Amino acid dipepetide frequency for Aeromonas phage MJG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.433AlaAla: 9.433 ± 1.432
0.387AlaCys: 0.387 ± 0.185
5.103AlaAsp: 5.103 ± 0.37
6.34AlaGlu: 6.34 ± 0.777
3.015AlaPhe: 3.015 ± 0.362
6.881AlaGly: 6.881 ± 0.898
1.469AlaHis: 1.469 ± 0.29
3.788AlaIle: 3.788 ± 0.616
6.417AlaLys: 6.417 ± 0.797
7.036AlaLeu: 7.036 ± 0.722
3.325AlaMet: 3.325 ± 0.913
3.557AlaAsn: 3.557 ± 0.553
3.634AlaPro: 3.634 ± 0.713
4.175AlaGln: 4.175 ± 0.886
4.871AlaArg: 4.871 ± 0.637
4.562AlaSer: 4.562 ± 0.711
5.18AlaThr: 5.18 ± 0.653
7.345AlaVal: 7.345 ± 0.693
0.619AlaTrp: 0.619 ± 0.184
2.938AlaTyr: 2.938 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.541CysAla: 0.541 ± 0.167
0.077CysCys: 0.077 ± 0.072
0.619CysAsp: 0.619 ± 0.251
0.619CysGlu: 0.619 ± 0.202
0.541CysPhe: 0.541 ± 0.169
0.155CysGly: 0.155 ± 0.101
0.077CysHis: 0.077 ± 0.07
0.696CysIle: 0.696 ± 0.249
0.928CysLys: 0.928 ± 0.318
0.928CysLeu: 0.928 ± 0.323
0.232CysMet: 0.232 ± 0.136
0.464CysAsn: 0.464 ± 0.168
0.387CysPro: 0.387 ± 0.145
0.619CysGln: 0.619 ± 0.2
0.85CysArg: 0.85 ± 0.314
0.619CysSer: 0.619 ± 0.246
0.696CysThr: 0.696 ± 0.238
0.619CysVal: 0.619 ± 0.219
0.0CysTrp: 0.0 ± 0.0
0.232CysTyr: 0.232 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
6.417AspAla: 6.417 ± 0.554
0.464AspCys: 0.464 ± 0.23
3.866AspAsp: 3.866 ± 0.64
4.252AspGlu: 4.252 ± 0.625
2.088AspPhe: 2.088 ± 0.474
5.103AspGly: 5.103 ± 0.835
1.237AspHis: 1.237 ± 0.311
4.484AspIle: 4.484 ± 0.746
5.026AspLys: 5.026 ± 0.712
5.103AspLeu: 5.103 ± 0.635
2.088AspMet: 2.088 ± 0.341
2.706AspAsn: 2.706 ± 0.383
2.861AspPro: 2.861 ± 0.504
0.619AspGln: 0.619 ± 0.176
1.933AspArg: 1.933 ± 0.403
4.02AspSer: 4.02 ± 0.688
3.634AspThr: 3.634 ± 0.631
3.866AspVal: 3.866 ± 0.544
1.005AspTrp: 1.005 ± 0.309
3.093AspTyr: 3.093 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
6.726GluAla: 6.726 ± 0.883
0.928GluCys: 0.928 ± 0.289
4.098GluAsp: 4.098 ± 0.6
6.185GluGlu: 6.185 ± 1.001
2.783GluPhe: 2.783 ± 0.405
4.098GluGly: 4.098 ± 0.573
1.778GluHis: 1.778 ± 0.325
3.247GluIle: 3.247 ± 0.431
3.325GluLys: 3.325 ± 0.471
7.19GluLeu: 7.19 ± 0.774
2.088GluMet: 2.088 ± 0.308
1.778GluAsn: 1.778 ± 0.357
2.474GluPro: 2.474 ± 0.403
3.479GluGln: 3.479 ± 0.622
4.407GluArg: 4.407 ± 0.555
3.866GluSer: 3.866 ± 0.586
3.634GluThr: 3.634 ± 0.591
