Capybara microvirus Cap3_SP_367
Average proteome isoelectric point is 5.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4U3|A0A4P8W4U3_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_367 OX=2585436 PE=4 SV=1
MM1 pKa = 7.69 ANGFDD6 pKa = 4.04 SLHH9 pKa = 6.08 ITDD12 pKa = 4.88 AVPNKK17 pKa = 10.66 SKK19 pKa = 10.91 FDD21 pKa = 3.73 LSRR24 pKa = 11.84 PHH26 pKa = 6.43 LTTMDD31 pKa = 3.67 FGEE34 pKa = 4.25 ITPVFCEE41 pKa = 4.01 EE42 pKa = 4.56 GLPGDD47 pKa = 4.21 KK48 pKa = 9.92 FTVDD52 pKa = 2.99 MTAFARR58 pKa = 11.84 LAPMVFPTYY67 pKa = 10.61 GKK69 pKa = 10.45 CCLKK73 pKa = 10.66 SAAYY77 pKa = 9.91 YY78 pKa = 9.58 VDD80 pKa = 4.25 YY81 pKa = 11.0 YY82 pKa = 11.53 QLADD86 pKa = 3.98 DD87 pKa = 5.29 FEE89 pKa = 6.05 AFISGQTTYY98 pKa = 11.04 RR99 pKa = 11.84 GTTPQLRR106 pKa = 11.84 VISADD111 pKa = 3.92 GILDD115 pKa = 4.49 LLTSNPSSEE124 pKa = 4.11 FFNDD128 pKa = 3.4 LSTEE132 pKa = 3.76 IANPSSEE139 pKa = 4.18 SEE141 pKa = 3.92 YY142 pKa = 11.23 DD143 pKa = 3.26 FTFDD147 pKa = 3.15 EE148 pKa = 5.25 DD149 pKa = 5.55 GIILYY154 pKa = 10.41 YY155 pKa = 10.66 KK156 pKa = 8.59 FTRR159 pKa = 11.84 KK160 pKa = 9.6 GRR162 pKa = 11.84 WVFKK166 pKa = 9.94 CLRR169 pKa = 11.84 SLGYY173 pKa = 10.15 DD174 pKa = 2.83 IPTQRR179 pKa = 11.84 GDD181 pKa = 3.35 SSVFSAMPLLAFFKK195 pKa = 10.61 AYY197 pKa = 10.31 NDD199 pKa = 3.55 YY200 pKa = 10.0 MALNSIQNMNITSQMLQAIKK220 pKa = 10.67 NKK222 pKa = 9.24 VSASVTVYY230 pKa = 9.99 PYY232 pKa = 10.71 EE233 pKa = 5.23 GGAGSEE239 pKa = 5.01 GYY241 pKa = 7.87 TVSYY245 pKa = 10.41 NGSNVISGDD254 pKa = 3.52 VIRR257 pKa = 11.84 AMFDD261 pKa = 3.76 DD262 pKa = 4.75 IRR264 pKa = 11.84 LCFDD268 pKa = 3.23 NDD270 pKa = 3.72 YY271 pKa = 9.93 VTSLWSTANQAYY283 pKa = 9.2 PGLSQNITTPTLNGVQMNQSVVKK306 pKa = 10.03 DD307 pKa = 3.55 WNNTSVSGISGSISQRR323 pKa = 11.84 SLDD326 pKa = 3.81 VLRR329 pKa = 11.84 MFDD332 pKa = 2.72 NWVRR336 pKa = 11.84 RR337 pKa = 11.84 NNYY340 pKa = 9.41 VGSKK344 pKa = 10.15 DD345 pKa = 3.46 VNQIYY350 pKa = 10.68 ARR352 pKa = 11.84 FGIHH356 pKa = 6.95 PEE358 pKa = 3.81 NFKK361 pKa = 11.09 AKK363 pKa = 8.03 YY364 pKa = 9.34 SHH366 pKa = 6.86 MIGKK370 pKa = 9.16 SEE372 pKa = 4.48 DD373 pKa = 3.86 CIQVGDD379 pKa = 3.87 VTSTSDD385 pKa = 3.26 TYY387 pKa = 11.59 QDD389 pKa = 3.35 ASNGAVIGSYY399 pKa = 9.82 AGKK402 pKa = 10.45 GIASGDD408 pKa = 3.77 SKK410 pKa = 10.89 FVYY413 pKa = 10.58 SPDD416 pKa = 3.31 EE417 pKa = 4.29 FGCLIILSWISVRR430 pKa = 11.84 PMYY433 pKa = 10.28 TRR435 pKa = 11.84 GFQRR439 pKa = 11.84 HH440 pKa = 5.61 TLRR443 pKa = 11.84 QSPTDD448 pKa = 4.11 FYY450 pKa = 11.03 TPEE453 pKa = 3.92 FDD455 pKa = 4.09 GVGGNAVSYY464 pKa = 11.15 RR465 pKa = 11.84 EE466 pKa = 3.74 ITVPKK471 pKa = 9.7 TVDD474 pKa = 3.67 DD475 pKa = 4.31 EE476 pKa = 5.36 SMGYY480 pKa = 10.51 YY481 pKa = 10.13 NDD483 pKa = 3.49 GVGFNEE489 pKa = 4.97 RR490 pKa = 11.84 YY491 pKa = 9.67 CEE493 pKa = 3.92 YY494 pKa = 10.43 RR495 pKa = 11.84 DD496 pKa = 3.61 SSKK499 pKa = 11.07 KK500 pKa = 10.83 GIVSGDD506 pKa = 3.5 FLLDD510 pKa = 3.13 TGMDD514 pKa = 2.96 AWHH517 pKa = 7.23 FGRR520 pKa = 11.84 DD521 pKa = 3.48 LDD523 pKa = 5.11 SLYY526 pKa = 10.65 TSHH529 pKa = 7.19 NLGAQTYY536 pKa = 9.4 ALNTYY541 pKa = 7.92 GVPGSVSEE549 pKa = 4.13 YY550 pKa = 11.22 DD551 pKa = 4.08 RR552 pKa = 11.84 IFNNAGSVAGRR563 pKa = 11.84 DD564 pKa = 3.22 HH565 pKa = 6.91 FFISFYY571 pKa = 11.05 FNVQAIRR578 pKa = 11.84 PIKK581 pKa = 10.4 NMNAVADD588 pKa = 4.59 LGSGDD593 pKa = 3.64 INISKK598 pKa = 10.36 VGNEE602 pKa = 4.03 VNN604 pKa = 3.5
Molecular weight: 67.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.583
IPC2_protein 4.736
IPC_protein 4.724
Toseland 4.558
ProMoST 4.863
Dawson 4.711
Bjellqvist 4.851
Wikipedia 4.622
Rodwell 4.571
Grimsley 4.469
Solomon 4.711
Lehninger 4.66
Nozaki 4.813
DTASelect 5.067
Thurlkill 4.584
EMBOSS 4.647
Sillero 4.863
Patrickios 3.172
IPC_peptide 4.711
IPC2_peptide 4.851
IPC2.peptide.svr19 4.81
Protein with the highest isoelectric point:
>tr|A0A4P8W584|A0A4P8W584_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_367 OX=2585436 PE=4 SV=1
MM1 pKa = 8.47 DD2 pKa = 4.71 ILNRR6 pKa = 11.84 NKK8 pKa = 10.48 NFIPGKK14 pKa = 10.21 DD15 pKa = 2.96 KK16 pKa = 10.93 VTFSLPDD23 pKa = 3.4 MKK25 pKa = 10.89 SYY27 pKa = 10.32 IYY29 pKa = 9.6 SQRR32 pKa = 11.84 KK33 pKa = 7.08 IEE35 pKa = 4.13 NYY37 pKa = 7.38 EE38 pKa = 3.81 TLAYY42 pKa = 8.62 YY43 pKa = 8.79 QWKK46 pKa = 8.1 EE47 pKa = 3.92 TVDD50 pKa = 4.06 NDD52 pKa = 4.1 GQCYY56 pKa = 9.4 FYY58 pKa = 10.91 TLTYY62 pKa = 10.49 NDD64 pKa = 3.18 NALPKK69 pKa = 10.29 FNGVPCFDD77 pKa = 4.75 YY78 pKa = 11.04 EE79 pKa = 4.47 DD80 pKa = 3.81 HH81 pKa = 8.1 DD82 pKa = 4.32 KK83 pKa = 11.14 FLDD86 pKa = 3.83 VLSHH90 pKa = 6.87 KK91 pKa = 10.38 LLRR94 pKa = 11.84 SYY96 pKa = 8.75 GTKK99 pKa = 10.32 LKK101 pKa = 10.8 YY102 pKa = 10.52 FSTTEE107 pKa = 3.71 RR108 pKa = 11.84 GEE110 pKa = 4.28 GKK112 pKa = 10.49 GFRR115 pKa = 11.84 GKK117 pKa = 10.79 GNNPHH122 pKa = 5.14 YY123 pKa = 11.09 HH124 pKa = 5.78 MLFFLTNANNPNYY137 pKa = 9.46 SYY139 pKa = 11.53 KK140 pKa = 10.64 KK141 pKa = 9.81 IRR143 pKa = 11.84 PTDD146 pKa = 3.7 FCAIVKK152 pKa = 9.61 NYY154 pKa = 8.0 WQGTTKK160 pKa = 10.38 EE161 pKa = 4.31 GKK163 pKa = 9.55 NVAQHH168 pKa = 5.31 YY169 pKa = 9.35 KK170 pKa = 10.6 YY171 pKa = 10.79 GIAAPGDD178 pKa = 3.38 NFGLVKK184 pKa = 10.54 SPQAVTYY191 pKa = 9.27 CCKK194 pKa = 10.43 YY195 pKa = 8.1 VCKK198 pKa = 10.5 DD199 pKa = 2.79 AAEE202 pKa = 4.13 RR203 pKa = 11.84 SNYY206 pKa = 9.79 DD207 pKa = 3.25 SQVHH211 pKa = 5.65 YY212 pKa = 10.5 IVSKK216 pKa = 9.58 TINEE220 pKa = 4.41 TLSSVHH226 pKa = 5.66 IQEE229 pKa = 4.64 EE230 pKa = 4.47 FLLSQVFSRR239 pKa = 11.84 DD240 pKa = 3.26 GLSMKK245 pKa = 10.3 EE246 pKa = 4.03 GLSMIKK252 pKa = 10.1 DD253 pKa = 3.61 CYY255 pKa = 10.94 LRR257 pKa = 11.84 TEE259 pKa = 4.45 INSKK263 pKa = 8.88 DD264 pKa = 3.63 TPSTISYY271 pKa = 8.89 WFIIHH276 pKa = 6.62 SGLYY280 pKa = 10.32 DD281 pKa = 3.55 EE282 pKa = 5.19 FRR284 pKa = 11.84 SYY286 pKa = 11.13 CILEE290 pKa = 4.4 CQDD293 pKa = 4.0 KK294 pKa = 10.58 IRR296 pKa = 11.84 EE297 pKa = 4.15 RR298 pKa = 11.84 VNEE301 pKa = 3.61 FRR303 pKa = 11.84 RR304 pKa = 11.84 RR305 pKa = 11.84 YY306 pKa = 8.23 CGKK309 pKa = 9.57 VRR311 pKa = 11.84 HH312 pKa = 6.45 SNDD315 pKa = 2.9 FGLSGLSTIKK325 pKa = 10.83 DD326 pKa = 3.56 DD327 pKa = 3.58 MNPRR331 pKa = 11.84 VLFPSKK337 pKa = 10.18 KK338 pKa = 8.35 GYY340 pKa = 10.15 KK341 pKa = 9.42 YY342 pKa = 10.51 RR343 pKa = 11.84 PLPLYY348 pKa = 10.03 YY349 pKa = 9.94 YY350 pKa = 9.55 RR351 pKa = 11.84 KK352 pKa = 9.05 KK353 pKa = 10.57 YY354 pKa = 10.48 CVVDD358 pKa = 3.37 YY359 pKa = 11.34 YY360 pKa = 10.95 MQGDD364 pKa = 3.83 KK365 pKa = 10.7 KK366 pKa = 10.61 IPYY369 pKa = 9.22 YY370 pKa = 10.45 RR371 pKa = 11.84 LNDD374 pKa = 3.83 LGVKK378 pKa = 8.03 YY379 pKa = 9.37 TNFKK383 pKa = 10.5 LDD385 pKa = 3.45 SLIDD389 pKa = 3.72 SKK391 pKa = 11.48 KK392 pKa = 8.81 ITTEE396 pKa = 3.66 KK397 pKa = 10.87 ALSFVDD403 pKa = 4.01 KK404 pKa = 10.93 LGSGYY409 pKa = 10.27 FYY411 pKa = 11.17 SEE413 pKa = 4.02 QFKK416 pKa = 11.11 SLGMPEE422 pKa = 4.66 NKK424 pKa = 9.34 VKK426 pKa = 10.96 YY427 pKa = 10.56 LIGDD431 pKa = 4.36 LNSSIKK437 pKa = 10.56 DD438 pKa = 3.43 NYY440 pKa = 9.77 SVYY443 pKa = 10.88 SVVYY447 pKa = 10.14 KK448 pKa = 10.76 NRR450 pKa = 11.84 SLSGLEE456 pKa = 4.97 DD457 pKa = 3.28 IDD459 pKa = 5.73 RR460 pKa = 11.84 PLDD463 pKa = 3.68 MYY465 pKa = 11.69 NDD467 pKa = 3.01 WCYY470 pKa = 11.3 FNTPVIKK477 pKa = 10.2 EE478 pKa = 4.37 DD479 pKa = 3.36 NSNTYY484 pKa = 8.04 TAYY487 pKa = 10.7 AFLKK491 pKa = 10.73 SFTRR495 pKa = 11.84 YY496 pKa = 10.74 DD497 pKa = 3.44 FTDD500 pKa = 4.19 DD501 pKa = 4.29 GLVHH505 pKa = 6.44 TNSLTYY511 pKa = 9.93 RR512 pKa = 11.84 YY513 pKa = 9.54 HH514 pKa = 7.78 KK515 pKa = 10.76 DD516 pKa = 3.3 FEE518 pKa = 4.66 CFLEE522 pKa = 4.53 YY523 pKa = 10.42 FDD525 pKa = 5.93 LLDD528 pKa = 4.97 SIIDD532 pKa = 3.83 YY533 pKa = 11.39 YY534 pKa = 11.0 VMCDD538 pKa = 3.24 SLEE541 pKa = 4.16 KK542 pKa = 10.93 EE543 pKa = 4.11 NIARR547 pKa = 11.84 QWRR550 pKa = 11.84 DD551 pKa = 2.71 TRR553 pKa = 11.84 NVINSIKK560 pKa = 10.17 HH561 pKa = 4.89 PEE563 pKa = 4.1 YY564 pKa = 10.85 YY565 pKa = 10.79 GLAVV569 pKa = 3.44
Molecular weight: 67.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.486
IPC2_protein 7.717
IPC_protein 7.512
Toseland 7.176
ProMoST 8.083
Dawson 8.244
Bjellqvist 8.536
Wikipedia 8.185
Rodwell 8.273
Grimsley 7.205
Solomon 8.287
Lehninger 8.287
Nozaki 8.58
DTASelect 8.302
Thurlkill 8.346
EMBOSS 8.419
Sillero 8.639
Patrickios 3.452
IPC_peptide 8.273
IPC2_peptide 7.41
IPC2.peptide.svr19 7.765
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1648
98
604
412.0
46.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.886 ± 1.797
1.578 ± 0.381
7.464 ± 0.6
5.34 ± 1.151
4.672 ± 0.804
6.25 ± 0.918
1.335 ± 0.38
5.825 ± 0.323
6.978 ± 1.359
6.675 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.367 ± 0.249
6.553 ± 0.33
3.459 ± 0.183
3.762 ± 0.984
4.126 ± 0.324
8.495 ± 0.862
5.4 ± 0.384
6.189 ± 0.447
1.032 ± 0.166
6.614 ± 1.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here