Lizard adenovirus 2
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076FTC5|A0A076FTC5_9ADEN Protein ORF2 OS=Lizard adenovirus 2 OX=874272 PE=4 SV=1
MM1 pKa = 7.81 DD2 pKa = 4.38 TFGLDD7 pKa = 3.23 IVNMSTDD14 pKa = 3.89 EE15 pKa = 3.7 IAAILKK21 pKa = 10.32 KK22 pKa = 9.63 IDD24 pKa = 3.24 RR25 pKa = 11.84 FGYY28 pKa = 9.38 EE29 pKa = 4.78 LISINYY35 pKa = 8.97 DD36 pKa = 3.18 AFPFGPFRR44 pKa = 11.84 MSFSLGSDD52 pKa = 2.61 TVMYY56 pKa = 10.36 YY57 pKa = 10.69 VSQKK61 pKa = 11.1 DD62 pKa = 3.9 LALEE66 pKa = 4.12 WDD68 pKa = 4.85 PIQYY72 pKa = 10.7 LNWIRR77 pKa = 11.84 EE78 pKa = 4.12 ASDD81 pKa = 5.32 LNVNWVSCEE90 pKa = 3.52 RR91 pKa = 11.84 VPWWKK96 pKa = 10.08 IFPMLEE102 pKa = 3.89 EE103 pKa = 4.81 RR104 pKa = 11.84 KK105 pKa = 10.33 DD106 pKa = 3.91 SLWTCEE112 pKa = 3.72 NSVFFIPPSRR122 pKa = 11.84 SHH124 pKa = 7.03 PSWEE128 pKa = 3.81 YY129 pKa = 11.07 FNYY132 pKa = 9.81 FWSQCHH138 pKa = 6.09 SDD140 pKa = 3.61 APLDD144 pKa = 3.96 IGWSS148 pKa = 3.51
Molecular weight: 17.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.282
IPC2_protein 4.406
IPC_protein 4.329
Toseland 4.139
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.202
Rodwell 4.164
Grimsley 4.05
Solomon 4.291
Lehninger 4.24
Nozaki 4.406
DTASelect 4.609
Thurlkill 4.177
EMBOSS 4.215
Sillero 4.444
Patrickios 3.198
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.368
Protein with the highest isoelectric point:
>tr|A0A076FUM5|A0A076FUM5_9ADEN 100K OS=Lizard adenovirus 2 OX=874272 PE=4 SV=1
MM1 pKa = 7.51 ARR3 pKa = 11.84 LRR5 pKa = 11.84 LVTCRR10 pKa = 11.84 TARR13 pKa = 11.84 THH15 pKa = 4.23 TRR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 PRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 VTRR26 pKa = 11.84 SRR28 pKa = 11.84 TVTRR32 pKa = 11.84 TTRR35 pKa = 11.84 VTRR38 pKa = 11.84 RR39 pKa = 11.84 GHH41 pKa = 4.85 NVVYY45 pKa = 8.98 KK46 pKa = 8.94 TMRR49 pKa = 11.84 KK50 pKa = 5.48 TARR53 pKa = 11.84 IGSRR57 pKa = 11.84 RR58 pKa = 11.84 MRR60 pKa = 11.84 GGFLPLLAPILAAAIGAIPGIVVAARR86 pKa = 11.84 QKK88 pKa = 10.91
Molecular weight: 10.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.478
IPC2_protein 11.155
IPC_protein 12.647
Toseland 12.808
ProMoST 13.305
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.276
Rodwell 12.369
Grimsley 12.852
Solomon 13.305
Lehninger 13.203
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.305
Sillero 12.808
Patrickios 12.091
IPC_peptide 13.305
IPC2_peptide 12.296
IPC2.peptide.svr19 9.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
34
0
34
10779
57
1075
317.0
35.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.374 ± 0.303
2.301 ± 0.367
4.499 ± 0.234
5.956 ± 0.478
4.806 ± 0.27
6.244 ± 0.46
2.013 ± 0.203
4.49 ± 0.206
5.121 ± 0.547
9.676 ± 0.42
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.18 ± 0.111
5.167 ± 0.424
6.123 ± 0.369
4.166 ± 0.287
5.873 ± 0.559
7.672 ± 0.366
6.049 ± 0.389
6.086 ± 0.327
1.577 ± 0.146
3.627 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here