Desulfonatronospira thiodismutans ASO3-1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3577 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D6SNR2|D6SNR2_9DELT Uncharacterized protein OS=Desulfonatronospira thiodismutans ASO3-1 OX=555779 GN=Dthio_PD1741 PE=4 SV=1
MM1 pKa = 7.95 LSGLLIAEE9 pKa = 4.96 IYY11 pKa = 11.05 GVMQNQFVLCIEE23 pKa = 4.74 NKK25 pKa = 10.58 DD26 pKa = 3.68 CDD28 pKa = 4.12 DD29 pKa = 3.79 LEE31 pKa = 4.23 KK32 pKa = 10.83 RR33 pKa = 11.84 KK34 pKa = 10.14 VYY36 pKa = 10.29 QVIPDD41 pKa = 3.91 EE42 pKa = 4.39 SASLDD47 pKa = 3.45 GYY49 pKa = 11.12 LRR51 pKa = 11.84 VIDD54 pKa = 4.66 EE55 pKa = 4.67 SGDD58 pKa = 3.52 DD59 pKa = 3.66 YY60 pKa = 11.56 LYY62 pKa = 10.45 PEE64 pKa = 4.26 SFFVFVQLPQQGAEE78 pKa = 4.11 VFQVSGQQ85 pKa = 3.34
Molecular weight: 9.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.897
IPC_protein 3.808
Toseland 3.617
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.935
Patrickios 0.769
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|D6SNI5|D6SNI5_9DELT Uncharacterized protein OS=Desulfonatronospira thiodismutans ASO3-1 OX=555779 GN=Dthio_PD1662 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 9.91 QPSRR9 pKa = 11.84 IKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 7.57 RR14 pKa = 11.84 THH16 pKa = 5.81 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.29 NGRR28 pKa = 11.84 AIINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.64 GRR39 pKa = 11.84 KK40 pKa = 8.66 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.427
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.149
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.093
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3577
0
3577
1095486
30
3984
306.3
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.566 ± 0.043
1.24 ± 0.021
5.931 ± 0.051
7.337 ± 0.047
4.192 ± 0.028
7.245 ± 0.036
2.28 ± 0.018
5.805 ± 0.035
5.17 ± 0.047
10.415 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.872 ± 0.022
3.443 ± 0.027
4.458 ± 0.028
4.31 ± 0.026
6.149 ± 0.049
6.177 ± 0.029
4.523 ± 0.033
6.649 ± 0.038
1.213 ± 0.016
3.024 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here