Rhodobacteraceae bacterium 9Alg 56

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6P1SXS7|A0A6P1SXS7_9RHOB SCP domain-containing protein OS=Rhodobacteraceae bacterium 9Alg 56 OX=2683284 GN=GO499_09925 PE=4 SV=1
MM1 pKa = 7.27TEE3 pKa = 3.11IRR5 pKa = 11.84NFIFGNSLINHH16 pKa = 5.86VSGSHH21 pKa = 4.44QTTVPYY27 pKa = 10.08WLDD30 pKa = 3.32ALADD34 pKa = 4.16TAGHH38 pKa = 6.3SYY40 pKa = 11.01SVDD43 pKa = 3.24GQFGNLLSHH52 pKa = 7.28LGTNPIPNWWFGDD65 pKa = 3.78GVEE68 pKa = 4.68GSWNSSFANSDD79 pKa = 3.45YY80 pKa = 9.76TNVMITTANYY90 pKa = 9.5IQYY93 pKa = 9.93QGPDD97 pKa = 2.91EE98 pKa = 4.07QYY100 pKa = 10.28YY101 pKa = 10.51GNSHH105 pKa = 5.69TPISATLEE113 pKa = 4.1LVDD116 pKa = 3.86MVHH119 pKa = 6.82ARR121 pKa = 11.84EE122 pKa = 4.08PNAVVDD128 pKa = 5.43IYY130 pKa = 11.61VGWPDD135 pKa = 3.38MAGVFPNFPPSDD147 pKa = 3.85AQFDD151 pKa = 4.3TYY153 pKa = 11.61NQLTMTTYY161 pKa = 9.88MEE163 pKa = 4.61WYY165 pKa = 9.7DD166 pKa = 3.69EE167 pKa = 4.39YY168 pKa = 11.73YY169 pKa = 11.02AALQAARR176 pKa = 11.84PGVTMNLMPVGPAMAHH192 pKa = 6.89LFTTVLSDD200 pKa = 3.16VSAADD205 pKa = 4.05LYY207 pKa = 11.29EE208 pKa = 5.15DD209 pKa = 4.01NAPHH213 pKa = 5.76GTEE216 pKa = 3.66TLYY219 pKa = 11.13FLASLATYY227 pKa = 10.82AFMYY231 pKa = 10.57DD232 pKa = 4.04EE233 pKa = 5.55LPPADD238 pKa = 3.95FQIPNNILPSVRR250 pKa = 11.84AAYY253 pKa = 10.6SDD255 pKa = 3.4ILTEE259 pKa = 4.21LADD262 pKa = 3.79FTGIDD267 pKa = 3.53PAEE270 pKa = 4.17VEE272 pKa = 4.49GGSDD276 pKa = 3.81GDD278 pKa = 3.93SSGGGVDD285 pKa = 4.17VEE287 pKa = 4.57PEE289 pKa = 4.23VNVVDD294 pKa = 5.12GDD296 pKa = 3.86DD297 pKa = 3.56RR298 pKa = 11.84NNNIVGTDD306 pKa = 3.28GMDD309 pKa = 4.3AISGLGGNDD318 pKa = 3.56TIQAGDD324 pKa = 3.7GNDD327 pKa = 4.02TIDD330 pKa = 4.01AGAGNDD336 pKa = 4.78SVWGDD341 pKa = 3.39QGADD345 pKa = 3.62NIHH348 pKa = 6.54GGDD351 pKa = 3.58GSDD354 pKa = 3.55FLSGGRR360 pKa = 11.84GNDD363 pKa = 3.18VVEE366 pKa = 4.77GNSGDD371 pKa = 3.42DD372 pKa = 4.01WINGNYY378 pKa = 10.28GNDD381 pKa = 3.15ILNGGGGNDD390 pKa = 4.35TINAGAGRR398 pKa = 11.84DD399 pKa = 3.63TIYY402 pKa = 10.97AGNGDD407 pKa = 3.57DD408 pKa = 5.05RR409 pKa = 11.84VRR411 pKa = 11.84GGNGRR416 pKa = 11.84DD417 pKa = 3.38TVYY420 pKa = 10.88LGAGHH425 pKa = 7.51DD426 pKa = 3.48RR427 pKa = 11.84FTDD430 pKa = 3.33NGQTGEE436 pKa = 4.19NAVDD440 pKa = 3.49TVYY443 pKa = 11.21GGDD446 pKa = 3.77GDD448 pKa = 4.67DD449 pKa = 5.12AIHH452 pKa = 7.0GGGGDD457 pKa = 3.4DD458 pKa = 3.31RR459 pKa = 11.84FYY461 pKa = 11.74GEE463 pKa = 4.84GGNDD467 pKa = 3.67RR468 pKa = 11.84IFGGSGNDD476 pKa = 3.59QIRR479 pKa = 11.84GGAGDD484 pKa = 4.25DD485 pKa = 3.71SLHH488 pKa = 5.84GRR490 pKa = 11.84EE491 pKa = 4.23GADD494 pKa = 3.62TIFGGNGHH502 pKa = 6.8DD503 pKa = 4.82FINAGSGNDD512 pKa = 3.46TVEE515 pKa = 4.4GGNGRR520 pKa = 11.84DD521 pKa = 3.22RR522 pKa = 11.84VRR524 pKa = 11.84LGGGDD529 pKa = 4.09DD530 pKa = 3.48RR531 pKa = 11.84FVDD534 pKa = 3.43NDD536 pKa = 3.19QAGFQGSDD544 pKa = 3.29TVSGGAGNDD553 pKa = 3.77TILGGGGNDD562 pKa = 3.13RR563 pKa = 11.84LFGGSGADD571 pKa = 3.66TINGGLEE578 pKa = 3.78NDD580 pKa = 4.67HH581 pKa = 6.88ISGGEE586 pKa = 4.03GADD589 pKa = 3.55TLNGGWGDD597 pKa = 3.83DD598 pKa = 4.56FINGAQGADD607 pKa = 3.38IINAGGGDD615 pKa = 3.43DD616 pKa = 4.38HH617 pKa = 8.97VFGGLGADD625 pKa = 3.71VVDD628 pKa = 5.45LGDD631 pKa = 4.52GNDD634 pKa = 3.52IFQDD638 pKa = 3.59SAEE641 pKa = 4.18QGASGADD648 pKa = 3.36TVYY651 pKa = 11.05GGAGNDD657 pKa = 3.72IINGGGGQDD666 pKa = 3.67SLHH669 pKa = 6.67GGDD672 pKa = 4.65GDD674 pKa = 4.17DD675 pKa = 3.64EE676 pKa = 4.58VRR678 pKa = 11.84GGVGNDD684 pKa = 4.56DD685 pKa = 3.46ITGGAGNDD693 pKa = 3.73VLHH696 pKa = 6.92GGAGDD701 pKa = 3.96DD702 pKa = 4.77SIDD705 pKa = 3.69GGGGADD711 pKa = 3.82TIFGGTGNDD720 pKa = 3.45TVSGGNGRR728 pKa = 11.84DD729 pKa = 3.71TVDD732 pKa = 3.79LGAGNDD738 pKa = 3.68VYY740 pKa = 11.33TDD742 pKa = 3.4TAQGDD747 pKa = 3.67FHH749 pKa = 9.04GEE751 pKa = 3.85DD752 pKa = 4.21TISGGAGNDD761 pKa = 3.82RR762 pKa = 11.84INGGGGNDD770 pKa = 4.66TINGDD775 pKa = 3.77NGSDD779 pKa = 3.55TLFGGAGDD787 pKa = 3.99DD788 pKa = 4.45TIHH791 pKa = 6.72GGAGVDD797 pKa = 3.65VLNGGTGADD806 pKa = 3.32ILTGGEE812 pKa = 4.09GADD815 pKa = 3.33TFVYY819 pKa = 10.26TSVGQSNFAGGIDD832 pKa = 4.06TITDD836 pKa = 4.07FTRR839 pKa = 11.84GTDD842 pKa = 3.42IIDD845 pKa = 4.14LSGIDD850 pKa = 4.15ANTSVAGNQAFGFIGGLDD868 pKa = 3.61FTGTAGEE875 pKa = 4.1LRR877 pKa = 11.84FANGQLLGDD886 pKa = 3.71VDD888 pKa = 4.48GDD890 pKa = 4.14GNSDD894 pKa = 3.48FSINLTGVGNLSGANLEE911 pKa = 4.49LGVDD915 pKa = 4.01EE916 pKa = 5.39EE917 pKa = 4.61PTDD920 pKa = 4.08GEE922 pKa = 4.57GGGGGGDD929 pKa = 3.74PVILPNDD936 pKa = 3.7NGQPAPEE943 pKa = 3.73QDD945 pKa = 3.36TFGEE949 pKa = 4.71SNPSLGMGLEE959 pKa = 5.39GIADD963 pKa = 3.93WSSQAPFIDD972 pKa = 3.34VMKK975 pKa = 10.34FARR978 pKa = 11.84PWIGHH983 pKa = 6.44VGDD986 pKa = 3.13QWGGYY991 pKa = 8.05GHH993 pKa = 7.58DD994 pKa = 3.55RR995 pKa = 11.84LEE997 pKa = 4.42EE998 pKa = 4.04NGFLDD1003 pKa = 4.12EE1004 pKa = 5.24NGWPTDD1010 pKa = 3.07IPAGVSSIQAVVMADD1025 pKa = 3.64LPEE1028 pKa = 4.49EE1029 pKa = 4.28SASLAGRR1036 pKa = 11.84YY1037 pKa = 9.17RR1038 pKa = 11.84LTWEE1042 pKa = 4.22GEE1044 pKa = 3.84GDD1046 pKa = 3.72VEE1048 pKa = 4.92VIGGRR1053 pKa = 11.84ISNITRR1059 pKa = 11.84SNGEE1063 pKa = 3.2IWFDD1067 pKa = 3.81FEE1069 pKa = 4.95PGGSGTVISISSTDD1083 pKa = 3.44PNNNGNYY1090 pKa = 9.22IRR1092 pKa = 11.84DD1093 pKa = 3.55IEE1095 pKa = 4.59LVHH1098 pKa = 6.79EE1099 pKa = 4.85DD1100 pKa = 3.54NIEE1103 pKa = 3.82AHH1105 pKa = 5.97EE1106 pKa = 4.73AGAIFNPDD1114 pKa = 3.77WIDD1117 pKa = 4.08LIEE1120 pKa = 4.7DD1121 pKa = 3.55LRR1123 pKa = 11.84SVRR1126 pKa = 11.84FMDD1129 pKa = 4.14WMQTNNSPQVDD1140 pKa = 3.31WSDD1143 pKa = 3.28RR1144 pKa = 11.84PTPDD1148 pKa = 2.62NYY1150 pKa = 10.68TYY1152 pKa = 11.01FDD1154 pKa = 3.36GGVPVEE1160 pKa = 5.07IMVQLANQIGADD1172 pKa = 3.28PWFNMPHH1179 pKa = 5.54QANDD1183 pKa = 2.8EE1184 pKa = 4.2YY1185 pKa = 10.7MRR1187 pKa = 11.84EE1188 pKa = 3.71FAEE1191 pKa = 4.3YY1192 pKa = 10.83VRR1194 pKa = 11.84DD1195 pKa = 3.85NLDD1198 pKa = 3.52PEE1200 pKa = 3.87LRR1202 pKa = 11.84AYY1204 pKa = 10.49VEE1206 pKa = 4.23YY1207 pKa = 11.21SNEE1210 pKa = 3.17VWNYY1214 pKa = 10.37GFEE1217 pKa = 4.14QAHH1220 pKa = 5.9WAAEE1224 pKa = 3.92QATARR1229 pKa = 11.84WGNAANDD1236 pKa = 4.26GGWMQFYY1243 pKa = 10.09GARR1246 pKa = 11.84AAEE1249 pKa = 4.15MAIIWDD1255 pKa = 3.96DD1256 pKa = 3.68VFGNQADD1263 pKa = 3.61DD1264 pKa = 3.49RR1265 pKa = 11.84VINVIGTHH1273 pKa = 5.5TGWPGLEE1280 pKa = 3.25QYY1282 pKa = 9.29MFNSEE1287 pKa = 3.68LWVRR1291 pKa = 11.84EE1292 pKa = 4.08TGNDD1296 pKa = 3.14APYY1299 pKa = 10.02TYY1301 pKa = 10.61FDD1303 pKa = 3.84AYY1305 pKa = 10.8AVTGYY1310 pKa = 10.63FGIEE1314 pKa = 4.16LGNSRR1319 pKa = 11.84AGEE1322 pKa = 3.87VLGWIEE1328 pKa = 4.4DD1329 pKa = 3.7SRR1331 pKa = 11.84NAAIADD1337 pKa = 3.74ANAQGLTGSARR1348 pKa = 11.84TRR1350 pKa = 11.84YY1351 pKa = 9.43IEE1353 pKa = 3.79EE1354 pKa = 3.94HH1355 pKa = 6.56QYY1357 pKa = 11.39DD1358 pKa = 3.8QASAIAAEE1366 pKa = 4.25DD1367 pKa = 3.78LRR1369 pKa = 11.84TGSLAEE1375 pKa = 4.89LIGEE1379 pKa = 4.73TYY1381 pKa = 10.42AYY1383 pKa = 10.42QSAAATAAGLEE1394 pKa = 4.07MVMYY1398 pKa = 10.48EE1399 pKa = 4.16GGTHH1403 pKa = 4.37VVGVGGNVNNAEE1415 pKa = 3.74LAAFFNHH1422 pKa = 6.3FNYY1425 pKa = 9.04TPEE1428 pKa = 3.82MAEE1431 pKa = 4.02LYY1433 pKa = 10.04EE1434 pKa = 4.35ILLEE1438 pKa = 4.3GWQAAGGGLFNAFVDD1453 pKa = 4.17VSDD1456 pKa = 4.3PSQYY1460 pKa = 11.11GSWGALRR1467 pKa = 11.84YY1468 pKa = 10.27LEE1470 pKa = 5.19DD1471 pKa = 5.65DD1472 pKa = 3.59NPRR1475 pKa = 11.84WDD1477 pKa = 3.88VLNDD1481 pKa = 3.29FNNNVAAWWEE1491 pKa = 4.09DD1492 pKa = 3.23RR1493 pKa = 11.84SADD1496 pKa = 3.7TFDD1499 pKa = 3.04QGVFINGTDD1508 pKa = 3.56GNDD1511 pKa = 3.99AIGGTAEE1518 pKa = 4.17EE1519 pKa = 5.51DD1520 pKa = 3.16ILLGGRR1526 pKa = 11.84GNDD1529 pKa = 3.29VLSGYY1534 pKa = 10.39GGEE1537 pKa = 4.42DD1538 pKa = 3.29RR1539 pKa = 11.84LHH1541 pKa = 7.72GGMGNDD1547 pKa = 3.87TAWLIGSYY1555 pKa = 10.29EE1556 pKa = 4.55DD1557 pKa = 3.75YY1558 pKa = 10.94TFTSDD1563 pKa = 3.05NGTVIATHH1571 pKa = 5.85SSGRR1575 pKa = 11.84TFLTDD1580 pKa = 3.04IEE1582 pKa = 4.31NVVFEE1587 pKa = 4.81EE1588 pKa = 4.4NPDD1591 pKa = 3.09VAYY1594 pKa = 10.26AANSLFF1600 pKa = 3.77

Molecular weight:
167.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6P1SZC8|A0A6P1SZC8_9RHOB Cysteine desulfurase OS=Rhodobacteraceae bacterium 9Alg 56 OX=2683284 GN=GO499_06215 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.78ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.3SLSAA44 pKa = 3.83

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3843

0

3843

1205218

31

2168

313.6

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.302 ± 0.05

0.899 ± 0.013

5.819 ± 0.033

6.339 ± 0.035

3.873 ± 0.025

8.775 ± 0.044

2.006 ± 0.02

5.209 ± 0.031

2.951 ± 0.034

10.061 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.68 ± 0.02

2.623 ± 0.021

5.069 ± 0.03

2.973 ± 0.02

6.779 ± 0.037

5.34 ± 0.025

5.498 ± 0.028

7.242 ± 0.032

1.365 ± 0.017

2.196 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski