Mycoplasma columborale

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449B7P0|A0A449B7P0_9MOLU Uncharacterized protein OS=Mycoplasma columborale OX=171282 GN=NCTC10179_00795 PE=4 SV=1
MM1 pKa = 7.81LDD3 pKa = 3.28YY4 pKa = 10.62MDD6 pKa = 4.51AFSYY10 pKa = 11.14NEE12 pKa = 3.98SLWYY16 pKa = 9.94IFEE19 pKa = 4.81DD20 pKa = 4.17LQDD23 pKa = 4.08IDD25 pKa = 4.69DD26 pKa = 4.07QQLLKK31 pKa = 10.93YY32 pKa = 10.1IQTNKK37 pKa = 10.39NEE39 pKa = 3.6WEE41 pKa = 4.39AIKK44 pKa = 10.41RR45 pKa = 11.84KK46 pKa = 10.19YY47 pKa = 10.24KK48 pKa = 10.98LEE50 pKa = 4.04LL51 pKa = 4.3

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449B793|A0A449B793_9MOLU 50S ribosomal protein L6 OS=Mycoplasma columborale OX=171282 GN=MCYN0191 PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.8LCGRR29 pKa = 11.84PHH31 pKa = 7.07AVLRR35 pKa = 11.84KK36 pKa = 9.46YY37 pKa = 10.22KK38 pKa = 9.85ICRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.91EE50 pKa = 4.49GKK52 pKa = 10.2IPGIKK57 pKa = 9.11KK58 pKa = 10.56ASWW61 pKa = 2.94

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

753

0

753

291682

32

6126

387.4

44.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.863 ± 0.12

0.403 ± 0.024

5.603 ± 0.125

6.796 ± 0.089

5.202 ± 0.113

4.317 ± 0.096

1.424 ± 0.038

8.182 ± 0.125

9.491 ± 0.085

10.062 ± 0.106

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.588 ± 0.061

7.891 ± 0.16

2.684 ± 0.043

4.006 ± 0.072

2.923 ± 0.053

6.955 ± 0.08

5.501 ± 0.12

5.982 ± 0.076

0.899 ± 0.032

4.227 ± 0.078
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski