Ferrovibrio terrae
Average proteome isoelectric point is 6.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4107 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516H517|A0A516H517_9PROT Protein TonB OS=Ferrovibrio terrae OX=2594003 GN=FNB15_16760 PE=3 SV=1
MM1 pKa = 7.57 SPATDD6 pKa = 3.43 SDD8 pKa = 3.93 SGGEE12 pKa = 3.97 KK13 pKa = 9.5 MAVITGDD20 pKa = 3.1 ATDD23 pKa = 3.57 NVLNGTAAADD33 pKa = 3.5 QIYY36 pKa = 10.08 GLNGNDD42 pKa = 3.57 TLTGGNGIDD51 pKa = 4.76 LLDD54 pKa = 4.84 GGDD57 pKa = 4.61 GDD59 pKa = 4.71 DD60 pKa = 4.47 SLYY63 pKa = 11.29 GGNQNDD69 pKa = 4.21 TLNGGNGIDD78 pKa = 3.2 TAVFEE83 pKa = 4.66 KK84 pKa = 10.72 PIGRR88 pKa = 11.84 YY89 pKa = 9.55 RR90 pKa = 11.84 ITYY93 pKa = 8.01 NNGVITVDD101 pKa = 3.27 AQSGYY106 pKa = 11.03 EE107 pKa = 4.44 DD108 pKa = 4.2 VDD110 pKa = 3.74 TLTGMEE116 pKa = 4.01 KK117 pKa = 10.66 LKK119 pKa = 11.13 FNGLVYY125 pKa = 10.76 DD126 pKa = 3.92 FTGWTGGPLPEE137 pKa = 4.23 PTMPAGTVYY146 pKa = 9.25 GTEE149 pKa = 3.85 GHH151 pKa = 5.77 EE152 pKa = 4.35 TLTGTGGVDD161 pKa = 3.4 YY162 pKa = 11.13 LHH164 pKa = 7.05 GLGGDD169 pKa = 3.58 DD170 pKa = 4.66 VMIGGAGGDD179 pKa = 3.49 ILVGGSGNDD188 pKa = 3.3 TASYY192 pKa = 9.85 AGSSAGVTVSLEE204 pKa = 4.2 TGLGFYY210 pKa = 10.91 GDD212 pKa = 3.79 AANDD216 pKa = 3.51 VLHH219 pKa = 6.77 GFEE222 pKa = 4.44 NLQGSSFNDD231 pKa = 3.33 TLTGNGGDD239 pKa = 4.12 NIFISSGGVDD249 pKa = 5.26 DD250 pKa = 3.82 ITGGAGVDD258 pKa = 3.05 WVDD261 pKa = 3.34 YY262 pKa = 11.3 SDD264 pKa = 3.36 IVKK267 pKa = 9.37 PAGMTGMFFQRR278 pKa = 11.84 GTPTNIASYY287 pKa = 9.81 FYY289 pKa = 10.27 NASINPFKK297 pKa = 10.53 DD298 pKa = 2.77 TLRR301 pKa = 11.84 QVEE304 pKa = 4.42 GVRR307 pKa = 11.84 GSAYY311 pKa = 10.55 DD312 pKa = 3.67 DD313 pKa = 3.66 VYY315 pKa = 11.5 MNILTSDD322 pKa = 3.57 DD323 pKa = 3.83 MFEE326 pKa = 4.74 GGAGADD332 pKa = 3.19 IFDD335 pKa = 3.97 GRR337 pKa = 11.84 GGNDD341 pKa = 3.08 TLVYY345 pKa = 8.12 THH347 pKa = 6.82 SAAGVNVNLATGAASGGDD365 pKa = 3.28 ATGDD369 pKa = 3.53 QFTGSYY375 pKa = 7.2 VWRR378 pKa = 11.84 GTTRR382 pKa = 11.84 NLAGATNLSGSEE394 pKa = 4.12 HH395 pKa = 7.42 ADD397 pKa = 3.24 TLTGSSVANVLRR409 pKa = 11.84 GADD412 pKa = 3.48 GDD414 pKa = 3.89 DD415 pKa = 3.53 TLRR418 pKa = 11.84 GDD420 pKa = 4.51 GGNDD424 pKa = 3.39 TLDD427 pKa = 3.52 GGAGVDD433 pKa = 3.14 IAVYY437 pKa = 10.46 ALSLNNYY444 pKa = 6.86 TISYY448 pKa = 9.23 NSGAITVAANIGTEE462 pKa = 4.28 GTDD465 pKa = 3.0 TLTGIEE471 pKa = 4.63 KK472 pKa = 10.82 LLFANGTLDD481 pKa = 3.45 LTEE484 pKa = 4.17 WTGGPLPPLLGPNDD498 pKa = 3.4 IMGSSGDD505 pKa = 3.65 NVLTGTAAGQSIYY518 pKa = 11.23 GMAGNDD524 pKa = 3.99 SISGAGGNDD533 pKa = 3.22 VLTGGDD539 pKa = 3.52 GSDD542 pKa = 3.35 TYY544 pKa = 11.54 LVARR548 pKa = 11.84 GDD550 pKa = 3.91 GVDD553 pKa = 5.37 DD554 pKa = 3.96 IVQAGITDD562 pKa = 3.74 AAGTTDD568 pKa = 3.5 AVLFGSGVSYY578 pKa = 10.67 DD579 pKa = 3.77 QLWFRR584 pKa = 11.84 QVGNDD589 pKa = 3.93 LRR591 pKa = 11.84 IDD593 pKa = 4.04 VIGEE597 pKa = 4.15 SASSVLLKK605 pKa = 10.3 SWYY608 pKa = 8.16 TDD610 pKa = 2.8 STRR613 pKa = 11.84 RR614 pKa = 11.84 VDD616 pKa = 5.15 SIQTTDD622 pKa = 3.26 GSHH625 pKa = 6.25 SLSAANVQALVDD637 pKa = 3.91 AMATYY642 pKa = 7.6 TQPGTGDD649 pKa = 3.5 FTLSPQLASDD659 pKa = 4.6 LAPVFATTWAA669 pKa = 3.56
Molecular weight: 67.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.579
IPC_protein 3.656
Toseland 3.401
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.643
Rodwell 3.465
Grimsley 3.3
Solomon 3.668
Lehninger 3.617
Nozaki 3.77
DTASelect 4.101
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.783
Patrickios 1.418
IPC_peptide 3.656
IPC2_peptide 3.745
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A516H194|A0A516H194_9PROT 3-alpha 7-alpha 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase OS=Ferrovibrio terrae OX=2594003 GN=FNB15_09755 PE=4 SV=1
MM1 pKa = 7.74 SKK3 pKa = 8.95 RR4 pKa = 11.84 TYY6 pKa = 10.27 QPSKK10 pKa = 9.73 LVRR13 pKa = 11.84 KK14 pKa = 9.15 RR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.42 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATVGGRR29 pKa = 11.84 NVIASRR35 pKa = 11.84 RR36 pKa = 11.84 AQGRR40 pKa = 11.84 KK41 pKa = 9.31 KK42 pKa = 10.65 LSAA45 pKa = 3.91
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4107
0
4107
1303169
29
3226
317.3
34.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.612 ± 0.055
0.84 ± 0.011
5.528 ± 0.028
5.232 ± 0.034
3.649 ± 0.024
8.481 ± 0.039
2.044 ± 0.021
5.168 ± 0.028
3.698 ± 0.035
10.576 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.628 ± 0.02
2.697 ± 0.02
5.192 ± 0.038
3.614 ± 0.032
6.844 ± 0.038
5.073 ± 0.027
5.284 ± 0.054
7.229 ± 0.032
1.339 ± 0.015
2.271 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here