Gemmobacter sp. HYN0069

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Gemmobacter; unclassified Gemmobacter

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S0UL48|A0A2S0UL48_9RHOB Oxidoreductase OS=Gemmobacter sp. HYN0069 OX=2169400 GN=HYN69_08385 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 10.43HH3 pKa = 6.32SILVSALMLGASSLPSVAFDD23 pKa = 3.98FGNGLSATGEE33 pKa = 4.09VEE35 pKa = 4.44IEE37 pKa = 4.13YY38 pKa = 10.56FDD40 pKa = 5.56ADD42 pKa = 3.75GDD44 pKa = 4.5SEE46 pKa = 4.32TLAYY50 pKa = 10.35GSADD54 pKa = 3.2LTFRR58 pKa = 11.84QPGGAFGGFIGLDD71 pKa = 3.82AIAISGDD78 pKa = 3.72SEE80 pKa = 5.43SIFYY84 pKa = 10.55GALTYY89 pKa = 9.8TADD92 pKa = 3.94FGNIQIGAPRR102 pKa = 11.84PVVDD106 pKa = 5.64DD107 pKa = 4.17YY108 pKa = 12.05FDD110 pKa = 4.19APVIGGARR118 pKa = 11.84IVDD121 pKa = 4.99LIVPVGRR128 pKa = 11.84SFRR131 pKa = 11.84STILTLGGNLDD142 pKa = 3.69TPAGIRR148 pKa = 11.84YY149 pKa = 9.89DD150 pKa = 3.61GTFGNLQAGASYY162 pKa = 11.32NRR164 pKa = 11.84FDD166 pKa = 4.41GGDD169 pKa = 3.64IIDD172 pKa = 3.83AAARR176 pKa = 11.84LNLDD180 pKa = 3.5TVSFSGSVEE189 pKa = 3.86RR190 pKa = 11.84LADD193 pKa = 4.26DD194 pKa = 4.57GNFAQTIYY202 pKa = 11.08NIGAEE207 pKa = 3.91ARR209 pKa = 11.84LDD211 pKa = 3.89KK212 pKa = 10.66LTMGAIYY219 pKa = 9.78TSLGMFDD226 pKa = 3.8QSATQIYY233 pKa = 10.06AIYY236 pKa = 10.55SLVEE240 pKa = 3.99NLDD243 pKa = 3.6LAASYY248 pKa = 9.98MVFDD252 pKa = 4.28SAFGGDD258 pKa = 3.62DD259 pKa = 4.11AFGLSAKK266 pKa = 10.6YY267 pKa = 10.64GFTNGLYY274 pKa = 10.5AEE276 pKa = 4.92AGFSEE281 pKa = 5.01GVISDD286 pKa = 4.05EE287 pKa = 5.28LYY289 pKa = 8.71TASVGISFF297 pKa = 5.06

Molecular weight: 31.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S0UI51|A0A2S0UI51_9RHOB Acyl_transf_3 domain-containing protein OS=Gemmobacter sp. HYN0069 OX=2169400 GN=HYN69_02215 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3779

0

3779

1180242

29

6463

312.3

33.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.519 ± 0.065

0.866 ± 0.013

5.853 ± 0.059

5.235 ± 0.034

3.641 ± 0.027

9.11 ± 0.085

1.987 ± 0.022

4.765 ± 0.03

2.957 ± 0.032

10.229 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.716 ± 0.023

2.387 ± 0.027

5.139 ± 0.034

2.975 ± 0.021

6.871 ± 0.044

4.965 ± 0.038

5.636 ± 0.042

7.659 ± 0.036

1.412 ± 0.018

2.079 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski