Candidatus Marinimicrobia bacterium MT.SAG.4

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Marinimicrobia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8I2C3|A0A4Y8I2C3_9BACT T9SS type A sorting domain-containing protein (Fragment) OS=Candidatus Marinimicrobia bacterium MT.SAG.4 OX=2552981 GN=E3V33_06715 PE=4 SV=1
MM1 pKa = 7.29FKK3 pKa = 10.55KK4 pKa = 10.44IFILSMLSALTIFGCSDD21 pKa = 3.87DD22 pKa = 5.77DD23 pKa = 4.08DD24 pKa = 4.85TNGPTLSNNTLVLSLTGVEE43 pKa = 4.32TLQNGYY49 pKa = 10.0HH50 pKa = 5.28YY51 pKa = 9.74EE52 pKa = 3.7GWAIVGGSPVATGKK66 pKa = 10.78FNVDD70 pKa = 2.97ANGVLEE76 pKa = 4.54DD77 pKa = 3.98LSGNVISGGEE87 pKa = 4.09FVTTTDD93 pKa = 3.77LTDD96 pKa = 3.11ATAIIITIEE105 pKa = 3.99PDD107 pKa = 2.92GDD109 pKa = 3.53TDD111 pKa = 4.62ANPADD116 pKa = 3.63THH118 pKa = 6.17YY119 pKa = 11.17LAGSVSTMSSTLRR132 pKa = 11.84VGHH135 pKa = 7.03ASALGDD141 pKa = 4.07DD142 pKa = 4.37YY143 pKa = 11.68STASGNYY150 pKa = 9.14ILATPTDD157 pKa = 3.89TVSTDD162 pKa = 3.24EE163 pKa = 4.87NSGIWFLSLATGSPAAGLTLPDD185 pKa = 4.05LPP187 pKa = 5.97

Molecular weight: 19.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8IAA0|A0A4Y8IAA0_9BACT 5'-nucleotidase SurE OS=Candidatus Marinimicrobia bacterium MT.SAG.4 OX=2552981 GN=surE PE=3 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.02KK4 pKa = 10.25SWIAKK9 pKa = 7.53QKK11 pKa = 8.76KK12 pKa = 4.13TQKK15 pKa = 10.16FAVRR19 pKa = 11.84EE20 pKa = 3.92YY21 pKa = 11.02NRR23 pKa = 11.84CQRR26 pKa = 11.84CGRR29 pKa = 11.84ARR31 pKa = 11.84SYY33 pKa = 10.81YY34 pKa = 10.18RR35 pKa = 11.84RR36 pKa = 11.84FGLCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.87LALKK50 pKa = 10.94GEE52 pKa = 4.37IPGITKK58 pKa = 10.6ASWW61 pKa = 2.73

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1311

0

1311

408134

25

2049

311.3

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.637 ± 0.07

0.628 ± 0.022

5.853 ± 0.052

7.231 ± 0.077

4.579 ± 0.056

7.359 ± 0.058

1.655 ± 0.025

8.293 ± 0.056

6.582 ± 0.074

9.757 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.033

4.678 ± 0.056

3.602 ± 0.04

2.447 ± 0.034

4.601 ± 0.054

7.466 ± 0.06

5.171 ± 0.047

6.356 ± 0.055

1.052 ± 0.024

3.523 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski