Brown greater galago prosimian foamy virus
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A088FA16|A0A088FA16_9RETR Bel1 protein OS=Brown greater galago prosimian foamy virus OX=2170139 GN=bel1 PE=4 SV=1
MM1 pKa = 7.52 ASWNPGQKK9 pKa = 10.27 GINIPQNDD17 pKa = 4.32 EE18 pKa = 3.87 SDD20 pKa = 3.86 LPQPRR25 pKa = 11.84 PLPALPASEE34 pKa = 5.13 FMDD37 pKa = 3.58 HH38 pKa = 6.77 NYY40 pKa = 10.52 VGMRR44 pKa = 11.84 QWYY47 pKa = 8.32 NDD49 pKa = 3.56 PSGGYY54 pKa = 8.49 PDD56 pKa = 4.9 LGNEE60 pKa = 4.12 EE61 pKa = 4.16 LHH63 pKa = 7.43 DD64 pKa = 3.98 VLFSLAYY71 pKa = 10.16 NCLHH75 pKa = 7.16 FYY77 pKa = 10.7 KK78 pKa = 10.26 SLPIVRR84 pKa = 11.84 QRR86 pKa = 11.84 FKK88 pKa = 11.1 CYY90 pKa = 10.32 LEE92 pKa = 4.56 PSFQRR97 pKa = 11.84 VGIYY101 pKa = 9.58 NICFQCKK108 pKa = 6.91 QCYY111 pKa = 8.9 KK112 pKa = 10.83 VIIEE116 pKa = 4.45 SKK118 pKa = 8.72 PVKK121 pKa = 10.15 YY122 pKa = 10.52 DD123 pKa = 3.4 PEE125 pKa = 4.51 VKK127 pKa = 10.39 FFTLLLSNFQKK138 pKa = 10.87 SSWRR142 pKa = 11.84 RR143 pKa = 11.84 SRR145 pKa = 11.84 MCRR148 pKa = 11.84 HH149 pKa = 6.36 LEE151 pKa = 3.6 WHH153 pKa = 6.47 EE154 pKa = 4.01 NEE156 pKa = 3.99 QFSYY160 pKa = 10.86 RR161 pKa = 11.84 STRR164 pKa = 11.84 PRR166 pKa = 11.84 EE167 pKa = 4.12 QGSEE171 pKa = 4.06 PSPAATSSCSRR182 pKa = 11.84 MDD184 pKa = 3.71 SVPDD188 pKa = 3.76 PPRR191 pKa = 11.84 GPRR194 pKa = 11.84 EE195 pKa = 4.06 CLQGPSMSDD204 pKa = 3.3 CASPIPGPCTSQEE217 pKa = 3.84 PYY219 pKa = 11.19 ADD221 pKa = 2.66 VDD223 pKa = 3.73 AFLEE227 pKa = 4.46 GLLQQPQKK235 pKa = 11.0 GRR237 pKa = 11.84 ADD239 pKa = 3.21 SGMGLDD245 pKa = 5.1 PGAGWTEE252 pKa = 3.74 DD253 pKa = 3.49 LEE255 pKa = 4.86 RR256 pKa = 11.84 FILGDD261 pKa = 3.96 DD262 pKa = 3.81 NSCARR267 pKa = 11.84 DD268 pKa = 3.42 WTDD271 pKa = 4.09 SMHH274 pKa = 7.16 SDD276 pKa = 3.18 SS277 pKa = 5.1
Molecular weight: 31.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.98
IPC2_protein 5.118
IPC_protein 5.067
Toseland 5.016
ProMoST 5.232
Dawson 5.08
Bjellqvist 5.207
Wikipedia 4.978
Rodwell 4.991
Grimsley 4.952
Solomon 5.08
Lehninger 5.041
Nozaki 5.207
DTASelect 5.385
Thurlkill 5.041
EMBOSS 5.029
Sillero 5.283
Patrickios 3.554
IPC_peptide 5.092
IPC2_peptide 5.27
IPC2.peptide.svr19 5.242
Protein with the highest isoelectric point:
>tr|A0A088FA16|A0A088FA16_9RETR Bel1 protein OS=Brown greater galago prosimian foamy virus OX=2170139 GN=bel1 PE=4 SV=1
MM1 pKa = 7.12 SQPSASGSAGAGGAPQQPPPPPPQPGPAAPVPRR34 pKa = 11.84 AQIGYY39 pKa = 10.23 GDD41 pKa = 5.11 LDD43 pKa = 3.66 VLLLQQEE50 pKa = 4.39 YY51 pKa = 11.06 HH52 pKa = 7.03 LIDD55 pKa = 4.68 PNLQVQHH62 pKa = 7.28 LDD64 pKa = 3.29 TLLVRR69 pKa = 11.84 ITGGNWGPGDD79 pKa = 3.39 RR80 pKa = 11.84 FARR83 pKa = 11.84 IEE85 pKa = 3.99 VLLRR89 pKa = 11.84 DD90 pKa = 3.85 TLGPLQQPRR99 pKa = 11.84 YY100 pKa = 9.27 RR101 pKa = 11.84 YY102 pKa = 10.11 AAMQQADD109 pKa = 4.2 LRR111 pKa = 11.84 NDD113 pKa = 3.73 IILHH117 pKa = 6.43 LNYY120 pKa = 9.76 QDD122 pKa = 4.81 AIIIFDD128 pKa = 3.99 MIIPSEE134 pKa = 4.19 GVHH137 pKa = 5.14 RR138 pKa = 11.84 HH139 pKa = 4.91 GPMFDD144 pKa = 3.68 GLWIHH149 pKa = 7.07 GDD151 pKa = 3.62 DD152 pKa = 3.83 YY153 pKa = 12.04 SMNFQPITAHH163 pKa = 6.32 EE164 pKa = 4.99 LYY166 pKa = 10.82 LLPQQVLTEE175 pKa = 4.08 EE176 pKa = 4.54 VEE178 pKa = 4.71 LLTEE182 pKa = 4.1 VCNRR186 pKa = 11.84 MADD189 pKa = 3.75 WIRR192 pKa = 11.84 RR193 pKa = 11.84 HH194 pKa = 5.65 RR195 pKa = 11.84 CGGGSGSSQPPPPPPPAVPVLPSAPPASSLPLPPQGWGISPPVATSTPGAAGHH248 pKa = 6.27 SSSAGPNISLGGTYY262 pKa = 9.93 VPPPVAPPAPVIGGPGGPGQLPAMVQVLPAQPVVIPINVIRR303 pKa = 11.84 SVCGDD308 pKa = 3.68 TPSNPQDD315 pKa = 3.03 IPLWMGRR322 pKa = 11.84 IIPAIEE328 pKa = 4.16 GVFPIDD334 pKa = 3.81 NPNLRR339 pKa = 11.84 MRR341 pKa = 11.84 VVNALLALHH350 pKa = 7.04 PGLAITEE357 pKa = 4.57 LNAQTWGQVLAVLHH371 pKa = 5.69 MRR373 pKa = 11.84 ALGHH377 pKa = 5.99 TALHH381 pKa = 5.63 QLPALLEE388 pKa = 4.56 TIVKK392 pKa = 9.24 TDD394 pKa = 4.56 GILPAYY400 pKa = 9.49 NMGMEE405 pKa = 4.49 VTQQDD410 pKa = 3.94 FSYY413 pKa = 10.79 VWGILRR419 pKa = 11.84 TLLPGQAFVLSMQNEE434 pKa = 4.76 LDD436 pKa = 3.81 RR437 pKa = 11.84 LPAAQRR443 pKa = 11.84 PGMFPGLLQRR453 pKa = 11.84 TLDD456 pKa = 3.58 ILGLNSRR463 pKa = 11.84 GQNIQKK469 pKa = 9.26 TNTQQQAPKK478 pKa = 9.87 RR479 pKa = 11.84 GQKK482 pKa = 9.44 PKK484 pKa = 9.98 PRR486 pKa = 11.84 LPPVHH491 pKa = 6.87 RR492 pKa = 11.84 RR493 pKa = 11.84 PAPFTPPATPSPRR506 pKa = 11.84 QQASASPSSQGDD518 pKa = 3.28 NRR520 pKa = 11.84 SPQPQGRR527 pKa = 11.84 GTYY530 pKa = 9.67 GPSRR534 pKa = 11.84 GGGSGPRR541 pKa = 11.84 YY542 pKa = 9.0 NFRR545 pKa = 11.84 PRR547 pKa = 11.84 VQPPDD552 pKa = 3.03 RR553 pKa = 11.84 YY554 pKa = 10.92 GFGRR558 pKa = 11.84 GQGGRR563 pKa = 11.84 SSIGAQDD570 pKa = 3.58 NQQPGQGGQRR580 pKa = 11.84 TQQTNQNRR588 pKa = 11.84 NQGNATGGRR597 pKa = 11.84 TQPQNRR603 pKa = 11.84 TVNTVRR609 pKa = 11.84 VTQTNPQGGSSVSNPAVTTSQNTGTGSATQSSSSS643 pKa = 3.26
Molecular weight: 68.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.879
IPC2_protein 9.209
IPC_protein 9.897
Toseland 9.487
ProMoST 9.545
Dawson 9.867
Bjellqvist 9.721
Wikipedia 10.16
Rodwell 9.867
Grimsley 10.014
Solomon 9.926
Lehninger 9.853
Nozaki 9.604
DTASelect 9.692
Thurlkill 9.677
EMBOSS 9.955
Sillero 9.809
Patrickios 4.927
IPC_peptide 9.911
IPC2_peptide 8.858
IPC2.peptide.svr19 8.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2381
277
999
595.3
66.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.628 ± 0.564
2.1 ± 0.558
4.872 ± 0.507
4.914 ± 0.996
2.898 ± 0.426
6.846 ± 1.475
2.604 ± 0.231
5.25 ± 0.857
3.276 ± 1.006
9.534 ± 0.977
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.52 ± 0.127
4.326 ± 0.308
9.408 ± 2.067
6.216 ± 1.071
5.712 ± 0.465
7.224 ± 0.758
5.922 ± 0.936
5.502 ± 0.44
2.058 ± 0.397
3.192 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here