Planococcus sp. PAMC 21323
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3084 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4RDN9|A0A0B4RDN9_9BACL Uncharacterized protein OS=Planococcus sp. PAMC 21323 OX=1526927 GN=Plano_2314 PE=4 SV=1
MM1 pKa = 7.2 TRR3 pKa = 11.84 RR4 pKa = 11.84 TSKK7 pKa = 10.42 EE8 pKa = 3.53 IKK10 pKa = 8.95 KK11 pKa = 8.48 TNQIIAALSIALVLVMALGIYY32 pKa = 10.82 LMMKK36 pKa = 9.62 PDD38 pKa = 4.39 LPAEE42 pKa = 4.14 TRR44 pKa = 11.84 PPQSTQQQTEE54 pKa = 4.1 SGTPEE59 pKa = 4.38 AEE61 pKa = 4.05 TEE63 pKa = 4.15 LASMIANAKK72 pKa = 5.94 THH74 pKa = 6.26 VYY76 pKa = 9.01 TLYY79 pKa = 10.62 TDD81 pKa = 4.87 LEE83 pKa = 4.35 QGSGFLINNQGDD95 pKa = 3.95 ILTNAHH101 pKa = 5.65 VVLDD105 pKa = 3.96 ASYY108 pKa = 8.65 ITVKK112 pKa = 10.86 NSDD115 pKa = 3.36 GQEE118 pKa = 4.04 FNGNLIGISEE128 pKa = 4.39 TQDD131 pKa = 3.37 LAIVRR136 pKa = 11.84 VSEE139 pKa = 4.18 LAGKK143 pKa = 9.74 EE144 pKa = 3.74 PLEE147 pKa = 4.66 IEE149 pKa = 4.44 MEE151 pKa = 4.21 PVDD154 pKa = 3.24 IGTPVVAIGSPNDD167 pKa = 3.43 QSNTATIGEE176 pKa = 4.28 ITDD179 pKa = 3.79 TEE181 pKa = 5.05 LDD183 pKa = 3.56 FSDD186 pKa = 3.23 QFEE189 pKa = 4.51 YY190 pKa = 10.94 TNLYY194 pKa = 9.79 EE195 pKa = 4.16 MSAEE199 pKa = 3.9 IAQGSSGGPLIAADD213 pKa = 3.68 TGKK216 pKa = 10.77 LLGINSIIIEE226 pKa = 4.27 EE227 pKa = 4.24 NPDD230 pKa = 3.2 LGYY233 pKa = 10.65 AIPIYY238 pKa = 8.4 TVWDD242 pKa = 3.63 QLTEE246 pKa = 4.58 WINDD250 pKa = 3.77 PLITEE255 pKa = 4.13 EE256 pKa = 4.48 EE257 pKa = 4.51 EE258 pKa = 4.96 IVLQDD263 pKa = 3.55 VKK265 pKa = 11.03 DD266 pKa = 3.81 AYY268 pKa = 10.92 FDD270 pKa = 3.82 EE271 pKa = 5.87 DD272 pKa = 3.89 FLAGFISAYY281 pKa = 10.65 YY282 pKa = 9.92 EE283 pKa = 3.93 LLPYY287 pKa = 10.39 SLNDD291 pKa = 3.65 RR292 pKa = 11.84 EE293 pKa = 4.46 STYY296 pKa = 10.97 YY297 pKa = 9.52 MSYY300 pKa = 10.53 IFPGSNAEE308 pKa = 4.0 QQATGLIEE316 pKa = 4.7 EE317 pKa = 4.64 YY318 pKa = 10.7 SDD320 pKa = 3.73 PNLIYY325 pKa = 10.75 DD326 pKa = 3.8 VVKK329 pKa = 8.98 PTITSIEE336 pKa = 4.01 IEE338 pKa = 4.17 GDD340 pKa = 3.55 SALVEE345 pKa = 4.26 AEE347 pKa = 4.68 AEE349 pKa = 4.1 FTYY352 pKa = 10.6 HH353 pKa = 7.54 DD354 pKa = 4.05 KK355 pKa = 11.48 ASDD358 pKa = 3.34 KK359 pKa = 11.23 SSTISHH365 pKa = 6.62 KK366 pKa = 10.87 GMYY369 pKa = 8.65 TVVIDD374 pKa = 4.44 EE375 pKa = 4.51 YY376 pKa = 11.55 GDD378 pKa = 3.67 YY379 pKa = 10.99 QIDD382 pKa = 4.56 GIVNQQ387 pKa = 4.23
Molecular weight: 42.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.668
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.77
Nozaki 3.923
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.973
Patrickios 1.049
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.849
Protein with the highest isoelectric point:
>tr|A0A0B4RGE3|A0A0B4RGE3_9BACL Anthranilate synthase amidotransferase component OS=Planococcus sp. PAMC 21323 OX=1526927 GN=Plano_2877 PE=4 SV=1
MM1 pKa = 7.61 TLRR4 pKa = 11.84 TYY6 pKa = 10.57 QPNTRR11 pKa = 11.84 KK12 pKa = 9.66 HH13 pKa = 5.87 SKK15 pKa = 8.83 VHH17 pKa = 5.7 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 9.52 NGRR29 pKa = 11.84 RR30 pKa = 11.84 IIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.83 GRR40 pKa = 11.84 KK41 pKa = 8.75 VLSAA45 pKa = 4.05
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3084
0
3084
915920
37
1846
297.0
33.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.696 ± 0.048
0.563 ± 0.013
5.243 ± 0.038
7.705 ± 0.055
4.598 ± 0.033
6.93 ± 0.047
1.997 ± 0.02
7.534 ± 0.04
6.336 ± 0.042
9.823 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.92 ± 0.024
4.051 ± 0.033
3.6 ± 0.019
3.822 ± 0.03
4.003 ± 0.028
6.067 ± 0.03
5.643 ± 0.028
7.167 ± 0.034
1.0 ± 0.016
3.3 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here