Prosthecochloris sp. CIB 2401

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Prosthecochloris; unclassified Prosthecochloris

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1Q2C6|A0A1B1Q2C6_9CHLB Putative FAD-binding dehydrogenase OS=Prosthecochloris sp. CIB 2401 OX=1868325 GN=Ptc2401_01238 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.05KK3 pKa = 9.48IHH5 pKa = 6.96DD6 pKa = 3.87TTTANNWLQRR16 pKa = 11.84CAKK19 pKa = 9.21TALLVFGVLMPIAGPLTAHH38 pKa = 6.34AVEE41 pKa = 4.97SSVQSTARR49 pKa = 11.84PFGLDD54 pKa = 2.58IVAPVYY60 pKa = 10.2EE61 pKa = 4.82AGSDD65 pKa = 3.48EE66 pKa = 4.53AAVTFQTEE74 pKa = 4.14YY75 pKa = 11.31LPTLNDD81 pKa = 4.33WIDD84 pKa = 3.38QTLGEE89 pKa = 4.74TVSLDD94 pKa = 3.74NISSYY99 pKa = 11.64SLDD102 pKa = 3.64PSQLTLTTASDD113 pKa = 3.32VRR115 pKa = 11.84VYY117 pKa = 10.21FIGEE121 pKa = 3.75GAGYY125 pKa = 10.68KK126 pKa = 8.77NTLGFYY132 pKa = 10.29TDD134 pKa = 3.76GSDD137 pKa = 5.92DD138 pKa = 3.49ITTGDD143 pKa = 3.55AQLIFPNLTSNVSYY157 pKa = 10.87MNATDD162 pKa = 5.08LSNASANINFPLVPGDD178 pKa = 4.15FVDD181 pKa = 5.3LGTMDD186 pKa = 5.09AGTEE190 pKa = 3.84LDD192 pKa = 4.24FFLIADD198 pKa = 4.62GASGGTNVYY207 pKa = 9.09STDD210 pKa = 3.16ASLNPDD216 pKa = 4.17GIDD219 pKa = 3.15HH220 pKa = 5.53VVAYY224 pKa = 9.96AVEE227 pKa = 4.34DD228 pKa = 3.59SPYY231 pKa = 10.84LLVGFEE237 pKa = 4.66DD238 pKa = 4.37LFGGGDD244 pKa = 3.27QDD246 pKa = 4.29YY247 pKa = 11.08NDD249 pKa = 3.72VLFAIDD255 pKa = 3.5IGSINVDD262 pKa = 3.31ALTTASEE269 pKa = 4.23PSTVLLLGSFLLLAIYY285 pKa = 9.76LKK287 pKa = 10.68NRR289 pKa = 11.84QQHH292 pKa = 5.74LAAEE296 pKa = 4.35RR297 pKa = 11.84QNAA300 pKa = 3.28

Molecular weight: 32.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1Q200|A0A1B1Q200_9CHLB Stalked cell differentiation-controlling protein OS=Prosthecochloris sp. CIB 2401 OX=1868325 GN=pleD PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 4.21HH3 pKa = 6.37TNRR6 pKa = 11.84EE7 pKa = 4.08KK8 pKa = 10.73AIHH11 pKa = 6.44AFMSGRR17 pKa = 11.84INGRR21 pKa = 11.84VYY23 pKa = 10.99SRR25 pKa = 11.84AARR28 pKa = 11.84IFRR31 pKa = 11.84AGSFHH36 pKa = 7.43FMLAMLALCSVSLFTLGEE54 pKa = 4.03HH55 pKa = 5.96KK56 pKa = 10.48YY57 pKa = 10.69EE58 pKa = 4.9RR59 pKa = 11.84IRR61 pKa = 11.84APRR64 pKa = 11.84FKK66 pKa = 10.47YY67 pKa = 10.33GRR69 pKa = 11.84STHH72 pKa = 6.93DD73 pKa = 4.62PIHH76 pKa = 6.51ACVEE80 pKa = 4.28VMSQLL85 pKa = 3.49

Molecular weight: 9.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2188

0

2188

727722

30

17345

332.6

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.788 ± 0.081

1.028 ± 0.035

5.569 ± 0.092

7.049 ± 0.057

4.156 ± 0.042

7.785 ± 0.122

2.18 ± 0.049

6.206 ± 0.043

4.619 ± 0.098

10.248 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.038

3.329 ± 0.034

4.202 ± 0.074

3.416 ± 0.034

5.94 ± 0.104

6.364 ± 0.093

5.293 ± 0.1

7.098 ± 0.064

1.06 ± 0.025

2.978 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski