Amino acid dipepetide frequency for Alcaligenes sp. EGD-AK7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.422AlaAla: 12.422 ± 0.144
1.279AlaCys: 1.279 ± 0.031
5.581AlaAsp: 5.581 ± 0.082
5.436AlaGlu: 5.436 ± 0.064
3.746AlaPhe: 3.746 ± 0.071
8.604AlaGly: 8.604 ± 0.129
2.237AlaHis: 2.237 ± 0.045
5.387AlaIle: 5.387 ± 0.065
3.399AlaLys: 3.399 ± 0.063
13.325AlaLeu: 13.325 ± 0.125
3.211AlaMet: 3.211 ± 0.054
2.876AlaAsn: 2.876 ± 0.054
4.617AlaPro: 4.617 ± 0.073
6.464AlaGln: 6.464 ± 0.092
6.915AlaArg: 6.915 ± 0.085
6.517AlaSer: 6.517 ± 0.083
4.938AlaThr: 4.938 ± 0.064
7.561AlaVal: 7.561 ± 0.08
1.74AlaTrp: 1.74 ± 0.043
2.588AlaTyr: 2.588 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.997CysAla: 0.997 ± 0.027
0.129CysCys: 0.129 ± 0.009
0.47CysAsp: 0.47 ± 0.017
0.461CysGlu: 0.461 ± 0.02
0.316CysPhe: 0.316 ± 0.017
0.921CysGly: 0.921 ± 0.026
0.257CysHis: 0.257 ± 0.015
0.426CysIle: 0.426 ± 0.016
0.253CysLys: 0.253 ± 0.013
1.019CysLeu: 1.019 ± 0.035
0.238CysMet: 0.238 ± 0.012
0.249CysAsn: 0.249 ± 0.013
0.5CysPro: 0.5 ± 0.022
0.382CysGln: 0.382 ± 0.019
0.541CysArg: 0.541 ± 0.024
0.555CysSer: 0.555 ± 0.02
0.499CysThr: 0.499 ± 0.019
0.72CysVal: 0.72 ± 0.028
0.143CysTrp: 0.143 ± 0.009
0.239CysTyr: 0.239 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
5.334AspAla: 5.334 ± 0.062
0.49AspCys: 0.49 ± 0.02
2.474AspAsp: 2.474 ± 0.054
3.127AspGlu: 3.127 ± 0.061
2.032AspPhe: 2.032 ± 0.045
3.975AspGly: 3.975 ± 0.063
1.104AspHis: 1.104 ± 0.037
2.71AspIle: 2.71 ± 0.046
1.92AspLys: 1.92 ± 0.043
5.762AspLeu: 5.762 ± 0.075
1.357AspMet: 1.357 ± 0.032
1.385AspAsn: 1.385 ± 0.034
2.849AspPro: 2.849 ± 0.048
2.495AspGln: 2.495 ± 0.05
3.014AspArg: 3.014 ± 0.052
2.872AspSer: 2.872 ± 0.05
2.63AspThr: 2.63 ± 0.047
3.723AspVal: 3.723 ± 0.069
1.045AspTrp: 1.045 ± 0.027
1.628AspTyr: 1.628 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
5.581GluAla: 5.581 ± 0.082
0.396GluCys: 0.396 ± 0.017
2.358GluAsp: 2.358 ± 0.045
2.731GluGlu: 2.731 ± 0.056
1.852GluPhe: 1.852 ± 0.044
3.654GluGly: 3.654 ± 0.059
1.609GluHis: 1.609 ± 0.039
2.738GluIle: 2.738 ± 0.05
2.07GluLys: 2.07 ± 0.054
6.432GluLeu: 6.432 ± 0.073
1.323GluMet: 1.323 ± 0.032
1.644GluAsn: 1.644 ± 0.04
2.592GluPro: 2.592 ± 0.047
4.31GluGln: 4.31 ± 0.076
4.376GluArg: 4.376 ± 0.076
2.891GluSer: 2.891 ± 0.044
2.593GluThr: 2.593 ± 0.042
3.616GluVal: 3.616 ± 0.063
0.724GluTrp: 0.724 ± 0.023
1.244GluTyr: 1.244 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
3.612PheAla: 3.612 ± 0.056
0.396PheCys: 0.396 ± 0.019
2.252PheAsp: 2.252 ± 0.043
1.994PheGlu: 1.994 ± 0.04
1.477PhePhe: 1.477 ± 0.037
3.159PheGly: 3.159 ± 0.047
0.751PheHis: 0.751 ± 0.026
1.777PheIle: 1.777 ± 0.04
1.285PheLys: 1.285 ± 0.033
3.579PheLeu: 3.579 ± 0.062
0.939PheMet: 0.939 ± 0.029
1.283PheAsn: 1.283 ± 0.035
1.515PhePro: 1.515 ± 0.035
1.306PheGln: 1.306 ± 0.032
1.753PheArg: 1.753 ± 0.041
2.582PheSer: 2.582 ± 0.053
1.943PheThr: 1.943 ± 0.042
2.676PheVal: 2.676 ± 0.053
0.583PheTrp: 0.583 ± 0.024
1.025PheTyr: 1.025 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
7.454GlyAla: 7.454 ± 0.091
0.759GlyCys: 0.759 ± 0.027
3.561GlyAsp: 3.561 ± 0.063
3.876GlyGlu: 3.876 ± 0.065
3.139GlyPhe: 3.139 ± 0.051
5.93GlyGly: 5.93 ± 0.127
1.772GlyHis: 1.772 ± 0.037
4.177GlyIle: 4.177 ± 0.066
3.252GlyLys: 3.252 ± 0.058
9.395GlyLeu: 9.395 ± 0.099
2.326GlyMet: 2.326 ± 0.038
2.178GlyAsn: 2.178 ± 0.055
2.894GlyPro: 2.894 ± 0.05
4.175GlyGln: 4.175 ± 0.07
4.759GlyArg: 4.759 ± 0.069
4.665GlySer: 4.665 ± 0.075
4.049GlyThr: 4.049 ± 0.104
6.053GlyVal: 6.053 ± 0.067
1.371GlyTrp: 1.371 ± 0.039
2.27GlyTyr: 2.27 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
2.378HisAla: 2.378 ± 0.052
0.26HisCys: 0.26 ± 0.016
1.186HisAsp: 1.186 ± 0.035
1.151HisGlu: 1.151 ± 0.033
0.896HisPhe: 0.896 ± 0.026
1.747HisGly: 1.747 ± 0.042
0.661HisHis: 0.661 ± 0.029
1.203HisIle: 1.203 ± 0.032
0.665HisLys: 0.665 ± 0.022
2.322HisLeu: 2.322 ± 0.046
0.506HisMet: 0.506 ± 0.021
0.597HisAsn: 0.597 ± 0.024
1.428HisPro: 1.428 ± 0.034
1.024HisGln: 1.024 ± 0.032
1.307HisArg: 1.307 ± 0.034
1.264HisSer: 1.264 ± 0.036
1.112HisThr: 1.112 ± 0.029
1.46HisVal: 1.46 ± 0.031
0.431HisTrp: 0.431 ± 0.022
0.696HisTyr: 0.696 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.8IleAla: 5.8 ± 0.08
0.484IleCys: 0.484 ± 0.022
2.97IleAsp: 2.97 ± 0.05
3.312IleGlu: 3.312 ± 0.05
1.571IlePhe: 1.571 ± 0.038
4.173IleGly: 4.173 ± 0.071
1.057IleHis: 1.057 ± 0.031
2.212IleIle: 2.212 ± 0.047
1.75IleLys: 1.75 ± 0.041
4.626IleLeu: 4.626 ± 0.06
1.112IleMet: 1.112 ± 0.035
1.747IleAsn: 1.747 ± 0.046
2.407IlePro: 2.407 ± 0.045
1.995IleGln: 1.995 ± 0.049
2.871IleArg: 2.871 ± 0.047
3.286IleSer: 3.286 ± 0.055
2.574IleThr: 2.574 ± 0.052
3.763IleVal: 3.763 ± 0.056
0.649IleTrp: 0.649 ± 0.027
1.248IleTyr: 1.248 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
3.9LysAla: 3.9 ± 0.069
0.132LysCys: 0.132 ± 0.012
1.843LysAsp: 1.843 ± 0.039
1.96LysGlu: 1.96 ± 0.044
0.802LysPhe: 0.802 ± 0.025
2.597LysGly: 2.597 ± 0.052
0.701LysHis: 0.701 ± 0.024
1.54LysIle: 1.54 ± 0.044
1.362LysLys: 1.362 ± 0.044
3.438LysLeu: 3.438 ± 0.054
0.812LysMet: 0.812 ± 0.024
1.074LysAsn: 1.074 ± 0.031
1.945LysPro: 1.945 ± 0.04
1.768LysGln: 1.768 ± 0.041
2.146LysArg: 2.146 ± 0.047
1.81LysSer: 1.81 ± 0.042
1.887LysThr: 1.887 ± 0.041
2.485LysVal: 2.485 ± 0.053
0.398LysTrp: 0.398 ± 0.019
0.708LysTyr: 0.708 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
13.239LeuAla: 13.239 ± 0.121
1.096LeuCys: 1.096 ± 0.029
6.362LeuAsp: 6.362 ± 0.079
6.138LeuGlu: 6.138 ± 0.092
3.97LeuPhe: 3.97 ± 0.07
8.915LeuGly: 8.915 ± 0.096
2.393LeuHis: 2.393 ± 0.045
5.826LeuIle: 5.826 ± 0.088
3.623LeuLys: 3.623 ± 0.062
12.026LeuLeu: 12.026 ± 0.145
2.892LeuMet: 2.892 ± 0.051
3.661LeuAsn: 3.661 ± 0.058
6.059LeuPro: 6.059 ± 0.077
4.757LeuGln: 4.757 ± 0.078
7.366LeuArg: 7.366 ± 0.094
7.993LeuSer: 7.993 ± 0.092
5.464LeuThr: 5.464 ± 0.07
7.397LeuVal: 7.397 ± 0.093
1.279LeuTrp: 1.279 ± 0.038
2.416LeuTyr: 2.416 ± 0.042
0.0LeuXaa: 0.0 ± 0.0
Met
3.024MetAla: 3.024 ± 0.048
0.173MetCys: 0.173 ± 0.012
1.261MetAsp: 1.261 ± 0.032
1.229MetGlu: 1.229 ± 0.026
0.743MetPhe: 0.743 ± 0.027
2.082MetGly: 2.082 ± 0.037
0.484MetHis: 0.484 ± 0.018
1.169MetIle: 1.169 ± 0.032
0.975MetLys: 0.975 ± 0.031
2.861MetLeu: 2.861 ± 0.047
0.755MetMet: 0.755 ± 0.027
0.959MetAsn: 0.959 ± 0.031
1.525MetPro: 1.525 ± 0.031
1.288MetGln: 1.288 ± 0.03
1.615MetArg: 1.615 ± 0.033
1.972MetSer: 1.972 ± 0.039
1.527MetThr: 1.527 ± 0.033
1.82MetVal: 1.82 ± 0.042
0.245MetTrp: 0.245 ± 0.013
0.446MetTyr: 0.446 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.08AsnAla: 3.08 ± 0.058
0.245AsnCys: 0.245 ± 0.014
1.37AsnAsp: 1.37 ± 0.035
1.546AsnGlu: 1.546 ± 0.036
1.04AsnPhe: 1.04 ± 0.031
2.345AsnGly: 2.345 ± 0.058
0.623AsnHis: 0.623 ± 0.02
1.565AsnIle: 1.565 ± 0.038
1.114AsnLys: 1.114 ± 0.032
3.125AsnLeu: 3.125 ± 0.065
0.751AsnMet: 0.751 ± 0.027
1.021AsnAsn: 1.021 ± 0.038
2.001AsnPro: 2.001 ± 0.047
1.302AsnGln: 1.302 ± 0.035
1.779AsnArg: 1.779 ± 0.037
1.749AsnSer: 1.749 ± 0.04
1.77AsnThr: 1.77 ± 0.048
2.032AsnVal: 2.032 ± 0.045
0.498AsnTrp: 0.498 ± 0.022
0.85AsnTyr: 0.85 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
5.77ProAla: 5.77 ± 0.082
0.366ProCys: 0.366 ± 0.016
3.154ProAsp: 3.154 ± 0.051
3.703ProGlu: 3.703 ± 0.067
1.783ProPhe: 1.783 ± 0.037
3.874ProGly: 3.874 ± 0.063
1.013ProHis: 1.013 ± 0.031
2.16ProIle: 2.16 ± 0.044
1.529ProLys: 1.529 ± 0.035
4.985ProLeu: 4.985 ± 0.075
1.245ProMet: 1.245 ± 0.026
1.414ProAsn: 1.414 ± 0.032
1.969ProPro: 1.969 ± 0.048
2.145ProGln: 2.145 ± 0.045
2.385ProArg: 2.385 ± 0.045
3.033ProSer: 3.033 ± 0.061
2.339ProThr: 2.339 ± 0.046
4.209ProVal: 4.209 ± 0.058
0.821ProTrp: 0.821 ± 0.029
1.268ProTyr: 1.268 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
7.513GlnAla: 7.513 ± 0.12
0.363GlnCys: 0.363 ± 0.018
2.629GlnAsp: 2.629 ± 0.053
2.954GlnGlu: 2.954 ± 0.061
1.539GlnPhe: 1.539 ± 0.038
4.166GlnGly: 4.166 ± 0.053
1.17GlnHis: 1.17 ± 0.033
2.447GlnIle: 2.447 ± 0.042
1.408GlnLys: 1.408 ± 0.032
4.777GlnLeu: 4.777 ± 0.082
1.122GlnMet: 1.122 ± 0.03
1.337GlnAsn: 1.337 ± 0.036
2.174GlnPro: 2.174 ± 0.045
2.767GlnGln: 2.767 ± 0.067
3.418GlnArg: 3.418 ± 0.065
2.839GlnSer: 2.839 ± 0.057
2.585GlnThr: 2.585 ± 0.053
3.531GlnVal: 3.531 ± 0.063
0.888GlnTrp: 0.888 ± 0.027
1.069GlnTyr: 1.069 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
5.875ArgAla: 5.875 ± 0.077
0.596ArgCys: 0.596 ± 0.023
3.188ArgAsp: 3.188 ± 0.057
3.708ArgGlu: 3.708 ± 0.059
2.556ArgPhe: 2.556 ± 0.049
3.737ArgGly: 3.737 ± 0.053
1.654ArgHis: 1.654 ± 0.037
3.506ArgIle: 3.506 ± 0.055
2.093ArgLys: 2.093 ± 0.039
7.554ArgLeu: 7.554 ± 0.094
1.686ArgMet: 1.686 ± 0.041
1.969ArgAsn: 1.969 ± 0.046
2.838ArgPro: 2.838 ± 0.055
3.574ArgGln: 3.574 ± 0.056
4.385ArgArg: 4.385 ± 0.074
3.834ArgSer: 3.834 ± 0.058
2.668ArgThr: 2.668 ± 0.049
4.326ArgVal: 4.326 ± 0.065
1.036ArgTrp: 1.036 ± 0.033
1.989ArgTyr: 1.989 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.208SerAla: 6.208 ± 0.069
0.535SerCys: 0.535 ± 0.018
2.95SerAsp: 2.95 ± 0.049
2.994SerGlu: 2.994 ± 0.047
2.386SerPhe: 2.386 ± 0.053
5.415SerGly: 5.415 ± 0.077
1.333SerHis: 1.333 ± 0.036
2.99SerIle: 2.99 ± 0.065
1.912SerLys: 1.912 ± 0.041
7.396SerLeu: 7.396 ± 0.083
1.704SerMet: 1.704 ± 0.038
1.899SerAsn: 1.899 ± 0.046
3.017SerPro: 3.017 ± 0.051
2.848SerGln: 2.848 ± 0.052
3.738SerArg: 3.738 ± 0.062
4.082SerSer: 4.082 ± 0.079
3.249SerThr: 3.249 ± 0.057
4.433SerVal: 4.433 ± 0.063
1.009SerTrp: 1.009 ± 0.03
1.637SerTyr: 1.637 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.11ThrAla: 5.11 ± 0.065
0.418ThrCys: 0.418 ± 0.018
2.495ThrAsp: 2.495 ± 0.05
2.481ThrGlu: 2.481 ± 0.043
1.598ThrPhe: 1.598 ± 0.04
4.399ThrGly: 4.399 ± 0.07
1.126ThrHis: 1.126 ± 0.029
2.456ThrIle: 2.456 ± 0.042
1.178ThrLys: 1.178 ± 0.036
6.447ThrLeu: 6.447 ± 0.089
1.17ThrMet: 1.17 ± 0.037
1.212ThrAsn: 1.212 ± 0.034
3.155ThrPro: 3.155 ± 0.048
2.489ThrGln: 2.489 ± 0.045
3.03ThrArg: 3.03 ± 0.056
2.766ThrSer: 2.766 ± 0.053
2.477ThrThr: 2.477 ± 0.051
4.181ThrVal: 4.181 ± 0.064
0.665ThrTrp: 0.665 ± 0.023
1.123ThrTyr: 1.123 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.703ValAla: 7.703 ± 0.089
0.786ValCys: 0.786 ± 0.025
3.77ValAsp: 3.77 ± 0.06
3.868ValGlu: 3.868 ± 0.055
2.821ValPhe: 2.821 ± 0.055
5.189ValGly: 5.189 ± 0.072
1.466ValHis: 1.466 ± 0.033
3.468ValIle: 3.468 ± 0.059
2.244ValLys: 2.244 ± 0.05
8.849ValLeu: 8.849 ± 0.098
1.996ValMet: 1.996 ± 0.04
2.148ValAsn: 2.148 ± 0.046
3.684ValPro: 3.684 ± 0.051
3.483ValGln: 3.483 ± 0.058
4.416ValArg: 4.416 ± 0.072
4.619ValSer: 4.619 ± 0.063
3.607ValThr: 3.607 ± 0.057
5.561ValVal: 5.561 ± 0.076
0.992ValTrp: 0.992 ± 0.027
1.726ValTyr: 1.726 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
1.337TrpAla: 1.337 ± 0.037
0.156TrpCys: 0.156 ± 0.011
0.647TrpAsp: 0.647 ± 0.024
0.637TrpGlu: 0.637 ± 0.02
0.597TrpPhe: 0.597 ± 0.023
1.002TrpGly: 1.002 ± 0.03
0.388TrpHis: 0.388 ± 0.018
0.763TrpIle: 0.763 ± 0.03
0.487TrpLys: 0.487 ± 0.02
2.179TrpLeu: 2.179 ± 0.045
0.441TrpMet: 0.441 ± 0.02
0.5TrpAsn: 0.5 ± 0.021
0.721TrpPro: 0.721 ± 0.024
0.851TrpGln: 0.851 ± 0.027
1.178TrpArg: 1.178 ± 0.035
0.872TrpSer: 0.872 ± 0.029
0.739TrpThr: 0.739 ± 0.028
1.096TrpVal: 1.096 ± 0.029
0.272TrpTrp: 0.272 ± 0.013
0.355TrpTyr: 0.355 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.05
0.258TyrCys: 0.258 ± 0.014
1.406TyrAsp: 1.406 ± 0.037
1.428TyrGlu: 1.428 ± 0.034
1.022TyrPhe: 1.022 ± 0.028
2.17TyrGly: 2.17 ± 0.051
0.493TyrHis: 0.493 ± 0.022
1.089TyrIle: 1.089 ± 0.027
0.79TyrLys: 0.79 ± 0.026
2.774TyrLeu: 2.774 ± 0.046
0.565TyrMet: 0.565 ± 0.021
0.652TyrAsn: 0.652 ± 0.023
1.276TyrPro: 1.276 ± 0.03
1.271TyrGln: 1.271 ± 0.033
1.782TyrArg: 1.782 ± 0.04
1.536TyrSer: 1.536 ± 0.042
1.328TyrThr: 1.328 ± 0.034
1.75TyrVal: 1.75 ± 0.036
0.427TyrTrp: 0.427 ± 0.021
0.714TyrTyr: 0.714 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3984 proteins (1255071 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski