Torque teno mini virus 18
Average proteome isoelectric point is 7.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A173GQ21|A0A173GQ21_9VIRU ORF3 OS=Torque teno mini virus 18 OX=1859149 PE=4 SV=1
MM1 pKa = 7.49 SLTPVIYY8 pKa = 10.31 GPQALEE14 pKa = 4.09 LQWVNSIIHH23 pKa = 5.4 NHH25 pKa = 5.71 GLFCGCDD32 pKa = 3.27 TPIKK36 pKa = 10.13 HH37 pKa = 7.0 LEE39 pKa = 4.27 RR40 pKa = 11.84 ILNKK44 pKa = 10.3 RR45 pKa = 11.84 GTQLCLTRR53 pKa = 11.84 GDD55 pKa = 3.93 GEE57 pKa = 4.26 EE58 pKa = 5.36 DD59 pKa = 3.25 GKK61 pKa = 10.9 QDD63 pKa = 3.51 QEE65 pKa = 4.11 EE66 pKa = 4.63 DD67 pKa = 3.64 GGISPGDD74 pKa = 3.69 LEE76 pKa = 5.23 RR77 pKa = 11.84 LFAEE81 pKa = 5.34 DD82 pKa = 3.52 GDD84 pKa = 4.21 EE85 pKa = 4.23 EE86 pKa = 4.82 RR87 pKa = 11.84 GG88 pKa = 3.52
Molecular weight: 9.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.497
IPC2_protein 4.482
IPC_protein 4.38
Toseland 4.215
ProMoST 4.495
Dawson 4.329
Bjellqvist 4.482
Wikipedia 4.215
Rodwell 4.215
Grimsley 4.139
Solomon 4.329
Lehninger 4.279
Nozaki 4.444
DTASelect 4.596
Thurlkill 4.24
EMBOSS 4.228
Sillero 4.495
Patrickios 3.757
IPC_peptide 4.329
IPC2_peptide 4.482
IPC2.peptide.svr19 4.414
Protein with the highest isoelectric point:
>tr|A0A173GQ22|A0A173GQ22_9VIRU Capsid protein OS=Torque teno mini virus 18 OX=1859149 PE=3 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 SRR4 pKa = 11.84 VRR6 pKa = 11.84 QHH8 pKa = 5.92 QKK10 pKa = 7.94 NTSYY14 pKa = 8.98 THH16 pKa = 4.98 GTKK19 pKa = 10.45 NEE21 pKa = 4.07 DD22 pKa = 3.26 ALQNLQLKK30 pKa = 9.13 DD31 pKa = 4.13 LKK33 pKa = 10.55 PKK35 pKa = 10.1 YY36 pKa = 9.41 VQHH39 pKa = 7.22 LLQTLDD45 pKa = 2.92 TSTRR49 pKa = 11.84 GTKK52 pKa = 9.5 HH53 pKa = 5.9 KK54 pKa = 10.34 KK55 pKa = 4.85 QTRR58 pKa = 11.84 RR59 pKa = 11.84 HH60 pKa = 4.89 PRR62 pKa = 11.84 KK63 pKa = 9.16 KK64 pKa = 9.44 RR65 pKa = 11.84 KK66 pKa = 9.01 HH67 pKa = 5.13 RR68 pKa = 11.84 RR69 pKa = 11.84 SRR71 pKa = 11.84 TSSSSSSHH79 pKa = 6.14 SSNSSSSDD87 pKa = 3.2 SSDD90 pKa = 2.8
Molecular weight: 10.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.202
IPC2_protein 10.16
IPC_protein 11.33
Toseland 11.55
ProMoST 11.871
Dawson 11.564
Bjellqvist 11.462
Wikipedia 11.945
Rodwell 11.667
Grimsley 11.594
Solomon 11.945
Lehninger 11.871
Nozaki 11.535
DTASelect 11.462
Thurlkill 11.535
EMBOSS 12.003
Sillero 11.535
Patrickios 11.389
IPC_peptide 11.959
IPC2_peptide 10.862
IPC2.peptide.svr19 8.968
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
824
88
646
274.7
32.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.034 ± 0.703
1.699 ± 0.485
4.733 ± 1.277
5.218 ± 1.459
3.398 ± 1.203
4.733 ± 2.049
2.791 ± 1.597
5.947 ± 1.701
8.131 ± 1.238
8.859 ± 0.566
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.335 ± 0.104
5.097 ± 0.565
6.675 ± 1.695
6.189 ± 0.431
7.767 ± 1.178
6.796 ± 3.644
8.859 ± 0.997
1.82 ± 0.207
2.791 ± 1.124
4.126 ± 1.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here