Red sea bream iridovirus (RSIV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Megalocytivirus; Infectious spleen and kidney necrosis virus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218PFJ1|A0A218PFJ1_RSIV ORF004 OS=Red sea bream iridovirus OX=65424 GN=ORF021 PE=4 SV=1
MM1 pKa = 7.9SSEE4 pKa = 3.94LTLWRR9 pKa = 11.84SVADD13 pKa = 3.76ACGVTYY19 pKa = 9.99PLVRR23 pKa = 11.84SYY25 pKa = 11.16VAALEE30 pKa = 4.49SGCDD34 pKa = 3.15PGTTFRR40 pKa = 11.84NYY42 pKa = 7.67WSMRR46 pKa = 11.84RR47 pKa = 11.84AHH49 pKa = 6.78IMAGNPFEE57 pKa = 5.94DD58 pKa = 3.94DD59 pKa = 4.82DD60 pKa = 4.52YY61 pKa = 11.49FEE63 pKa = 4.54TMSGVVISMATVAEE77 pKa = 4.62HH78 pKa = 7.14CDD80 pKa = 3.65SLPDD84 pKa = 4.66LLATYY89 pKa = 9.95NAAAQTAPVFDD100 pKa = 4.61CPMLQGFLDD109 pKa = 3.58TVMQSEE115 pKa = 4.91LLQDD119 pKa = 3.07IHH121 pKa = 8.59RR122 pKa = 11.84DD123 pKa = 3.31VAAGGNSANLLNIISHH139 pKa = 7.07NISKK143 pKa = 10.46HH144 pKa = 5.93DD145 pKa = 3.91DD146 pKa = 4.12FIKK149 pKa = 10.62HH150 pKa = 5.68FSGNIIGYY158 pKa = 9.47LEE160 pKa = 4.12TMPVDD165 pKa = 4.11EE166 pKa = 5.35LAQMMEE172 pKa = 4.67VIKK175 pKa = 10.77EE176 pKa = 3.96MTGQGGDD183 pKa = 3.79EE184 pKa = 4.86LIQAIMAHH192 pKa = 6.14APVV195 pKa = 3.65

Molecular weight: 21.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1SVU3|F1SVU3_RSIV ORF010 OS=Red sea bream iridovirus OX=65424 GN=ORF 353R PE=4 SV=1
MM1 pKa = 7.21PQPIIFKK8 pKa = 9.06CTASPSALRR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 6.56TASPRR24 pKa = 11.84KK25 pKa = 9.33RR26 pKa = 11.84KK27 pKa = 10.14SPAKK31 pKa = 9.65RR32 pKa = 11.84RR33 pKa = 11.84SPAKK37 pKa = 9.51RR38 pKa = 11.84RR39 pKa = 11.84SPVKK43 pKa = 9.77RR44 pKa = 11.84RR45 pKa = 11.84SPVKK49 pKa = 9.81RR50 pKa = 11.84RR51 pKa = 11.84SPVKK55 pKa = 9.65RR56 pKa = 11.84RR57 pKa = 11.84SPARR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84SPARR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 3.46

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

35513

51

1282

301.0

33.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.633 ± 0.289

3.041 ± 0.214

6.155 ± 0.173

4.021 ± 0.205

3.019 ± 0.117

5.508 ± 0.166

3.675 ± 0.137

4.601 ± 0.142

3.41 ± 0.13

8.127 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.12 ± 0.147

3.717 ± 0.13

5.581 ± 0.284

3.855 ± 0.155

6.296 ± 0.273

5.66 ± 0.166

6.966 ± 0.209

8.532 ± 0.265

1.112 ± 0.067

3.97 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski