Amino acid dipepetide frequency for Pusillimonas sp. T2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.421AlaAla: 13.421 ± 0.153	1.186AlaCys: 1.186 ± 0.039	6.186AlaAsp: 6.186 ± 0.096	5.736AlaGlu: 5.736 ± 0.101	3.884AlaPhe: 3.884 ± 0.067	9.093AlaGly: 9.093 ± 0.101	2.509AlaHis: 2.509 ± 0.055	5.715AlaIle: 5.715 ± 0.081	3.677AlaLys: 3.677 ± 0.077	13.196AlaLeu: 13.196 ± 0.145	3.056AlaMet: 3.056 ± 0.062	3.282AlaAsn: 3.282 ± 0.061	4.94AlaPro: 4.94 ± 0.085	5.821AlaGln: 5.821 ± 0.085	7.146AlaArg: 7.146 ± 0.103	6.124AlaSer: 6.124 ± 0.1	5.563AlaThr: 5.563 ± 0.085	8.103AlaVal: 8.103 ± 0.096	1.655AlaTrp: 1.655 ± 0.046	2.635AlaTyr: 2.635 ± 0.055	0.001AlaXaa: 0.001 ± 0.001
Cys	0.977CysAla: 0.977 ± 0.031	0.131CysCys: 0.131 ± 0.012	0.529CysAsp: 0.529 ± 0.027	0.459CysGlu: 0.459 ± 0.025	0.338CysPhe: 0.338 ± 0.017	0.927CysGly: 0.927 ± 0.032	0.268CysHis: 0.268 ± 0.018	0.466CysIle: 0.466 ± 0.023	0.246CysLys: 0.246 ± 0.016	0.93CysLeu: 0.93 ± 0.036	0.219CysMet: 0.219 ± 0.014	0.27CysAsn: 0.27 ± 0.016	0.529CysPro: 0.529 ± 0.024	0.32CysGln: 0.32 ± 0.017	0.564CysArg: 0.564 ± 0.022	0.473CysSer: 0.473 ± 0.024	0.52CysThr: 0.52 ± 0.022	0.759CysVal: 0.759 ± 0.028	0.114CysTrp: 0.114 ± 0.011	0.22CysTyr: 0.22 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.384AspAla: 6.384 ± 0.087	0.483AspCys: 0.483 ± 0.023	3.25AspAsp: 3.25 ± 0.084	3.175AspGlu: 3.175 ± 0.057	2.059AspPhe: 2.059 ± 0.052	4.039AspGly: 4.039 ± 0.06	1.173AspHis: 1.173 ± 0.037	3.36AspIle: 3.36 ± 0.063	1.798AspLys: 1.798 ± 0.043	5.466AspLeu: 5.466 ± 0.084	1.3AspMet: 1.3 ± 0.034	1.597AspAsn: 1.597 ± 0.044	2.955AspPro: 2.955 ± 0.054	2.152AspGln: 2.152 ± 0.048	3.272AspArg: 3.272 ± 0.059	2.386AspSer: 2.386 ± 0.05	3.137AspThr: 3.137 ± 0.065	4.124AspVal: 4.124 ± 0.072	0.943AspTrp: 0.943 ± 0.03	1.6AspTyr: 1.6 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	5.927GluAla: 5.927 ± 0.085	0.395GluCys: 0.395 ± 0.021	2.316GluAsp: 2.316 ± 0.044	2.335GluGlu: 2.335 ± 0.054	1.794GluPhe: 1.794 ± 0.048	3.725GluGly: 3.725 ± 0.071	1.397GluHis: 1.397 ± 0.044	2.951GluIle: 2.951 ± 0.055	2.188GluLys: 2.188 ± 0.053	5.408GluLeu: 5.408 ± 0.075	1.297GluMet: 1.297 ± 0.037	1.749GluAsn: 1.749 ± 0.044	2.49GluPro: 2.49 ± 0.058	2.861GluGln: 2.861 ± 0.059	4.022GluArg: 4.022 ± 0.075	2.837GluSer: 2.837 ± 0.053	2.894GluThr: 2.894 ± 0.061	3.691GluVal: 3.691 ± 0.063	0.674GluTrp: 0.674 ± 0.027	1.205GluTyr: 1.205 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	3.744PheAla: 3.744 ± 0.061	0.41PheCys: 0.41 ± 0.023	2.591PheAsp: 2.591 ± 0.054	2.191PheGlu: 2.191 ± 0.047	1.395PhePhe: 1.395 ± 0.047	3.338PheGly: 3.338 ± 0.066	0.684PheHis: 0.684 ± 0.026	1.916PheIle: 1.916 ± 0.047	1.296PheLys: 1.296 ± 0.041	3.203PheLeu: 3.203 ± 0.075	0.916PheMet: 0.916 ± 0.028	1.334PheAsn: 1.334 ± 0.038	1.513PhePro: 1.513 ± 0.038	1.149PheGln: 1.149 ± 0.028	1.807PheArg: 1.807 ± 0.038	2.485PheSer: 2.485 ± 0.052	2.052PheThr: 2.052 ± 0.063	2.806PheVal: 2.806 ± 0.057	0.56PheTrp: 0.56 ± 0.03	1.049PheTyr: 1.049 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	7.737GlyAla: 7.737 ± 0.116	0.823GlyCys: 0.823 ± 0.029	3.675GlyAsp: 3.675 ± 0.085	3.743GlyGlu: 3.743 ± 0.071	3.105GlyPhe: 3.105 ± 0.058	5.965GlyGly: 5.965 ± 0.097	1.919GlyHis: 1.919 ± 0.045	4.324GlyIle: 4.324 ± 0.071	3.673GlyLys: 3.673 ± 0.063	8.9GlyLeu: 8.9 ± 0.113	2.21GlyMet: 2.21 ± 0.05	2.423GlyAsn: 2.423 ± 0.068	3.047GlyPro: 3.047 ± 0.06	3.671GlyGln: 3.671 ± 0.06	4.835GlyArg: 4.835 ± 0.069	4.348GlySer: 4.348 ± 0.091	4.078GlyThr: 4.078 ± 0.101	6.47GlyVal: 6.47 ± 0.077	1.252GlyTrp: 1.252 ± 0.035	2.358GlyTyr: 2.358 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	2.525HisAla: 2.525 ± 0.056	0.267HisCys: 0.267 ± 0.017	1.402HisAsp: 1.402 ± 0.046	1.176HisGlu: 1.176 ± 0.036	0.9HisPhe: 0.9 ± 0.036	1.869HisGly: 1.869 ± 0.046	0.712HisHis: 0.712 ± 0.031	1.262HisIle: 1.262 ± 0.035	0.651HisLys: 0.651 ± 0.024	2.175HisLeu: 2.175 ± 0.052	0.549HisMet: 0.549 ± 0.024	0.674HisAsn: 0.674 ± 0.029	1.44HisPro: 1.44 ± 0.039	0.83HisGln: 0.83 ± 0.029	1.378HisArg: 1.378 ± 0.038	1.049HisSer: 1.049 ± 0.032	1.206HisThr: 1.206 ± 0.039	1.566HisVal: 1.566 ± 0.041	0.402HisTrp: 0.402 ± 0.021	0.721HisTyr: 0.721 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.934IleAla: 5.934 ± 0.082	0.493IleCys: 0.493 ± 0.023	3.692IleAsp: 3.692 ± 0.059	3.584IleGlu: 3.584 ± 0.067	1.641IlePhe: 1.641 ± 0.043	4.347IleGly: 4.347 ± 0.065	0.983IleHis: 0.983 ± 0.03	2.387IleIle: 2.387 ± 0.052	1.979IleLys: 1.979 ± 0.043	4.184IleLeu: 4.184 ± 0.079	1.153IleMet: 1.153 ± 0.036	1.918IleAsn: 1.918 ± 0.045	2.327IlePro: 2.327 ± 0.052	1.728IleGln: 1.728 ± 0.042	2.901IleArg: 2.901 ± 0.055	3.154IleSer: 3.154 ± 0.062	3.002IleThr: 3.002 ± 0.077	3.902IleVal: 3.902 ± 0.07	0.611IleTrp: 0.611 ± 0.029	1.219IleTyr: 1.219 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.289LysAla: 4.289 ± 0.077	0.201LysCys: 0.201 ± 0.015	1.667LysAsp: 1.667 ± 0.049	1.732LysGlu: 1.732 ± 0.043	1.072LysPhe: 1.072 ± 0.036	2.723LysGly: 2.723 ± 0.052	0.789LysHis: 0.789 ± 0.024	1.801LysIle: 1.801 ± 0.046	1.617LysLys: 1.617 ± 0.045	3.426LysLeu: 3.426 ± 0.065	0.921LysMet: 0.921 ± 0.03	1.248LysAsn: 1.248 ± 0.036	2.205LysPro: 2.205 ± 0.047	1.527LysGln: 1.527 ± 0.042	2.278LysArg: 2.278 ± 0.052	2.002LysSer: 2.002 ± 0.047	2.257LysThr: 2.257 ± 0.049	2.824LysVal: 2.824 ± 0.055	0.426LysTrp: 0.426 ± 0.021	0.787LysTyr: 0.787 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	12.91LeuAla: 12.91 ± 0.138	1.003LeuCys: 1.003 ± 0.034	5.898LeuAsp: 5.898 ± 0.082	5.389LeuGlu: 5.389 ± 0.066	3.758LeuPhe: 3.758 ± 0.071	8.272LeuGly: 8.272 ± 0.105	2.204LeuHis: 2.204 ± 0.052	5.226LeuIle: 5.226 ± 0.087	4.083LeuLys: 4.083 ± 0.058	10.582LeuLeu: 10.582 ± 0.158	2.623LeuMet: 2.623 ± 0.06	3.398LeuAsn: 3.398 ± 0.051	5.601LeuPro: 5.601 ± 0.09	4.043LeuGln: 4.043 ± 0.076	6.632LeuArg: 6.632 ± 0.086	6.874LeuSer: 6.874 ± 0.092	5.782LeuThr: 5.782 ± 0.085	7.889LeuVal: 7.889 ± 0.112	1.302LeuTrp: 1.302 ± 0.048	2.272LeuTyr: 2.272 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	3.037MetAla: 3.037 ± 0.057	0.193MetCys: 0.193 ± 0.015	0.973MetAsp: 0.973 ± 0.035	0.867MetGlu: 0.867 ± 0.027	0.788MetPhe: 0.788 ± 0.028	1.938MetGly: 1.938 ± 0.052	0.559MetHis: 0.559 ± 0.027	1.087MetIle: 1.087 ± 0.034	0.986MetLys: 0.986 ± 0.035	2.833MetLeu: 2.833 ± 0.055	0.634MetMet: 0.634 ± 0.029	0.843MetAsn: 0.843 ± 0.029	1.469MetPro: 1.469 ± 0.039	1.157MetGln: 1.157 ± 0.037	1.694MetArg: 1.694 ± 0.041	1.813MetSer: 1.813 ± 0.044	1.726MetThr: 1.726 ± 0.043	1.804MetVal: 1.804 ± 0.039	0.206MetTrp: 0.206 ± 0.016	0.421MetTyr: 0.421 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.664AsnAla: 3.664 ± 0.076	0.266AsnCys: 0.266 ± 0.016	1.773AsnAsp: 1.773 ± 0.054	1.643AsnGlu: 1.643 ± 0.046	1.049AsnPhe: 1.049 ± 0.036	2.614AsnGly: 2.614 ± 0.063	0.685AsnHis: 0.685 ± 0.023	1.747AsnIle: 1.747 ± 0.04	1.063AsnLys: 1.063 ± 0.034	3.023AsnLeu: 3.023 ± 0.054	0.76AsnMet: 0.76 ± 0.028	1.087AsnAsn: 1.087 ± 0.046	2.15AsnPro: 2.15 ± 0.047	1.241AsnGln: 1.241 ± 0.041	1.812AsnArg: 1.812 ± 0.041	1.464AsnSer: 1.464 ± 0.041	1.915AsnThr: 1.915 ± 0.05	2.374AsnVal: 2.374 ± 0.053	0.453AsnTrp: 0.453 ± 0.025	0.811AsnTyr: 0.811 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	5.715ProAla: 5.715 ± 0.09	0.352ProCys: 0.352 ± 0.019	3.624ProAsp: 3.624 ± 0.062	3.694ProGlu: 3.694 ± 0.065	1.829ProPhe: 1.829 ± 0.043	4.03ProGly: 4.03 ± 0.064	1.056ProHis: 1.056 ± 0.03	2.172ProIle: 2.172 ± 0.052	1.698ProLys: 1.698 ± 0.046	4.765ProLeu: 4.765 ± 0.081	1.219ProMet: 1.219 ± 0.032	1.627ProAsn: 1.627 ± 0.043	2.144ProPro: 2.144 ± 0.053	2.0ProGln: 2.0 ± 0.049	2.353ProArg: 2.353 ± 0.05	2.744ProSer: 2.744 ± 0.054	2.523ProThr: 2.523 ± 0.049	4.417ProVal: 4.417 ± 0.061	0.75ProTrp: 0.75 ± 0.027	1.29ProTyr: 1.29 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.982GlnAla: 5.982 ± 0.087	0.362GlnCys: 0.362 ± 0.018	1.809GlnAsp: 1.809 ± 0.044	1.813GlnGlu: 1.813 ± 0.046	1.514GlnPhe: 1.514 ± 0.037	3.2GlnGly: 3.2 ± 0.055	1.06GlnHis: 1.06 ± 0.033	2.075GlnIle: 2.075 ± 0.04	1.409GlnLys: 1.409 ± 0.04	4.409GlnLeu: 4.409 ± 0.074	1.094GlnMet: 1.094 ± 0.038	1.243GlnAsn: 1.243 ± 0.036	2.183GlnPro: 2.183 ± 0.051	2.098GlnGln: 2.098 ± 0.047	3.13GlnArg: 3.13 ± 0.066	2.44GlnSer: 2.44 ± 0.044	2.404GlnThr: 2.404 ± 0.051	3.337GlnVal: 3.337 ± 0.059	0.785GlnTrp: 0.785 ± 0.026	0.981GlnTyr: 0.981 ± 0.038	0.001GlnXaa: 0.001 ± 0.001
Arg	6.243ArgAla: 6.243 ± 0.077	0.555ArgCys: 0.555 ± 0.026	3.237ArgAsp: 3.237 ± 0.059	3.436ArgGlu: 3.436 ± 0.066	2.538ArgPhe: 2.538 ± 0.043	3.807ArgGly: 3.807 ± 0.064	1.746ArgHis: 1.746 ± 0.052	3.35ArgIle: 3.35 ± 0.061	2.195ArgLys: 2.195 ± 0.044	7.327ArgLeu: 7.327 ± 0.107	1.615ArgMet: 1.615 ± 0.036	2.016ArgAsn: 2.016 ± 0.045	2.814ArgPro: 2.814 ± 0.056	3.16ArgGln: 3.16 ± 0.062	4.321ArgArg: 4.321 ± 0.067	3.28ArgSer: 3.28 ± 0.063	2.941ArgThr: 2.941 ± 0.056	4.783ArgVal: 4.783 ± 0.073	1.011ArgTrp: 1.011 ± 0.035	1.971ArgTyr: 1.971 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.204SerAla: 6.204 ± 0.089	0.474SerCys: 0.474 ± 0.025	2.878SerAsp: 2.878 ± 0.053	2.621SerGlu: 2.621 ± 0.054	2.27SerPhe: 2.27 ± 0.054	5.274SerGly: 5.274 ± 0.092	1.259SerHis: 1.259 ± 0.034	2.88SerIle: 2.88 ± 0.062	1.733SerLys: 1.733 ± 0.041	6.057SerLeu: 6.057 ± 0.083	1.515SerMet: 1.515 ± 0.036	1.723SerAsn: 1.723 ± 0.047	2.901SerPro: 2.901 ± 0.057	2.36SerGln: 2.36 ± 0.056	3.556SerArg: 3.556 ± 0.056	3.54SerSer: 3.54 ± 0.081	3.141SerThr: 3.141 ± 0.073	4.602SerVal: 4.602 ± 0.066	0.756SerTrp: 0.756 ± 0.032	1.379SerTyr: 1.379 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.539ThrAla: 5.539 ± 0.088	0.417ThrCys: 0.417 ± 0.021	2.887ThrAsp: 2.887 ± 0.054	2.768ThrGlu: 2.768 ± 0.058	1.869ThrPhe: 1.869 ± 0.069	4.656ThrGly: 4.656 ± 0.103	1.29ThrHis: 1.29 ± 0.034	2.586ThrIle: 2.586 ± 0.084	1.429ThrLys: 1.429 ± 0.037	6.815ThrLeu: 6.815 ± 0.096	1.074ThrMet: 1.074 ± 0.037	1.467ThrAsn: 1.467 ± 0.042	3.581ThrPro: 3.581 ± 0.062	2.448ThrGln: 2.448 ± 0.047	3.263ThrArg: 3.263 ± 0.064	2.945ThrSer: 2.945 ± 0.061	3.009ThrThr: 3.009 ± 0.079	4.276ThrVal: 4.276 ± 0.105	0.717ThrTrp: 0.717 ± 0.029	1.273ThrTyr: 1.273 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	8.728ValAla: 8.728 ± 0.087	0.81ValCys: 0.81 ± 0.031	4.234ValAsp: 4.234 ± 0.075	3.831ValGlu: 3.831 ± 0.057	2.962ValPhe: 2.962 ± 0.063	5.727ValGly: 5.727 ± 0.08	1.52ValHis: 1.52 ± 0.041	4.099ValIle: 4.099 ± 0.065	2.798ValLys: 2.798 ± 0.057	8.393ValLeu: 8.393 ± 0.109	1.967ValMet: 1.967 ± 0.04	2.564ValAsn: 2.564 ± 0.053	3.718ValPro: 3.718 ± 0.053	2.948ValGln: 2.948 ± 0.065	4.546ValArg: 4.546 ± 0.076	4.979ValSer: 4.979 ± 0.074	4.146ValThr: 4.146 ± 0.074	6.65ValVal: 6.65 ± 0.105	0.993ValTrp: 0.993 ± 0.04	1.855ValTyr: 1.855 ± 0.045	0.001ValXaa: 0.001 ± 0.001
Trp	1.327TrpAla: 1.327 ± 0.039	0.157TrpCys: 0.157 ± 0.012	0.564TrpAsp: 0.564 ± 0.024	0.593TrpGlu: 0.593 ± 0.025	0.609TrpPhe: 0.609 ± 0.023	0.854TrpGly: 0.854 ± 0.03	0.418TrpHis: 0.418 ± 0.02	0.615TrpIle: 0.615 ± 0.029	0.443TrpLys: 0.443 ± 0.02	2.073TrpLeu: 2.073 ± 0.058	0.395TrpMet: 0.395 ± 0.021	0.378TrpAsn: 0.378 ± 0.021	0.712TrpPro: 0.712 ± 0.03	0.806TrpGln: 0.806 ± 0.029	1.108TrpArg: 1.108 ± 0.038	0.814TrpSer: 0.814 ± 0.029	0.597TrpThr: 0.597 ± 0.022	1.204TrpVal: 1.204 ± 0.039	0.239TrpTrp: 0.239 ± 0.016	0.352TrpTyr: 0.352 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.642TyrAla: 2.642 ± 0.052	0.278TyrCys: 0.278 ± 0.018	1.468TyrAsp: 1.468 ± 0.038	1.37TyrGlu: 1.37 ± 0.038	1.067TyrPhe: 1.067 ± 0.038	2.158TyrGly: 2.158 ± 0.052	0.522TyrHis: 0.522 ± 0.023	1.116TyrIle: 1.116 ± 0.032	0.832TyrLys: 0.832 ± 0.032	2.577TyrLeu: 2.577 ± 0.048	0.512TyrMet: 0.512 ± 0.024	0.753TyrAsn: 0.753 ± 0.027	1.28TyrPro: 1.28 ± 0.039	1.009TyrGln: 1.009 ± 0.038	1.725TyrArg: 1.725 ± 0.042	1.374TyrSer: 1.374 ± 0.036	1.418TyrThr: 1.418 ± 0.033	1.887TyrVal: 1.887 ± 0.043	0.411TyrTrp: 0.411 ± 0.02	0.71TyrTyr: 0.71 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3054 proteins (1004463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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