4.948GluVal: 4.948 ± 0.564
1.546GluTrp: 1.546 ± 0.445
2.319GluTyr: 2.319 ± 0.535
0.0GluXaa: 0.0 ± 0.0
Phe
2.938PheAla: 2.938 ± 0.485
0.387PheCys: 0.387 ± 0.147
3.557PheAsp: 3.557 ± 0.464
1.933PheGlu: 1.933 ± 0.351
2.397PhePhe: 2.397 ± 0.425
2.474PheGly: 2.474 ± 0.48
1.005PheHis: 1.005 ± 0.313
1.701PheIle: 1.701 ± 0.301
2.165PheLys: 2.165 ± 0.319
2.629PheLeu: 2.629 ± 0.405
1.082PheMet: 1.082 ± 0.28
2.783PheAsn: 2.783 ± 0.583
2.319PhePro: 2.319 ± 0.449
1.16PheGln: 1.16 ± 0.292
2.474PheArg: 2.474 ± 0.394
2.551PheSer: 2.551 ± 0.392
2.242PheThr: 2.242 ± 0.435
2.088PheVal: 2.088 ± 0.392
0.464PheTrp: 0.464 ± 0.192
1.082PheTyr: 1.082 ± 0.239
0.0PheXaa: 0.0 ± 0.0
Gly
6.031GlyAla: 6.031 ± 0.969
0.696GlyCys: 0.696 ± 0.261
5.412GlyAsp: 5.412 ± 0.773
5.18GlyGlu: 5.18 ± 0.557
3.247GlyPhe: 3.247 ± 0.501
4.794GlyGly: 4.794 ± 0.76
1.933GlyHis: 1.933 ± 0.388
3.325GlyIle: 3.325 ± 0.366
5.567GlyLys: 5.567 ± 0.765
4.716GlyLeu: 4.716 ± 0.684
2.242GlyMet: 2.242 ± 0.343
4.33GlyAsn: 4.33 ± 0.597
0.773GlyPro: 0.773 ± 0.209
3.015GlyGln: 3.015 ± 0.504
4.562GlyArg: 4.562 ± 0.556
4.252GlySer: 4.252 ± 0.356
3.402GlyThr: 3.402 ± 0.445
4.794GlyVal: 4.794 ± 0.586
1.392GlyTrp: 1.392 ± 0.324
2.861GlyTyr: 2.861 ± 0.429
0.0GlyXaa: 0.0 ± 0.0
His
1.546HisAla: 1.546 ± 0.328
0.155HisCys: 0.155 ± 0.108
1.933HisAsp: 1.933 ± 0.3
1.082HisGlu: 1.082 ± 0.288
0.85HisPhe: 0.85 ± 0.264
1.624HisGly: 1.624 ± 0.291
0.387HisHis: 0.387 ± 0.149
0.85HisIle: 0.85 ± 0.283
1.546HisLys: 1.546 ± 0.397
2.474HisLeu: 2.474 ± 0.505
1.082HisMet: 1.082 ± 0.323
1.16HisAsn: 1.16 ± 0.267
1.082HisPro: 1.082 ± 0.226
0.696HisGln: 0.696 ± 0.224
1.237HisArg: 1.237 ± 0.279
1.082HisSer: 1.082 ± 0.303
0.773HisThr: 0.773 ± 0.292
1.314HisVal: 1.314 ± 0.275
0.232HisTrp: 0.232 ± 0.143
1.546HisTyr: 1.546 ± 0.227
0.0HisXaa: 0.0 ± 0.0
Ile
5.18IleAla: 5.18 ± 0.423
0.387IleCys: 0.387 ± 0.216
3.325IleAsp: 3.325 ± 0.452
3.557IleGlu: 3.557 ± 0.509
1.314IlePhe: 1.314 ± 0.37
3.788IleGly: 3.788 ± 0.482
1.314IleHis: 1.314 ± 0.271
1.701IleIle: 1.701 ± 0.482
3.325IleLys: 3.325 ± 0.42
2.861IleLeu: 2.861 ± 0.411
1.005IleMet: 1.005 ± 0.245
2.242IleAsn: 2.242 ± 0.378
2.706IlePro: 2.706 ± 0.399
2.474IleGln: 2.474 ± 0.38
3.015IleArg: 3.015 ± 0.465
2.165IleSer: 2.165 ± 0.417
2.938IleThr: 2.938 ± 0.497
4.02IleVal: 4.02 ± 0.554
0.696IleTrp: 0.696 ± 0.325
1.469IleTyr: 1.469 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
6.34LysAla: 6.34 ± 0.866
0.619LysCys: 0.619 ± 0.24
3.943LysAsp: 3.943 ± 0.746
3.557LysGlu: 3.557 ± 0.53
2.474LysPhe: 2.474 ± 0.413
4.484LysGly: 4.484 ± 0.802
1.392LysHis: 1.392 ± 0.405
3.325LysIle: 3.325 ± 0.437
3.093LysLys: 3.093 ± 0.504
5.876LysLeu: 5.876 ± 0.659
1.856LysMet: 1.856 ± 0.372
2.629LysAsn: 2.629 ± 0.479
3.325LysPro: 3.325 ± 0.408
2.088LysGln: 2.088 ± 0.307
3.711LysArg: 3.711 ± 0.516
2.861LysSer: 2.861 ± 0.596
3.634LysThr: 3.634 ± 0.418
4.098LysVal: 4.098 ± 0.423
0.232LysTrp: 0.232 ± 0.123
2.551LysTyr: 2.551 ± 0.436
0.0LysXaa: 0.0 ± 0.0
Leu
6.263LeuAla: 6.263 ± 0.787
0.928LeuCys: 0.928 ± 0.256
5.103LeuAsp: 5.103 ± 0.394
6.572LeuGlu: 6.572 ± 0.621
2.783LeuPhe: 2.783 ± 0.417
5.644LeuGly: 5.644 ± 0.59
1.778LeuHis: 1.778 ± 0.307
3.866LeuIle: 3.866 ± 0.537
4.407LeuLys: 4.407 ± 0.895
4.33LeuLeu: 4.33 ± 0.496
2.938LeuMet: 2.938 ± 0.393
3.634LeuAsn: 3.634 ± 0.554
3.866LeuPro: 3.866 ± 0.473
3.325LeuGln: 3.325 ± 0.409
4.252LeuArg: 4.252 ± 0.572
4.484LeuSer: 4.484 ± 0.727
5.567LeuThr: 5.567 ± 0.749
4.562LeuVal: 4.562 ± 0.57
0.696LeuTrp: 0.696 ± 0.279
2.397LeuTyr: 2.397 ± 0.486
0.0LeuXaa: 0.0 ± 0.0
Met
3.325MetAla: 3.325 ± 0.589
0.232MetCys: 0.232 ± 0.12
2.01MetAsp: 2.01 ± 0.345
1.933MetGlu: 1.933 ± 0.47
1.624MetPhe: 1.624 ± 0.378
1.778MetGly: 1.778 ± 0.392
0.619MetHis: 0.619 ± 0.221
1.392MetIle: 1.392 ± 0.351
2.088MetLys: 2.088 ± 0.372
2.938MetLeu: 2.938 ± 0.48
1.005MetMet: 1.005 ± 0.269
1.546MetAsn: 1.546 ± 0.417
1.469MetPro: 1.469 ± 0.318
1.237MetGln: 1.237 ± 0.358
1.856MetArg: 1.856 ± 0.376
2.165MetSer: 2.165 ± 0.363
2.242MetThr: 2.242 ± 0.357
1.778MetVal: 1.778 ± 0.278
0.387MetTrp: 0.387 ± 0.149
0.928MetTyr: 0.928 ± 0.295
0.0MetXaa: 0.0 ± 0.0
Asn
3.247AsnAla: 3.247 ± 0.485
0.309AsnCys: 0.309 ± 0.133
2.474AsnAsp: 2.474 ± 0.358
2.629AsnGlu: 2.629 ± 0.363
1.469AsnPhe: 1.469 ± 0.341
4.33AsnGly: 4.33 ± 0.639
1.005AsnHis: 1.005 ± 0.258
2.088AsnIle: 2.088 ± 0.272
2.783AsnLys: 2.783 ± 0.476
4.098AsnLeu: 4.098 ± 0.535
1.314AsnMet: 1.314 ± 0.331
1.933AsnAsn: 1.933 ± 0.382
3.17AsnPro: 3.17 ± 0.492
2.165AsnGln: 2.165 ± 0.269
2.397AsnArg: 2.397 ± 0.472
2.474AsnSer: 2.474 ± 0.471
2.706AsnThr: 2.706 ± 0.471
2.861AsnVal: 2.861 ± 0.572
0.619AsnTrp: 0.619 ± 0.153
2.165AsnTyr: 2.165 ± 0.41
0.0AsnXaa: 0.0 ± 0.0
Pro
3.711ProAla: 3.711 ± 0.587
0.309ProCys: 0.309 ± 0.171
2.938ProAsp: 2.938 ± 0.479
3.943ProGlu: 3.943 ± 0.629
1.856ProPhe: 1.856 ± 0.434
0.232ProGly: 0.232 ± 0.134
0.928ProHis: 0.928 ± 0.316
2.165ProIle: 2.165 ± 0.444
2.706ProLys: 2.706 ± 0.41
2.242ProLeu: 2.242 ± 0.41
1.16ProMet: 1.16 ± 0.299
1.933ProAsn: 1.933 ± 0.339
1.16ProPro: 1.16 ± 0.269
1.933ProGln: 1.933 ± 0.396
1.392ProArg: 1.392 ± 0.243
2.629ProSer: 2.629 ± 0.376
2.551ProThr: 2.551 ± 0.566
3.634ProVal: 3.634 ± 0.548
1.005ProTrp: 1.005 ± 0.262
2.01ProTyr: 2.01 ± 0.517
0.0ProXaa: 0.0 ± 0.0
Gln
4.716GlnAla: 4.716 ± 0.672
0.464GlnCys: 0.464 ± 0.187
2.01GlnAsp: 2.01 ± 0.397
3.17GlnGlu: 3.17 ± 0.507
1.701GlnPhe: 1.701 ± 0.277
2.861GlnGly: 2.861 ± 0.452
1.082GlnHis: 1.082 ± 0.232
1.933GlnIle: 1.933 ± 0.4
1.856GlnLys: 1.856 ± 0.306
3.17GlnLeu: 3.17 ± 0.545
2.165GlnMet: 2.165 ± 0.416
0.928GlnAsn: 0.928 ± 0.209
0.928GlnPro: 0.928 ± 0.274
2.397GlnGln: 2.397 ± 0.661
2.242GlnArg: 2.242 ± 0.493
2.629GlnSer: 2.629 ± 0.472
2.165GlnThr: 2.165 ± 0.369
2.397GlnVal: 2.397 ± 0.467
0.387GlnTrp: 0.387 ± 0.184
1.005GlnTyr: 1.005 ± 0.269
0.0GlnXaa: 0.0 ± 0.0
Arg
4.484ArgAla: 4.484 ± 0.644
0.464ArgCys: 0.464 ± 0.187
3.325ArgAsp: 3.325 ± 0.625
3.015ArgGlu: 3.015 ± 0.673
2.01ArgPhe: 2.01 ± 0.417
4.871ArgGly: 4.871 ± 0.682
0.928ArgHis: 0.928 ± 0.206
2.551ArgIle: 2.551 ± 0.482
2.551ArgLys: 2.551 ± 0.43
4.716ArgLeu: 4.716 ± 0.468
2.397ArgMet: 2.397 ± 0.31
2.551ArgAsn: 2.551 ± 0.523
1.392ArgPro: 1.392 ± 0.38
2.397ArgGln: 2.397 ± 0.402
2.397ArgArg: 2.397 ± 0.458
3.247ArgSer: 3.247 ± 0.477
3.015ArgThr: 3.015 ± 0.583
4.33ArgVal: 4.33 ± 0.506
0.928ArgTrp: 0.928 ± 0.184
2.088ArgTyr: 2.088 ± 0.327
0.0ArgXaa: 0.0 ± 0.0
Ser
4.948SerAla: 4.948 ± 0.781
0.541SerCys: 0.541 ± 0.179
3.634SerAsp: 3.634 ± 0.451
3.634SerGlu: 3.634 ± 0.507
2.319SerPhe: 2.319 ± 0.375
5.103SerGly: 5.103 ± 0.559
1.624SerHis: 1.624 ± 0.284
3.788SerIle: 3.788 ± 0.48
3.402SerLys: 3.402 ± 0.416
4.175SerLeu: 4.175 ± 0.511
1.933SerMet: 1.933 ± 0.397
2.783SerAsn: 2.783 ± 0.458
1.856SerPro: 1.856 ± 0.329
1.778SerGln: 1.778 ± 0.474
2.783SerArg: 2.783 ± 0.599
2.861SerSer: 2.861 ± 0.42
2.861SerThr: 2.861 ± 0.539
3.634SerVal: 3.634 ± 0.49
0.619SerTrp: 0.619 ± 0.224
1.624SerTyr: 1.624 ± 0.431
0.0SerXaa: 0.0 ± 0.0
Thr
4.252ThrAla: 4.252 ± 0.566
0.85ThrCys: 0.85 ± 0.284
3.557ThrAsp: 3.557 ± 0.647
4.562ThrGlu: 4.562 ± 0.543
2.242ThrPhe: 2.242 ± 0.442
5.644ThrGly: 5.644 ± 0.748
1.314ThrHis: 1.314 ± 0.271
2.551ThrIle: 2.551 ± 0.256
3.634ThrLys: 3.634 ± 0.625
4.098ThrLeu: 4.098 ± 0.521
1.469ThrMet: 1.469 ± 0.356
2.783ThrAsn: 2.783 ± 0.682
3.17ThrPro: 3.17 ± 0.479
1.933ThrGln: 1.933 ± 0.299
2.551ThrArg: 2.551 ± 0.333
2.861ThrSer: 2.861 ± 0.579
2.783ThrThr: 2.783 ± 0.45
3.325ThrVal: 3.325 ± 0.477
0.541ThrTrp: 0.541 ± 0.184
1.856ThrTyr: 1.856 ± 0.368
0.0ThrXaa: 0.0 ± 0.0
Val
6.263ValAla: 6.263 ± 0.924
1.082ValCys: 1.082 ± 0.332
3.943ValAsp: 3.943 ± 0.51
5.103ValGlu: 5.103 ± 0.625
2.861ValPhe: 2.861 ± 0.386
5.18ValGly: 5.18 ± 0.861
1.856ValHis: 1.856 ± 0.375
3.557ValIle: 3.557 ± 0.556
4.562ValLys: 4.562 ± 0.539
4.948ValLeu: 4.948 ± 0.782
1.469ValMet: 1.469 ± 0.327
3.943ValAsn: 3.943 ± 0.494
1.856ValPro: 1.856 ± 0.402
2.474ValGln: 2.474 ± 0.814
3.557ValArg: 3.557 ± 0.566
3.557ValSer: 3.557 ± 0.589
2.861ValThr: 2.861 ± 0.563
5.103ValVal: 5.103 ± 0.516
1.237ValTrp: 1.237 ± 0.32
2.242ValTyr: 2.242 ± 0.42
0.0ValXaa: 0.0 ± 0.0
Trp
0.85TrpAla: 0.85 ± 0.309
0.309TrpCys: 0.309 ± 0.131
0.696TrpAsp: 0.696 ± 0.243
1.314TrpGlu: 1.314 ± 0.3
0.541TrpPhe: 0.541 ± 0.198
1.16TrpGly: 1.16 ± 0.275
0.387TrpHis: 0.387 ± 0.181
0.619TrpIle: 0.619 ± 0.22
1.082TrpLys: 1.082 ± 0.337
0.773TrpLeu: 0.773 ± 0.252
0.387TrpMet: 0.387 ± 0.175
0.387TrpAsn: 0.387 ± 0.159
0.696TrpPro: 0.696 ± 0.333
0.464TrpGln: 0.464 ± 0.172
0.85TrpArg: 0.85 ± 0.293
0.773TrpSer: 0.773 ± 0.326
0.85TrpThr: 0.85 ± 0.266
0.85TrpVal: 0.85 ± 0.253
0.387TrpTrp: 0.387 ± 0.196
0.464TrpTyr: 0.464 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.938TyrAla: 2.938 ± 0.527
0.309TyrCys: 0.309 ± 0.131
2.319TyrAsp: 2.319 ± 0.462
2.319TyrGlu: 2.319 ± 0.536
1.314TyrPhe: 1.314 ± 0.295
2.319TyrGly: 2.319 ± 0.382
0.541TyrHis: 0.541 ± 0.159
1.856TyrIle: 1.856 ± 0.443
1.624TyrLys: 1.624 ± 0.294
3.093TyrLeu: 3.093 ± 0.351
1.237TyrMet: 1.237 ± 0.393
2.165TyrAsn: 2.165 ± 0.39
1.314TyrPro: 1.314 ± 0.362
1.778TyrGln: 1.778 ± 0.365
2.319TyrArg: 2.319 ± 0.394
2.242TyrSer: 2.242 ± 0.352
2.242TyrThr: 2.242 ± 0.367
2.088TyrVal: 2.088 ± 0.435
0.85TyrTrp: 0.85 ± 0.306
0.387TyrTyr: 0.387 ± 0.156
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 35 proteins (12935 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski