Rhodobacteraceae bacterium 9Alg 56
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3843 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P1SXS7|A0A6P1SXS7_9RHOB SCP domain-containing protein OS=Rhodobacteraceae bacterium 9Alg 56 OX=2683284 GN=GO499_09925 PE=4 SV=1
MM1 pKa = 7.27 TEE3 pKa = 3.11 IRR5 pKa = 11.84 NFIFGNSLINHH16 pKa = 5.86 VSGSHH21 pKa = 4.44 QTTVPYY27 pKa = 10.08 WLDD30 pKa = 3.32 ALADD34 pKa = 4.16 TAGHH38 pKa = 6.3 SYY40 pKa = 11.01 SVDD43 pKa = 3.24 GQFGNLLSHH52 pKa = 7.28 LGTNPIPNWWFGDD65 pKa = 3.78 GVEE68 pKa = 4.68 GSWNSSFANSDD79 pKa = 3.45 YY80 pKa = 9.76 TNVMITTANYY90 pKa = 9.5 IQYY93 pKa = 9.93 QGPDD97 pKa = 2.91 EE98 pKa = 4.07 QYY100 pKa = 10.28 YY101 pKa = 10.51 GNSHH105 pKa = 5.69 TPISATLEE113 pKa = 4.1 LVDD116 pKa = 3.86 MVHH119 pKa = 6.82 ARR121 pKa = 11.84 EE122 pKa = 4.08 PNAVVDD128 pKa = 5.43 IYY130 pKa = 11.61 VGWPDD135 pKa = 3.38 MAGVFPNFPPSDD147 pKa = 3.85 AQFDD151 pKa = 4.3 TYY153 pKa = 11.61 NQLTMTTYY161 pKa = 9.88 MEE163 pKa = 4.61 WYY165 pKa = 9.7 DD166 pKa = 3.69 EE167 pKa = 4.39 YY168 pKa = 11.73 YY169 pKa = 11.02 AALQAARR176 pKa = 11.84 PGVTMNLMPVGPAMAHH192 pKa = 6.89 LFTTVLSDD200 pKa = 3.16 VSAADD205 pKa = 4.05 LYY207 pKa = 11.29 EE208 pKa = 5.15 DD209 pKa = 4.01 NAPHH213 pKa = 5.76 GTEE216 pKa = 3.66 TLYY219 pKa = 11.13 FLASLATYY227 pKa = 10.82 AFMYY231 pKa = 10.57 DD232 pKa = 4.04 EE233 pKa = 5.55 LPPADD238 pKa = 3.95 FQIPNNILPSVRR250 pKa = 11.84 AAYY253 pKa = 10.6 SDD255 pKa = 3.4 ILTEE259 pKa = 4.21 LADD262 pKa = 3.79 FTGIDD267 pKa = 3.53 PAEE270 pKa = 4.17 VEE272 pKa = 4.49 GGSDD276 pKa = 3.81 GDD278 pKa = 3.93 SSGGGVDD285 pKa = 4.17 VEE287 pKa = 4.57 PEE289 pKa = 4.23 VNVVDD294 pKa = 5.12 GDD296 pKa = 3.86 DD297 pKa = 3.56 RR298 pKa = 11.84 NNNIVGTDD306 pKa = 3.28 GMDD309 pKa = 4.3 AISGLGGNDD318 pKa = 3.56 TIQAGDD324 pKa = 3.7 GNDD327 pKa = 4.02 TIDD330 pKa = 4.01 AGAGNDD336 pKa = 4.78 SVWGDD341 pKa = 3.39 QGADD345 pKa = 3.62 NIHH348 pKa = 6.54 GGDD351 pKa = 3.58 GSDD354 pKa = 3.55 FLSGGRR360 pKa = 11.84 GNDD363 pKa = 3.18 VVEE366 pKa = 4.77 GNSGDD371 pKa = 3.42 DD372 pKa = 4.01 WINGNYY378 pKa = 10.28 GNDD381 pKa = 3.15 ILNGGGGNDD390 pKa = 4.35 TINAGAGRR398 pKa = 11.84 DD399 pKa = 3.63 TIYY402 pKa = 10.97 AGNGDD407 pKa = 3.57 DD408 pKa = 5.05 RR409 pKa = 11.84 VRR411 pKa = 11.84 GGNGRR416 pKa = 11.84 DD417 pKa = 3.38 TVYY420 pKa = 10.88 LGAGHH425 pKa = 7.51 DD426 pKa = 3.48 RR427 pKa = 11.84 FTDD430 pKa = 3.33 NGQTGEE436 pKa = 4.19 NAVDD440 pKa = 3.49 TVYY443 pKa = 11.21 GGDD446 pKa = 3.77 GDD448 pKa = 4.67 DD449 pKa = 5.12 AIHH452 pKa = 7.0 GGGGDD457 pKa = 3.4 DD458 pKa = 3.31 RR459 pKa = 11.84 FYY461 pKa = 11.74 GEE463 pKa = 4.84 GGNDD467 pKa = 3.67 RR468 pKa = 11.84 IFGGSGNDD476 pKa = 3.59 QIRR479 pKa = 11.84 GGAGDD484 pKa = 4.25 DD485 pKa = 3.71 SLHH488 pKa = 5.84 GRR490 pKa = 11.84 EE491 pKa = 4.23 GADD494 pKa = 3.62 TIFGGNGHH502 pKa = 6.8 DD503 pKa = 4.82 FINAGSGNDD512 pKa = 3.46 TVEE515 pKa = 4.4 GGNGRR520 pKa = 11.84 DD521 pKa = 3.22 RR522 pKa = 11.84 VRR524 pKa = 11.84 LGGGDD529 pKa = 4.09 DD530 pKa = 3.48 RR531 pKa = 11.84 FVDD534 pKa = 3.43 NDD536 pKa = 3.19 QAGFQGSDD544 pKa = 3.29 TVSGGAGNDD553 pKa = 3.77 TILGGGGNDD562 pKa = 3.13 RR563 pKa = 11.84 LFGGSGADD571 pKa = 3.66 TINGGLEE578 pKa = 3.78 NDD580 pKa = 4.67 HH581 pKa = 6.88 ISGGEE586 pKa = 4.03 GADD589 pKa = 3.55 TLNGGWGDD597 pKa = 3.83 DD598 pKa = 4.56 FINGAQGADD607 pKa = 3.38 IINAGGGDD615 pKa = 3.43 DD616 pKa = 4.38 HH617 pKa = 8.97 VFGGLGADD625 pKa = 3.71 VVDD628 pKa = 5.45 LGDD631 pKa = 4.52 GNDD634 pKa = 3.52 IFQDD638 pKa = 3.59 SAEE641 pKa = 4.18 QGASGADD648 pKa = 3.36 TVYY651 pKa = 11.05 GGAGNDD657 pKa = 3.72 IINGGGGQDD666 pKa = 3.67 SLHH669 pKa = 6.67 GGDD672 pKa = 4.65 GDD674 pKa = 4.17 DD675 pKa = 3.64 EE676 pKa = 4.58 VRR678 pKa = 11.84 GGVGNDD684 pKa = 4.56 DD685 pKa = 3.46 ITGGAGNDD693 pKa = 3.73 VLHH696 pKa = 6.92 GGAGDD701 pKa = 3.96 DD702 pKa = 4.77 SIDD705 pKa = 3.69 GGGGADD711 pKa = 3.82 TIFGGTGNDD720 pKa = 3.45 TVSGGNGRR728 pKa = 11.84 DD729 pKa = 3.71 TVDD732 pKa = 3.79 LGAGNDD738 pKa = 3.68 VYY740 pKa = 11.33 TDD742 pKa = 3.4 TAQGDD747 pKa = 3.67 FHH749 pKa = 9.04 GEE751 pKa = 3.85 DD752 pKa = 4.21 TISGGAGNDD761 pKa = 3.82 RR762 pKa = 11.84 INGGGGNDD770 pKa = 4.66 TINGDD775 pKa = 3.77 NGSDD779 pKa = 3.55 TLFGGAGDD787 pKa = 3.99 DD788 pKa = 4.45 TIHH791 pKa = 6.72 GGAGVDD797 pKa = 3.65 VLNGGTGADD806 pKa = 3.32 ILTGGEE812 pKa = 4.09 GADD815 pKa = 3.33 TFVYY819 pKa = 10.26 TSVGQSNFAGGIDD832 pKa = 4.06 TITDD836 pKa = 4.07 FTRR839 pKa = 11.84 GTDD842 pKa = 3.42 IIDD845 pKa = 4.14 LSGIDD850 pKa = 4.15 ANTSVAGNQAFGFIGGLDD868 pKa = 3.61 FTGTAGEE875 pKa = 4.1 LRR877 pKa = 11.84 FANGQLLGDD886 pKa = 3.71 VDD888 pKa = 4.48 GDD890 pKa = 4.14 GNSDD894 pKa = 3.48 FSINLTGVGNLSGANLEE911 pKa = 4.49 LGVDD915 pKa = 4.01 EE916 pKa = 5.39 EE917 pKa = 4.61 PTDD920 pKa = 4.08 GEE922 pKa = 4.57 GGGGGGDD929 pKa = 3.74 PVILPNDD936 pKa = 3.7 NGQPAPEE943 pKa = 3.73 QDD945 pKa = 3.36 TFGEE949 pKa = 4.71 SNPSLGMGLEE959 pKa = 5.39 GIADD963 pKa = 3.93 WSSQAPFIDD972 pKa = 3.34 VMKK975 pKa = 10.34 FARR978 pKa = 11.84 PWIGHH983 pKa = 6.44 VGDD986 pKa = 3.13 QWGGYY991 pKa = 8.05 GHH993 pKa = 7.58 DD994 pKa = 3.55 RR995 pKa = 11.84 LEE997 pKa = 4.42 EE998 pKa = 4.04 NGFLDD1003 pKa = 4.12 EE1004 pKa = 5.24 NGWPTDD1010 pKa = 3.07 IPAGVSSIQAVVMADD1025 pKa = 3.64 LPEE1028 pKa = 4.49 EE1029 pKa = 4.28 SASLAGRR1036 pKa = 11.84 YY1037 pKa = 9.17 RR1038 pKa = 11.84 LTWEE1042 pKa = 4.22 GEE1044 pKa = 3.84 GDD1046 pKa = 3.72 VEE1048 pKa = 4.92 VIGGRR1053 pKa = 11.84 ISNITRR1059 pKa = 11.84 SNGEE1063 pKa = 3.2 IWFDD1067 pKa = 3.81 FEE1069 pKa = 4.95 PGGSGTVISISSTDD1083 pKa = 3.44 PNNNGNYY1090 pKa = 9.22 IRR1092 pKa = 11.84 DD1093 pKa = 3.55 IEE1095 pKa = 4.59 LVHH1098 pKa = 6.79 EE1099 pKa = 4.85 DD1100 pKa = 3.54 NIEE1103 pKa = 3.82 AHH1105 pKa = 5.97 EE1106 pKa = 4.73 AGAIFNPDD1114 pKa = 3.77 WIDD1117 pKa = 4.08 LIEE1120 pKa = 4.7 DD1121 pKa = 3.55 LRR1123 pKa = 11.84 SVRR1126 pKa = 11.84 FMDD1129 pKa = 4.14 WMQTNNSPQVDD1140 pKa = 3.31 WSDD1143 pKa = 3.28 RR1144 pKa = 11.84 PTPDD1148 pKa = 2.62 NYY1150 pKa = 10.68 TYY1152 pKa = 11.01 FDD1154 pKa = 3.36 GGVPVEE1160 pKa = 5.07 IMVQLANQIGADD1172 pKa = 3.28 PWFNMPHH1179 pKa = 5.54 QANDD1183 pKa = 2.8 EE1184 pKa = 4.2 YY1185 pKa = 10.7 MRR1187 pKa = 11.84 EE1188 pKa = 3.71 FAEE1191 pKa = 4.3 YY1192 pKa = 10.83 VRR1194 pKa = 11.84 DD1195 pKa = 3.85 NLDD1198 pKa = 3.52 PEE1200 pKa = 3.87 LRR1202 pKa = 11.84 AYY1204 pKa = 10.49 VEE1206 pKa = 4.23 YY1207 pKa = 11.21 SNEE1210 pKa = 3.17 VWNYY1214 pKa = 10.37 GFEE1217 pKa = 4.14 QAHH1220 pKa = 5.9 WAAEE1224 pKa = 3.92 QATARR1229 pKa = 11.84 WGNAANDD1236 pKa = 4.26 GGWMQFYY1243 pKa = 10.09 GARR1246 pKa = 11.84 AAEE1249 pKa = 4.15 MAIIWDD1255 pKa = 3.96 DD1256 pKa = 3.68 VFGNQADD1263 pKa = 3.61 DD1264 pKa = 3.49 RR1265 pKa = 11.84 VINVIGTHH1273 pKa = 5.5 TGWPGLEE1280 pKa = 3.25 QYY1282 pKa = 9.29 MFNSEE1287 pKa = 3.68 LWVRR1291 pKa = 11.84 EE1292 pKa = 4.08 TGNDD1296 pKa = 3.14 APYY1299 pKa = 10.02 TYY1301 pKa = 10.61 FDD1303 pKa = 3.84 AYY1305 pKa = 10.8 AVTGYY1310 pKa = 10.63 FGIEE1314 pKa = 4.16 LGNSRR1319 pKa = 11.84 AGEE1322 pKa = 3.87 VLGWIEE1328 pKa = 4.4 DD1329 pKa = 3.7 SRR1331 pKa = 11.84 NAAIADD1337 pKa = 3.74 ANAQGLTGSARR1348 pKa = 11.84 TRR1350 pKa = 11.84 YY1351 pKa = 9.43 IEE1353 pKa = 3.79 EE1354 pKa = 3.94 HH1355 pKa = 6.56 QYY1357 pKa = 11.39 DD1358 pKa = 3.8 QASAIAAEE1366 pKa = 4.25 DD1367 pKa = 3.78 LRR1369 pKa = 11.84 TGSLAEE1375 pKa = 4.89 LIGEE1379 pKa = 4.73 TYY1381 pKa = 10.42 AYY1383 pKa = 10.42 QSAAATAAGLEE1394 pKa = 4.07 MVMYY1398 pKa = 10.48 EE1399 pKa = 4.16 GGTHH1403 pKa = 4.37 VVGVGGNVNNAEE1415 pKa = 3.74 LAAFFNHH1422 pKa = 6.3 FNYY1425 pKa = 9.04 TPEE1428 pKa = 3.82 MAEE1431 pKa = 4.02 LYY1433 pKa = 10.04 EE1434 pKa = 4.35 ILLEE1438 pKa = 4.3 GWQAAGGGLFNAFVDD1453 pKa = 4.17 VSDD1456 pKa = 4.3 PSQYY1460 pKa = 11.11 GSWGALRR1467 pKa = 11.84 YY1468 pKa = 10.27 LEE1470 pKa = 5.19 DD1471 pKa = 5.65 DD1472 pKa = 3.59 NPRR1475 pKa = 11.84 WDD1477 pKa = 3.88 VLNDD1481 pKa = 3.29 FNNNVAAWWEE1491 pKa = 4.09 DD1492 pKa = 3.23 RR1493 pKa = 11.84 SADD1496 pKa = 3.7 TFDD1499 pKa = 3.04 QGVFINGTDD1508 pKa = 3.56 GNDD1511 pKa = 3.99 AIGGTAEE1518 pKa = 4.17 EE1519 pKa = 5.51 DD1520 pKa = 3.16 ILLGGRR1526 pKa = 11.84 GNDD1529 pKa = 3.29 VLSGYY1534 pKa = 10.39 GGEE1537 pKa = 4.42 DD1538 pKa = 3.29 RR1539 pKa = 11.84 LHH1541 pKa = 7.72 GGMGNDD1547 pKa = 3.87 TAWLIGSYY1555 pKa = 10.29 EE1556 pKa = 4.55 DD1557 pKa = 3.75 YY1558 pKa = 10.94 TFTSDD1563 pKa = 3.05 NGTVIATHH1571 pKa = 5.85 SSGRR1575 pKa = 11.84 TFLTDD1580 pKa = 3.04 IEE1582 pKa = 4.31 NVVFEE1587 pKa = 4.81 EE1588 pKa = 4.4 NPDD1591 pKa = 3.09 VAYY1594 pKa = 10.26 AANSLFF1600 pKa = 3.77
Molecular weight: 167.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.63
IPC_protein 3.694
Toseland 3.452
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.503
Grimsley 3.35
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.075
Thurlkill 3.503
EMBOSS 3.63
Sillero 3.808
Patrickios 1.85
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|A0A6P1SZC8|A0A6P1SZC8_9RHOB Cysteine desulfurase OS=Rhodobacteraceae bacterium 9Alg 56 OX=2683284 GN=GO499_06215 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 8.78 ILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.3 SLSAA44 pKa = 3.83
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3843
0
3843
1205218
31
2168
313.6
34.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.302 ± 0.05
0.899 ± 0.013
5.819 ± 0.033
6.339 ± 0.035
3.873 ± 0.025
8.775 ± 0.044
2.006 ± 0.02
5.209 ± 0.031
2.951 ± 0.034
10.061 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.02
2.623 ± 0.021
5.069 ± 0.03
2.973 ± 0.02
6.779 ± 0.037
5.34 ± 0.025
5.498 ± 0.028
7.242 ± 0.032
1.365 ± 0.017
2.196 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here