Mongoose feces-associated gemycircularvirus c
Average proteome isoelectric point is 7.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3JHI1|A0A0E3JHI1_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus c OX=1634488 PE=3 SV=1
MM1 pKa = 7.83 SSFRR5 pKa = 11.84 FQARR9 pKa = 11.84 YY10 pKa = 10.11 GLFTYY15 pKa = 7.62 SQCGQLRR22 pKa = 11.84 PSAVVEE28 pKa = 4.27 LFSSLSADD36 pKa = 3.53 CIVGRR41 pKa = 11.84 EE42 pKa = 3.97 AHH44 pKa = 6.62 GDD46 pKa = 3.59 GGTHH50 pKa = 6.01 LHH52 pKa = 6.52 AFVDD56 pKa = 4.76 FRR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 9.82 YY61 pKa = 8.38 RR62 pKa = 11.84 TRR64 pKa = 11.84 DD65 pKa = 3.08 VRR67 pKa = 11.84 KK68 pKa = 8.76 FDD70 pKa = 3.82 VEE72 pKa = 4.29 GFHH75 pKa = 7.04 PNIISTIRR83 pKa = 11.84 SPSGSWDD90 pKa = 3.46 YY91 pKa = 10.31 ATKK94 pKa = 10.88 DD95 pKa = 3.14 GDD97 pKa = 3.76 VCGGSLRR104 pKa = 11.84 RR105 pKa = 11.84 PEE107 pKa = 4.3 PSQGSNAEE115 pKa = 4.02 RR116 pKa = 11.84 QNAGWDD122 pKa = 3.55 QLRR125 pKa = 11.84 DD126 pKa = 3.16 ASTRR130 pKa = 11.84 EE131 pKa = 3.56 EE132 pKa = 4.16 FLNLRR137 pKa = 11.84 QRR139 pKa = 11.84 IFSAISSNHH148 pKa = 5.62 SLHH151 pKa = 6.82 SLHH154 pKa = 5.94 TQIGSLEE161 pKa = 4.38 WIDD164 pKa = 3.88 HH165 pKa = 6.68 LIVTLANTRR174 pKa = 11.84 SILHH178 pKa = 5.87 NMVTFGNGLGDD189 pKa = 3.53 IYY191 pKa = 11.45 VWGPSRR197 pKa = 11.84 TGKK200 pKa = 8.59 TMWARR205 pKa = 11.84 SLGKK209 pKa = 9.22 HH210 pKa = 5.95 AYY212 pKa = 9.85 FGGLFSLEE220 pKa = 4.68 EE221 pKa = 4.14 YY222 pKa = 10.35 EE223 pKa = 4.64 QDD225 pKa = 3.58 PDD227 pKa = 3.07 CSYY230 pKa = 11.93 AVFDD234 pKa = 5.63 DD235 pKa = 3.64 IQGGFKK241 pKa = 10.46 FFPGYY246 pKa = 10.3 KK247 pKa = 9.24 SWLGQQTEE255 pKa = 5.26 FYY257 pKa = 9.95 CTDD260 pKa = 3.35 RR261 pKa = 11.84 YY262 pKa = 10.36 RR263 pKa = 11.84 KK264 pKa = 9.29 KK265 pKa = 10.77 KK266 pKa = 9.59 HH267 pKa = 5.74 IAWGKK272 pKa = 7.54 PCIWLSNNNPMGEE285 pKa = 4.28 DD286 pKa = 3.9 ADD288 pKa = 4.7 HH289 pKa = 7.46 DD290 pKa = 4.16 WLEE293 pKa = 4.16 KK294 pKa = 10.65 NCIIVYY300 pKa = 10.33 VGDD303 pKa = 3.49 RR304 pKa = 11.84 LYY306 pKa = 11.41
Molecular weight: 34.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.594
IPC2_protein 6.59
IPC_protein 6.737
Toseland 6.781
ProMoST 7.293
Dawson 7.234
Bjellqvist 7.19
Wikipedia 7.205
Rodwell 7.234
Grimsley 6.898
Solomon 7.278
Lehninger 7.278
Nozaki 7.512
DTASelect 7.541
Thurlkill 7.571
EMBOSS 7.585
Sillero 7.673
Patrickios 4.101
IPC_peptide 7.278
IPC2_peptide 7.249
IPC2.peptide.svr19 7.035
Protein with the highest isoelectric point:
>tr|A0A0E3JHI1|A0A0E3JHI1_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus c OX=1634488 PE=3 SV=1
MM1 pKa = 7.72 AYY3 pKa = 10.24 ARR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 FRR9 pKa = 11.84 KK10 pKa = 7.9 RR11 pKa = 11.84 TYY13 pKa = 10.19 ARR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 6.01 PTSRR22 pKa = 11.84 RR23 pKa = 11.84 KK24 pKa = 9.45 PSRR27 pKa = 11.84 RR28 pKa = 11.84 SYY30 pKa = 9.57 GRR32 pKa = 11.84 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SSGRR39 pKa = 11.84 SVRR42 pKa = 11.84 SIRR45 pKa = 11.84 NIAARR50 pKa = 11.84 KK51 pKa = 9.51 CRR53 pKa = 11.84 DD54 pKa = 2.87 NMMGVPVSDD63 pKa = 3.91 TNIAGPPAPVQLTAGTDD80 pKa = 3.45 YY81 pKa = 11.62 GFLFCPSARR90 pKa = 11.84 RR91 pKa = 11.84 VGSLDD96 pKa = 2.88 GSLPRR101 pKa = 11.84 SIHH104 pKa = 5.9 ASASEE109 pKa = 3.93 RR110 pKa = 11.84 WKK112 pKa = 8.76 TRR114 pKa = 11.84 CYY116 pKa = 10.5 IKK118 pKa = 10.68 GLQEE122 pKa = 4.18 TIEE125 pKa = 4.05 VQPTDD130 pKa = 2.94 GMGWLWRR137 pKa = 11.84 RR138 pKa = 11.84 IVFSCIGLLDD148 pKa = 4.05 EE149 pKa = 4.99 FPGVSVSVADD159 pKa = 3.68 NVNGYY164 pKa = 9.09 GRR166 pKa = 11.84 ALWNVRR172 pKa = 11.84 SGTAEE177 pKa = 3.85 AQPLFTEE184 pKa = 4.38 TANYY188 pKa = 9.96 LFEE191 pKa = 4.28 GTRR194 pKa = 11.84 FRR196 pKa = 11.84 DD197 pKa = 3.21 WVNPFTAKK205 pKa = 10.34 LDD207 pKa = 3.75 RR208 pKa = 11.84 SIVTVHH214 pKa = 7.01 ADD216 pKa = 3.38 TVRR219 pKa = 11.84 TVQSNNPNGTYY230 pKa = 10.2 KK231 pKa = 10.39 VYY233 pKa = 10.75 KK234 pKa = 9.7 RR235 pKa = 11.84 YY236 pKa = 10.34 YY237 pKa = 8.05 PINRR241 pKa = 11.84 GIVYY245 pKa = 10.49 ADD247 pKa = 4.05 DD248 pKa = 3.63 EE249 pKa = 4.81 SGAGKK254 pKa = 10.6 KK255 pKa = 9.98 NDD257 pKa = 4.2 AIYY260 pKa = 10.51 AAGTTKK266 pKa = 10.7 GNSGDD271 pKa = 4.48 LFVLDD276 pKa = 4.06 MFSAVGDD283 pKa = 4.07 NEE285 pKa = 4.4 GSTLNFGVHH294 pKa = 6.13 ARR296 pKa = 11.84 YY297 pKa = 8.98 YY298 pKa = 7.44 WHH300 pKa = 7.27 EE301 pKa = 4.21 GSGQQ305 pKa = 3.18
Molecular weight: 34.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.305
IPC2_protein 9.706
IPC_protein 10.482
Toseland 10.189
ProMoST 10.043
Dawson 10.438
Bjellqvist 10.204
Wikipedia 10.687
Rodwell 10.555
Grimsley 10.54
Solomon 10.482
Lehninger 10.438
Nozaki 10.204
DTASelect 10.204
Thurlkill 10.277
EMBOSS 10.613
Sillero 10.365
Patrickios 9.823
IPC_peptide 10.482
IPC2_peptide 9.268
IPC2.peptide.svr19 8.522
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
611
305
306
305.5
34.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.71 ± 0.983
1.8 ± 0.323
6.056 ± 0.535
3.928 ± 0.429
4.91 ± 0.645
9.493 ± 0.01
2.619 ± 0.864
4.583 ± 0.429
3.764 ± 0.104
6.219 ± 0.86
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.473 ± 0.11
4.583 ± 0.438
3.928 ± 0.438
2.946 ± 0.647
9.82 ± 1.528
8.674 ± 0.316
5.565 ± 0.656
5.728 ± 0.982
2.455 ± 0.322
4.746 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here