Herbinix hemicellulosilytica

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Herbinix

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2640 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H5SGV9|A0A0H5SGV9_HERHM Rubrerythrin domain-containing protein OS=Herbinix hemicellulosilytica OX=1564487 GN=HHT355_1503 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84KK3 pKa = 9.58FEE5 pKa = 4.39CTACGYY11 pKa = 10.27IYY13 pKa = 10.36DD14 pKa = 4.79EE15 pKa = 4.71EE16 pKa = 6.36LGDD19 pKa = 4.47PDD21 pKa = 4.98NGIAPGTKK29 pKa = 8.95WEE31 pKa = 4.91DD32 pKa = 3.84LPDD35 pKa = 3.86DD36 pKa = 4.55WVCPLCGVGKK46 pKa = 10.43EE47 pKa = 4.11DD48 pKa = 4.81FEE50 pKa = 4.98EE51 pKa = 4.72ISS53 pKa = 3.29

Molecular weight: 5.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H5SJR3|A0A0H5SJR3_HERHM Histidine kinase OS=Herbinix hemicellulosilytica OX=1564487 GN=HHT355_1801 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.56KK10 pKa = 9.34PRR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.08GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.3GGRR28 pKa = 11.84KK29 pKa = 8.8VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 9.08KK41 pKa = 9.67LTAA44 pKa = 4.2

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2640

0

2640

848450

30

2152

321.4

36.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.046

1.184 ± 0.02

5.872 ± 0.036

7.263 ± 0.053

4.255 ± 0.035

6.382 ± 0.044

1.518 ± 0.019

9.14 ± 0.047

8.02 ± 0.041

9.135 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.02

5.486 ± 0.037

3.229 ± 0.029

2.631 ± 0.022

4.095 ± 0.036

5.767 ± 0.032

5.124 ± 0.036

6.396 ± 0.04

0.797 ± 0.016

4.64 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski