Desulfonatronospira thiodismutans ASO3-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfohalobiaceae; Desulfonatronospira; Desulfonatronospira thiodismutans

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3577 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D6SNR2|D6SNR2_9DELT Uncharacterized protein OS=Desulfonatronospira thiodismutans ASO3-1 OX=555779 GN=Dthio_PD1741 PE=4 SV=1
MM1 pKa = 7.95LSGLLIAEE9 pKa = 4.96IYY11 pKa = 11.05GVMQNQFVLCIEE23 pKa = 4.74NKK25 pKa = 10.58DD26 pKa = 3.68CDD28 pKa = 4.12DD29 pKa = 3.79LEE31 pKa = 4.23KK32 pKa = 10.83RR33 pKa = 11.84KK34 pKa = 10.14VYY36 pKa = 10.29QVIPDD41 pKa = 3.91EE42 pKa = 4.39SASLDD47 pKa = 3.45GYY49 pKa = 11.12LRR51 pKa = 11.84VIDD54 pKa = 4.66EE55 pKa = 4.67SGDD58 pKa = 3.52DD59 pKa = 3.66YY60 pKa = 11.56LYY62 pKa = 10.45PEE64 pKa = 4.26SFFVFVQLPQQGAEE78 pKa = 4.11VFQVSGQQ85 pKa = 3.34

Molecular weight:
9.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D6SNI5|D6SNI5_9DELT Uncharacterized protein OS=Desulfonatronospira thiodismutans ASO3-1 OX=555779 GN=Dthio_PD1662 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.91QPSRR9 pKa = 11.84IKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.81GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.29NGRR28 pKa = 11.84AIINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.64GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.41 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3577

0

3577

1095486

30

3984

306.3

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.566 ± 0.043

1.24 ± 0.021

5.931 ± 0.051

7.337 ± 0.047

4.192 ± 0.028

7.245 ± 0.036

2.28 ± 0.018

5.805 ± 0.035

5.17 ± 0.047

10.415 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.872 ± 0.022

3.443 ± 0.027

4.458 ± 0.028

4.31 ± 0.026

6.149 ± 0.049

6.177 ± 0.029

4.523 ± 0.033

6.649 ± 0.038

1.213 ± 0.016

3.024 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski