Streptococcus phage Javan406

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B258|A0A4D6B258_9CAUD Uncharacterized protein OS=Streptococcus phage Javan406 OX=2548152 GN=Javan406_0051 PE=4 SV=1
MM1 pKa = 7.29PQAINGKK8 pKa = 9.54DD9 pKa = 3.03LLAFFRR15 pKa = 11.84RR16 pKa = 11.84FADD19 pKa = 3.69RR20 pKa = 11.84ATVDD24 pKa = 2.82ARR26 pKa = 11.84RR27 pKa = 11.84NRR29 pKa = 11.84FQTEE33 pKa = 4.02HH34 pKa = 5.62TVSAEE39 pKa = 3.85KK40 pKa = 9.31EE41 pKa = 4.64TNSTTTKK48 pKa = 10.56DD49 pKa = 3.47GPLNSIADD57 pKa = 4.0GEE59 pKa = 4.65STAEE63 pKa = 4.04FTSIAYY69 pKa = 10.06RR70 pKa = 11.84EE71 pKa = 4.62DD72 pKa = 3.77GSDD75 pKa = 5.22VIALWKK81 pKa = 10.37QMRR84 pKa = 11.84KK85 pKa = 6.9WFRR88 pKa = 11.84DD89 pKa = 3.32NEE91 pKa = 4.29LVEE94 pKa = 4.71FWQVDD99 pKa = 3.9FGSRR103 pKa = 11.84RR104 pKa = 11.84TEE106 pKa = 3.96DD107 pKa = 3.11GKK109 pKa = 11.14EE110 pKa = 4.03VFDD113 pKa = 3.48VDD115 pKa = 3.86YY116 pKa = 10.72YY117 pKa = 10.77QGKK120 pKa = 7.74FTSFEE125 pKa = 3.99LSAPADD131 pKa = 3.82DD132 pKa = 4.8LVEE135 pKa = 4.21LSFEE139 pKa = 4.07FAIDD143 pKa = 3.65GKK145 pKa = 10.8GVEE148 pKa = 4.62DD149 pKa = 3.78HH150 pKa = 7.19TDD152 pKa = 3.09TLTAEE157 pKa = 4.16QLQAIQSGMYY167 pKa = 10.11DD168 pKa = 4.06YY169 pKa = 7.83EE170 pKa = 4.25TLAATGGATPTTPLTNTLHH189 pKa = 5.88TPVAKK194 pKa = 10.39DD195 pKa = 3.11VNTTVDD201 pKa = 3.55VVPNAKK207 pKa = 10.1DD208 pKa = 3.57AIEE211 pKa = 4.32NSATLPADD219 pKa = 3.64AVYY222 pKa = 10.63SWVTEE227 pKa = 4.6PIVSAPATVQADD239 pKa = 3.84VKK241 pKa = 9.93VTYY244 pKa = 10.86ADD246 pKa = 3.67ASTDD250 pKa = 3.4TVTVNVIVAA259 pKa = 4.18

Molecular weight: 28.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B2K3|A0A4D6B2K3_9CAUD AAA_23 domain-containing protein OS=Streptococcus phage Javan406 OX=2548152 GN=Javan406_0010 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.05RR3 pKa = 11.84KK4 pKa = 9.78KK5 pKa = 9.7IHH7 pKa = 6.38LLSFFQLMICGNTDD21 pKa = 2.71TRR23 pKa = 11.84LKK25 pKa = 9.28AQRR28 pKa = 11.84VAWQSLKK35 pKa = 10.32IVPLRR40 pKa = 11.84LAA42 pKa = 4.04

Molecular weight: 4.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11436

42

1119

215.8

24.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.695 ± 0.91

0.533 ± 0.07

6.523 ± 0.405

6.689 ± 0.585

4.232 ± 0.261

5.859 ± 0.604

1.6 ± 0.135

7.188 ± 0.288

8.421 ± 0.505

8.202 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.233

5.701 ± 0.389

2.956 ± 0.304

4.232 ± 0.301

4.066 ± 0.316

6.235 ± 0.433

6.173 ± 0.289

6.051 ± 0.24

1.128 ± 0.135

3.944 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski