Raspberry leaf mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0MBW5|A0MBW5_9CLOS p7 OS=Raspberry leaf mottle virus OX=326941 PE=4 SV=1
SS1 pKa = 6.97YY2 pKa = 10.13IRR4 pKa = 11.84SQAIPPRR11 pKa = 11.84RR12 pKa = 11.84PSLQEE17 pKa = 3.47NLLSYY22 pKa = 10.25EE23 pKa = 3.78SRR25 pKa = 11.84NFNFIKK31 pKa = 10.44CEE33 pKa = 3.82RR34 pKa = 11.84FSSPKK39 pKa = 10.53LFGAAMASNLLLKK52 pKa = 10.91LFDD55 pKa = 4.0AEE57 pKa = 4.17KK58 pKa = 10.19LAEE61 pKa = 4.11VRR63 pKa = 11.84QSVISISEE71 pKa = 3.91ANIAKK76 pKa = 9.48WLLKK80 pKa = 10.13RR81 pKa = 11.84DD82 pKa = 3.61ASQVKK87 pKa = 10.48ALMTDD92 pKa = 3.55LDD94 pKa = 4.26RR95 pKa = 11.84DD96 pKa = 3.8FDD98 pKa = 4.02IMDD101 pKa = 5.19DD102 pKa = 3.28ISRR105 pKa = 11.84FKK107 pKa = 11.5LMVKK111 pKa = 9.85RR112 pKa = 11.84DD113 pKa = 3.9AKK115 pKa = 11.12VKK117 pKa = 10.8LDD119 pKa = 3.84DD120 pKa = 4.14SCLSKK125 pKa = 10.87HH126 pKa = 6.29PPAQNIMFHH135 pKa = 6.67RR136 pKa = 11.84KK137 pKa = 9.01ALNAVYY143 pKa = 10.1SPCFDD148 pKa = 3.32EE149 pKa = 4.99FKK151 pKa = 11.34NRR153 pKa = 11.84FLYY156 pKa = 10.05CLPPNIVFFTEE167 pKa = 3.89MTNEE171 pKa = 3.86DD172 pKa = 3.45LAEE175 pKa = 4.68IIRR178 pKa = 11.84RR179 pKa = 11.84RR180 pKa = 11.84LGDD183 pKa = 3.71DD184 pKa = 4.04DD185 pKa = 4.41IYY187 pKa = 11.5NIGEE191 pKa = 3.91VDD193 pKa = 4.24FSKK196 pKa = 10.63FDD198 pKa = 3.25KK199 pKa = 11.09SQDD202 pKa = 3.58VFIKK206 pKa = 9.85EE207 pKa = 4.09YY208 pKa = 10.88EE209 pKa = 3.99RR210 pKa = 11.84ALYY213 pKa = 9.66EE214 pKa = 3.96AFGFDD219 pKa = 4.45VEE221 pKa = 4.36LLEE224 pKa = 4.01MWMEE228 pKa = 4.34GEE230 pKa = 4.24YY231 pKa = 10.61NAYY234 pKa = 10.27ASTMDD239 pKa = 3.27SQLSFRR245 pKa = 11.84IEE247 pKa = 3.71NQRR250 pKa = 11.84RR251 pKa = 11.84SGGSNTWIGNSLVTLGLLSMYY272 pKa = 10.64YY273 pKa = 10.2DD274 pKa = 3.38VSKK277 pKa = 10.82FRR279 pKa = 11.84LLLISGDD286 pKa = 3.76DD287 pKa = 3.44SLIYY291 pKa = 10.35SDD293 pKa = 5.55EE294 pKa = 4.36KK295 pKa = 11.09IKK297 pKa = 10.98DD298 pKa = 3.57HH299 pKa = 7.06SSQICLEE306 pKa = 4.29TGFEE310 pKa = 4.48TKK312 pKa = 10.05FLSPSVPYY320 pKa = 9.82FCSKK324 pKa = 10.42FVVQTGSMTYY334 pKa = 9.62FVPDD338 pKa = 4.12PYY340 pKa = 11.41KK341 pKa = 10.99LLVKK345 pKa = 10.63LGGSTPFVTDD355 pKa = 2.64VDD357 pKa = 4.06LFEE360 pKa = 5.86AFVSFRR366 pKa = 11.84DD367 pKa = 3.37LTRR370 pKa = 11.84AFDD373 pKa = 3.67HH374 pKa = 6.56QVMLEE379 pKa = 4.02RR380 pKa = 11.84LCGLVHH386 pKa = 6.61TKK388 pKa = 10.69YY389 pKa = 10.61CFTSGSTLPALCAIHH404 pKa = 7.2CIRR407 pKa = 11.84ANFSSFKK414 pKa = 10.31KK415 pKa = 10.42LYY417 pKa = 9.11PKK419 pKa = 9.68TVGWWLVTSSRR430 pKa = 11.84LKK432 pKa = 10.67FLSKK436 pKa = 10.83LPGLIVSKK444 pKa = 10.67AFSSTGEE451 pKa = 4.15SNYY454 pKa = 9.89FCYY457 pKa = 10.61LRR459 pKa = 11.84DD460 pKa = 3.78SFADD464 pKa = 3.69DD465 pKa = 3.9PGG467 pKa = 4.76

Molecular weight: 53.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0MBW6|A0MBW6_9CLOS p6 OS=Raspberry leaf mottle virus OX=326941 PE=4 SV=1
MM1 pKa = 8.18PITEE5 pKa = 4.41SEE7 pKa = 4.75TPFSLAASPSSPPLRR22 pKa = 11.84STRR25 pKa = 11.84VPKK28 pKa = 10.68SSILAFSLLLMLTLCLISAFFISCFRR54 pKa = 11.84FHH56 pKa = 7.27RR57 pKa = 11.84FCRR60 pKa = 11.84LL61 pKa = 2.93

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5318

51

2819

531.8

59.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.826 ± 0.537

2.219 ± 0.194

5.792 ± 0.288

4.494 ± 0.44

5.923 ± 0.655

5.491 ± 0.376

2.144 ± 0.14

4.87 ± 0.638

5.096 ± 0.406

10.361 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.636 ± 0.417

3.291 ± 0.316

4.964 ± 0.361

1.824 ± 0.155

6.074 ± 0.465

10.624 ± 0.782

5.716 ± 0.62

8.274 ± 0.728

0.696 ± 0.113

3.686 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski