Staphylococcus phage StauST398-1
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M9NUK6|M9NUK6_9CAUD Uncharacterized protein OS=Staphylococcus phage StauST398-1 OX=1195068 GN=StauST398-1_0045 PE=4 SV=1
MM1 pKa = 7.51 NNRR4 pKa = 11.84 EE5 pKa = 4.1 QIEE8 pKa = 3.98 QSVISASAYY17 pKa = 10.04 NGNDD21 pKa = 3.29 TEE23 pKa = 4.52 GLLKK27 pKa = 10.49 EE28 pKa = 4.44 IEE30 pKa = 4.25 DD31 pKa = 4.16 VYY33 pKa = 11.47 KK34 pKa = 10.57 KK35 pKa = 10.68 ARR37 pKa = 11.84 AFDD40 pKa = 4.71 EE41 pKa = 4.26 ILEE44 pKa = 4.21 GLPNAMQDD52 pKa = 3.61 ALKK55 pKa = 10.63 EE56 pKa = 4.3 DD57 pKa = 3.43 IGLDD61 pKa = 3.48 EE62 pKa = 5.32 AVGIMTGQVVYY73 pKa = 10.25 KK74 pKa = 10.67 YY75 pKa = 10.65 EE76 pKa = 3.93 EE77 pKa = 4.06 EE78 pKa = 4.28 QEE80 pKa = 3.96 NEE82 pKa = 4.13 YY83 pKa = 11.4
Molecular weight: 9.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.227
IPC2_protein 4.088
IPC_protein 3.973
Toseland 3.808
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.783
Rodwell 3.808
Grimsley 3.719
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.139
Thurlkill 3.834
EMBOSS 3.808
Sillero 4.075
Patrickios 3.427
IPC_peptide 3.91
IPC2_peptide 4.062
IPC2.peptide.svr19 3.994
Protein with the highest isoelectric point:
>tr|M9NSU6|M9NSU6_9CAUD Complement inhibitor SCIN OS=Staphylococcus phage StauST398-1 OX=1195068 GN=scn PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.33 KK3 pKa = 10.53 AILTLSLIFITYY15 pKa = 9.82 YY16 pKa = 10.15 LTFKK20 pKa = 10.91 YY21 pKa = 9.6 MWIKK25 pKa = 8.96 EE26 pKa = 4.11 LKK28 pKa = 8.97 YY29 pKa = 10.89
Molecular weight: 3.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.128
IPC2_protein 9.414
IPC_protein 9.326
Toseland 9.736
ProMoST 9.589
Dawson 10.014
Bjellqvist 9.706
Wikipedia 10.204
Rodwell 10.365
Grimsley 10.101
Solomon 10.058
Lehninger 10.028
Nozaki 9.692
DTASelect 9.706
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.911
Patrickios 7.468
IPC_peptide 10.058
IPC2_peptide 8.287
IPC2.peptide.svr19 8.354
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13198
27
989
191.3
21.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.925 ± 0.359
0.485 ± 0.086
6.402 ± 0.271
7.1 ± 0.415
4.289 ± 0.256
5.811 ± 0.344
1.849 ± 0.166
7.857 ± 0.281
9.441 ± 0.346
7.706 ± 0.289
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.447 ± 0.169
6.539 ± 0.252
2.637 ± 0.173
3.963 ± 0.232
3.887 ± 0.206
6.077 ± 0.325
6.001 ± 0.234
6.069 ± 0.288
1.159 ± 0.148
4.357 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here