Gompholobium virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; unclassified Tombusviridae

Average proteome isoelectric point is 7.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2ARX5|A0A1B2ARX5_9TOMB Isoform of A0A1B2ARY9 Replication-associated protein OS=Gompholobium virus A OX=1884832 GN=RdRP PE=4 SV=1
MM1 pKa = 7.58EE2 pKa = 5.46RR3 pKa = 11.84PNLHH7 pKa = 5.37VTLVAAIVISLLLLNQYY24 pKa = 9.08PLQILSIFRR33 pKa = 11.84GNWSHH38 pKa = 7.12EE39 pKa = 3.85ILEE42 pKa = 4.78GIIVLACSFLLATACSTHH60 pKa = 5.89TPSYY64 pKa = 10.8SYY66 pKa = 11.71SNYY69 pKa = 9.6CDD71 pKa = 3.27NSKK74 pKa = 8.08TQHH77 pKa = 6.0IAISTGGG84 pKa = 3.23

Molecular weight: 9.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2ARY9|A0A1B2ARY9_9TOMB RNA-directed RNA polymerase OS=Gompholobium virus A OX=1884832 GN=RdRP PE=4 SV=1
MM1 pKa = 7.61EE2 pKa = 5.37SDD4 pKa = 3.56KK5 pKa = 11.19PVNKK9 pKa = 10.06ALKK12 pKa = 10.35KK13 pKa = 10.15EE14 pKa = 4.02VGEE17 pKa = 4.17NEE19 pKa = 4.01RR20 pKa = 11.84KK21 pKa = 7.95NTGRR25 pKa = 11.84RR26 pKa = 11.84AVSRR30 pKa = 11.84DD31 pKa = 3.13VARR34 pKa = 11.84TGTTKK39 pKa = 10.54FARR42 pKa = 11.84DD43 pKa = 3.59TGSGNCNFIIIAEE56 pKa = 4.38SVSITNTFHH65 pKa = 7.19FF66 pKa = 4.88

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1531

66

777

306.2

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.054 ± 0.536

2.613 ± 0.231

4.246 ± 0.238

5.748 ± 0.768

4.18 ± 0.367

6.662 ± 0.334

2.939 ± 0.807

5.291 ± 0.568

6.466 ± 0.532

8.491 ± 1.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.743 ± 0.237

3.984 ± 0.504

4.703 ± 0.293

3.005 ± 0.361

5.552 ± 0.627

7.773 ± 0.949

6.597 ± 1.757

7.12 ± 0.511

1.437 ± 0.229

3.396 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski