 0.155
0.328CysGln: 0.328 ± 0.132
0.59CysArg: 0.59 ± 0.217
0.59CysSer: 0.59 ± 0.178
0.721CysThr: 0.721 ± 0.253
1.049CysVal: 1.049 ± 0.234
0.197CysTrp: 0.197 ± 0.124
0.459CysTyr: 0.459 ± 0.183
0.0CysXaa: 0.0 ± 0.0
Asp
5.77AspAla: 5.77 ± 0.691
0.393AspCys: 0.393 ± 0.153
3.934AspAsp: 3.934 ± 0.541
4.721AspGlu: 4.721 ± 0.488
3.082AspPhe: 3.082 ± 0.471
6.884AspGly: 6.884 ± 0.87
1.18AspHis: 1.18 ± 0.295
3.541AspIle: 3.541 ± 0.442
3.868AspLys: 3.868 ± 0.459
3.475AspLeu: 3.475 ± 0.415
1.901AspMet: 1.901 ± 0.329
3.344AspAsn: 3.344 ± 0.515
1.77AspPro: 1.77 ± 0.346
1.836AspGln: 1.836 ± 0.382
2.36AspArg: 2.36 ± 0.544
4.852AspSer: 4.852 ± 0.517
2.36AspThr: 2.36 ± 0.381
4.262AspVal: 4.262 ± 0.569
0.525AspTrp: 0.525 ± 0.184
2.164AspTyr: 2.164 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
5.114GluAla: 5.114 ± 0.594
1.049GluCys: 1.049 ± 0.297
4.393GluAsp: 4.393 ± 0.515
4.721GluGlu: 4.721 ± 0.66
3.213GluPhe: 3.213 ± 0.482
2.95GluGly: 2.95 ± 0.419
1.311GluHis: 1.311 ± 0.314
6.819GluIle: 6.819 ± 0.582
4.262GluLys: 4.262 ± 0.737
4.983GluLeu: 4.983 ± 0.617
2.491GluMet: 2.491 ± 0.39
3.082GluAsn: 3.082 ± 0.399
1.77GluPro: 1.77 ± 0.458
2.623GluGln: 2.623 ± 0.531
3.082GluArg: 3.082 ± 0.596
4.065GluSer: 4.065 ± 0.488
3.803GluThr: 3.803 ± 0.591
5.442GluVal: 5.442 ± 0.608
0.721GluTrp: 0.721 ± 0.16
2.754GluTyr: 2.754 ± 0.388
0.0GluXaa: 0.0 ± 0.0
Phe
2.819PheAla: 2.819 ± 0.352
0.918PheCys: 0.918 ± 0.277
3.344PheAsp: 3.344 ± 0.441
3.213PheGlu: 3.213 ± 0.434
1.77PhePhe: 1.77 ± 0.433
3.213PheGly: 3.213 ± 0.514
0.721PheHis: 0.721 ± 0.242
2.229PheIle: 2.229 ± 0.347
3.016PheLys: 3.016 ± 0.427
1.967PheLeu: 1.967 ± 0.314
1.508PheMet: 1.508 ± 0.296
2.95PheAsn: 2.95 ± 0.379
1.574PhePro: 1.574 ± 0.301
1.246PheGln: 1.246 ± 0.305
1.836PheArg: 1.836 ± 0.341
2.295PheSer: 2.295 ± 0.366
2.557PheThr: 2.557 ± 0.441
2.426PheVal: 2.426 ± 0.343
0.852PheTrp: 0.852 ± 0.256
1.115PheTyr: 1.115 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
5.18GlyAla: 5.18 ± 0.868
1.311GlyCys: 1.311 ± 0.3
4.655GlyAsp: 4.655 ± 0.521
4.458GlyGlu: 4.458 ± 0.462
2.426GlyPhe: 2.426 ± 0.385
5.901GlyGly: 5.901 ± 0.921
1.508GlyHis: 1.508 ± 0.407
3.541GlyIle: 3.541 ± 0.467
5.835GlyLys: 5.835 ± 0.579
4.196GlyLeu: 4.196 ± 0.396
2.819GlyMet: 2.819 ± 0.518
3.737GlyAsn: 3.737 ± 0.551
0.656GlyPro: 0.656 ± 0.228
2.36GlyGln: 2.36 ± 0.359
3.475GlyArg: 3.475 ± 0.494
5.114GlySer: 5.114 ± 0.587
3.672GlyThr: 3.672 ± 0.419
5.966GlyVal: 5.966 ± 0.59
1.639GlyTrp: 1.639 ± 0.287
3.147GlyTyr: 3.147 ± 0.577
0.0GlyXaa: 0.0 ± 0.0
His
0.852HisAla: 0.852 ± 0.311
0.197HisCys: 0.197 ± 0.115
1.049HisAsp: 1.049 ± 0.251
1.705HisGlu: 1.705 ± 0.317
0.721HisPhe: 0.721 ± 0.201
1.377HisGly: 1.377 ± 0.367
0.59HisHis: 0.59 ± 0.189
1.18HisIle: 1.18 ± 0.352
1.311HisLys: 1.311 ± 0.307
1.18HisLeu: 1.18 ± 0.379
0.393HisMet: 0.393 ± 0.154
0.787HisAsn: 0.787 ± 0.299
0.787HisPro: 0.787 ± 0.273
0.59HisGln: 0.59 ± 0.159
1.049HisArg: 1.049 ± 0.316
1.377HisSer: 1.377 ± 0.32
1.311HisThr: 1.311 ± 0.27
0.983HisVal: 0.983 ± 0.338
0.262HisTrp: 0.262 ± 0.129
0.656HisTyr: 0.656 ± 0.193
0.0HisXaa: 0.0 ± 0.0
Ile
6.294IleAla: 6.294 ± 0.567
0.787IleCys: 0.787 ± 0.218
5.507IleAsp: 5.507 ± 0.528
5.245IleGlu: 5.245 ± 0.566
2.36IlePhe: 2.36 ± 0.378
4.327IleGly: 4.327 ± 0.498
0.918IleHis: 0.918 ± 0.246
3.934IleIle: 3.934 ± 0.41
5.376IleLys: 5.376 ± 0.592
3.344IleLeu: 3.344 ± 0.516
1.836IleMet: 1.836 ± 0.372
4.065IleAsn: 4.065 ± 0.498
2.36IlePro: 2.36 ± 0.4
2.295IleGln: 2.295 ± 0.342
2.623IleArg: 2.623 ± 0.383
4.524IleSer: 4.524 ± 0.575
5.049IleThr: 5.049 ± 0.459
4.196IleVal: 4.196 ± 0.523
0.656IleTrp: 0.656 ± 0.197
2.557IleTyr: 2.557 ± 0.41
0.0IleXaa: 0.0 ± 0.0
Lys
6.425LysAla: 6.425 ± 0.919
0.918LysCys: 0.918 ± 0.285
3.868LysAsp: 3.868 ± 0.495
6.098LysGlu: 6.098 ± 0.653
3.082LysPhe: 3.082 ± 0.639
4.131LysGly: 4.131 ± 0.408
1.574LysHis: 1.574 ± 0.364
4.524LysIle: 4.524 ± 0.552
4.655LysLys: 4.655 ± 0.592
4.917LysLeu: 4.917 ± 0.503
2.688LysMet: 2.688 ± 0.397
3.475LysAsn: 3.475 ± 0.491
2.885LysPro: 2.885 ± 0.518
2.754LysGln: 2.754 ± 0.494
3.672LysArg: 3.672 ± 0.425
3.672LysSer: 3.672 ± 0.474
3.606LysThr: 3.606 ± 0.494
5.114LysVal: 5.114 ± 0.636
0.59LysTrp: 0.59 ± 0.18
2.164LysTyr: 2.164 ± 0.329
0.0LysXaa: 0.0 ± 0.0
Leu
4.983LeuAla: 4.983 ± 0.788
0.852LeuCys: 0.852 ± 0.25
3.541LeuAsp: 3.541 ± 0.425
3.409LeuGlu: 3.409 ± 0.487
2.229LeuPhe: 2.229 ± 0.425
3.475LeuGly: 3.475 ± 0.534
1.311LeuHis: 1.311 ± 0.39
4.721LeuIle: 4.721 ± 0.464
4.786LeuLys: 4.786 ± 0.65
4.262LeuLeu: 4.262 ± 0.527
1.77LeuMet: 1.77 ± 0.292
2.819LeuAsn: 2.819 ± 0.427
3.278LeuPro: 3.278 ± 0.457
1.705LeuGln: 1.705 ± 0.273
3.213LeuArg: 3.213 ± 0.41
5.114LeuSer: 5.114 ± 0.536
5.245LeuThr: 5.245 ± 0.536
3.737LeuVal: 3.737 ± 0.551
1.18LeuTrp: 1.18 ± 0.248
1.901LeuTyr: 1.901 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.491MetAla: 2.491 ± 0.427
0.328MetCys: 0.328 ± 0.156
1.311MetAsp: 1.311 ± 0.329
2.688MetGlu: 2.688 ± 0.422
1.508MetPhe: 1.508 ± 0.301
0.918MetGly: 0.918 ± 0.252
0.656MetHis: 0.656 ± 0.188
2.819MetIle: 2.819 ± 0.46
2.688MetLys: 2.688 ± 0.428
2.164MetLeu: 2.164 ± 0.39
1.639MetMet: 1.639 ± 0.361
1.705MetAsn: 1.705 ± 0.302
0.983MetPro: 0.983 ± 0.253
1.115MetGln: 1.115 ± 0.297
2.098MetArg: 2.098 ± 0.383
2.623MetSer: 2.623 ± 0.394
1.442MetThr: 1.442 ± 0.266
2.033MetVal: 2.033 ± 0.273
0.459MetTrp: 0.459 ± 0.146
0.787MetTyr: 0.787 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
3.737AsnAla: 3.737 ± 0.58
0.393AsnCys: 0.393 ± 0.153
3.475AsnAsp: 3.475 ± 0.473
3.016AsnGlu: 3.016 ± 0.398
2.557AsnPhe: 2.557 ± 0.434
4.852AsnGly: 4.852 ± 0.879
0.918AsnHis: 0.918 ± 0.282
3.278AsnIle: 3.278 ± 0.387
2.95AsnLys: 2.95 ± 0.475
3.016AsnLeu: 3.016 ± 0.465
0.983AsnMet: 0.983 ± 0.25
2.819AsnAsn: 2.819 ± 0.562
2.033AsnPro: 2.033 ± 0.272
3.016AsnGln: 3.016 ± 0.475
1.508AsnArg: 1.508 ± 0.319
3.213AsnSer: 3.213 ± 0.453
2.033AsnThr: 2.033 ± 0.367
3.541AsnVal: 3.541 ± 0.486
0.983AsnTrp: 0.983 ± 0.274
1.639AsnTyr: 1.639 ± 0.255
0.0AsnXaa: 0.0 ± 0.0
Pro
2.295ProAla: 2.295 ± 0.409
0.393ProCys: 0.393 ± 0.172
2.295ProAsp: 2.295 ± 0.463
2.623ProGlu: 2.623 ± 0.48
1.18ProPhe: 1.18 ± 0.331
3.082ProGly: 3.082 ± 0.436
0.59ProHis: 0.59 ± 0.16
2.164ProIle: 2.164 ± 0.43
1.705ProLys: 1.705 ± 0.36
1.508ProLeu: 1.508 ± 0.238
0.918ProMet: 0.918 ± 0.311
2.033ProAsn: 2.033 ± 0.414
1.377ProPro: 1.377 ± 0.469
1.246ProGln: 1.246 ± 0.291
1.442ProArg: 1.442 ± 0.308
1.967ProSer: 1.967 ± 0.374
1.442ProThr: 1.442 ± 0.267
3.147ProVal: 3.147 ± 0.33
0.459ProTrp: 0.459 ± 0.195
1.574ProTyr: 1.574 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
3.606GlnAla: 3.606 ± 0.492
0.262GlnCys: 0.262 ± 0.119
1.705GlnAsp: 1.705 ± 0.395
2.426GlnGlu: 2.426 ± 0.388
1.246GlnPhe: 1.246 ± 0.306
2.229GlnGly: 2.229 ± 0.384
0.59GlnHis: 0.59 ± 0.191
3.016GlnIle: 3.016 ± 0.4
2.426GlnLys: 2.426 ± 0.417
3.475GlnLeu: 3.475 ± 0.606
1.18GlnMet: 1.18 ± 0.245
1.836GlnAsn: 1.836 ± 0.348
1.18GlnPro: 1.18 ± 0.312
2.033GlnGln: 2.033 ± 0.462
1.639GlnArg: 1.639 ± 0.35
2.426GlnSer: 2.426 ± 0.483
1.574GlnThr: 1.574 ± 0.347
2.623GlnVal: 2.623 ± 0.358
0.787GlnTrp: 0.787 ± 0.227
1.639GlnTyr: 1.639 ± 0.34
0.0GlnXaa: 0.0 ± 0.0
Arg
3.672ArgAla: 3.672 ± 0.54
0.983ArgCys: 0.983 ± 0.314
2.754ArgAsp: 2.754 ± 0.592
3.934ArgGlu: 3.934 ± 0.505
2.819ArgPhe: 2.819 ± 0.441
2.95ArgGly: 2.95 ± 0.432
0.721ArgHis: 0.721 ± 0.25
3.147ArgIle: 3.147 ± 0.532
3.672ArgLys: 3.672 ± 0.435
3.147ArgLeu: 3.147 ± 0.41
1.377ArgMet: 1.377 ± 0.296
2.164ArgAsn: 2.164 ± 0.354
1.77ArgPro: 1.77 ± 0.518
1.18ArgGln: 1.18 ± 0.274
3.016ArgArg: 3.016 ± 0.485
2.557ArgSer: 2.557 ± 0.367
1.508ArgThr: 1.508 ± 0.389
3.868ArgVal: 3.868 ± 0.465
0.656ArgTrp: 0.656 ± 0.205
2.033ArgTyr: 2.033 ± 0.323
0.0ArgXaa: 0.0 ± 0.0
Ser
5.114SerAla: 5.114 ± 0.76
1.049SerCys: 1.049 ± 0.274
4.065SerAsp: 4.065 ± 0.524
3.737SerGlu: 3.737 ± 0.438
3.147SerPhe: 3.147 ± 0.358
6.294SerGly: 6.294 ± 0.593
0.918SerHis: 0.918 ± 0.27
4.131SerIle: 4.131 ± 0.506
3.934SerLys: 3.934 ± 0.482
5.77SerLeu: 5.77 ± 0.642
1.836SerMet: 1.836 ± 0.301
2.229SerAsn: 2.229 ± 0.517
2.164SerPro: 2.164 ± 0.385
2.819SerGln: 2.819 ± 0.388
3.016SerArg: 3.016 ± 0.346
3.737SerSer: 3.737 ± 0.462
3.541SerThr: 3.541 ± 0.421
5.18SerVal: 5.18 ± 0.46
0.918SerTrp: 0.918 ± 0.217
1.77SerTyr: 1.77 ± 0.409
0.0SerXaa: 0.0 ± 0.0
Thr
4.262ThrAla: 4.262 ± 0.485
1.115ThrCys: 1.115 ± 0.233
3.541ThrAsp: 3.541 ± 0.52
3.016ThrGlu: 3.016 ± 0.467
2.491ThrPhe: 2.491 ± 0.426
4.786ThrGly: 4.786 ± 0.463
1.115ThrHis: 1.115 ± 0.288
4.131ThrIle: 4.131 ± 0.416
3.082ThrLys: 3.082 ± 0.437
3.213ThrLeu: 3.213 ± 0.448
1.77ThrMet: 1.77 ± 0.301
2.557ThrAsn: 2.557 ± 0.481
2.95ThrPro: 2.95 ± 0.508
2.098ThrGln: 2.098 ± 0.32
2.229ThrArg: 2.229 ± 0.361
3.737ThrSer: 3.737 ± 0.405
2.623ThrThr: 2.623 ± 0.374
3.606ThrVal: 3.606 ± 0.493
0.787ThrTrp: 0.787 ± 0.197
1.901ThrTyr: 1.901 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
5.114ValAla: 5.114 ± 0.628
0.59ValCys: 0.59 ± 0.183
4.59ValAsp: 4.59 ± 0.634
3.868ValGlu: 3.868 ± 0.485
3.213ValPhe: 3.213 ± 0.464
3.803ValGly: 3.803 ± 0.608
1.377ValHis: 1.377 ± 0.316
3.999ValIle: 3.999 ± 0.531
6.622ValLys: 6.622 ± 0.73
3.475ValLeu: 3.475 ± 0.464
1.967ValMet: 1.967 ± 0.378
3.672ValAsn: 3.672 ± 0.566
2.491ValPro: 2.491 ± 0.472
2.95ValGln: 2.95 ± 0.402
4.065ValArg: 4.065 ± 0.56
5.245ValSer: 5.245 ± 0.539
4.524ValThr: 4.524 ± 0.531
4.59ValVal: 4.59 ± 0.62
1.246ValTrp: 1.246 ± 0.244
2.098ValTyr: 2.098 ± 0.425
0.0ValXaa: 0.0 ± 0.0
Trp
1.049TrpAla: 1.049 ± 0.29
0.393TrpCys: 0.393 ± 0.234
0.852TrpAsp: 0.852 ± 0.229
0.852TrpGlu: 0.852 ± 0.244
0.459TrpPhe: 0.459 ± 0.216
0.852TrpGly: 0.852 ± 0.193
0.328TrpHis: 0.328 ± 0.158
0.721TrpIle: 0.721 ± 0.225
0.918TrpLys: 0.918 ± 0.211
1.049TrpLeu: 1.049 ± 0.23
0.459TrpMet: 0.459 ± 0.151
0.918TrpAsn: 0.918 ± 0.188
0.328TrpPro: 0.328 ± 0.151
0.59TrpGln: 0.59 ± 0.167
0.918TrpArg: 0.918 ± 0.261
0.918TrpSer: 0.918 ± 0.219
1.311TrpThr: 1.311 ± 0.351
0.983TrpVal: 0.983 ± 0.202
0.262TrpTrp: 0.262 ± 0.145
0.459TrpTyr: 0.459 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.95TyrAla: 2.95 ± 0.396
0.262TyrCys: 0.262 ± 0.121
2.754TyrAsp: 2.754 ± 0.451
2.491TyrGlu: 2.491 ± 0.347
1.377TyrPhe: 1.377 ± 0.307
2.688TyrGly: 2.688 ± 0.394
0.525TyrHis: 0.525 ± 0.168
2.688TyrIle: 2.688 ± 0.497
2.098TyrLys: 2.098 ± 0.312
1.639TyrLeu: 1.639 ± 0.294
1.508TyrMet: 1.508 ± 0.322
1.967TyrAsn: 1.967 ± 0.295
1.18TyrPro: 1.18 ± 0.276
1.311TyrGln: 1.311 ± 0.271
1.967TyrArg: 1.967 ± 0.346
2.098TyrSer: 2.098 ± 0.313
2.164TyrThr: 2.164 ± 0.384
1.574TyrVal: 1.574 ± 0.335
0.262TyrTrp: 0.262 ± 0.144
1.639TyrTyr: 1.639 ± 0.297
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (15253 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski