Leopards Hill virus
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B6VMT2|A0A0B6VMT2_9VIRU Glycoprotein G2 OS=Leopards Hill virus OX=1381104 GN=GP PE=4 SV=1
MM1 pKa = 7.75 SEE3 pKa = 5.54 LNFKK7 pKa = 10.19 DD8 pKa = 5.12 LNGLNNWFEE17 pKa = 4.22 EE18 pKa = 4.45 FKK20 pKa = 10.86 KK21 pKa = 10.61 DD22 pKa = 3.66 VPIYY26 pKa = 10.16 TSGANSACLSSGLVDD41 pKa = 4.33 LSQFKK46 pKa = 11.23 LEE48 pKa = 4.26 IDD50 pKa = 3.68 AQSSEE55 pKa = 4.28 SAKK58 pKa = 9.75 NAVYY62 pKa = 10.4 GRR64 pKa = 11.84 AVIAATRR71 pKa = 11.84 NLAPIYY77 pKa = 8.7 EE78 pKa = 4.69 CAWTSCEE85 pKa = 4.32 GIFEE89 pKa = 5.2 RR90 pKa = 11.84 SMSWFNDD97 pKa = 3.16 NADD100 pKa = 3.59 KK101 pKa = 11.3 PEE103 pKa = 3.99 FKK105 pKa = 10.26 VWHH108 pKa = 6.95 DD109 pKa = 3.85 KK110 pKa = 11.64 YY111 pKa = 11.71 NDD113 pKa = 3.62 LKK115 pKa = 11.51 SNLPEE120 pKa = 3.93 VEE122 pKa = 4.63 QILAYY127 pKa = 9.3 QNCSSLWRR135 pKa = 11.84 KK136 pKa = 10.24 DD137 pKa = 2.69 IGYY140 pKa = 9.09 EE141 pKa = 3.84 INQNTSTLKK150 pKa = 10.08 GDD152 pKa = 3.19 IVEE155 pKa = 4.39 EE156 pKa = 4.21 YY157 pKa = 10.57 GVNRR161 pKa = 11.84 NIVGTVKK168 pKa = 10.74 NMLVDD173 pKa = 3.66 MVNKK177 pKa = 10.01 RR178 pKa = 11.84 KK179 pKa = 9.86 KK180 pKa = 9.56 RR181 pKa = 11.84 LGGVVITGAGTSEE194 pKa = 4.55 DD195 pKa = 4.04 HH196 pKa = 6.02 ITWMEE201 pKa = 3.49 NWLDD205 pKa = 3.69 GEE207 pKa = 4.56 IEE209 pKa = 4.34 SFSIPGWGSWAKK221 pKa = 10.38 QNSKK225 pKa = 8.16 GTRR228 pKa = 11.84 LAGTAIANIMQKK240 pKa = 10.23 KK241 pKa = 10.53 DD242 pKa = 2.96 SDD244 pKa = 3.94 ALEE247 pKa = 4.15 KK248 pKa = 10.91 AKK250 pKa = 10.91 EE251 pKa = 4.13 KK252 pKa = 10.66 LEE254 pKa = 4.05 KK255 pKa = 9.58 MKK257 pKa = 9.63 KK258 pKa = 7.73 TADD261 pKa = 3.67 DD262 pKa = 4.01 PASLEE267 pKa = 4.2 SEE269 pKa = 4.28 GMSEE273 pKa = 4.22 DD274 pKa = 3.68 AAKK277 pKa = 10.59 KK278 pKa = 8.56 MCQEE282 pKa = 3.96 VEE284 pKa = 3.9 ASIEE288 pKa = 4.01 EE289 pKa = 4.33 AEE291 pKa = 4.41 VLLGTNVAQGEE302 pKa = 4.61 SKK304 pKa = 10.71 YY305 pKa = 9.53 QQQAAAMDD313 pKa = 4.24 VPFSAHH319 pKa = 3.7 YY320 pKa = 8.1 WAWRR324 pKa = 11.84 CKK326 pKa = 9.9 VNEE329 pKa = 4.01 NSFPALSQWLFEE341 pKa = 4.58 LGQRR345 pKa = 11.84 PIGAAKK351 pKa = 9.72 INTMLGEE358 pKa = 4.77 LPFIWARR365 pKa = 11.84 NIRR368 pKa = 11.84 GSFASSNFNGNKK380 pKa = 9.33 IYY382 pKa = 8.85 MHH384 pKa = 6.78 PAVLTAGRR392 pKa = 11.84 LSDD395 pKa = 3.39 MAACFGAFPVANPQRR410 pKa = 11.84 AAEE413 pKa = 4.3 GTGNTRR419 pKa = 11.84 YY420 pKa = 10.31 LLNLKK425 pKa = 10.41 RR426 pKa = 11.84 EE427 pKa = 4.03 GDD429 pKa = 3.88 NPCASMVTSLFDD441 pKa = 3.3 VFSAGFKK448 pKa = 9.06 YY449 pKa = 10.58 QDD451 pKa = 3.41 MDD453 pKa = 3.67 IVPPEE458 pKa = 4.16 HH459 pKa = 6.65 MLHH462 pKa = 6.36 QSFLGKK468 pKa = 9.1 TSPFQTANKK477 pKa = 9.66 IKK479 pKa = 10.79 GSFTKK484 pKa = 10.25 INVVAAPNKK493 pKa = 5.83 PTPYY497 pKa = 10.39 VFTPASAKK505 pKa = 10.08 KK506 pKa = 9.97 IKK508 pKa = 9.73 EE509 pKa = 3.89 KK510 pKa = 10.43 KK511 pKa = 9.15 ARR513 pKa = 11.84 KK514 pKa = 8.96
Molecular weight: 57.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.381
IPC2_protein 6.491
IPC_protein 6.576
Toseland 6.532
ProMoST 7.102
Dawson 7.073
Bjellqvist 7.015
Wikipedia 7.029
Rodwell 7.073
Grimsley 6.605
Solomon 7.102
Lehninger 7.117
Nozaki 7.337
DTASelect 7.337
Thurlkill 7.38
EMBOSS 7.395
Sillero 7.483
Patrickios 4.495
IPC_peptide 7.117
IPC2_peptide 6.81
IPC2.peptide.svr19 6.831
Protein with the highest isoelectric point:
>tr|A0A0B6VMT2|A0A0B6VMT2_9VIRU Glycoprotein G2 OS=Leopards Hill virus OX=1381104 GN=GP PE=4 SV=1
MM1 pKa = 6.81 EE2 pKa = 5.24 TYY4 pKa = 10.29 HH5 pKa = 6.68 GSRR8 pKa = 11.84 YY9 pKa = 7.43 TATSCLFFVIMLVVAITADD28 pKa = 3.58 NNVTEE33 pKa = 4.43 TTTAEE38 pKa = 4.36 VVSNTTGSTEE48 pKa = 4.16 GDD50 pKa = 3.67 TTSKK54 pKa = 11.15 GMDD57 pKa = 3.27 TTQIPQINQTSATTRR72 pKa = 11.84 SNNITTKK79 pKa = 9.21 TSRR82 pKa = 11.84 SEE84 pKa = 4.18 SGVDD88 pKa = 3.64 PSATQPLLVSSQASGTIGQQRR109 pKa = 11.84 GLSPTPDD116 pKa = 3.28 PGARR120 pKa = 11.84 IRR122 pKa = 11.84 VKK124 pKa = 10.11 GGKK127 pKa = 8.98 RR128 pKa = 11.84 RR129 pKa = 11.84 AEE131 pKa = 3.81 RR132 pKa = 11.84 LTARR136 pKa = 11.84 LDD138 pKa = 3.6 VNGNRR143 pKa = 11.84 VVLKK147 pKa = 10.78 RR148 pKa = 11.84 EE149 pKa = 3.59 ISGNLQLAKK158 pKa = 10.22 EE159 pKa = 4.05 QLEE162 pKa = 4.18 EE163 pKa = 3.97 AVLNMEE169 pKa = 4.75 IDD171 pKa = 3.86 SLDD174 pKa = 3.73 LEE176 pKa = 4.35 QLEE179 pKa = 4.57 EE180 pKa = 3.97 EE181 pKa = 4.29 SPYY184 pKa = 11.09 RR185 pKa = 11.84 SMQRR189 pKa = 11.84 TRR191 pKa = 11.84 LMLPKK196 pKa = 10.3 LEE198 pKa = 4.03 SFAYY202 pKa = 10.47 NEE204 pKa = 4.07 EE205 pKa = 4.03 QEE207 pKa = 4.25 EE208 pKa = 4.36 DD209 pKa = 3.92 VMVTGKK215 pKa = 10.62 LSRR218 pKa = 11.84 FTRR221 pKa = 11.84 FEE223 pKa = 4.43 RR224 pKa = 11.84 INIDD228 pKa = 3.15 KK229 pKa = 10.84 LEE231 pKa = 4.79 SITVFQNLTNRR242 pKa = 11.84 SVLTLCTYY250 pKa = 10.47 FGHH253 pKa = 6.63 YY254 pKa = 9.23 YY255 pKa = 10.93 NKK257 pKa = 8.65 TLKK260 pKa = 9.1 GTTVSIHH267 pKa = 6.1 HH268 pKa = 6.64 KK269 pKa = 10.52 CDD271 pKa = 3.71 IEE273 pKa = 4.48 TQCKK277 pKa = 10.05 GLTEE281 pKa = 4.23 HH282 pKa = 6.75 VSIDD286 pKa = 4.48 RR287 pKa = 11.84 ITNYY291 pKa = 11.17 DD292 pKa = 3.6 NIKK295 pKa = 9.9 IINIMEE301 pKa = 4.29 LPVIIIAYY309 pKa = 5.52 FTRR312 pKa = 11.84 HH313 pKa = 6.03 FGFTYY318 pKa = 10.11 KK319 pKa = 10.71 NLVIHH324 pKa = 6.14 EE325 pKa = 4.31 QIEE328 pKa = 4.41 NCLFFYY334 pKa = 9.82 PQNPVACMEE343 pKa = 4.41 NNWGGRR349 pKa = 11.84 SHH351 pKa = 7.13 PVVHH355 pKa = 6.57 LVIEE359 pKa = 4.62 RR360 pKa = 11.84 KK361 pKa = 9.12 YY362 pKa = 11.01 LKK364 pKa = 10.07 ADD366 pKa = 3.55 SSITLCGLKK375 pKa = 10.18 KK376 pKa = 10.78 GNPTSLKK383 pKa = 9.01 GTWFAEE389 pKa = 4.3 KK390 pKa = 10.06 TKK392 pKa = 10.04 VTIDD396 pKa = 3.12 LGEE399 pKa = 4.21 PQTAGRR405 pKa = 11.84 RR406 pKa = 11.84 KK407 pKa = 9.71 LLAIEE412 pKa = 4.09 RR413 pKa = 11.84 RR414 pKa = 11.84 RR415 pKa = 11.84 RR416 pKa = 11.84 LPGRR420 pKa = 11.84 EE421 pKa = 4.51 DD422 pKa = 3.21 IRR424 pKa = 11.84 CHH426 pKa = 6.73 SGSHH430 pKa = 5.66 LVKK433 pKa = 9.49 IDD435 pKa = 3.31 KK436 pKa = 9.64 YY437 pKa = 11.14 APTNEE442 pKa = 4.08 FQSFPNAKK450 pKa = 9.55 IGFCNDD456 pKa = 3.44 SIITHH461 pKa = 6.8 LPLGQEE467 pKa = 4.5 FGCYY471 pKa = 8.44 RR472 pKa = 11.84 VGSVKK477 pKa = 9.1 THH479 pKa = 5.11 VQCKK483 pKa = 8.49 PYY485 pKa = 10.28 HH486 pKa = 5.96 HH487 pKa = 7.11 AFDD490 pKa = 4.58 GEE492 pKa = 4.51 KK493 pKa = 8.84 TCNVSSDD500 pKa = 3.37 GDD502 pKa = 3.78 CRR504 pKa = 11.84 EE505 pKa = 4.39 GEE507 pKa = 3.82 LCAAVKK513 pKa = 10.82 LNGQGIITARR523 pKa = 11.84 THH525 pKa = 5.76 LGEE528 pKa = 4.27 VQVKK532 pKa = 9.81 HH533 pKa = 6.47 CLEE536 pKa = 4.08 EE537 pKa = 4.31 CQFSFTRR544 pKa = 11.84 VNDD547 pKa = 3.69 LEE549 pKa = 4.63 VSFTCPDD556 pKa = 3.15 GQQRR560 pKa = 11.84 RR561 pKa = 11.84 LHH563 pKa = 6.29 SNAVDD568 pKa = 3.88 TNCPFQNKK576 pKa = 9.62 LGVYY580 pKa = 9.9 ALYY583 pKa = 10.49 VCRR586 pKa = 11.84 ATHH589 pKa = 6.56 RR590 pKa = 11.84 PLVLYY595 pKa = 7.41 TTFIWFVVGTIVLATSLQMISVTVKK620 pKa = 9.1 LYY622 pKa = 10.79 CYY624 pKa = 10.31 LVVLAKK630 pKa = 10.47 RR631 pKa = 11.84 KK632 pKa = 9.88 LDD634 pKa = 3.51 TGKK637 pKa = 10.69 GVCTDD642 pKa = 3.48 CGEE645 pKa = 4.61 SVLSTEE651 pKa = 3.72 EE652 pKa = 3.98 WQRR655 pKa = 11.84 HH656 pKa = 3.89 QACKK660 pKa = 10.13 KK661 pKa = 9.93 GKK663 pKa = 9.93 CPYY666 pKa = 10.0 CGVKK670 pKa = 10.7 GSGNDD675 pKa = 3.52 VQKK678 pKa = 11.06 HH679 pKa = 5.39 VINCLQRR686 pKa = 11.84 EE687 pKa = 4.44 TVLEE691 pKa = 4.16 HH692 pKa = 7.1 DD693 pKa = 4.94 LNVLTIRR700 pKa = 11.84 RR701 pKa = 11.84 TPRR704 pKa = 11.84 YY705 pKa = 9.55 ALRR708 pKa = 11.84 LGCFLNALQGRR719 pKa = 11.84 PIRR722 pKa = 11.84 LIWLVVLLLLFVFLIKK738 pKa = 10.06 PVRR741 pKa = 11.84 SLEE744 pKa = 4.35 TNDD747 pKa = 3.17 QTEE750 pKa = 4.4 GLWEE754 pKa = 3.97 EE755 pKa = 4.77 GIEE758 pKa = 4.05 EE759 pKa = 4.28 VEE761 pKa = 4.39 KK762 pKa = 10.98 CGKK765 pKa = 9.29 GCWYY769 pKa = 10.25 NQDD772 pKa = 2.76 VCTCDD777 pKa = 3.53 KK778 pKa = 10.99 EE779 pKa = 5.0 EE780 pKa = 4.29 PLHH783 pKa = 5.4 TSRR786 pKa = 11.84 HH787 pKa = 5.15 ILSVPAKK794 pKa = 10.35 DD795 pKa = 3.75 DD796 pKa = 3.8 TEE798 pKa = 4.23 KK799 pKa = 10.62 ATSKK803 pKa = 10.56 DD804 pKa = 3.54 RR805 pKa = 11.84 QGKK808 pKa = 9.44 KK809 pKa = 8.0 VMRR812 pKa = 11.84 SLDD815 pKa = 3.51 VEE817 pKa = 4.55 APWGTLHH824 pKa = 7.41 IPEE827 pKa = 4.6 TFSPAGSVKK836 pKa = 10.22 HH837 pKa = 6.57 ISLSWEE843 pKa = 3.76 SSRR846 pKa = 11.84 VVGKK850 pKa = 10.33 RR851 pKa = 11.84 VILSGKK857 pKa = 8.1 STAILKK863 pKa = 9.37 LNPKK867 pKa = 9.24 TSTSWEE873 pKa = 4.19 MTSPDD878 pKa = 3.59 ANEE881 pKa = 4.15 KK882 pKa = 10.61 KK883 pKa = 10.38 ILTLSILDD891 pKa = 3.48 YY892 pKa = 9.52 TQIYY896 pKa = 9.59 SSRR899 pKa = 11.84 FEE901 pKa = 4.2 YY902 pKa = 9.88 LTGDD906 pKa = 3.48 RR907 pKa = 11.84 KK908 pKa = 9.28 VTTWSEE914 pKa = 4.65 GSCTGPCPKK923 pKa = 10.45 DD924 pKa = 3.65 CGCNDD929 pKa = 4.06 PSCHH933 pKa = 5.82 TKK935 pKa = 9.79 QWLNTRR941 pKa = 11.84 NWRR944 pKa = 11.84 CNPTWCWGIGTGCSCCSAKK963 pKa = 10.55 VVDD966 pKa = 5.93 LYY968 pKa = 11.7 KK969 pKa = 10.87 NWLVSIWQIEE979 pKa = 4.35 HH980 pKa = 6.57 LRR982 pKa = 11.84 TPVVACLEE990 pKa = 4.12 FDD992 pKa = 3.63 HH993 pKa = 6.81 EE994 pKa = 4.36 NRR996 pKa = 11.84 VCDD999 pKa = 3.71 VVEE1002 pKa = 4.37 AGIEE1006 pKa = 4.06 IQLGPVTVAFSDD1018 pKa = 3.62 PFGEE1022 pKa = 4.8 QKK1024 pKa = 10.41 ILPQRR1029 pKa = 11.84 IAVYY1033 pKa = 9.91 HH1034 pKa = 6.14 KK1035 pKa = 9.8 RR1036 pKa = 11.84 DD1037 pKa = 3.27 VDD1039 pKa = 3.8 HH1040 pKa = 6.68 EE1041 pKa = 4.41 HH1042 pKa = 6.97 VDD1044 pKa = 3.61 LLHH1047 pKa = 5.83 NHH1049 pKa = 7.44 GIGGAEE1055 pKa = 4.37 QYY1057 pKa = 10.68 CKK1059 pKa = 10.55 LQSCTHH1065 pKa = 5.87 GTAGDD1070 pKa = 3.8 YY1071 pKa = 10.87 QIINPDD1077 pKa = 3.41 ALVFDD1082 pKa = 6.57 DD1083 pKa = 4.28 ITSMNYY1089 pKa = 9.2 FKK1091 pKa = 11.17 KK1092 pKa = 10.57 LDD1094 pKa = 3.78 VYY1096 pKa = 11.36 NKK1098 pKa = 10.14 LWMSWEE1104 pKa = 4.09 GVNLGYY1110 pKa = 10.69 YY1111 pKa = 9.61 CNPGDD1116 pKa = 3.74 WTTCTAEE1123 pKa = 4.19 NIVVRR1128 pKa = 11.84 NSEE1131 pKa = 4.01 AFSNRR1136 pKa = 11.84 NNLEE1140 pKa = 3.75 RR1141 pKa = 11.84 NYY1143 pKa = 10.41 SVSHH1147 pKa = 6.7 FFHH1150 pKa = 7.04 SSRR1153 pKa = 11.84 VYY1155 pKa = 10.64 GSGKK1159 pKa = 10.02 SLVMDD1164 pKa = 4.22 LKK1166 pKa = 10.99 GRR1168 pKa = 11.84 PIQSGGNINVYY1179 pKa = 8.67 VTVNNLEE1186 pKa = 4.29 LNSKK1190 pKa = 10.11 KK1191 pKa = 10.75 VVITGLKK1198 pKa = 9.57 VNLRR1202 pKa = 11.84 ACTGCFGCNLGAEE1215 pKa = 4.58 CQISLSLTEE1224 pKa = 4.29 PDD1226 pKa = 4.02 EE1227 pKa = 4.22 FHH1229 pKa = 6.89 LHH1231 pKa = 6.06 LKK1233 pKa = 10.35 SVTPGVTVPDD1243 pKa = 3.5 TSFLVTSSEE1252 pKa = 3.59 EE1253 pKa = 3.56 KK1254 pKa = 9.77 MFNIRR1259 pKa = 11.84 VFSILKK1265 pKa = 9.92 DD1266 pKa = 3.45 VNFCIEE1272 pKa = 4.03 VLEE1275 pKa = 4.73 SKK1277 pKa = 10.31 HH1278 pKa = 7.21 CPEE1281 pKa = 5.16 CDD1283 pKa = 3.27 KK1284 pKa = 11.41 KK1285 pKa = 10.97 DD1286 pKa = 3.81 LQSCLQLTFEE1296 pKa = 4.71 DD1297 pKa = 4.38 PKK1299 pKa = 11.0 PVLLEE1304 pKa = 3.86 HH1305 pKa = 7.01 RR1306 pKa = 11.84 SVLFSKK1312 pKa = 10.84 SNQTCGDD1319 pKa = 3.61 STISCWSSSAGILFKK1334 pKa = 11.2 GIGNFLSTHH1343 pKa = 6.52 FGSIFRR1349 pKa = 11.84 GILLSVLPILLIVGLIFFSPQIISLMRR1376 pKa = 11.84 LCKK1379 pKa = 9.99 RR1380 pKa = 11.84 GRR1382 pKa = 11.84 SVVGFRR1388 pKa = 11.84 KK1389 pKa = 9.92 RR1390 pKa = 11.84 FYY1392 pKa = 11.13 KK1393 pKa = 10.55 PLTDD1397 pKa = 3.9 GADD1400 pKa = 3.1 MGLSAEE1406 pKa = 4.39 EE1407 pKa = 4.24 KK1408 pKa = 10.82 AFLTGIFGKK1417 pKa = 10.53 KK1418 pKa = 9.5 KK1419 pKa = 9.51 EE1420 pKa = 4.07
Molecular weight: 159.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.976
IPC2_protein 7.263
IPC_protein 7.015
Toseland 6.722
ProMoST 7.658
Dawson 7.732
Bjellqvist 8.258
Wikipedia 7.629
Rodwell 7.761
Grimsley 6.795
Solomon 7.732
Lehninger 7.761
Nozaki 8.595
DTASelect 7.922
Thurlkill 7.98
EMBOSS 7.951
Sillero 8.331
Patrickios 4.126
IPC_peptide 7.732
IPC2_peptide 7.454
IPC2.peptide.svr19 7.225
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
5901
514
3967
1967.0
222.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.118 ± 1.254
2.61 ± 0.596
5.05 ± 0.309
7.202 ± 0.367
4.203 ± 0.135
5.287 ± 0.895
2.135 ± 0.359
5.965 ± 0.262
7.541 ± 0.519
10.185 ± 1.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.339 ± 0.433
5.118 ± 0.479
3.474 ± 0.224
3.474 ± 0.024
4.931 ± 0.408
8.863 ± 0.678
5.626 ± 0.878
6.779 ± 0.44
1.288 ± 0.364
2.813 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here